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Citations for BioLegend :
1401 - 1450 of 7869 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... T-25 or T-75 flasks were coated with 5 µg/mL anti-CD3ε antibodies (clone 145-2C11; 100340, BioLegend) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes from uninfected or infected mice were incubated at 37C° in 5% CO2 for 4 h in medium containing Monensin and Brefeldin A solutions (Biolegend). Cells were then stained for surface antigens (TCRβ-PacificBlue and NK1.1-PE) ...
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bioRxiv - Immunology 2021Quote: Cell samples went through a series of centrifugations (400g, 5 minutes, at 4°C) and were stained with PE anti-CD45 (30-F11, BioLegend) in staining buffer (see flow cytometry section ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 50 µL/well of either 10 µg/mL (flat-bottom) or 5 µg/mL (round-bottom) anti-mouse CD3ε (BioLegend) in PBS (for stimulated wells) ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Immunology 2020Quote: ... for 5 minutes at room temperature prior to staining with a surface-stain cocktail containing the following antibodies purchased from BioLegend® (San Diego ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... followed by a 30 minute incubation with the TotalSeq-C antibody pool and 5 μl of a PE/Cyanine7 anti-human CD8 antibody (BioLegend) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5-10 x 106 cells were washed with PBS and stained with Zombie NIR Fixable live/dead dye (Biolegend, 423106) at 4 °C for 20 min ...
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bioRxiv - Immunology 2022Quote: ... isolated lymphocytes were first restimulated with 5 ng/mL phorbal 12-myristate 13-acetate and 500 ng/mL ionomycin in the presence of monensin (Biolegend) for 3.5 h ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Immunology 2024Quote: ... isolated naïve splenic B cells were cultured at a density of 0,5 x 106 cells/ml in the presence of 25 μg/ml LPS and 5 ng/ml IL-4 and stained with anti-CD138 (BioLegend). B cell proliferation was assessed by CellTrace Violet (Thermofisher ...
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bioRxiv - Immunology 2024Quote: ... for 40 min on ice or with biotinylated anti-MR1 antibody 26.5 (BioLegend, custom conjugation, used at 5 μg/ml) for 40 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed 5 times in 150 µL of PBST and incubated with 100 µL of streptavidin conjugated to horseradish peroxidase (HRP, BioLegend) diluted 1:2000 in PBS for 1 hour at RT ...
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bioRxiv - Bioengineering 2024Quote: T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Immunology 2023Quote: For Langerhans cells analysis, after centrifugation (450g, 5 min) cells were stained with anti-CD45 FITC (BioLegend, clone 30-F11), anti-EpCAM APCFire750 (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Pospho-GSK-3α/β and pospho-Akt were quantitated following 5 minutes of stimulation in transfected T cells fixed and permeabilized with fixation and permeabilization buffers (Biolegend) and stained with anti-pospho-GSK-3α/β (R&D systems ...
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bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... anti-CD25(Percp-Cy5.5, #302626), anti-CD69(APC, #310910), anti-mouse CD25(PE, #12-0251-83), anti-mouse CD69(FITC, #104506) from Biolegend, anti-human IgG(SureLight® PE ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 000-200 000 cells per sample were processed by blocking with 5 µl of Human TruStain FcX Blocking Solution (BioLegend) for 10 min at 4°C ...
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bioRxiv - Pathology 2023Quote: ... Ldlr−/− mice fed a chow diet or HC diet for 4 days were anesthetized using Isoflurane and injected intravenously with 5 μg anti-mouse TIM4-APC antibody (RMT4-54, BioLegend). They were then euthanized 5 minutes after injection by cervical dislocation and perfused through the portal vein with PBS and then with 4% ice-cold PFA ...
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bioRxiv - Immunology 2023Quote: ... lysis was performed at room temperature for 5 minutes in 1X RBC lysis buffer (10X RBC lysis buffer diluted in mqH2O, 420301, BioLegend) and reaction was stopped by diluting the 1X RBC lysis buffer with 35ml 1X DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and bone marrow were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed once with FACS buffer and pre-incubated with 5 μg/mL TruStain FcX anti-mouse CD16/32 antibody (clone 93, BioLegend) in 100 μL FACS buffer for 5 minutes on ice before immunostaining ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... PCLS were stained in 250 μl of 5 μg/ml Hoechst 33342 and 2.5 μg/ml AlexFluor 647-conjugated anti-EpCAM (Biolegend 118212) by incubating for 1 hr at 37°C ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulations were performed by first coating a 96-well plate with 5 μg/mL anti-mouse CD3 (Biolegend) in 50 μL/well PBS overnight the day before tissue harvest ...
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bioRxiv - Immunology 2024Quote: ... and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2, BioLegend, cat. 302933) in R10 medium [RPMI-1640 medium supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Bioengineering 2024Quote: ... for 5 min on ice and then stained with fluorochrome conjugated antibodies: CD19 (PerCP-Cy5.5, clone: 1D3, BioLegend, cat: 152406) CD95 (PE-Cy7 ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500 rpm for 5 min at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500 rpm for 5 min at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500 rpm for 5 min at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Immunology 2024Quote: ... 0.3 x 106 OT-I T cells were placed on flat-bottom 96 well plates coated with 5 µg/mL anti-CD3ε (clone 145-2C11; Biolegend) and stimulated with 1 µg/mL anti-CD28 mAb (clone 37.51 ...
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bioRxiv - Immunology 2024Quote: ... red blood cells (RBC) were eliminated by incubation of the cell pellet with 5 mL of RBC lysis buffer (BioLegend) for 5 min at RT ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 400 g for 5 minutes at 4°C and resuspended in cell staining buffer (CSB; Biolegend). The number of cells in the suspension was established using the CountessTM II cell counter (Invitrogen).
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bioRxiv - Bioengineering 2024Quote: ... After 48h of incubation at 37 °C and 5% CO2 the co-cultures were prepared for flow cytometry analysis by staining for CD3 (cat. # 317318, Biolegend) and viability (DAPI ...
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bioRxiv - Bioengineering 2024Quote: ... a staining solution was prepared by adding 5 µL of each fluorescent-conjugated antibody (anti-CD20-FITC, anti-CD45-APC, anti-CD56-APC-AF750, Zombie Violet; BioLegend) to FACS buffer ...
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bioRxiv - Bioengineering 2024Quote: ... for 5 mins on ice and stained with fluorochrome conjugated antibodies: CD19 (PerCP-Cy5.5, clone: 1D3, BioLegend, cat. Number 152406), CD95 (PE-Cy7 ...
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bioRxiv - Cell Biology 2024Quote: ... The antibodies were pre-conjugated to Protein A/G Dynabeads by incubating 100 µl of beads with 5 µg of either mouse Anti-HA (16B12 clone, BioLegend) or mouse IgG2b isotype control (MPC-11 clone ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used for immunostaining: rat anti-EpCAM-AF647 (Biolegend, clone G8.8, 1 µg ml−1, 1:250); rabbit anti-K5 (Covance ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were using 1:500 rabbit anti Abeta 1:500 (IBL, CAT # 18584) and 1:500 mouse anti SMI312 (Biolegend 837904).
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bioRxiv - Immunology 2023Quote: ... IL-6 (clone MP5-20F3, 1:100), TNF-α (clone MP6-XT22, 1:66) and CD80 (clone 16-10A1, 1:100) from Biolegend.
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bioRxiv - Immunology 2024Quote: ... Fluorochrome-conjugated antibodies (1:400 anti-EpCAM-AF594, 1:200 anti-MHC-I-AF647 and 1:400 anti-MHC-II-AF488 (BioLegend, USA)) were diluted in blocking buffer and 100µl of staining mix was placed on each section for 1h at RT ...
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bioRxiv - Cancer Biology 2021Quote: β1 integrin was immunoprecipitated with anti-β1 integrin (clone HMb1-1, BioLegend, #102202), or anti-active β1 integrin (clone 9EG7 ...
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bioRxiv - Microbiology 2021Quote: ... rat anti-mouse IgM-BV421 (clone RMM-1, 1:200, Biolegend, cat#406518), rat anti-mouse CD138-BV605 (clone 281-2 ...
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bioRxiv - Biophysics 2020Quote: ... astrocytes were labelled 1 hour at RT with anti-GFAP (1:150,644702, BioLegend) for staining GFAP ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated with 1 μg mL-1 PE-anti HA.11 (BioLegend) at 4 °C with rocking for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Antibodies against IgG1 (RMG1-1) and IgA (RMA-1) were obtained from BioLegend. For intranuclear staining ...