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Citations for BioLegend :
1401 - 1450 of 7287 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Slides were double immunostained by incubating overnight at 4°C with a mix of anti-CD4 conjugated with PERCP (BioLegend, San Diego, CA, USA) and anti IL-10 conjugated with PE (BD Biosciences Pharmingen ...
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bioRxiv - Immunology 2021Quote: ... Cells were suspended in FACS buffer and stained for 30 min at 4 °C with the desired antibody cocktails (BioLegend, San Diego, CA, US) of APC-Fire 750-anti CD11b (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 million cells for 30 min at 4 °C (Cat. No. 394601, 394603, 394605 and 394661, 394663, 394665, respectively, Biolegend, San Diego, CA, USA). After 3 washes with PBS with 0.05 % BSA respective cell suspensions were mixed prior to single-cell RNA library preparation ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 20 minutes at 4 °C using a Cyto-Fast™ Fix/Perm Buffer Set (426803, BioLegend; 100 μL/tube). After being washed and resuspended with Cyto-Fast™ perm wash solution ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were stained (30 min, 4°C in the dark) with CD11b (Clone: REA713; Miltenyi or Clone: M1/70; Biolegend, San Diego, CA, USA), CD14 (Clone ...
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... The slides were washed with PBS and incubated for 4 hours at room temperature with rat anti- mouse Ly6G (cone 1A8, BioLegend, San Diego, CA, USA). Slides were washed with PBS and incubated separately with Alexa Fluor 568 goat anti-rabbit (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and staining with Alexa 647 labeled anti-granzyme B (clone GRB11, Biolegend. All functional assays were performed in triplicate ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor 647 anti-human HLA-A,B,C (W6/32, Biolegend), and anti-SARS-CoV-2 Spike Glycoprotein S1 antibody (Abcam ...
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bioRxiv - Immunology 2020Quote: ... B cells and T cells were detected using anti-B220-AF488 (Biolegend) and anti-CD3-AF647 respectively (BD Bioscience) ...
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bioRxiv - Immunology 2020Quote: ... perforin (dG9, BB755, BD Biosciences, or B-D48, Brilliant Violet 421, Biolegend), T-bet (4B10 ...
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bioRxiv - Immunology 2021Quote: ... B cells were stained with Zombie Aqua™ Fixable Viability Kit (Biolegend) and blocked with FcR Blocking Reagent (Miltenyi Biotec ...
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bioRxiv - Genomics 2022Quote: ... Cells were then stained with a cocktail of TotalSeqTM-B antibodies (BioLegend) previously centrifuged at 14,000g for 10 minutes (Supplementary Table S2b) ...
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bioRxiv - Immunology 2022Quote: ... GC B cells were sorted for the phenotype B220+ (BV711: BioLegend 103255), CD95+ (PE-Cy7 ...
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bioRxiv - Molecular Biology 2023Quote: Each sample was incubated individually with 0.025μg TotalSeq-B hashtag antibodies (BioLegend, cat# ...
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bioRxiv - Immunology 2023Quote: ... and 200 ng/mL recombinant human B cell-activating factor (BAFF, BioLegend).
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bioRxiv - Immunology 2023Quote: ... and granzyme B were measured using bead-based flow cytometry (LegendPlex, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Sorted populations were labeled with TotalSeq-B Anti-Human Hashtag antibodies (Biolegend), and 3,000 cells from each of the seven sorted populations were combined for each donor and submitted to MSKCC’s Integrated Genomic Operation core facility for 10x Genomics library prep and 3’ Gene Expression and 5’ Feature Barcode sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 ug each of the 10 TotalSeq-B oligo-tagged antibodies (BioLegend) listed in Key Resources Table were added and cell pellets were suspended ...
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bioRxiv - Immunology 2024Quote: ... anti-granzyme B-Alexa Fluor 647 (Clone GB11) (BioLegend, SanDiego, CA, USA) (1:100 ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed by 7-AAD staining per the manufacturer’s instructions (Biolegend). For analyzing DNA/RNA content ...
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bioRxiv - Microbiology 2020Quote: ... resuspended in PBS at 106 cells/ml and incubated with 7-AAD (Biolegend) for 5 minutes before acquisition ...
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bioRxiv - Genomics 2022Quote: ... 4th passage HMECs were stained with anti-human CD133-PE (Biolegend, clone 7) and anti-human CD271 (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with 7-AAD (BioLegend, San Diego, CA, Cat. No. 420403) in FACS buffer (1% bovine serum albumin and 0.05% sodium azide in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Cell viability was assessed by 7-AAD staining per the manufacturer’s instructions (Biolegend). Flow cytometry data were acquired on Novocyte (ACEA Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Viability was assessed by staining with 7-AAD Viability Staining Solution (BioLegend, 420404). For intracellular DNA-PKcs flow ...
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bioRxiv - Immunology 2022Quote: ... anti-mouse F4/80 (APC-Cyanine® 7, BioLegend, clone BM8, cat#123118), anti-mouse TCRβ (BUV737® ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as 7-AAD Viability Staining Solution (420404) (Biolegend, San Diego, CA). Lymphoid populations were characterized using APC anti-mouse CD3 (100236 ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated with fluorescence APC-Cyanine-7-labeled anti-CD11b antibody (BioLegend, 101225) in the dark for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... 10mM HEPES),and stained at 4°C for 25 minutes using the following cell surface antibodies: CD14 Pacific Blue (clone M5E2, BioLegend cat# 301815, 2:100 dilution); CD19 Pacific Blue (clone H1B19 ...
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bioRxiv - Neuroscience 2023Quote: The single-cell suspension was stained at 4°C for 15 minutes with antibodies recognizing lymphocytes and endothelial cells (anti-CD45/CD31; BioLegend Way, San Diego, CA, USA) and labeling live cells (CellTrace calcein green ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking of nonspecific binding was induced in the cell suspensions via incubation for 10 min at 4°C with anti–CD16/CD32 antibody (cat # 101320, BioLegend, clone 93, 5 ng/μl). This was followed by staining for 30 min at 4°C with the appropriate antibodies ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD3 PE, Life Technologies; CD8 FITC, Life Technologies; CD11b AF700, Life Technologies CD19 PerCP-Cy5.5, BioLegend; C33 BV605, BioLegend; CD56 BV711, BD). After 30min ...
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bioRxiv - Immunology 2021Quote: Antibodies against HLA-A*02 and HLA-B*07 were purchased from BioLegend and BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Cell suspensions were labeled with TotalSeq Series B hashtag oligonucleotide-labeled antibodies (BioLegend). Single-cell RNA-seq (scRNA-seq ...
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bioRxiv - Immunology 2022Quote: ... B cells were then stained with FITC anti-human CD19 antibody (BioLegend, 392508), FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Physiology 2022Quote: ... The cell pellet was resuspended with anti-CD172a/b (SIRPα/β, 423107, Biolegend) and anti-CD90 (Thy-1 ...
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bioRxiv - Immunology 2022Quote: ... for fluorescence microscopy: α-human HLA-A,B,C (W6/32, biotinylated, Biolegend), streptavidin-AlexaFluor-647 (Life Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... B cells from each sample were then labeled with hashtag multiplexing antibodies (BioLegend) according to the manufacture’s protocol and pooled together ...
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bioRxiv - Immunology 2020Quote: ... CD19+ CD20+ IgD− B cells were incubated with Total-seq C antibodies (Biolegend) for 30 min and washed 3 times ...
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bioRxiv - Cell Biology 2021Quote: ... Granzyme B and perforin were determined by ELISA kits (ABclonal, Biolegend, and Abcam) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... and incubated with proper Total Seq B antibodies using recommended protocol from Biolegend. Cells were washed twice with PBS-0.5%BSA solution and mixed them together aiming at even cell numbers for each cell line ...
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bioRxiv - Cell Biology 2022Quote: ... and then stained with 50x diluted TotalSeq-B cell feature barcode antibody (BioLegend, San Diego ...
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bioRxiv - Immunology 2022Quote: ... Then isolated B cells were labeled with biotinylated anti-CD45.2 antibody (BioLegend, 109803), and then depleted with the anti-biotin Microbeads Ultrapure (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... and sCD40L were measured using the human B cell premix kit from Biolegend Legendplex™ using manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-human Perforin conjugated to PE/Cyanine7 (clone B-D48, Biolegend, 353315), mouse anti-human TNF-α conjugated to PE/Dazzle 594 (clone MAb11 ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated with TotalSeq B™ Universal cocktail (BioLegend; Cat. 399904) in Cell Staining Buffer (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... and a TotalSeq-B anti-mouse hashtag antibody specific to treatment group (Biolegend; Normal Brainstem:155843 ...
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bioRxiv - Immunology 2020Quote: ... each sample was stained with one unique hashing antibody purchased from BioLegend: HTO1 (M1/42 ...
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bioRxiv - Neuroscience 2022Quote: ... and treated with one application of red blood cell lysis buffer (Biolegend). After a final wash step in PBS ...
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bioRxiv - Immunology 2023Quote: ... One aliquot was stained with 10 μM Tag-it Violet (BioLegend, #425101) diluted in HBSS for 5 minutes on ice at a cell concentration of 1 million cells per milliliter ...