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Citations for BioLegend :
1251 - 1300 of 1839 citations for 6 HYDROXY 2 METHYL PYRIMIDINE 4 ACETIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 2×106 single splenocytes or BAL cells were incubated for 4 hours at 37° C in the presence of PMA/Ionomycin and Brefeldin A (Cell Activation Cocktail; BioLegend) or GolgiPlug control (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
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bioRxiv - Neuroscience 2020Quote: ... samples have been incubated overnight at 4°C with primary antibody anti β-amyloid (Aβ) (clone 6E10, 1:500, BioLegend) diluted in blocking buffer and stained with the corresponding goat secondary antibody ...
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bioRxiv - Genetics 2020Quote: ... and stained at 4°C in a final volume of 50µL with the following antibodies: CD14-FITC (1:50)(Biolegend 301803), CD16-PE (1:50)(Biolegend 3G8) ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend] ...
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bioRxiv - Immunology 2022Quote: ... or DC in vitro culture (5×105) were washed with PBS at 400 g for 5 min at 4°C and blocked with FcX (BioLegend) for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-P62 (SQSTM1) (1:500, #814802, BioLegend), anti-CD107b (LAMP-2 ...
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bioRxiv - Microbiology 2022Quote: ... and then stained for 30 minutes at 4℃ with the following antibody panel each diluted in PBS: APC/Cyanine7 anti-mouse CD45 antibody (Biolegend) or FITC anti-mouse CD45.2 antibody (Biolegend) ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were incubated with cell surface markers for one hour at 4 °C and then washed with PBS before being resuspended with Annexin V binding buffer (Biolegend). Cells were then incubated with FITC Annexin V (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... fixed with 4% PFA for 15 minutes and subsequently stained for 30 minutes with anti-CD4-APC and anti-CD8-BV605 (Biolegend) in FACS buffer ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Cell-free BAL fluid was stored at 4°C and processed within 24hr to measure cytokine/chemokine content using a LEGENDplex bead array kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... 2 × 105 WT or CD56-KO NK92 and YTS cell lines were added to 12-well flat-bottomed plates that were pre-coated overnight at 4°C with anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Neuroscience 2021Quote: ... and finally incubated overnight at 4°C with primary antibodies against NKCC1 (T4, 1:1000, DSHB) and tubulin (anti-Tuj1, 1:5000, Biolegend) diluted in the blocking buffer with 0.1% tween ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318; anti-CD31 APC/Fire 750, Biolegend 102433 ...
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bioRxiv - Microbiology 2021Quote: ... and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies: from Biolegend: PacificBlue-conjugated anti-Ly-6C (clone HK1.4 ...
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bioRxiv - Immunology 2019Quote: Macrophages were stained for 40 min at 4°C with fluorophore-conjugated mAb FITC-anti-CD36 (clone 5-271, Biolegend), or PE-anti-CD209 (clone 120507 ...
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bioRxiv - Immunology 2021Quote: ... Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4) and an anti-PE oligonucleotide barcode (BioLegend). Cells from each compartment (i.e ...
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bioRxiv - Microbiology 2021Quote: T cells were washed and incubated with antibodies (described above) at 4°C for surface staining and cell viability dye Zombie UV (Biolegend). Primary antibodies were detected with anti-human IgG secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... or polarized to Th1 phenotype by culturing in T cell media additionally supplemented with IL-12 (20 ng/mL) and anti-IL-4 (10 µg/ml) (Biolegend). After 48 h ...
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bioRxiv - Immunology 2022Quote: ... and then cell surface staining was performed for 30 min at 4°C with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: XCR1 ...
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bioRxiv - Immunology 2019Quote: ... GolgiPlug™ and GolgiStop™ were added 3 h before cells were processed for surface and intracellular staining for CD4 (BV785-conjugated anti-human; OKT-4, BioLegend), CD8 (APC-H7-conjugated anti-human ...
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bioRxiv - Immunology 2019Quote: ... and incubated for 1 h at 4°C in the dark: CD3 (anti-CD3-APC-780; clone 145-2C11, Biolegend), CD4 (anti-CD4-PE-Cy7 ...
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bioRxiv - Synthetic Biology 2020Quote: ... the media-trypsin mix was aspirated and the cells were fixed via incubation in 50 µL of 4% formaldehyde (BioLegend) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with either anti-beta amyloid 6E10 (mouse, 1:1,000 dilution of 1mg/mL stock) (Biolegend, USA), anti-tau MC1 (mouse ...
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bioRxiv - Immunology 2019Quote: ... to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience, Biolegend, Thermofisher Scientific or Miltenyi biotec ...
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bioRxiv - Bioengineering 2020Quote: ... and then cells were fixed with 4% PFA at room temperature for 10 minutes and permeabilized with intracellular staining perm wash buffer (BioLegend), and stained with the appropriate intracellular antibodies ...
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bioRxiv - Synthetic Biology 2021Quote: ... The samples were then flash frozen and defrosted for 4 rounds to lyse the cells and used on an IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Bioengineering 2020Quote: ... cells were recovered from wells and were incubated at 4°C for 30 minutes with antiCD3 antibody (Biolegend, CA, USA) and then were examined by flow cytometry.
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bioRxiv - Immunology 2021Quote: ... cells were blocked for 1 hour at 37⁰C with 300µl of Duolink® blocking solution then stained overnight at 4⁰C with purified mouse anti-Phosphoserine (Biolegend) or purified mouse IgG1 isotype control (clone MG1-45 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed with FACS buffer and labeled with PE conjugated mouse anti-human TNFRSF12A antibody (clone ITEM-4, Biolegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend. All final reactions were developed with TMB substrate (Thermo scientific ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes from uninfected or infected mice were incubated at 37C° in 5% CO2 for 4 h in medium containing Monensin and Brefeldin A solutions (Biolegend). Cells were then stained for surface antigens (TCRβ-PacificBlue and NK1.1-PE) ...
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bioRxiv - Immunology 2021Quote: Cell samples went through a series of centrifugations (400g, 5 minutes, at 4°C) and were stained with PE anti-CD45 (30-F11, BioLegend) in staining buffer (see flow cytometry section ...
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bioRxiv - Immunology 2021Quote: ... isotype controls and inhibitor of VLA-4 were used at 1 μg/ml: LEAF™ purified anti-CD29 (clone HMβ1-1, 102209, BioLegend), LEAF™ purified anti-CD31 (clone MEC13.3 ...
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bioRxiv - Immunology 2020Quote: ... Slides were incubated with monoclonal antibodies overnight at 4°C using anti-CD3 (100202, clone 17A2, BioLegend, San Diego, CA) to detect T-cells ...
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bioRxiv - Neuroscience 2021Quote: ... Except for immunohistochemistry for SOX-9 in which a β-gal staining was performed at 37°C for 3h followed by fixation with PFA 4% for 30 min at RT and incubation with unmasking antigen solution (927901; Biolegend). Sections were blocked and permeabilized in a permeabilization solution for 2h and incubated in goat polyclonal antibodies against SOX-9 (1:40 ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured for 4 h at 37°C in B cell medium containing 1x Cell Activation Cocktail (without Brefeldin A) (BioLegend) and 3h with 1x Protein Transporter Inhibitor Cocktail (ThermoFisher) ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Cetuximab (#A2000 ...
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bioRxiv - Immunology 2022Quote: ... or spleen were resuspended in MACS buffer and stained for 20 minutes at 4 degrees with the following fluorescently conjugated antibodies (BioLegend): anti-CD4 (clone RM4-5) ...
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bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed in 1X perm buffer for 20-30 min at 4°C using the following conjugated antibodies all purchased from BioLegend: mouse anti-IFN-γ (clone XMG1.2) ...
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bioRxiv - Immunology 2022Quote: ... and 30 minutes at 4°C with the following surface antibodies: CD8 PerCP-Cy5.5 (clone 53–6.7, Biolegend, cat#100734), CD44 BV650 (clone IM7 ...
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bioRxiv - Microbiology 2022Quote: ... They were then washed once with the permeabilisation buffer of the staining buffer set and stained with anti-Tax-AF647 (clone LT-4) and either anti-Ki-67-PE (BioLegend) or anti-γ-H2AX-PE (clone 2F3 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with primary antibodies diluted in blocking solution: anti-βIII Tubulin (1/500, Biolegend), anti-RSK2 (1/100 ...
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bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
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bioRxiv - Neuroscience 2022Quote: ... according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies: mouse anti-CD9 (1:1000; Biolegend), mouse anti-CD63 (1:1000 ...