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Citations for BioLegend :
1051 - 1100 of 5275 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated using the MojoSort Human CD3 T Cell Isolation Kit (cat. no. 480021, Biolegend) and EasySep Magnet (cat ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 2 times in 1x Perm/Wash buffer and resuspended in Cell Staining Buffer (Biolegend) prior to flow cytometry analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... and the cells were counted and resuspended in 1 ml of cell staining buffer (CSB) (BioLegend, 420201) for each sample ...
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bioRxiv - Immunology 2024Quote: ... T cell activation was assessed by incubating cells with fluorescein isothiocyanate-conjugated anti-human CD8 (Biolegend, 100705) and phycoerythrin-conjugated anti-human CD25 (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T Cells were isolated using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035) and resuspended in RPMI-1640 medium (Corning ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were enriched using a MojoSort™ T cell isolation kit (BioLegend, Cat no. 480006) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... one million cells were harvested 3 days after transduction and washed twice with cell staining buffer (Biolegend). Cells were resuspended in 100μL of Cell Staining Buffer (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: Cell death was determined using flow cytometry through the quantification of cell surface annexin V-APC (Biolegend) and propidium iodide (PI ...
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bioRxiv - Genomics 2022Quote: ... cells were activated at ∼2-4M cells/ml for 8 hrs using CD3 antibody (Biolegend clone OKT3) coated tissue culture plates ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from the spleen using MojoSort Mouse CD4 T cell isolation kit (BioLegend) and sorted according to their expression of CD44 and NKG2D ...
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bioRxiv - Immunology 2023Quote: ... B cells and monocytes were isolated from PBMCs (AllCells) using Mojosort magnetic cell separation system from Biolegend via CD3 positivity (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells were obtained from peripheral blood after lysis of red blood cells (Biolegend, 420301). Flushing femurs and mechanical disruption of inguinal lymph nodes were performed to obtain cells from bone marrow and lymph nodes.
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bioRxiv - Cancer Biology 2024Quote: ... a single-cell solution of lung cells was incubated with Human TruStain FcX (Biolegend, #422302, 1:20) for 10 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... cells were stained with live dead cell staining dye NIR and then incubated with Fc block (BioLegend). Cells were further stained with the following fluorochrome-conjugated antibodies ...
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bioRxiv - Immunology 2024Quote: ... splenic B cells were isolated according to the MojoSort™ Mouse Pan B Cell Isolation Kit (BioLegend) protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Splenocytes and BM cells were depleted of red blood cells with RBC lysis buffer (Sigma or BioLegend). Cell suspensions were incubated with mouse Fc Block (BD or BioLegend) ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from bone marrow chimeras (MojoSort™ Mouse Pan B Cell Isolation Kit; Biolegend). Control and WNK1-deficient B cells were labelled with CellTracker blue (CMAC ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in ice-cold PBS with 1:500 dilution of Zombi-NIR dead cell stain (Biolegend) and incubated for 15 minutes in the dark on ice ...
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bioRxiv - Neuroscience 2019Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-BV650, 1:200 Biolegend, #141723 ...
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bioRxiv - Bioengineering 2020Quote: ... The non-macrophages cell fraction was then incubated in mouse CD4+ T cell isolation biotin-antiboy cocktail (BioLegend) and sorted ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with antibodies for cell surface markers in FWB plus fixable viability dye (Zombie Red; BioLegend), then fixed and permeabilized using the BrdU APC kit (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Neuroscience 2022Quote: ... Dead cells were excluded by labelling with the cell viability dye Zombie Aqua (2 ng/ml, BioLegend, 423101) that was added to the single cell suspension before sorting ...
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bioRxiv - Immunology 2022Quote: ... Proliferation was determined by labeling T cells with the Tag-IT Violet Proliferation Cell Tracking Dye (Biolegend, #425101), according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2019Quote: Cells were detached using 2mM EDTA in PBS and cell surface expression of CD49b was measured (Biolegend 359310). Annexin V staining was performed using FITC Annexin V apoptosis Detection Kit (BD Biosciences 556547).
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bioRxiv - Immunology 2019Quote: ... Cell surface phenotyping of transduced cells was determined by staining with BV785-conjugated anti-human CD3 (UCHT1, BioLegend), BV605-conjugated anti-human CD4 (OKT-4 ...
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bioRxiv - Biochemistry 2019Quote: ... cells were lysed and the detergent-solubilized cell lysates were incubated with an anti-HA antibody (BioLegend, 901503) at 4 °C overnight ...
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bioRxiv - Immunology 2020Quote: For mouse lung cell staining: cell suspensions were incubated with purified anti-mouse CD16/32 (clone 93, BioLegend) for 10 minutes at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained with the appropriate surface markers [dead cell marker-Zombie Aqua™ Fixable Viability Kit (Biolegend)] and CD8 (APC conjugated ...
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bioRxiv - Microbiology 2020Quote: ... the GFP+ cells were quantified by flow cytometry with pacific blue viability dye to exclude dead cells (Biolegend).
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bioRxiv - Immunology 2021Quote: ... and CD3+ cells were depleted from the donor cells using a biotinylated anti-mouse CD3e mAb (BioLegend; 100304) and the Miltenyi MACS® MicroBead (Miltenyi ...
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bioRxiv - Immunology 2021Quote: ... Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Immunology 2020Quote: ... Cells were placed in V-bottom plates and stimulated with 500X Cell Activation Cocktail with Brefeldin A (Biolegend) in IMDM medium supplemented with 8% FCS ...
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bioRxiv - Immunology 2021Quote: ... CD3+ cell enrichment was achieved by MojoSort negative selection using MojoSort mouse CD3+ T-cell Isolation Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells (naïve or total) were enriched with naïve or total CD4+ T cell negative enrichment kits (Stemcell, Biolegend) from the spleens of female ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4 T cells were presorted with the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend) based on provided directions ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with fluorophore-labelled antibodies targeting the following cell surface markers: APC-SiglecH (Clone 551, BioLegend), APC-Cy7-CD11b (Clone M1/70 ...
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bioRxiv - Immunology 2024Quote: ... single-cell suspensions were stimulated with cell activation cocktail (including phorbol 12-myristate 13-acetate and Ionomycin) (BioLegend) in the presence of Golgi Stop (monensin ...
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bioRxiv - Cell Biology 2023Quote: ... and T cells were isolated from the spleen using the MojoSortTM Mouse CD3 T cell Isolation Kit (Biolegend). Experiments where purified T cells were cultured for longer than 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Cell Biology 2022Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ T cell isolation kit (BioLegend) and cultivated as described above ...
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bioRxiv - Genomics 2023Quote: ... 500,000 cells from bulk and CD45+ enriched fraction were individually re-suspended in Cell Staining Buffer (420201, Biolegend) and blocked with 5 µL Human TruStain FcX™ (422301 ...
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed by centrifugation (250g for 10 min) with FACS cells staining buffer (Cat# 420201, BioLegend). Cells were next treated with human 5µl/tube TruStain FcX™ (Fc receptor blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: ... transgenic T cells were labeled with Tag-it Violet Proliferation and Cell Tracking Dye (Cat No. 425101 Biolegend). The frequency of SMARTA cells that proliferated during culture was determined as described by Gett et al ...
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bioRxiv - Cancer Biology 2023Quote: ... harvested cells were incubated in Roswell Park Memorial Institute medium with cell activation cocktail with brefeldin A (Biolegend) for 4.5 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: Naïve B cells were isolated from spleens by immunomagnetic depletion using MojoSort Pan B cell Isolation Kit (Biolegend) and cultured in RPMI supplemented with L-glutamine ...