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Citations for BioLegend :
1151 - 1200 of 1839 citations for 6 HYDROXY 2 METHYL PYRIMIDINE 4 ACETIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... and IL-2 or IFNγ were measured by ELISA following the manufacturer’s protocol (BioLegend). Absorbances were measured with a Synergy H1 Hybrid Multi-Mode Reader (BioTek).
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bioRxiv - Immunology 2024Quote: IL-2 and IFN-γ levels were measured with a sandwich ELISA kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were incubated with 4,6-diamidino-2-phenylindole (DAPI) and fluorophore-conjugated antibodies (BioLegend) against CD31 ...
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bioRxiv - Immunology 2023Quote: ... Cells were isolated from blood by adding 2 mL of RBC lysis buffer (Biolegend) per 100 uL of blood collected ...
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bioRxiv - Immunology 2024Quote: ... IFNg-PeCy7 (cat # 505826) and IL-2-PB (cat # 503820) were purchased from Biolegend. CD44-Percp-cyanine5.5 (cat# 45-0441-80 ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µL (1 µg) of the corresponding hashing antibody (Totalseq-A, Biolegend, Table S4) were added to the blocked samples ...
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bioRxiv - Genomics 2024Quote: ... antibody for Th1 conditions or 10ng/mL rhIL-2 and 5ng/mL rhTGFý (Biolegend) for Treg conditions.
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/mL of anti-IgE (clone RME-1, BioLegend) and for IgG1 ELISAs ...
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bioRxiv - Immunology 2024Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...
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bioRxiv - Immunology 2024Quote: ... with 3 µg of APC-labeled anti-CD8b.2 antibody (clone 53-5.8, Biolegend) 5 min prior to euthanasia to mark vasculature-associated cells in vivo.
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bioRxiv - Immunology 2023Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... BV421 rat anti-mouse CD45 (cat # 103133, BioLegend, clone 30-F11, 2 ng/μl), PE rat anti-mouse CD11b (cat # 101208 ...
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bioRxiv - Neuroscience 2023Quote: ... PE rat anti-mouse CD11b (cat # 101208, BioLegend, clone M1/70, 2 ng/μl), APC rat anti-mouse ACSA-2 (cat # 130-117-535 ...
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bioRxiv - Immunology 2023Quote: ... and secretion of IFNγ and IL-2 were measured by ELISA (ELISA MAX, BioLegend) in supernatant removed after 24-hour incubation.
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bioRxiv - Cell Biology 2022Quote: ... Phycoerythrin-conjugated anti-human tetherin (BioLegend, RS38E, FC 2 μg / 100 μl / 106 cells), Pacific Blue-conjugated anti-CD71 (Exbio ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1-2 million cells were incubated in 45ul of Cell Staining Buffer (BioLegend) per million cells with Trustain FcX block (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... biotinylated SARS-CoV-2 Spike RBD protein was mixed with Streptavidin-BV421 (405225, BioLegend) at a 4:1 molar ratio for 1 hour at 4 °C to obtain the antigen probe ...
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bioRxiv - Immunology 2023Quote: ... H-2Dd (Anti-mouse H-2Dd antibody; clone 34-2-12; Biolegend; Cat #110606), CD25 (BV510 Rat Anti-Mouse CD25 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used for studies related to the Fra-2 model were purchased from Biolegend. Assessment of fibrosing alveolitis by Micro-CT images were obtained with a Perkin Elmer Quantum FX system (Caliper Life Sciences ...
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bioRxiv - Cancer Biology 2024Quote: Experiment 2: All conditions were multiplexed using Hashtag Oligonucleotide barcoding technology (TotalSeq-B, Biolegend) following manufacturer standard protocol ...
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bioRxiv - Immunology 2024Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with Zombie NIR (Biolegend ...
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bioRxiv - Physiology 2024Quote: ... with 2% with Fetal Bovine serum with Fc block (Biolegend, San Diego, California, USA) and stained with monoclonal antibodies specific for CD66b (clone GM2H6) ...
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bioRxiv - Cell Biology 2019Quote: ... Fixed and blocked hMPCs were incubated overnight at 4°C with an anti-BrdU antibody (1:400 dilution, Biolegend) followed by PBS washes and a 60 min incubation with an Alexa-Fluor 488 conjugated anti-mouse secondary antibody (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse interleukin (IL)-4 PE (504104) and anti-mouse IL-17 PE (517008) (BioLegend, San Diego, CA, USA) were used ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were collected from culture dishes and incubated for 30 min at 4 °C with CITE-seq cell hashing62 antibody Ab_CD15 (1:200) (Biolegend). XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200) (Biolegend). In the gastruloid sample ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated overnight at 4°C with anti-Aβ primary antibody (6E10 Biolegend, SIG-39320, 1:1000) following incubation with goat anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... The resuspended cells were incubated for 20 min at 4 °C with the fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 4°C for 15 minutes with an Fc receptor blocker (BioLegend TruStain FcX, cat # 422302) according to manufacturer instructions before cocktails of surface antibodies were added for 30 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Samples were next stained for surface markers for 20min at 4°C with: PerCP-Cy5.5-conjugated anti-TCRβ (H57–597, BioLegend), APC-CY7-conjugated anti-B220 (RA3-6B2 ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated overnight at 4°C with mouse anti-HA primary antibody (1:1000, Anti-HA.11, Biolegend), washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Immunology 2019Quote: ... Depletion was confirmed by analysis of dLN and skin T cells stained with anti-CD4 clone RM4-4 (Biolegend), which binds a nonoverlapping epitope.
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bioRxiv - Biochemistry 2020Quote: ... cells were cultured overnight with or without 4 μg/mL Syrian hamster anti-mouse CD28 (clone 37.51, BioLegend, USA) and plate-bound Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBS-Tx (1:1,000 for mouse anti-HA, #901514 BioLegend; 1:1,000 for rabbit anti-TOM20 ...
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bioRxiv - Cell Biology 2020Quote: After coating with poly-L-Lysine, fluorodishes were coated overnight at 4 °C with αCD3ε (OKT3, #16-0037-85, eBioscience) + αCD28 (CD28.2, #302914 Biolegend), washed three times and pre-warmed at 37 °C for 10–15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2022Quote: ... and stained for 30 min at 4°C with a fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD4 Ab (BioLegend). The cells were then fixed ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Immunology 2020Quote: ... then stained 15 minutes for surface markers and fixed for 20 minutes with 4% paraformaldehyde (PFA) in PBS (BioLegend). Cells were then permeabilized with 0.5 % saponin in Running buffer and stained for intracellular markers ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 μL of WCE was incubated overnight at 4°C with 10μg of monoclonal RNA Poll antibody (Biolegend, USA), 2μg of anti-myc antibody (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tissue was then blocked with 10% normal donkey serum/0.1% Triton X- 100/0.01% NaN3 followed by staining with Alexa Fluor 488 conjugated anti-human CD4 OKT 4) antibody (Biolegend) at 4° Celsius overnight (1:200 diluted in PBS) ...
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bioRxiv - Microbiology 2021Quote: ... Primary CD4+ T cells were activated for 4-5 days on T25 flasks coated with 5μg anti-CD3 antibody (clone OKT3, Biolegend) in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2 ...
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Immunology 2020Quote: ... Sections were stained at 4°C on an agitation stage overnight with anti-CD169 and anti-Ly6G antibodies (BioLegend). Stained sections were microscopically analyzed using Leica SP8 confocal microscope (Leica Microsystem ...
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bioRxiv - Cancer Biology 2022Quote: ... we collected the beads and labeled them with 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6) for 45 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed in PBS and were incubated at 4°C with CD16/32 Fc block (cat#101302, BioLegend) in PBS for 10 minutes before staining with antibodies (Supplementary Table 1 ...