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Citations for BioLegend :
1051 - 1100 of 1273 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognises residues 1-5 of human α-synuclein (7)] (BioLegend) at 1:4,000 ...
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Cancer Biology 2023Quote: ... BV650 anti-CD4 (clone RM4-5, 1:600), AlexaFluor700 anti-CD8 (clone 53-6.7, 1:800) were purchased from BioLegend. e506 Fixable Viability Dye (1:1000) ...
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bioRxiv - Immunology 2022Quote: Thawed 0.5×106 cells washed 3x at 400xg for 5 minutes at room temperature with 1x staining buffer (Biolegend) and kept on ice during the live dead staining using acridine orange and propidium dye to measure the cell viability before proceeding for 10x experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... The solution was filtered through a 40 μm filter and then placed in 5 mL RBC lysis buffer (BioLegend) for 5 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were dissolved in 5% BSA (Biofroxx, 4240GR005) and the dilutions were: Streptavidin-Cy3 (1:200, BioLegend, 405215), RL2 (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... an RBD - HC2.RBD.rFC (Metafora – 1:5) was used in conjunction with Alexa 647 donkey anti rabbit Poly4064 (1:50 Biolegend).
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bioRxiv - Immunology 2023Quote: ... 3x105 sorted naïve CD4 T cells were stimulated with plate-bound anti-CD3 (145-2C11, Cytek) (5 μg/ml) and soluble anti-CD28 (clone 37.51, Biolegend) (1 μg/ml ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting single cells suspension was used to detect leukocyte populations (CD45 BV421 [clone: 104; Biolegend], CD4 PerCPeFluor710 [RM4-5; eBioscience], CD8a APC-Cy7 [53-6.7; Biolegend], CD19 PE-Cy7 [6D5; Biolegend]) and CD4+ Foxp3+ Treg (CD45 BV421 ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were spun down and resuspended in 5 mL of 1x Red Blood Cell Lysis buffer (Biolegend) and incubated on ice for 5 minutes with occasional swirl ...
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bioRxiv - Cell Biology 2022Quote: ... poly-l-lysine coated glass slides were coated by overnight incubation with ICAM-1 (5 μg/mL, Biolegend 552906) or with ICAM-1 and anti-CD28 (5 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...
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bioRxiv - Bioengineering 2022Quote: ... 100 IU/mL recombinant human IL-2 (Proleukin, Prometheus Laboratories) and 5 ng/mL recombinant human IL-7 (BioLegend) at 37C ...
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bioRxiv - Cancer Biology 2023Quote: ... After 72 hours of incubation splenocytes were treated for 5 hours with activation cocktail with brefeldin A1 (Biolegend, 423303) before staining for CD45 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... in duplicate for 2hours at 37°C prior to adding CD16a.NK-92 cells (PTA-6967, ATCC) (5 × 104 cells/well) for 5hours with brefeldin A (Biolegend), Golgi Stop (BDBiosciences ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314, Biolegend) in sterile PBS at 37°C for 2 h ...
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bioRxiv - Immunology 2024Quote: ... in presence (stimulated condition) or absence (unstimulated condition) of 5 μg/ml anti-human CD3ε (clone OKT3; #317302, BioLegend) in PBS at 37°C for 2h ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and incubated with fluorescently labeled antibodies (CD4 PE, clone RM4-5, 1:400, BioLegend; CD8a FITC, clone 53-6.7, 1:400, BioLegend; B220 PE-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... Red blood cells were removed by incubating the cells with 5 ml ammonium-chloride-potassium (ACK) lysis buffer (Biolegend) for 5 min at room temperature followed by adding 10 ml RPMI supplemented with 3% FBS ...
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bioRxiv - Immunology 2024Quote: ... then resuspended in 200 µL of binding buffer and stained with 5 µL of 7-AAD (Cat#: 420403, Biolegend) for 15 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and individual hashtag antibodies (2.5 μL per 5×106 PBMCs of TotalSeq-C anti-human hashtag antibodies 1–8, BioLegend, 394661 ...
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bioRxiv - Immunology 2024Quote: ... individual hashtag antibodies (2.5 μL per 5×106 PBMCs of TotalSeq-C anti-human hashtag antibodies 1–8, BioLegend, 394661 ...
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bioRxiv - Cancer Biology 2024Quote: ... centrifuged at 500g for 5 minutes and resuspended in a solution of 1% bovine ser albumin (BSA) (Biolegend, 644710) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2024Quote: Bulk cultured T cells were stimulated with 5 µM peptides for 5hr in the presence of brefeldin A (Biolegend). The cells were collected and stained with specific antibodies against surface markers ...
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bioRxiv - Bioengineering 2024Quote: The T cells generated under standard conditions were stained on day 5 of culture with the antibodies Thy1.1-APC (Biolegend) and pan-TCR-α/β-PE (Beckman Coulter) ...
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bioRxiv - Bioengineering 2024Quote: ... anti-mouse IgG2a/b-FITC (5 g/ml) and anti-mouse CD138-PE (4 ug/ml; BioLegend cat# 142504); ii ...
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bioRxiv - Cancer Biology 2024Quote: ... 100k T-cells were mixed with 50k A375 or A375-STn cells (E:T ratio 1:1) in complete medium and 5 ug/ml brefeldin A (Biolegend). Co-cultures were incubated for 3.5 hours in a cell culture incubator ...
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bioRxiv - Biophysics 2021Quote: Flow channels were constructed as previously described but anti-TAMRA antibody was replaced with Anti-ß3 tubulin antibody (BioLegend Poly18020 anti-Tubulin Beta 3). Microtubule nucleation was achieved by introducing 5-50 μM tubulin (1:17 molar ratio of Atto-633 labelled:unlabelled ...
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bioRxiv - Neuroscience 2020Quote: The cerebellar slices were treated either 3 times 24 hours apart with combinations of recombinant TNF (recombinant rat TNF, Biolegend, San Diego, CA, USA), LTα (recombinant human LT-α ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
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bioRxiv - Cancer Biology 2023Quote: Other conjugated antibodies used in this study include the following: Alexa Fluor® 647 anti-Tubulin Beta 3 (1:1000, Biolegend, #657406, clone AA10), Alexa Fluor® 488 anti-Vimentin (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were stained at the same time with the APC conjugated surface marker antibody CD45 after a 5 min blocking step with Human TruStain FcX solution (20x, BioLegend) diluted in 1 % BSA/PBS ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed 3 times in TBST and incubated in TBST containing 5% non-fat dry milk and 1:1000 mouse anti-His (Biolegend) overnight ...
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated in PBS-5% FCS on ice for 30 min with rabbit anti-K10 (1:1000) (Biolegend), and K10 was visualized with anti-rabbit AF488 (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysates were treated with 8 ng/ml of RNase A and 2U/ml Turbo DNase for 5 min at 37° C and immunoprecipitated for two hours using anti-HA magnetic beads (901502, BioLegend). The beads were washed three times with lysis buffer and three times with PNK buffer (50mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... Ly6G-PE-Cy7+ and Ly6G- APC-) and intravascular PMNs (CD45-PerCP-Cy5·5+; Ly6G-PE-Cy7+ and Ly6G-APC+) (all antibodies from BioLegend) by flow cytometry (FACS Canto II ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel Parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Molecular Biology 2021Quote: ... while the MDR1 replicons were stained with 5 μL of PE anti-human CD243 (ABCB1) antibody clone 4E3.16 (Biolegend, US). After staining for 30 min on ice ...
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bioRxiv - Cell Biology 2022Quote: ... lung cells were brought to a single-cell suspension via collagenase incubation and then stained for flow cytometry at a concentration of 5×106 cells/mL with Cd31 (Biolegend) and 3μM ALOD4-47.
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bioRxiv - Cell Biology 2022Quote: ... cells were blocked with 5% bovine serum albumin (BSA) and 5% goat serum for 1hr at RT and incubated with primary antibodies (anti-βIII-tubulin antibody [BioLegend]) overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... at 0.5 μL/well for 5 hours and washed with PBS for detection of intracellular IFNγ using anti-hIFNγ APC antibody (Biolegend). The frequency of IFNγ positive cells was analyzed by flow cytometry (CytoFLEX II ...
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bioRxiv - Biochemistry 2022Quote: ... neutrophils (1×106 cells/mL) were added to each well in the presence of APC conjugated anti-CD11b antibody CBRM1/5 (BioLegend) at 1 μg/mL ...
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bioRxiv - Immunology 2020Quote: Cytokine concentrations in culture supernatant or mouse plasma were measured with the LegendPLEX Inflammation Panel (13-plex) or the Mouse Th1 Panel (5-plex) per the manufacturer protocols (BioLegend). Quantification was done using LegendPLEX software v8.0 for Windows ...
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25high Treg cells were enriched from PBMCs after staining with PE-cyanine 5 (PE-Cy5)-labeled anti-CD25 Ab (BioLegend), followed by positive selection using anti-PE and anti-Cy5 microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...