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Citations for BioLegend :
1401 - 1450 of 5275 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The cells were stained with pSTAT5 BV421 (BioLegend) antibody and analysed by flow cytometry ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability dye DRAQ (BioLegend, California, USA; # 424101) was added to the medium at room temperature for 30 minutes prior to live-cell imaging.
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bioRxiv - Cancer Biology 2023Quote: ... cells were directly stained with anti-CD45 (BioLegend) and viability stained with PI and Hoechst ...
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bioRxiv - Cell Biology 2023Quote: ... counted and resuspended in Cell Staining Buffer (BioLegend). Cells were counted using a Countess II™ (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with 7-AAD (BioLegend, #420404) cellular DNA content marker (5 μl per sample ...
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bioRxiv - Immunology 2024Quote: ... lung cells were detached by Accutase (Biolegend, USA) and the endothelial cells were separated using Dynabeads coupled to anti-CD31 antibody and anti-ICAM2 antibody (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were blocked with Human TruStain FcX (BioLegend) and stained using the listed antibodies (Supplemental Table 1) ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were dyed with DRAQ5 (BioLegend, 424101) immediately at the end of each experiment and imaged on an Incucyte SX5 live cell imaging and analysis system ...
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bioRxiv - Immunology 2024Quote: ... A dead live-cell marker (Zombie Aqua, BioLegend) was also included ...
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bioRxiv - Immunology 2024Quote: ... red blood cells were removed by lysis (BioLegend) and filtered through a 70 µm filter ...
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bioRxiv - Immunology 2024Quote: ... NK cells were distinguished using anti-CD16 (Biolegend) and anti-CD56 (BD Horizon) ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with anti-CD56 (5.1H11, Biolegend) and anti-CD16 (3G8 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cell death marker Zombie Yellow (BioLegend, # 423103) was added to the culture before imaging ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated with antibodies - CD45-APC (Biolegend), Ly6G-FITC (eBioscience) ...
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bioRxiv - Developmental Biology 2021Quote: ... Final volumes of 500 μl resuspended cells were placed on ice and sorted by fluorescence activated cell sorting (FACS) to isolate live cells that excluded the cytoplasmic stain Zombie green (BioLegend, 423111). For scRNAseq library construction ...
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bioRxiv - Immunology 2020Quote: ... Blood CD4+ T cells were isolated from healthy donor or patient PBMCs using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were selected using hygromycin and the stable population was sorted on our single cell platform for CD19 expression levels by staining transfected cells with AlexaFluor® 647 anti-CD19 antibody (#302222, Biolegend). Cells were sorted for different expression levels of CD19 and clonal cell lines with expression levels comparable to that of standard CD19 positive cell lines such as Raji ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... CD81 expression was measured 7 days post-transduction by dislodging cells with TrypLE and live cells were stained with APC-conjugated anti-human CD81 antibody (Biolegend 349509). CD81 expression was assessed on a BD LSRII with >90% of Calu3-CRISPRi cells with CD81 knocked down compared to a non-targeting sgRNA control.
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bioRxiv - Cancer Biology 2020Quote: ... followed by sorting of GFP- cells and magnetic negative selection-were incubated with Cell Hashing HTO-conjugated antibodies (Biolegend, TotalSeq(tm) anti-mouse Hashtag reagents Cat# A0301-A0306 ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mice were used whose cells were labelled with Hashtags oligos (HTO) using the Cell Hashing protocol from Biolegend (TotalSeq-A). All the sorted cells were pooled on the same tube and processed in the Chromium Next GEM Chip G using Chromium Next GEM Single Cell 3’ v3.1.
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from the resulted mononuclear cells using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). Single molecule FISH experiments were carried out using an RNAscope® Multiplex Fluorescent Assay v2 along with an RNAscope® Probe-Mm-Krt14-C2 (ACD cat ...
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bioRxiv - Microbiology 2020Quote: ... Splenic cell suspensions were treated additionally with red blood cell lysis buffer at room temperature for 10 min (Sigma-Aldrichor BioLegend Inc) for removing RBCs to obtain single cell suspensions ...
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bioRxiv - Immunology 2022Quote: ... with the following modifications: Approximately 1 million cells per sample were resuspended in 95 ul of cell staining buffer (Biolegend, USA) with 5 ul of Fc receptor Block (TrueStain FcX ...
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bioRxiv - Cancer Biology 2019Quote: ... Purity of isolated cells was validated by FACS by using the cell surface markers EpCAM-APC (Biolegend, 118213, 1:50 dilution) and CD45-PE (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells from blood were isolated from healthy donor peripheral blood mononuclear cells (PBMCs) using the BioLegend human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333 ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and naïve CD8+ cells were obtained using the MojoSort Human CD8+ Naïve T Cell Isolation Kit (Biolegend, Cat no.480045). Purity was consistently over >75% CD8+ with no contamination of CD4+ T cells and >80% of CD8+ T cells being naive ...
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bioRxiv - Genetics 2021Quote: ... Cells were then washed twice with cold PBSx1 and the cells were incubated with live/dead Zombie Violet Dye (Biolegend, 423114) for 15 min at RT as suggested by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... cells were initially blocked with anti-mouse Fcγ III/II receptor (CD16/CD32) antibodies and dead cells excluded by staining with a fixable live/dead cell-exclusion dye (BioLegend, USA). Then ...
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bioRxiv - Immunology 2022Quote: ... Non-adherent cells were removed after 30min and adherent cells were incubated with αMHC DP-DQ-DR mAb (BioLegend, clone Tü39) or isotype control (IgG2a ...
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bioRxiv - Microbiology 2022Quote: The amount of TNFα secreted in the supernatants from cell culture and cell lysate was determined by ELISA MAX™ Deluxe Set Mouse TNFα kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CORSET8 T cells were purified from the TCR Tg donor mice with the MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2024Quote: ... Lymph node cells were harvested from TCR Tg donor mice and CD8+ T cells were purified with MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... HTO stained PBMCs from all 10 donors were then pooled and stained with oligo-tagged TotalSeq C antibody for cell surface proteins for T cell memory markers (CD45RA, CCR7, CD95 and IL7Rα) (0.5 µg/condition; Biolegend, Table S2B) for 30 minutes on ice ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany]; CD31+ endothelial cells: 1:2000 anti-CD31-PE [BioLegend, CA]/1:50 anti-CD45-VioGreen [Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: ... PDX tumors were digested to a single cell suspension in Collagenase digest buffer and sorted for human cells using anti human HLA-ABC (Biolegend, 555555), DNA and RNA were extracted using an AllPrep Kit (Qiagen ...
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bioRxiv - Immunology 2023Quote: Cytokine levels in the supernatants from B cells that had been stimulated for 48 hr were measured using the LEGENDplex™ Human B cell Panel (13-plex) (Biolegend), using the same procedure as described for the antibody measurements ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pellets were harvested and resuspended in 5 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 5 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... FoxP3-transduced CD4+ T cells were stained with antibodies targeting the cell surface antigens CD25 (Biolegend, Cat#102022, 1:1000 dilution) and Thy1.1 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... For adoptive transfer and co-culture experiments naïve OT-II cells were magnetically enriched with MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend Cat: 480040) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... to assess viable cells and fluorophore-conjugated monoclonal antibodies to the following human cell-surface markers: anti-CD45-BV650 (Biolegend, 304044), anti-CD206-AF647 (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... CD2 target gene activation was quantified by cell surface antibody staining of live cells using APC anti-human CD2 antibody (Biolegend, 309224) followed by flow cytometry on the cell population transfected with both gRNA and dCasMINI plasmids (performed on Cytoflex and analyzed using Flowjo software) ...
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bioRxiv - Neuroscience 2024Quote: At passages 11-13 pericytes at 90-95% confluence from T-75 cell culture flasks were dissociated with Accutase® Cell Solution (BioLegend) and prepared for flow cytometry to analyze subtype distribution in vitro ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then stained with the following conjugated cell-surface marker antibodies at 1:50 dilutions: CD326 (EpCAM) PE (Biolegend, 324206) and CD45 BV786 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated by negative selection using the EasySep Human B Cell Isolation Kit (StemCell, #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, # 480082) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... Naive CD4+ cells were isolated by negative selection using the MojoSort CD4+ naïve T-cell isolation kit from Biolegend (Catalog 480040) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... five groups of cells were pre-labelled with mouse HashTag oligos (HTO) on cell surface: spleen T cell control group (SPT ctrl) with hashtag 1 (BioLegend, Cat# 155801); spleen T cell IL-1β/IL-23 stimulation group (SPT stim ...
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bioRxiv - Immunology 2019Quote: ... human T cells were directly enriched from PBMC by negative selection using MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, CA) following the manufacturer’s instructions ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: In order to separate cells of different origin we further stained the cell suspension with antibodies against CD45 (Biolegend #103107; 1:200), FAP (Abcam #ab28244 ...