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Citations for BioLegend :
851 - 900 of 1273 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were stained with 2.5ug of anti-CD4-APC (RM4-5, Biolegend 100516) antibody in 1mL of sort buffer and incubated at 4°C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... 5×105 SABR expressing NFAT-GFP-Jurkat cells were labeled with cell trace violet (BioLegend) according to the manufacturer’s protocol before incubation with 5×105 TCR expressing Jurkat cells in a round bottom 96-well plate for 16-20 hr ...
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bioRxiv - Immunology 2022Quote: ... Omicron RBD (BA.2 or BA.5) were labeled with TotalSeq-C0971 Streptavidin (Biolegend, 405271) and TotalSeq-C0972 Streptavidin (Biolegend ...
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bioRxiv - Physiology 2022Quote: ... and then stained for subsets of markers at 5 μg/ml: Ly6G-BV421(Biolegend, 127628), Ly6C-BV605 (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... spun for 5 minutes at 300 × g and washed once with 1x staining buffer (BioLegend). iNSCs were stained in suspension for 30 minutes at 37°C on an orbital shaker (500 rpm) ...
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bioRxiv - Immunology 2024Quote: ... mice were administered 5 µg Alexa Fluor (AF) 488-labelled anti-CD45.2 antibody (BioLegend, US) intravenously (i.v. ...
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bioRxiv - Cancer Biology 2024Quote: ... Then resuspend and count in 1640 complete medium containing CD28 (5 μg/ml, 302934, Biolegend) and IL-2 (10 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and matched isotype controls APC mouse IgG2b k isotype control (5 μL/test; BioLegend #400322), APC Rat IgG2b ...
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bioRxiv - Immunology 2023Quote: ... and cytokines (IFN-y and IL-5) were measured by ELISA (Biolegend. San Diego, CA). For intracellular cytokine determination ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were blocked using 5% goat serum and stained using antibodies against CD41 (133902, Biolegend) and laminin (L9393 ...
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bioRxiv - Immunology 2023Quote: ... Samples were then stained overnight at 4C with anti-CD4 (AlexaFluor488, clone RM4-5, BioLegend) and anti-CD45.1 (Alexafluor647 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 5 min at 4°C with biotinylated anti-CD3 antibody (17A2; BioLegend) to exclude CD3-positive T cells in the cell suspension using anti-biotin microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... we injected 5 μg of PE-Cy7 dye-conjugated anti-CD45 (Clone 30-F11, BioLegend) monoclonal antibodies (mAb ...
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bioRxiv - Cell Biology 2024Quote: ... human donor RGCs were pretreated with 5 mg/mL annexin V (BioLegend, San Diego, CA), a calcium-dependent membrane-bound protein with high affinity to PS ...
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bioRxiv - Immunology 2024Quote: ... Samples were then stained with 5 uL of Mojosort Human Dead Cell Removal Beads (BioLegend) for one round bead depletion ...
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bioRxiv - Immunology 2024Quote: ... and stimulated for 24 h with plate-bound anti-CD3 (5 μg/ml; 2C11; Biolegend) and anti-CD28 (2 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... and TotalSeq-C antibodies (0.078 μg per 5×106 PBMCs anti-human CD45RA (BioLegend, 304163), 0.277 μg per 5×106 PBMCs anti-human CCR7 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Five different TotalSeq-B antibodies were used in each experiment (Hashtag antibody 1-5, BioLegend). Incubation lasted for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... in diluted blocking buffer (1/5 in PBS) or its isotype control (Biolegend, Cat # 400132) at 4°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 min) and resuspended in 100 μl of serum medium supplemented with 0.5 μl SSEA-I (BioLegend, cat. no. 125607) and 0.15 μl PE-CD61 antibodies (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilization was followed with blocking step by incubating the samples for 2 hr in PBS containing 3% normal goat serum (NGS, 927503, BioLegend) and 3% BSA and then stained overnight at 4°C with the primary monoclonal antibody (mouse anti-radixin ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Slides were again washed 3 times with 1x TBS and stained with FITC–anti-IgD mAb (clone 11-26c.2a; 405713, BioLegend), PE goat-anti-mouse-IgM mAb (406507 ...
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bioRxiv - Immunology 2021Quote: ... Cells were stimulated for 3 hours at 37 °C with complete IMDM medium with 1x Cell Activation Cocktail (with Brefeldin A, Biolegend). After stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... BSA- and IgG-coated dynabeads for pre-clearing were prepared by mixing dynabeads slurry (40 μl per sample) with 500 μl PBS/3% BSA or 500 μl PBS with 2.5 μg mouse IgG1 (BioLegend, 400102) respectively ...
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bioRxiv - Immunology 2020Quote: ... Enriched cells were then stained for the indicated cell surface antigens (Supplementary Table 3) in conjunction with TotalSeq-A oligonucleotide-conjugated antibodies (BioLegend) to allow for barcoding and pooling of different TEC subpopulations and subsequently sorted into 4 subpopulations ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Developmental Biology 2020Quote: Cells were resuspended in 5ml CMF-PBS with 3% BSA (MilliporeSigma, A9576-50ml) and plated on anti-GD3 (Biolegend, 917701) 10cm plates (untreated plates coated overnight at 4°C with 13μg GD3 antibody in 10ml 50mM Tris-HCl pH9.5 ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... The MNC fraction was depleted of red blood cells by lysis steps involving 3 incubations at room temperature for 20 mins/10 mins/10 mins respectively with RBC lysis buffer (BioLegend). Tissue lymphocytes were isolated from Peyer’s patches in intestinal mucosa using laser capture microdissection and subjected to protein lysis as outlined above ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was assessed by flow cytometry after 3 days of drug or vehicle treatment using Helix NP NIR viability dye (BioLegend). Dose-response curves were fitted through interpolation using GraphPad Prism v.7 nonlinear regression fit ([inhibitor] versus normalized response–variable slope).
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... protein expression (ADT) and hashtag (HTO) libraries were generated using Chromium Single Cell 3’ version 2 reagents (10x genomics and Biolegend) per manufacturers’ protocols ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 50 μl FACS-buffer containing the respective fluorochrome-coupled Abs (anti-TACI/CD267 PE, clone eBio8F10-3, eBioscience, anti CD138 PECy7, clone 281-2, Biolegend, anti CD19 BV510 ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were pelleted and re-suspended in a staining solution of DMEM containing 1% FBS and anti-Ki-67 Alex Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour on ice ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...