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Citations for BioLegend :
1601 - 1650 of 7287 citations for 7H Pyrazolo 4 3 d pyrimidin 7 one 1 4 dihydro 3 b D ribofuranosyl oxime monohydrochloride 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then processed for single cell RNAseq as described in (Peng et al., 2019) using Total Seq B antibodies and the recommended protocol from Biolegend. Briefly ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Immunology 2022Quote: ... Cat# 137024), granzyme B (QA18A28, Cat# 396413), TNF (MP6-XT22, Cat# 506329), IFN-γ (XMG1.2, Cat# 505846) were obtained from BioLegend. Anti-Foxp3 (FJK-16s ...
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bioRxiv - Immunology 2022Quote: The following antibodies were used to detect B cell populations in the BM: brilliant violet (BV) 421 anti-mouse CD19 antibody (Clone 6D5, BioLegend), PE/Cyanine5 anti-mouse/human CD45R/B220 (Clone RA3-6B2 ...
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bioRxiv - Immunology 2022Quote: ... The PBMCs were fixed and permeabilized using Foxp3 / Transcription factor kit (eBioscience) and stained intracellularly with anti-Granzyme-B-Alexa-Fluor-700 (Biolegend), anti-TNF-α-efluor-450 (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... and supernatants used for HLA-I IP in which purified anti-human HLA-A,B,C antibody (W6/32, BioLegend) was crosslinked to Protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: Activated and memory B cells were enriched from PBMCs by first staining with IgD-PE and MojoSort anti-PE Nanobeads (BioLegend), and then processing with the EasySep Human B Cell Isolation Kit using the EasyEights magnet (Stemcell ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Systems Biology 2024Quote: ... Ultra-LEAF anti-human HLA-A,B,C antibody (clone W6/32, cat#: 311448) for immunopeptidomics was purchased from BioLegend. Polyethylenimine (PEI ...
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bioRxiv - Molecular Biology 2020Quote: ... together with the trypsinized cells were harvested into tubes and samples were prepared on ice for flow cytometry using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend: 640926) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... from BD Sciences (San Jose, CA); Alexa Fluor® 647-conjugated anti-rat Thy1 (OX-7, #202508; RRID:AB_492884) from BioLegend (San Diego, CA). Ghost Dye™ Violet 450 was from Tonbo Biosciences (#13-0863-T100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples that were stained for cell surface staining only were incubated for 5 min on ice in 7-AAD (Biolegend, #420404). Samples that were stained for intracellular markers were fixed and permeabilized using the eBioscience™ FoxP3/Transcription Factor Staining Buffer Set (Thermofisher ...
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bioRxiv - Developmental Biology 2023Quote: The flow cytometry staining for necrosis and apoptosis was was performed with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, 640922). Once the cells were incubated in 10 µM of EdU ...
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bioRxiv - Cancer Biology 2024Quote: Apoptotic and necrotic cell analysis was performed using Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, #640926) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... 40592-V49H7-B) conjugated with PE-streptavidin or APC-streptavidin (TotalSeq-C0971 Streptavidin, BioLegend, 405271 and TotalSeq-C0972 Streptavidin, BioLegend, 405273), 0.0032 μg SARS-CoV-2 WT biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... YU2-gp140 fold-on, and hepatitis B surface antigen (HBs Ag) (Protein Specialists, hbs-875) conjugated to streptavidin Alexa Fluor 647 (Biolegend, 405237), streptavidin PE (BD biosciences ...
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bioRxiv - Immunology 2023Quote: ... and 7.5 nM of either a) tail-biotinylated cognate pMHC complex or b) biotinylated Armenian hamster anti-CD3ε antibody (clone 145-2C11, Biolegend 100304). ICAM-1 and pMHC proteins were expressed and purified as described (Wang et al. ...
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bioRxiv - Immunology 2023Quote: Cytokine levels in the supernatants from B cells that had been stimulated for 48 hr were measured using the LEGENDplex™ Human B cell Panel (13-plex) (Biolegend), using the same procedure as described for the antibody measurements ...
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bioRxiv - Immunology 2022Quote: Ten 10-week-old male wild type C57BL/6J mice were intravenously injected with an APC/Cyanine 7-conjugated anti-mouse CD45.2 antibody (Clone 104, BioLegend 109824, RRID: AB_830789) five minutes prior to euthanasia by CO2 inhalation ...
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bioRxiv - Cancer Biology 2021Quote: ... and early/late apoptosis was measured by using the PE Annexin V apoptosis detection kit with 7-AAD (Biolegend, San Diego, USA) according to the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... PerCP/Cy5.5-conjugated anti-mouse Ly6G mAb (Cat. #.: 127615) and 7-AAD Viability Staining Solution (Cat. #.: 420404) were purchased from BioLegend (CA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Then the tissues were washed multiple times in PBS at room temperature for one day and put into a staining solution containing Alexa Fluor 488 anti-mouse CD41 antibody (clone MWReg30, BioLegend) at a 1:100 dilution and Alexa Fluor 555 donkey anti-goat antibody (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescently-labeled antibodies directed against cell-specific surface marker for 30 min one ice in the dark (anti-mouse CD45-APC/Cy7, #103116, BioLegend; anti-mouse CD45-FITC ...
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bioRxiv - Immunology 2022Quote: ... Each of the three biological replicates that were treated with PBS vehicle control or TGM was labeled with one of three unique TotalSeq Hashtags Antibody markers (Biolegend). The samples were individually sort-purified by gating CD45+ cells and then each treatment group was pooled with an even number of cells from each sample ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
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bioRxiv - Cancer Biology 2023Quote: ... target tumor cells and effector NK cells were prepared as described above and one microgram of Anti-NKG2D (clone C7, BioLegend) or Armenian Hamster IgG isotype control (clone HTK888 ...
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bioRxiv - Immunology 2022Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.
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bioRxiv - Immunology 2020Quote: ... For investigating the formation of iBALT B cell and T cells were stained in lungs using primary antibody cocktail containing rat anti-mouse CD45R/B220 (BioLegend, Cat#103202) and Hamster anti mouse CD3 (BD ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell RNAseq data for the 7 PDA cell lines were generated as following: Cell lines were trypsinized and resuspended in Cell Staining Buffer (Biolegend, Cat. No. 420201) and incubated with proper Total Seq B antibodies using recommended protocol from Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were resuspended in 200 μL biding buffer with 2μL 7-AAD and 2μL annexin V-FITC (Biolegend Inc, San Diego, CA, USA). The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were expanded for one week and those that were double negative for MHC class I (Biolegend, W6/32, AF700) and β2 microglobulin (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: ... Multiplexing was performed by washing and hashing nuclei from each sample with one of six unique TotalSeq™-A anti-nuclear pore complex antibodies (BioLegend). Two multiplexed sequencing batches were then sequenced by the Oxford Genomics Centre (Wellcome Centre for Human Genetics ...
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bioRxiv - Bioengineering 2022Quote: ... fluorescence minus one (FMO) + isotype control antibody staining was performed using the following isotypes: PE/Cy7 Mouse IgG2a κ (cat # 400232, BioLegend), BV-421 Mouse IgG1 κ (cat # 400158 ...
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bioRxiv - Cell Biology 2022Quote: ... EGFR knockout cells were also validated by testing for loss of tyrosine phosphorylation in response to a one-minute treatment with 50 ng/mL mouse EGF by immunoblotting with mouse monoclonal antibody (PY20, BioLegend, 309301).
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bioRxiv - Immunology 2020Quote: Single live cells from individual mice were isolated by flow cytometry and labeled with TotalSeq-B cell hashing antibodies (Biolegend, San Diego, CA). Labeled cells were subjected to 3’ version 3.1 transcriptome library preparation using the Chromium 10X system (10X Genomics ...
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bioRxiv - Immunology 2020Quote: ... We divided the unstimulated cells per donor in half and stained one tube of unstimulated cells and one tube of stimulated cells per donor with an intracellular antibody master mix consisting of anti-IL17A – APC (BioLegend, Clone BL168) and PE conjugated to either anti-IL-4 ...
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bioRxiv - Immunology 2021Quote: ... Individually titrated working solutions containing one of eight hashtag antibodies were added to each of the eight respective patient samples (Biolegend; Table S13) and incubated on ice for 30 minutes followed by three magnet-based washes in FACS buffer ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated with primary antibody that recognizes an epitope within the humanized Aβ sequence using Purified Anti-B-Amyloid (BioLegend, Cat#803012, Lot#B251533) directly conjugated with horseradish peroxide (HRP ...
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bioRxiv - Immunology 2023Quote: ... prior to the end of the CD45- sort one vial of TotalSeq-C Human Universal Antibody Cocktail v1.0 (Biolegend, San Diego, CA, USA) was equilibrated to room temperature for 5min and then spun at 10,000g for 30 seconds ...
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bioRxiv - Immunology 2021Quote: Cryopreserved apheresis products were thawed and pre-labelled with antibodies for flow cytometry (described below) and a panel of TotalSeq™-B antibodies (described below) from Biolegend (San Diego, CA, USA). The panel of TotalSeq™-B antibodies included CD127 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained for one hour using the following antibodies: Allophycocyanin (APC) anti-human CD274 (5 μL/test; BioLegend #329707; BioLegend, San Diego, CA, USA), APC anti-mouse CD274 (5 μL/test ...
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bioRxiv - Cell Biology 2022Quote: ... - β1 (clone HMb1-1, 1:200) (Biolegend), were also employed ...
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bioRxiv - Neuroscience 2019Quote: ... 1-16 (1:1000, Biolegend). Sections were viewed ...
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bioRxiv - Cell Biology 2020Quote: ... Sca-1 (1:200; BioLegend), and Ter119 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cd49b-AF488 (Biolegend, clone HMα2, 1 µg ml−1, 1:500) and Sca1-AF647 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... flotillin-1 (W16108A; 1:1000; Biolegend), peIF2a (D9G8 ...