5 Nitro 1H benzo de isoquinoline 1 3 2H dione citations for BioLegend

Quote: ... the cells were stained at the same time with the APC conjugated surface marker antibody CD45 after a 5 min blocking step with Human TruStain FcX solution (20x, BioLegend) diluted in 1 % BSA/PBS ...

Mechanistic insights into RNA binding and RNA-regulated RIG-I ubiquitination by TRIM25

Kevin Haubrich, et al., bioRxiv - Molecular Biology 2021

Quote: ... The cleared lysates were treated with 8 ng/ml of RNase A and 2U/ml Turbo DNase for 5 min at 37° C and immunoprecipitated for two hours using anti-HA magnetic beads (901502, BioLegend). The beads were washed three times with lysis buffer and three times with PNK buffer (50mM Tris-HCl ...

Robust and Functional Immunity up to 9 months after SARS-CoV-2 infection: a Southeast Asian longitudinal cohort

Vo Hoa Thi My, et al., bioRxiv - Immunology 2021

Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...

Control of Neutrophil activation through Semaphorin 7A-Plexin C1 is essential for immune defense during pulmonary sepsis

Tiago Granja, et al., bioRxiv - Cell Biology 2021

Quote: ... Ly6G-PE-Cy7+ and Ly6G- APC-) and intravascular PMNs (CD45-PerCP-Cy5·5+; Ly6G-PE-Cy7+ and Ly6G-APC+) (all antibodies from BioLegend) by flow cytometry (FACS Canto II ...

Design-of-Experiments In Vitro Transcription Yield Optimization of Self-Amplifying RNA

Karnyart Samnuan, et al., bioRxiv - Molecular Biology 2021

Quote: ... while the MDR1 replicons were stained with 5 μL of PE anti-human CD243 (ABCB1) antibody clone 4E3.16 (Biolegend, US). After staining for 30 min on ice ...

Inflammatory stress signaling via NF-kB alters accessible cholesterol to upregulate SREBP2 transcriptional activity in endothelial cells

Joseph W. Fowler, et al., bioRxiv - Cell Biology 2022

Quote: ... lung cells were brought to a single-cell suspension via collagenase incubation and then stained for flow cytometry at a concentration of 5×106 cells/mL with Cd31 (Biolegend) and 3μM ALOD4-47.

SOX15 regulates stem cell pluripotency and promotes neural fate during differentiation by activating Hes5

Eun-Bee Choi, et al., bioRxiv - Cell Biology 2022

Quote: ... cells were blocked with 5% bovine serum albumin (BSA) and 5% goat serum for 1hr at RT and incubated with primary antibodies (anti-βIII-tubulin antibody [BioLegend]) overnight at 4°C ...

An affinity-matured DLL4 ligand for broad-spectrum activation and inhibition of Notch signaling

David Gonzalez-Perez, et al., bioRxiv - Bioengineering 2022

Quote: ... at 0.5 μL/well for 5 hours and washed with PBS for detection of intracellular IFNγ using anti-hIFNγ APC antibody (Biolegend). The frequency of IFNγ positive cells was analyzed by flow cytometry (CytoFLEX II ...

Mechano-redox control of Mac-1 de-adhesion from ICAM-1 by protein disulfide isomerase promotes directional movement of neutrophils under flow

Alexander Dupuy, et al., bioRxiv - Biochemistry 2022

Quote: ... neutrophils (1×106 cells/mL) were added to each well in the presence of APC conjugated anti-CD11b antibody CBRM1/5 (BioLegend) at 1 μg/mL ...

Antibody-drug conjugates targeting CD45 plus Janus kinase inhibitors effectively condition for allogeneic hematopoietic stem cell transplantation

Stephen P. Persaud, et al., bioRxiv - Immunology 2020

Quote: Cytokine concentrations in culture supernatant or mouse plasma were measured with the LegendPLEX Inflammation Panel (13-plex) or the Mouse Th1 Panel (5-plex) per the manufacturer protocols (BioLegend). Quantification was done using LegendPLEX software v8.0 for Windows ...

CD56 regulates human NK cell cytotoxicity through Pyk2

Justin T. Gunesch, et al., bioRxiv - Immunology 2020

Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...

Transcriptomic profiling of human effector and regulatory T cell subsets identifies predictive population signatures

Barbara Höllbacher, et al., bioRxiv - Immunology 2020

Quote: ... CD4+CD25high Treg cells were enriched from PBMCs after staining with PE-cyanine 5 (PE-Cy5)-labeled anti-CD25 Ab (BioLegend), followed by positive selection using anti-PE and anti-Cy5 microbeads (Miltenyi Biotec) ...

T-cell Abca1 and Abcg1 cholesterol efflux pathways suppress T-cell apoptosis and senescence and increase atherosclerosis in middle-aged Ldlr^-/- mice

Venetia Bazioti, et al., bioRxiv - Immunology 2022

Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...

T-cell Abca1 and Abcg1 cholesterol efflux pathways suppress T-cell apoptosis and senescence and increase atherosclerosis in middle-aged Ldlr^-/- mice

Venetia Bazioti, et al., bioRxiv - Immunology 2022

Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...

Mitochondrial Transcription Factor A Regulates Foxp3+ T Cell Mediated Maintenance of CD4+ T Cell Landscapes and Immunological Aging

Kai Guo, et al., bioRxiv - Immunology 2022

Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...

An In Vitro Vascularized Micro-Tumor Model of Human Colorectal Cancer Recapitulates In Vivo Drug Responses

Stephanie J. Hachey, et al., bioRxiv - Cancer Biology 2020

Quote: ... Single tumor cells were resuspended to 5 × 107 cell/mL and stained with CD298-APC (10 µL/106 cells)(BioLegend) and Sytox Green (ThermoFisher ...

CoVaccine HT™ adjuvant potentiates robust immune responses to recombinant SARS-CoV-2 Spike S1 immunisation

Brien K. Haun, et al., bioRxiv - Immunology 2020

Quote: ... Splenocytes at (5 × 104 per well) were incubated with PMA (0.01 μM) /Ionomycin (0.167 μM) cocktail (BioLegend, San Diego, CA) as a positive control ...

Labshake search