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Citations for BioLegend :
251 - 300 of 3729 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: 100µl bone marrow were blocked with 2µl Fc Receptor Blocking Solution (Human TruStain FcX™, (422301, Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... then incubated with 0.5 ml of human TruStain FcX Fc receptor blocking solution (Biolegend, San Diego, CA) for 10 minutes.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in MACS buffer and incubated with PE–anti-human FC antibody (Biolegend, M1310G05) in MACS buffer for 30 min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as the primary antibody and rat anti-human IgG Fc antibody conjugated to APC (BioLegend) was used as the secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc-block was performed by incubating cells in a dilution of Human TruStain FcX (#422302, BioLegend, USA) and mouse TruStain FcX (#101320 ...
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bioRxiv - Genomics 2023Quote: ... The PBMC pellet was resuspended and cells were stained with Human TruStain FcX Fc Blocking Reagent (BioLegend) for 10 min at 4°C and then with fixable viability dye DAPI (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Bioengineering 2023Quote: ... The 5x diluted streptavidin-coated nanovial suspension was then incubated with an equal volume of 71.5 μg/mL of biotinylated anti-human VEGF antibody (Biolegend) for 30 minutes at room temperature on a tube rotator and washed ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Microbiology 2022Quote: ... Endotoxin-free recombinant mouse IL-1α and IL-1β proteins were purchased from BioLegend.
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bioRxiv - Immunology 2024Quote: Antibodies for reconstitution analysis of HIS mice (all antibodies are anti-human): CD45-Pacific Blue (Biolegend, HI30, Cat. 304029), CD3-PE (Biolegend ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice after the primary stain and incubated with PE–anti-human Fc antibody (Biolegend, 410708) in MACS buffer for 20 min on ice ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Immunology 2019Quote: ... an Fc block step was performed by resuspending cells in 50μl of Fc block prepared in PBS/5%BSA (room temperature) using 5μl of Human Trustain FcX (Biolegend) per sample i.e ...
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bioRxiv - Immunology 2024Quote: ... Cells were blocked for 15 min with 10 µL of human Fc receptor blocking solution (TruStain FcXTM; BioLegend). To minimize the interaction of polymeric dyes and improve discrete fluorochrome readout ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed with cold PBS and PE-labeled anti-human IgG Fc secondary antibody (Biolegend, 41070) was added and incubated for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution, Cat# 422302, BioLegend) and incubated for 30 min at RT ...
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bioRxiv - Immunology 2023Quote: ... P008_108)52 for 1h at 4°C followed by PE-rat anti-human IgG Fc (1:200, Biolegend) for 45 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... For comparison of resident vs non-resident populations tonsil samples were divided into CD69+ and CD69- subsamples using biotinylated anti-human CD69 antibody (FN40, Biolegend), and separation was performed using anti-Biotin Microbeads (Milenyi ...
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bioRxiv - Microbiology 2020Quote: ... anti-His PE (Biolegend), and human Hashtag 3 (Biolegend ...
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bioRxiv - Systems Biology 2020Quote: ... respective concentrations (ranging from 25 ng/mL to 100 ng/mL) of recombinant human IL-8 (Biolegend, 715404) and/or recombinant human CCL2 (Peprotech ...
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bioRxiv - Microbiology 2024Quote: ... Activation of MAPK and NF-κB signaling pathways was triggered using human recombinant TNF-α (BioLegend, Cat. 570102). Detailed information concerning experimental procedure is given in supplementary material.
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bioRxiv - Immunology 2022Quote: Biotinylated RBD protein (Acro Biosystems, cat# SPD-C82E9) was complexed with fluorochrome-conjugated streptavidin APC (BioLegend, cat# 405207). Lymph node cells from FNA were incubated with the RBD-fluorochrome complex and subsequently stained with aqua live/dead dye (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Non-specific binding was blocked by incubating cells with human anti-Fc Receptor antibodies (TrueStain, Biolegend, Cat. No. 101320) or anti-mouse CD16/32 Fc block (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... low endotoxin (Super affinity) (Acro) followed by a secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend) and RFP expression ...
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bioRxiv - Pathology 2022Quote: ... cell suspensions were centrifuged again at 300 g for 5 min and then preincubated with 2.5 µg/ml Fc blocker (156604 for mouse, 422302 for human, BioLegend) on ice for 10 min ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... In all assays 1 x 106 cells were first incubated with Human TruStain FcX (Fc Receptor Blocking Solution, Biolegend) or anti-human CD32 (FcγRII Blocker ...
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bioRxiv - Genomics 2021Quote: ... B cells were incubated with viruses for 2 hr and then stained with Human TruStain FcXTM (Fc Block, BioLegend), CD19-APC (clone HIB19 ...
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bioRxiv - Immunology 2022Quote: After thawing cells were washed and Fc receptors were blocked with TruStain FcX (mouse or human as appropriate; Biolegend) for 5 minutes at room temperature before staining with diluted antibodies (Supplemental Tables 2 ...
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bioRxiv - Immunology 2022Quote: All surface staining was done in the presence of Human Fc block and Monocyte Blocker (Biolegend, San Diego, CA). Following staining ...
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bioRxiv - Immunology 2022Quote: ... low endotoxin (Super affinity) (Aero) followed by a secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend). For cytotoxicity assay analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells were combined into a composite sample before incubation in Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and staining for surface antigens was performed following Fc receptor blockade with Human TruStain FcX (BioLegend). Central/effector memory CD4+ T cells were defined as live/CD16-/CD14-/CD123-/CD56-/CD11c-/CD19-/CD4+ > excluding CD25hiCD127lo > CD45RA-/CD27+ ...
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bioRxiv - Immunology 2023Quote: ... CD141 BV605(Clone:M80 Cat:344117) in the presence of human anti-Fc Receptor antibodies (TrueStain; Biolegend; cat. no. 101320). cDCs1 were FACS-sorted as CD45+Lin- (Lin:CD3 ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) with Fc blocked using human TrueStain FcX (Biolegend) and incubated in the dark for 10 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in FACS buffer (PBS with 0.5% BSA and 2 mM EDTA) and Fc blocked using human TrueStain FcX (Biolegend). For cell surface staining ...
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bioRxiv - Cell Biology 2023Quote: ... Osaka University) were pre-incubated with anti-human-Fc secondary antibodies fused to APC (BioLegend, #410712, dilution 1:100) for 1 h in 1% BSA in PBS ...
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bioRxiv - Immunology 2019Quote: ... Biotinylated mouse anti IgG2A (Biolegend) served as the isotype control ...
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bioRxiv - Immunology 2020Quote: ... CD8 – biotinylated (clone53-6.7, BioLegend), CD19 (clone1D3 ...
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bioRxiv - Immunology 2022Quote: ... biotinylated CD21/35 (7E9, Biolegend). The following secondary Abs were used for staining ...
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bioRxiv - Immunology 2024Quote: Biotinylated isotype control antibodies (BioLegend) were used to produce control ADCs ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated CD71 (Biolegend, clone R17217), PD1-PerCP/Cy5.5 (Biolegend ...
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bioRxiv - Immunology 2021Quote: 600 million cells from human tonsils were resuspended in Miltenyi magnetic-activated cell sorting (MACS) buffer and stained with biotinylated anti-human CD19 (clone: HIB19; BioLegend, #555411) on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: ... mouse ICAM-1-Fc and VCAM-1-Fc were from BioLegend. Recombinant mouse MAdCAM-1-Fc was from R&D Systems (Minneapolis ...