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Citations for BioLegend :
1451 - 1500 of 7869 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... 1-16 Antibody (6E10) (used with 1:1000 dilution) was purchased from BioLegend. Anti-S6 Ribosomal Protein Antibody (used with 1:1000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Samples were washed 1 time then Zombie Green (Biolegend, Catalog #423111, 1:1000) was added to measure and gate on the % live HL-60 cells and anti-mouse/human CD11b PE (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were labeled with the following antibodies: PD-1 FITC (1:50, Biolegend), NKp46-PE (1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... CD279 (PD-1) BV510 (clone 29F.1A12, BioLegend, 135241, B342120, 1:100 dilution), CD223 (LAG-3 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-β-Amyloid 1-16 (6E10) (1:1000 dilution, cat# 803014, BioLegend), rat anti-CD68 (1:200 dilution ...
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bioRxiv - Bioengineering 2024Quote: ... Ly-6A/E (Sca-1) PE/Cy7 (clone D7, BioLegend; 1:1000 dilution), CD48 PerCP/Cy5.5 (clone HM48-1 ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were resuspended in FACS buffer (PBS, 1% FBS, 1 mM EDTA, 0.02% NaN3) and treated with anti-CD16/CD32 (clone 93, Biolegend 101302, 1:100) for 20 min on ice before staining.
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bioRxiv - Immunology 2024Quote: ... CD4-PE (in-house, GK1.5, 1:400), CD8α-A594 (in-house, 53-6.7, 1:400) and CD3χ-PE/Cy7 (BioLegend, 145-2C11, 1:1000) as well as CD45.2 in either BV421 (TR-A ...
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bioRxiv - Immunology 2024Quote: ... CD4-A594 (in-house, GK1.5, 1:400), CD8α-A647 (in-house, 53-6.7, 1:400) and CD3χ-PE/Cy7 (BioLegend, 145-2C11, 1:1000) and scored as CD3χ + cells ...
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bioRxiv - Cancer Biology 2024Quote: Mice with a human engraftment above 5% measured through flow cytometry using mCD45 (eFluor 450, eBiosciences, 1:100), hCD7 (PE, eBiosciences, 1:25) and hCD45 (Alexa Fluor 647, BioLegend, 1:25) were started on treatment which consisted of 0.50 mg kg−1 of vincristine (1 mg ml−1 injection ...
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bioRxiv - Immunology 2022Quote: ... and SCT Dd or SCT Dd-CD4 cell surface expression was regularly assessed by flow cytometry (clone 34-5-85 and 34-2-12, Abcam and Biolegend, respectively). Transfectants were FACS-sorted (FACSDiva and FACSARIAIIIU cell sorter ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were first blocked with 5% BSA for 30 min and stained with Alexa Fluor® 647 anti-human CD161 antibody (clone HP-3G10; BioLegend) at 10 μg/ml in blocking solution for 60 min ...
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bioRxiv - Molecular Biology 2020Quote: Freshly isolated CD3+ T cells were labeled using CFSE working solution (5 μM) using the CFSE Division Tracker Kit (Biolegend, USA). T cells were incubated for 20 min at 37 ◻ and quenched staining with culture medium ...
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bioRxiv - Cell Biology 2021Quote: ... Membrane was blocked in 5% milk followed by probing with either α-HA Mouse Monoclonal (Catalogue #HA.11; Biolegend, SanDiego, CA) or α-Rsp5 Rabbit Polyclonal [84] antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples that were stained for cell surface staining only were incubated for 5 min on ice in 7-AAD (Biolegend, #420404). Samples that were stained for intracellular markers were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... The suspensions were then spun at 300 x g for 5 minutes and resuspended in red blood cell (RBC) lysis buffer (Biolegend, 420302) for 5 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membrane was next added to 5% nonfat milk in TBST containing horseradish peroxidase-conjugate goat anti-mouse IgG1 secondary antibodies (Biolegend 405306) diluted at 1:5000 and incubated on a horizontal rocker for 60 minutes ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Immunology 2020Quote: ... cells were pelleted for 5 minutes at 500xg and resuspended in 50 µL Intracellular Staining Perm Wash Buffer supplemented with 5 µL of TruStain FcX (Biolegend, 422302) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The frozen tissue sections were treated with blocking solution (5% normal donkey serum in PBS) and then stained with FITC-conjugated anti-Ly6G antibody (BioLegend, 1A8). Nuclei were stained by DAPI ...
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bioRxiv - Immunology 2020Quote: ... were resuspended in 100μl protein blocking solution with 5 μl fluorescent conjugated antibodies, CD8 (Biolegend Cat# 100714, CD45 (Biolegend Cat# 103130), CD68 (Biolegend Cat# 137004) ...
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bioRxiv - Biophysics 2023Quote: ... The gel substrate for T cells had a Young’s modulus of 1.2 kPa and was coated with 5 μg/mL each of anti-CD3 (OKT3; 317326; BioLegend, CA, USA) and anti-CD28 (CD28.6 ...
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bioRxiv - Molecular Biology 2023Quote: ... Collected cell populations were immediately seeded in tissue culture at a concentration of 2x105 cells per 1.9 cm2 in DMEM containing 5% FBS and 10 ng/mL TGFβ (Biolegend Cat 763102) or 500 nM Prostaglandin F (Cayman Chemical Cat 16010).
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Microbiology 2023Quote: ... Each well was blocked using 50 μL of FACS buffer (PBS + 5% FBS) + 2.5 μL Fc receptor (BioLegend, Cat No. 422302) block per well and then 150 μL FACS buffer was added and spun down ...
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bioRxiv - Bioengineering 2024Quote: Resting T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 1 x 106 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named ‘FACS buffer’) together with 5 uL of TruStain FcX (BioLegend, 422301). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... followed by 5 µL/well of the antibody cocktail for extracellular staining: anti-CD3-BV421 (clone 17A2) (BioLegend, SanDiego, CA, USA) (1:200) ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellets were harvested and resuspended in 5 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 5 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) prior to run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) before run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... The fluorochrome-conjugated antibodies were as follows for flow cytometry: BV605 Rat Anti-Mouse CD4 (RM4-5/RM4-4; Biolegend #116027), PE-Cy™7 Rat Anti-Mouse CD8a (53-6.7 ...
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bioRxiv - Immunology 2024Quote: ... P14 T cells were stimulated with 100 ng/mL LCMV gp33 (KAVYNFATC) in the presence of 5 μg/mL Brefeldin A (BioLegend 420601) for 5 h at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... washed cells were resuspended in 100 μL of FACS buffer with 5 μL of PE anti-human TMPRSS2 antibody (BioLegend #378403) per million cells and incubated for 30 min at 4 °C in the dark ...
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bioRxiv - Immunology 2024Quote: ... for 20 minutes at 37°C and resuspended in cRPMI supplemented with 5 ng/mL recombinant mouse IL-2 (Biolegend #575404). 5×105 cells/well plated in a 96-well round-bottom plate ...
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bioRxiv - Cancer Biology 2024Quote: ... or after a 4-day incubation period at 37°C with 5% CO2 (Day 4) following 30 min antibody staining and additional 15 min Annexin V APC binding (Biolegend, 640920). The antibodies used were as follow ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice with 2 mL of PBS + BSA (400 g for 5 min) and run fixed (Biolegend fixation buffer) or unfixed prior to sorting and analysis ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with 2 mL SB (350 g for 5 min) and subsequently with 2 mL of 1X Tandem Stabilizer (Biolegend #421802). Cells were resuspended in 200 µL of 1X Tandem Stabilizer.
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bioRxiv - Developmental Biology 2024Quote: ... The supernatant was removed after centrifugation at 500 g for 5 min and the cell pellet was resuspended in red blood cell lysis buffer (BioLegend, 420301) for 10 min ...
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bioRxiv - Immunology 2022Quote: ... CD44 – PE-Cyanine7 (eBiosciences, # 25-0441-81; 1:600), CD4 – APC (eBiosciences, #17-0042-83; 1:200) and CD8a – APC-Cyanine7 (BioLegend, #100714; 1:400) combined with AZDye 488 alkyne/azide ...
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bioRxiv - Cell Biology 2024Quote: ... Flt3-PE (eBioscience,12-1351-82; 1:100), CD34-FITC (eBioscience, 11-0341-85; 1:25) and CD16/32-PE/Cy7 (BioLegend, 101318; 1:800), along with lineage antibodies in PE/Cy5 (Gr1 [eBioscience ...