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Citations for BioLegend :
1201 - 1250 of 1273 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: The Fc receptors were blocked on 5X105 neutrophils resuspended in PBS with 5% FBS and TruStain FcX (1:50, BioLegend, 101320). Neutrophils were incubated with FITC conjugated anti-mouse Ly6G (1:50 ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... We then blocked the brains in 5 % goat serum for 2 h and stained with mouse anti-Aβ42 (6E10) (1:500, BioLegend, CA, USA) in PBST on a shaker at 4 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... for 20 minutes at 37°C and resuspended in cRPMI supplemented with 5 ng/mL recombinant mouse IL-2 (Biolegend #575404). 5×105 cells/well plated in a 96-well round-bottom plate ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (2,000 rpm 5 min) and then treated with 1 mL 1X red blood cell (RBC) Lysis Buffer (Biolegend; cat# NC9067514) to lyse red blood cells ...
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bioRxiv - Immunology 2024Quote: ... P14 T cells were stimulated with 100 ng/mL LCMV gp33 (KAVYNFATC) in the presence of 5 μg/mL Brefeldin A (BioLegend 420601) for 5 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... The fluorochrome-conjugated antibodies were as follows for flow cytometry: BV605 Rat Anti-Mouse CD4 (RM4-5/RM4-4; Biolegend #116027), PE-Cy™7 Rat Anti-Mouse CD8a (53-6.7 ...
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bioRxiv - Biochemistry 2024Quote: ... the brain sections were treated for 1 hour at room temperature with 5 μg/ml of an anti-human Aβ antibody (6E10, BioLegend, 803015). The cells were then seeded on coverslips at a density of 3 × 105 cells and cultured for 5 days in their respective media before being fixed with 4% paraformaldehyde/sucrose for 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and conditioned for 16h at 37 °C with cell-free AKPS-supernatant (1:5) or the following mouse cytokines at a concentration of 20ng/µl: TGF-β1 (BioLegend, 763102), IL-10 (PeproTech ...
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bioRxiv - Cancer Biology 2024Quote: ... or after a 4-day incubation period at 37°C with 5% CO2 (Day 4) following 30 min antibody staining and additional 15 min Annexin V APC binding (Biolegend, 640920). The antibodies used were as follow ...
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bioRxiv - Developmental Biology 2024Quote: ... The supernatant was removed after centrifugation at 500 g for 5 min and the cell pellet was resuspended in red blood cell lysis buffer (BioLegend, 420301) for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed twice with 2 mL of PBS + BSA (400 g for 5 min) and run fixed (Biolegend fixation buffer) or unfixed prior to sorting and analysis ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with 2 mL SB (350 g for 5 min) and subsequently with 2 mL of 1X Tandem Stabilizer (Biolegend #421802). Cells were resuspended in 200 µL of 1X Tandem Stabilizer.
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bioRxiv - Genomics 2020Quote: ... The cells were centrifuged at 300xg for 5 minutes at room temperature and resuspended in 85 ul of staining buffer and incubated with 5 ul of Human TruStain FcX(Biolegend cat: 422301) for 10 minutes on ice in 5 ml FACS tubes ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Following overnight incubation yeast were washed once in PBS/0.2% BSA and rotated at 18 rpm in same buffer containing 5 µg/mL mouse anti-His6 IgG (BioLegend, San Diego, CA) that had been conjugated with Alexa647 using the N-hydroxysuccinimidyl ester (Molecular Probes ...
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bioRxiv - Bioengineering 2021Quote: ... A 100 µL sample of EDTA-anticoagulated whole blood was incubated with 5 µL PE-Cy5 CD45 (HI30 clone, Biolegend, cat. # 304010) and 5 µL of PE-CD66B (Becton Dickinson ...
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bioRxiv - Biochemistry 2020Quote: ... Cells at a density of 2×107 cells/mL were incubated for 1 hour in flow cytometry buffer with 5 μL of Pacific Blue™ conjugated anti-CD235a antibody (BioLegend #349108) and 4 μg of unconjugated mouse IgG2a (Merck #M5409 ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 5×105 cells were stained for 30 min using the Zombie Aqua fixable viability dye (BioLegend Cat# 423101, 1:500 dilution), then fixed for 1 h in Foxp3/Transcription Factor Fixation/Permeabilisation Solution (ThermoFisher Cat# 00552300) ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Harvested lymphocytes were washed three times with FACS buffer (5% FBS in PBS) and stained for surface markers using: CD19-PE (363003, Biolegend, RRID: AB_2564125), IgD-FITC (348206 ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 75µL of Fc blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody, Biolegend in FACS buffer), and incubated for 20 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... About 5×106 bone marrow cells were plated into 100mm non-tissue culture treated plates containing 20ng/ml GMCSF (Biolegend, Cat# 640920). On day 3 5ml of fresh media containing 20ng/ml of GMCSF was added to the cultures ...
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bioRxiv - Microbiology 2022Quote: ... the remaining volume was subjected to immunoprecipitation on protein A/G magnetic beads coated with 5 µg of mouse anti-HA IgG (Biolegend, clone 16B12) as described in the Magna RIP technical manual ...
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... Blocking occurred using 5% BSA in TBST and antibody probing using 2% BSA in TBST at the indicated dilutions: α-pERK1/2 (Biolegend 369502) 5000x ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...
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bioRxiv - Cell Biology 2024Quote: ... Cells was washed with PBS and preincubated with 5 ml of TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Fc Receptor Blocking Solution, BioLegend, 156603) for 10 minutes on ice to reduce nonspecific immunofluorescent staining ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were centrifuged at 300×g for 5 min and washed with 10 mL cell staining buffer (#420201, Biolegend, San Diego, CA). Human FLRT2 antibody (#AF2877 ...
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bioRxiv - Bioengineering 2022Quote: ... 100 µl of 105/ml aAPC-BLMs containing all three ligands were stained with 5 µl of anti-mouse IgG PE (BioLegend, Cat#406607) and anti-human HLA-DR PE (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... CD4 (RM4-5), (anti-CD90.2 [30-H12], anti-CD44 [IM7], and anti-CD27 [LG-7F9]) (all from eBioscience/Invitrogen and Biolegend, San Diego, CA). Phenotype of polyclonal CD4+ T cell subsets and Plasmodium B5-specific CD4+ T cell subsets were identified with gating strategy as schematically depicted in S7 Fig ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were resuspended in 0.1% Tween-20 in PBS supplemented with 5% BSA at the following dilutions: TUJ1 (BioLegend, 801202, 1:400), SOX2 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... PerCP/Cy5.5-conjugated anti-mouse Ly6G mAb (Cat. #.: 127615) and 7-AAD Viability Staining Solution (Cat. #.: 420404) were purchased from BioLegend (CA, USA).
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies and channels were used to differentiate leukocyte populations CD4 BV510 (Biolegend Cat. No 100553, Clone RM4-5 0.038ng/µl), CD45 APC (Biolegend Cat ...
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bioRxiv - Immunology 2024Quote: ... in a 5% CO2 incubator at 37 °C for 7 days with 30 ng/ml IL-15 (BioLegend, San Diego, CA, USA) and 10 ng/ml IL-12 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were stimulated as a single-cell suspension at a concentration of 4-5 x 106 cells/mL with the Cell Activation Cocktail (Biolegend, Cat # 423301) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... and cell populations were analyzed by flow cytometry using the following antibodies: Brilliant Violet 510 anti-CD4 (clone RM4-5, BioLegend Cat# 100559), Brilliant Violet 785 anti-CD8a (clone 53-6.7 ...
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bioRxiv - Biochemistry 2024Quote: ... tumor samples from above were analyzed using the following extracellular antibodies: Brilliant Violet 510 anti-CD4 (clone RM4-5, BioLegend Cat# 100559), Brilliant Violet 785 anti-CD8a (clone 53-6.7 ...
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bioRxiv - Cancer Biology 2024Quote: ... RBC lysis was performed by resuspending the PBMCs in 5 ml of 1X RBC lysis buffer (RBC lysis buffer 10X; Biolegend cat# 420301) for 5 mins at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... supernatant was removed and nanovials were reconstituted at a ten-fold dilution in Washing buffer containing 5 μl of 100 μg/mL anti-IFN-γ BV421 (Biolegend, Catalog: 502532), 25 μg/mL anti-TNF-α APC (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... Nanovials were pelleted at the last wash step and incubated with 5 μl of 100 μg/mL anti-IFN-γ BV421 (Biolegend, Catalog: 502532), 25 μg/mL anti-TNF-α Alexa Fluor 647 (Biolegend ...
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bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells from healthy donors were activated in cell culture plates precoated with CD3 (5 μg/mL, BioLegend, San Diego, CA) and CD28 (1 μg/mL ...
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bioRxiv - Immunology 2022Quote: Primary human neutrophils isolated as described were stained at room temperature for 20 minutes with 5 μM Calcein AM (BioLegend, Cat. No. 425201) and applied to the top well of a fluorescence-blocking 24-well transwell plate with 3 μm pores (Corning ...
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bioRxiv - Immunology 2022Quote: ... Circulating maternal neutrophils were depleted at days 5 (E4.5) and 8 (E7.5) of pregnancy using a monoclonal neutralizing antibody (Biolegend, clone 1A8; 50 μg i.v.) or isotype control (Biolegend clone RTK2758 ...