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Citations for BioLegend :
1001 - 1050 of 1839 citations for 6 HYDROXY 2 METHYL PYRIMIDINE 4 ACETIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... the gels were incubated with 2 μg/ml Calcein-AM (BioLegend, 425201) and 1 μg/ml Propidium Iodide (MP Biomedicals ...
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bioRxiv - Neuroscience 2023Quote: ... or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend, 148603) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... anti-CD138 Alexa Flour 647 (1:80 dilution, clone 281-2, BioLegend), donkey anti-mouse IgG Alexa Fluor Plus 405 (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were collected and analyzed for IL-2 by ELISA (BioLegend).
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bioRxiv - Immunology 2023Quote: ... Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS; BioLegend) and incubated with PGP9.5 for 20 min at RT ...
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bioRxiv - Bioengineering 2024Quote: ... and cells were cultured with 0.5 µg/mL recombinant IL-2 (BioLegend) in RPMI + 10% heat-inactivated FBS for 24 h before use in NK cell cytotoxicity or flow cytometry experiments.
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bioRxiv - Biochemistry 2024Quote: ... An anti-pERK1/2 (Thr202/Tyr204) antibody (6B8B69) was purchased from BioLegend. Dead cells were stained with the LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit for 633- or 635-nm excitation (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... 2 hours after start of restimulation 5μg/mL of Brefeldin A (BioLegend) was added to each well for the remaining 3 hours ...
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bioRxiv - Microbiology 2022Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Immunology 2021Quote: ... prior to staining for 1 h at 4°C with the following antibodies from BioLegend (most) or Novus Biologicals (MARCO ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated overnight at 4 °C with the primary antibodies against ASC (HASC-71, BioLegend). Cells were washed 3 times with washing buffer (1x PBS and 0.025% of Tween20 ...
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bioRxiv - Genomics 2019Quote: ... cells were stained in batches of 1×107 cells for 30 min at 4°C (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Immunology 2019Quote: Neutrophils obtained after 4-day differentiation (“in vitro-derived”) were washed and labeled using CFSE (BioLegend). Murine bone marrow neutrophils were isolated by negative selection (StemCell Technologies ...
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bioRxiv - Genomics 2021Quote: ... This was followed by 30min incubation at 4 °C with a Streptavidin-PE (Biolegend, 1:5000), anti c-kit-APC (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... After 4 hours BMDMs were harvested and stained with an antibody against F4/80 (BM8, Biolegend) and propidium iodide (BD Biosciences) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse B220 (Biolegend, APC), GL7 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were incubated for 15 minutes at 4°C in streptavidin-PE/Cy7 (BioLegend) to detect NCAM-biotin ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were first incubated for 1h at 4°C with 2μl of anti- HA antibody (BioLegend) and then for another hr after adding 30μl of washed magnetic ProteinG beads (Cytiva) ...
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Two subsets of human marginal zone B cells resolved by global analysis of lymphoid tissues and bloodbioRxiv - Immunology 2021Quote: ... Cells were then incubated for 10 minutes at 4°C with True stain Fc block (BioLegend) and washed ...
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bioRxiv - Immunology 2019Quote: ... macrophage activation was induced by adding either IL-4 (20 ng/ml, Biolegend, San Diego, USA), IL-13 (20 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by overnight staining at 4°C with rat anti-mouse CD45 primary antibody (103102, Biolegend) diluted 1:500 in 1x D-PBS ...
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bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Concentrations of IFN-γ and IL-4 in supernatants were analyzed by DuoSet ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... that had previously been linked with 4 μg each of mouse monoclonal 6E10 and 4G8 (Biolegend) were added to the neutralized FA fraction ...
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bioRxiv - Neuroscience 2023Quote: ... supernatant was removed by aspiration and 500 microliters of fixation buffer (4% paraformaldehyde in PBS, Biolegend) added to each well and the slide kept in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and stained (30 min at 4°C) with PE anti-TLR4 (Biolegend; clone Sa15-21; 145404) in blocking buffer (PBS with 2% rat serum) ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... at 10 ng/mL (growth media) and 4 µg/mL of anti-CD28 antibody (Biolegend, #302934). Cells were plated in non-TC treated 24-well plates previously coated with 2 µg/mL of anti-CD3 antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... 10 µg/ml of Ultra-LEAFTM purified anti-mouse IL-4 antibody (clone 11B11, Biolegend, #504122), 10 ng/ml animal-free recombinant murine IFNγ (PeproTech ...
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bioRxiv - Genetics 2021Quote: ... production from LPS- or OL-treated BMDM were performed using Mouse TNF-α ELISA MAX Deluxe kit and Mouse IL-6 ELISA MAX Deluxe kit (BioLegend, San Diego, CA, USA). Follow-up cytokine (IL1β ...
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bioRxiv - Immunology 2021Quote: ... anti-IgD-FITC (1:20 dilution, clone IA6-2, cat. no. 348206, BioLegend), anti-IgM-FITC (1:20 dilution ...
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bioRxiv - Immunology 2019Quote: ... αIL-2 (clone MQ1-17H12) and α-IFN-α (clone MAb11) (all Biolegend). Near-IR (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2, Biolegend). RNAi knockdown of DCAF1 was performed as described before52 using ON-TARGET plusHuman DCAF1 siRNA - SMARTpool (Dharmacon ...
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bioRxiv - Bioengineering 2022Quote: ... and 50 μg/mL anti-IL-2 Alexa Fluor 488 (Biolegend, Catalog: 500314) in 50μL washing buffer per 6 μL nanovial volume at 37°C for 30 minutes protected from light ...
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bioRxiv - Immunology 2019Quote: ... Supernatant was collected for IL-2 measurement using an ELISA kit (BioLegend #431801).
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bioRxiv - Immunology 2020Quote: ... Supernatants were collected to measure IL-2 by ELISA (ELISA MAX Deluxe, BioLegend) and proliferation assessed by methyl-3[H]-thymidine (1 µCi/well ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... and a SARS-CoV-2 nucleocapsid antibody (clone A20087H, mouse IgG2b isotype, BioLegend) followed by a secondary antibody stain with PE-Cy7-conjugated anti-mouse IgG2b (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... CD46 APC (targets MCP protein) clone TLA-2-10 (cat# 352405, BioLegend®), CD49d APC (targets α4 integrins ...
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bioRxiv - Microbiology 2021Quote: ... rat anti-mouse CD138-BV605 (clone 281-2, 1:200, Biolegend, cat#142516), rat anti-mouse CD19-BV785 (clone 1D3 ...
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bioRxiv - Immunology 2019Quote: ... or CD206-(Alexa Fluor-647 rat anti-mouse #321116 (Clone 15-2, BioLegend) conjugated antibodies (1:200) ...
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bioRxiv - Cell Biology 2019Quote: ... 2% FBS using the following antibodies: CD45.2-APCCy7 (Biolegend, Ref#109824, clone 102), CD45.1-FITC (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... 2 μg/ml rat anti-mouse CD40 antibody (BioLegend, San Diego, California, USA), 2 ng/ml interleukin 7 (IL7 ...
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bioRxiv - Immunology 2021Quote: ... and seeded into 2 μg/mL anti-CD3 antibody (clone: 145-2C11, BioLegend) pre-coated 24-well plates ...
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bioRxiv - Immunology 2022Quote: ... pooled 8:2 CD45+:CD45-cells were resuspended in Cell Staining Buffer (BioLegend) and stained with a pool of 137 TotalSeq-C antibodies (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 2 μl PE/Cyanine7 anti-human IgM antibody (BioLegend, 314532, clone: MHM-88), 2 μl PE/Cyanine7 anti-human IgD antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Recombinant human SARS-CoV-2 spike S1 IgG lyophilized antibody (Clone AM009105, BioLegend) was used as a standard.
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bioRxiv - Cancer Biology 2024Quote: ... 100 µL of PE goat anti-mouse IgG (BioLegend #405307; 2 ug/mL) added and incubated in the dark for 30 min with shaking (800 rpm) ...
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bioRxiv - Immunology 2024Quote: ... H2-Dd-FITC (1:200; clone 34-2-12; BioLegend Cat. No. 110606), H2-Kd-APC (1:160 ...
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bioRxiv - Immunology 2024Quote: ... washed twice HBSS containing 2% FCS and incubated with Human Trustain Fcx (Biolegend) for 20 min on ice ...