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Citations for BioLegend :
1151 - 1200 of 5275 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Cells were stained with CD19-PECy5 (BioLegend), then fixed and permeabilized with 1.6% paraformaldehyde and 90% ice-cold methanol ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and red cell lysis solution (Biolegend; #420301), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... and cells were dissociated with Accutase (Biolegend) and added to the supernatant ...
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bioRxiv - Immunology 2023Quote: ... and dead cell marker Zombie Violet (BioLegend). Percent of phagocytosis indicates the percentage of viable CD206+CFSE+ macrophages.
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bioRxiv - Microbiology 2023Quote: ... cells were incubated with Zombie NIR (Biolegend) for 5 minutes in PBS ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed with ICC fix (Biolegend) and stored at 4°C until intracellular staining was performed or cells were acquired.
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bioRxiv - Microbiology 2023Quote: ... detached using Accutase cell detachment solution (Biolegend) and seeded into 12 or 24 well plates (Corning) ...
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bioRxiv - Immunology 2023Quote: ... Cells were fixed with fixation buffer (Biolegend) for 15 minutes on ice ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were stained with Zombie Violet (Biolegend) or flexible viability dye eFluor 780 (eBioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were collected by accutase (Biolegend, 423201) and analyzed by flow cytometry for H-2Kb/H-2Db/H-2Dd (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Dead cells were excluded with Draq7 (Biolegend). Flow cytometric analysis was performed using the LSRFortessa (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with Brefeldin A (BioLegend) for 4h at 37 °C in 10% CO2 prior to staining ...
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bioRxiv - Cancer Biology 2023Quote: ... CD45+ (white blood cell marker [BioLegend: 103114]), PE+ (constitutively expressed RFP) ...
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bioRxiv - Microbiology 2023Quote: ... washed with Cell Staining Buffer (BioLegend 420201) and incubated with Fc block (purified anti-mouse CD16/CD32 antibody ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed with fixation buffer (Biolegend) and permeabilized with intracellular staining permeabilization wash buffer (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended with Zombie-dye (Biolegend) and incubated at RT for 15 min ...
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bioRxiv - Immunology 2023Quote: ... cells expressing CD31 (BV605, Biolegend, Cat. 303121) and CD34 (BV785 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-NIR (BioLegend) to discriminate live and dead cells ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded by DAPI (BioLegend) staining or LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with PI (Biolegend) to stain dead cells ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were fixed with Fixation Buffer (Biolegend). Cells were permeabilized using 0.3% saponin in FACS buffer (0.5% FBS in PBS) ...
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bioRxiv - Cell Biology 2023Quote: ... T cells (CD3, 17A2-APC, #100235, BioLegend), macrophages or granulocytes (CD14 ...
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bioRxiv - Cell Biology 2023Quote: ... washed with cell staining buffer (420201, BioLegend) and incubated with TruStain fcX™ (156604 ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with SiglecF-PE (Biolegend), CD19-APC (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... For live cell staining either DAPI (Biolegend) or Aqua fluorescent reactive dye (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... were mixed in Cell Staining Buffer (BioLegend), and applied to 100 μl of 2– 5×105 of single cells per reaction ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then resuspended Fc block (BioLegend) for 10 min at 4°C and stained with 1:200 anti-mouse antibodies (Abs ...
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bioRxiv - Immunology 2024Quote: ... a live cell dye (Zombie Aqua, Biolegend) was added to discriminate the dead cells ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with Zombie NIR (BioLegend) for 15 min ...
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bioRxiv - Systems Biology 2024Quote: ... the cells were mounted with DAPI (BioLegend) and images were acquired using the Celigo Image Cytometer (Nexcelom).
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bioRxiv - Cancer Biology 2021Quote: ... Cell lines or expanded primary NK cells were stimulated with 3 ug/mL CD28 activating antibody (Biolegend, cat#302933) for 24 hours.
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bioRxiv - Immunology 2022Quote: ... Stimulation of naïve CD4+ T cells was confirmed by their increased cell sizes and expression of the activation marker CD44 (BioLegend) by FACS analysis ...
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bioRxiv - Microbiology 2022Quote: ... total lymphocytes were counted and naïve B cells were isolated using Mojosort Naïve B cell isolation beads (Biolegend 480068) or Naïve B cell Isolation Kit II (Miltenyi 130-091-150 ...
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bioRxiv - Immunology 2019Quote: ... After incubation cell suspensions were filtered through 70-μm cell strainer and stained with CD45-PerCP (30-F11; Biolegend), CD11b-PacBlue (M1/70 ...
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bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
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bioRxiv - Neuroscience 2020Quote: ... Then we resuspended the pelleted cells in 200μl Fluorescent Activated Cell Sorter (FACS) buffer [see Homogenization Buffer ingredients but dissolved in Cell Staining Buffer (Biolegend) not aCSF ...
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bioRxiv - Cell Biology 2021Quote: ... CD4+ T cells were then isolated by negative selection using the MojoSort Mouse CD4 T Cell Isolation Kit (Biolegend). Isolated CD4+ T cells were seeded at 1×106/mL in RPMI supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with 100 μl/well of the fixable dead cell viability dye Zombie Aqua (1:800, Biolegend) diluted in PBS for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... passed through a 100-µm cell strainer and red blood cells were lysed with the RBC lysis buffer (Biolegend). To differentiate between live and dead cells ...
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bioRxiv - Immunology 2022Quote: Cells were washed once with PBS and dead cells were stained with Zombie Green fixable viability dye (Biolegend, 423111), 1:10.000 in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Cells overexpressing Rab35 were detected using an anti-HA primary antibody (data not shown; Rabbit, Cell Signaling; Mouse, Biolegend) + Alexa Fluor-405 secondary antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... the cell suspension was pelleted by centrifugation and cells were resuspended in FACS buffer containing 7AAD (BioLegend, 1:100). The flow cytometry analysis was performed on an LSR-II flow cytometer (BD Biosciences) ...
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bioRxiv - Bioengineering 2019Quote: ... and CD31+ cells were isolated using flow cytometry sorting after staining cells with anti-mouse CD31 antibody (Biolegend 102417) and plated onto Vitronectin-coated plates (1mL/well at 1µg/cm2 ...
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bioRxiv - Physiology 2019Quote: CD4+CD25+ cells were isolated using negative selection of CD4+ T cells using the MojoSort Mouse CD4 Naïve T-cell Isolation Kit (catalog #480040, Biolegend) followed by positive selection of CD25+ ...
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bioRxiv - Genomics 2021Quote: ... Isolated 2×106 PBMC cells were incubated with PMA/ionomycin (i.e., stimulated) (Cell Activation Cocktail with Brefeldin A, Biolegend), or DMSO as control (i.e. ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-PE Cy7, 1:200 Biolegend, # 101216 ...
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bioRxiv - Cancer Biology 2020Quote: ... a maximum of 2 million cells per sample was resuspended in 100 µl of cell staining buffer (Biolegend, USA) with 5 µl of Fc receptor Block (TrueStain FcX ...
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bioRxiv - Immunology 2021Quote: ... Cells from this single cell suspension were washed with DPBS and stained with Zombie NIR fixable viability dye (BioLegend) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4 T cells (106 cells/mL) were activated in vitro using a combination of plate bound anti-CD3 (2μg/mL) (BioLegend) and soluble anti-CD28 (2μg/mL ...
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bioRxiv - Immunology 2020Quote: CD8 T cells were isolated from an OT1+ mouse using the mojosort mouse CD8 T cell isolation kit (Biolegend) and labeled with violet proliferation dye (BD Biosciences cat# 562158) ...