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Citations for BioLegend :
401 - 450 of 3486 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... recombinant BMP6 (rBMP6) (Biolegend, USA), recombinant Follistatin (rFST ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant Follistatin (rFST) (Biolegend, USA) or vehicle control were added to the desired concentration whilst still preconfluent (approximately 24 hours before confluency) ...
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bioRxiv - Cancer Biology 2019Quote: ... washed once with PBS at concentration 2×10^6/mL in RPMI1640 containing 10% FBS and 100U/ml of recombinant human IL-2 (BioLegend, San Diego, CA). After 72h ...
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bioRxiv - Biochemistry 2022Quote: Moles of product formed in hydrolysis reactions used for ACE2 activity and specificity profiling were determined by comparing area under the curve for LC chromatograms corresponding to these reactions to areas under the curve observed for control reactions in which > 95% input substrate conversion was achieved by incubation of substrate with 25 nM recombinant human ACE2 commercial standard (BioLegend, San Diego, CA) for between one and four hours pending peptide substrate ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with M-CSF (100ng/ml) Recombinant Human M-CSF (Macrophage-Colony Stimulating Factor, BioLegend cat. 574806, San Diego, CA, USA) for 5 min ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated antibodies were stained with Strepatvidin-BV421 (Biolegend). Staining with propidium iodide (PI ...
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bioRxiv - Immunology 2020Quote: ... or biotinylated anti-mouse cKit (clone 2B8, BioLegend) with sAV-SAP in a 1:1 molar ratio for 15 minutes at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... Then a biotinylated anti-IL-2 Ab (BioLegend) at a concentration of 0.25 μg/ml was added and after washing ...
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bioRxiv - Immunology 2021Quote: ... and biotinylated anti-CD45 antibody (BioLegend, 1:100). Immunohistochemistry using monoclonal mouse anti-β-catenin (BD Transduction Laboratories ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated anti-IFNγ R46A2 (0.5 μg/mL, Biolegend) was prepared in blocking solution and added to the plate ...
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bioRxiv - Neuroscience 2023Quote: ... biotinylated anti-HA (mouse, 1:500, BioLegend 901505), anti-CD68 (rat ...
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bioRxiv - Immunology 2022Quote: ... and 0.5µg of biotinylated RBD with BV421 (BioLegend) were incubated for 30 minutes in the dark to generate fluorochrome-linked biotinylated tetramers ...
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bioRxiv - Immunology 2022Quote: ... biotinylated mouse anti-rat IgG1 (Biolegend, Cat. 407404) or biotinylated mouse anti-rat IgG2a (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... rat biotinylated anti-CD45 (Biolegend, 5530, 1:200), rabbit anti-RXRα (D6H10 ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated lectins were detected using PE-Streptavidin (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Biotinylated Spike was conjugated to streptavidin-FITC (BioLegend) and streptavidin-PE (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Developmental Biology 2021Quote: ... and then Fc blocked (BioLegend, 101320), before staining with an antibody cocktail containing anti-MPO (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... and Fc receptor blocking solution (Biolegend), before staining with fluorochrome-conjugated anti-human antibodies specific to anti-CD3 (UCHT1)/anti-CD45 (2D1) ...
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bioRxiv - Neuroscience 2022Quote: ... After fragment crystallizable (Fc) blocking (Biolegend), cells were stained for surface antigens (Extended Data Table 4) ...
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bioRxiv - Cancer Biology 2020Quote: ... After adding Fc receptor blocker (BioLegend), cells were labeled overnight at 4°C with a cocktail of antibodies recognizing chromatin modifications or intracellular components ...
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bioRxiv - Immunology 2020Quote: ... and incubated with FC block (BioLegend), followed by tetramer incubation (25μg/ml ...
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bioRxiv - Immunology 2022Quote: ... 5µg/ml CD80-Fc (555404, Biolegend), and 2µg/ml PD-L1-Fc (758206 ...
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bioRxiv - Immunology 2022Quote: ... stained with Fc receptor block (BioLegend) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... After Fc blocking (TruStain FcX; BioLegend), cells were stained with a metal-conjugated surface antibody cocktail (Table 2) ...
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bioRxiv - Cancer Biology 2023Quote: ... performed with Fc blocking (422302, BioLegend), and labelled with anti-CD138 (142508 ...
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bioRxiv - Immunology 2024Quote: ... and incubated with Fc Block (Biolegend) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescently-tagged anti-mouse antibodies (BioLegend, San Diego, CA) were used to label cell surface markers for flow cytometry analysis ...
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bioRxiv - Immunology 2023Quote: ... and 7.5 nM of either a) tail-biotinylated cognate pMHC complex or b) biotinylated Armenian hamster anti-CD3ε antibody (clone 145-2C11, Biolegend 100304). ICAM-1 and pMHC proteins were expressed and purified as described (Wang et al. ...
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bioRxiv - Immunology 2021Quote: ... Samples were thawed and proteins quantified using a LEGENDplex Human Proinflammatory Chemokine Panel immunoassay (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
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bioRxiv - Molecular Biology 2020Quote: ... Organoids were cultivated in growth medium as previously described (61) with minor changes (50% mouse Wnt3a-conditioned medium, 50ng/mL mouse Wnt3a recombinant protein (Biolegend, USA), 15% mouse R-spondin1-conditioned medium ...
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bioRxiv - Immunology 2020Quote: ... APC-anti-his tag antibody (Biolegend, clone: J095G46)) ...
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bioRxiv - Biochemistry 2022Quote: ... and Purified anti-His Tag antibody (BioLegend 652502) for 1 hour in blocking buffer shaking at rt ...
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bioRxiv - Microbiology 2023Quote: ... Phycoerythrin (PE) anti-His tag antibody (BioLegend 362603) was added to the cell suspension at a dilution of 1:20 and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... and mouse anti-His (1:2500, 362601, BioLegend) were diluted in 5% BSA in 1× PBS + 0.2% Tween20 for blotting ...
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bioRxiv - Bioengineering 2021Quote: ... biotinylated Flex-T HLA-A*02:01 monomers (BioLegend), using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... biotinylated anti-mouse IL-1RI (clone: JAMA-147, BioLegend) at 1:100 ...
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bioRxiv - Microbiology 2021Quote: ... was used to detect biotinylated antibodies 7E9 (BioLegend, UK) and 7G6 (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... and the biotinylated anti-CD20 antibody (BioLegend, CA, USA). Purified CD20+ cells ...
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bioRxiv - Cancer Biology 2022Quote: ... biotinylated Flex-T HLA-A*02:01 monomers (BioLegend), using a modified version of the commercial protocol ...
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bioRxiv - Bioengineering 2022Quote: ... and biotinylated anti-mouse IL-1RI (JAMA-147, BioLegend) at 1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... and biotinylated anti-HA antibodies (BioLegend, #901505, 1:300) at 37°C for 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... and biotinylated anti-HA antibodies (BioLegend, #901505, 1:300) at 37°C for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... and biotinylated anti-HA antibodies (BioLegend, #901505, 1:300) at 4°C for overnight or at 37°C for an hour ...
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bioRxiv - Immunology 2022Quote: ... or biotinylated mouse anti-rat IgG2a (Biolegend, Cat. 407504) was added to the wells and the plate incubated for 1h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated antibodies were detected using streptavidin-HRP (Biolegend #405210).
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bioRxiv - Immunology 2023Quote: ... Eosinophils were column-purified with biotinylated anti-SiglecF (BioLegend), followed by anti-biotin MicroBeads then magnetic separation with MS columns according to manufacturer’s instructions (Miltenyi) ...
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bioRxiv - Cell Biology 2020Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...