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Citations for BioLegend :
101 - 150 of 3486 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: THP-1 human macrophages were treated with mouse anti-human Fc block (Ref: 422302, BioLegend), for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend) ...
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bioRxiv - Pathology 2022Quote: ... human and murine Fc receptors were blocked using Human TruStain FcX (Biolegend, #422302, 1/20) and anti-mouse CD16/CD32 (FcBlock clone 2.4G2 ...
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bioRxiv - Immunology 2024Quote: ... Human cells were blocked by Human TruStain FcX Fc receptor blocking solution (#422301 from BioLegend). Flow cytometry analysis was performed using an AccuriC6 Plus platform ...
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bioRxiv - Biophysics 2021Quote: ... and then incubated with biotinylated anti-human CD3 antibody (OKT3) (BioLegend # 317319) at 1 ug/mL for 35 minutes ...
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bioRxiv - Immunology 2020Quote: ... and 50 ng/ml recombinant human M-CSF (BioLegend #574806) for 6 days to obtain monocytes derived macrophages ...
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bioRxiv - Immunology 2021Quote: ... 5ng/ml or 50ng/ml recombinant human G-CSF (BioLegend) or mouse G-CSF (BioLegend) ...
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bioRxiv - Microbiology 2022Quote: ... and FITC anti-human/mouse Granzyme B recombinant antibody (Biolegend). Samples were analyzed on the CytoFLEX LX flow cytometer (Beckman Coulter).
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bioRxiv - Cancer Biology 2022Quote: ... Human and mouse recombinant IFNα/β/γ(all from Biolegend) were used at a concentration of 100ng/ml.
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bioRxiv - Biochemistry 2020Quote: ... HA-Flag-tagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... human and mouse FC receptors were blocked using Human TruStain FcX (BioLegend, San Diego, CA, USA) and purified anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... human cells were blocked with Human TruStain FcX Fc Receptor Blocking Solution (Biolegend, #422302, 1:100) in FACS buffer and stained with anti-hCD19-PE-Cy7 and anti-hCD138-PE-Cy7 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked with human TruStain FcX (Biolegend, Table S6) for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... APC-conjugated anti-human IgG Fc (clone HP6017) was from Biolegend. For analysis of CD47 expression on human PBMCs ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD4 (BV711 ...
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bioRxiv - Immunology 2022Quote: ... and incubated with Fc receptor blocking solution (Human TruStain FcXTM; BioLegend) before surface staining with fluorochrome-conjugated antibodies to CD3 (APC-Cy7 ...
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bioRxiv - Genomics 2020Quote: ... and incubated with Fc Receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes prior to staining with TotalSeq C antibodies (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Anti-human IgG Fc allophycocyanin (APC) (BioLegend, San Diego, CA, USA) was added as a secondary antibody at 10 μg/mL and the plate was incubated at 4°C for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cells were incubated with Fc blocking reagent (Biolegend Human Trustain FcX) for 15 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... The remaining cells underwent anti-human Fc-blockade (Biolegend, TruStain FcX) for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: Single cell suspensions were blocked with human BD Fc Block (BioLegend) for 5 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibody used was anti-human IgG Fc antibody (Biolegend, 410720). All the respective isotype antibodies used were procured and used as per the vendor’s instructions.
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bioRxiv - Immunology 2022Quote: ... for fluorescence microscopy: α-human HLA-A,B,C (W6/32, biotinylated, Biolegend), streptavidin-AlexaFluor-647 (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for 25 min with biotinylated anti-human MMR (321118, BioLegend), washed ...
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bioRxiv - Immunology 2021Quote: Recombinant human ARG1 was obtained from Biolegend (San Diego, CA, USA), recombinant murine Arg1 was obtained from Cloud-Clone Corp. ...
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bioRxiv - Immunology 2021Quote: ... and mouse recombinant PE anti-human/mouse TLR2 (clone QA16A01; Biolegend) were used.
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bioRxiv - Immunology 2022Quote: ... and recombinant human transforming growth factor beta-1 (TGF-β1; Biolegend) were used at final concentrations of 10 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... and 1 U/mL of recombinant human IL-2 (BioLegend, #589102) in 200 μL of complete culture medium (see above for media components) ...
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bioRxiv - Immunology 2023Quote: ... were coated with 1.54 μg/mL human recombinant VCAM-1 (Biolegend) and/or 1.14 μg/mL human recombinant ICAM-1 (R&D system ...
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bioRxiv - Bioengineering 2023Quote: ... and 500 IU/ml of recombinant human IL-2 (Biolegend, 589104). Stimulated T cells were co-transduced via spinoculation at 931xg ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1000 U/ml of human recombinant IFN-γ (BioLegend), 20 ng/ml of EGF (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Bound biotinylated protein was detected by adding Avidin-HRP (405103, BioLegend) diluted 1:2000 in TSM buffer ...
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bioRxiv - Immunology 2023Quote: ... All recombinant proteins and compounds were purchased from Biolegend, USA ...
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bioRxiv - Bioengineering 2024Quote: ... Magnetic beads displaying recombinant IL-6 protein (BioLegend #570806) were prepared by washing 70 uL of Tosyl-activated M-280 magnetic dynabeads (ThermoFisher #14203) ...
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bioRxiv - Bioengineering 2023Quote: ... Binding of T cell-secreted SiRPα-Fc on T cell CD47 was assessed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding was detected using an APC Anti-Human IgG Fc (Biolegend) at a 1:100 dilution incubated for 1 hr with the bacteria ...
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bioRxiv - Bioengineering 2022Quote: ... then stained with APC anti-human IgG Fc Antibody clone HP6017 (Biolegend). Samples were acquired on a BD FACSymphony A5 analyzer and data were analyzed using FlowJo (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... Pooled barcoded samples were treated with FC-block (Human TruStain FcX, Biolegend) for 10 minutes then surface antibody stained for 30 minutes in cell staining medium (PBS with 0.5% BSA and 0.02% sodium azide) ...
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bioRxiv - Cell Biology 2019Quote: ... Fc receptors were blocked with human IgG antibody at 1:50 (Biolegend) in PBS (pH 7.2 ...
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bioRxiv - Microbiology 2021Quote: ... Fc receptors were blocked using Human TruStain FcX (BioLegend, #422302, 1/20) diluted in cold FACS buffer (1x PBS supplemented with 0.5% BSA and 2 mM EDTA) ...
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bioRxiv - Genomics 2021Quote: ... and incubated with Fc-receptor blocking antibody (Human TruStain FcX, BioLegend 422302) in homogenization buffer for 20 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: ... followed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:100 human TruStain FcX (Fc Receptor Blocking Solution) (BioLegend, Cat: 422301) and 1:100 TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with Fc receptor blocking antibodies (Human TruStain FcX, Biolegend) 1:20 in Cell Staining Buffer (CSB ...
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bioRxiv - Bioengineering 2023Quote: ... followed by staining with labeled goat anti-human IgG Fc (HP6017, Biolegend) at 4°C for 30min ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Immunology 2023Quote: ... Soluble His6-tagged M6G at varying concentrations was incubated for 10 min with HRP-conjugated mouse anti-His antibody (BioLegend) diluted 1:2000 in 0.1% BSA/TBST ...
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Accumulation of TCR signaling from self-antigens in naive CD8 T cells mitigates early responsivenessbioRxiv - Immunology 2023Quote: ... the following biotinylated anti-mouse or anti-mouse/human antibodies were purchased from BioLegend: CD4 (clone RM4-5) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... a final concentration of 5ng/ml of recombinant human TGFβ1 (BioLegend, #580702) was added to the culture media and treated for 24~48 hours ...
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bioRxiv - Immunology 2022Quote: ... 100ng/ml of recombinant human (rh) IL-8 (Biolegend, San Diego, CA) were added to the apical side of the enteroid monolayers (76) ...