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Citations for BioLegend :
1101 - 1150 of 8410 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and OX40 PE-Cy7 (2:100; Biolegend Cat#350012). All samples were acquired on ZE5 cell analyzer (Bio-rad laboratories ...
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bioRxiv - Immunology 2023Quote: ... anti-human IgD-PerCP/Cy5.5 (clone IA6-2, BioLegend), anti-human IgD-Alexa Fluor 700 (clone IA6-2 ...
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bioRxiv - Cell Biology 2023Quote: ... macrophages or granulocytes (CD14, Sa14-2-APC, #123311, BioLegend and CD11b ...
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bioRxiv - Immunology 2024Quote: ... PE/Cyanine7 anti-mouse CD49d Antibody (R1-2, Biolegend), PE-Cy7 Rat Anti-Mouse CD62L (MEL-14 ...
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bioRxiv - Immunology 2024Quote: ... in Panel 2: CD3-FITC (Biolegend, UCHT1, Cat. 300406), CD4-PE-Cy7 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... PE/Cyanine7 anti-mouse CD49d Antibody (R1-2, Biolegend), APC Mouse anti-Mouse CD45.2 (104 ...
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bioRxiv - Immunology 2024Quote: ... or plate-bound 11-CD3 (BioLegend; 2 µg/mL) and plate-bound CD155-Fc (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and soluble αCD28 (2 μg/ml, clone 37.51, BioLegend) antibodies ...
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bioRxiv - Immunology 2024Quote: ... TCRαβ PEdazzle594 (2 μl per test, clone IP26, Biolegend) and TCRγδ BV421 (2 μl per test ...
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bioRxiv - Immunology 2024Quote: ... CD3 AF488 (2 μl per test, clone UCHT1, Biolegend), CD3 AF700 (3 μl per test ...
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bioRxiv - Cell Biology 2024Quote: ... and supplemented with 2 μg/mL αCD28 (Biolegend, #102116). The cell suspension was distributed over a 96-well plate (200 μL/well ...
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bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following anti-mouse antibodies we used for surface staining: Alexa Fluor 647-labeled H-2Kb/H-2Db – MHC Class I (clone 28-8-6, Biolegend); PE-labeled I-A/I-E – MHC Class II (Clone M5/114.15.2 ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Phenotypes of B cells before and after 6 days of culture were determined by flow cytometric analysis using mouse monoclonal antibody clones: CD19 PECy7 HIB19 (Biolegend), CD27 BV421 O323 (Biolegend) ...
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bioRxiv - Genomics 2020Quote: ... 1.25 × 10^6 PBMCs were spun down and resuspended in 25 μl CITEseq antibody cocktail (TotalSeq™-A Antibodies, BioLegend) containing among others one hashing antibody per each sample ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Immunology 2024Quote: ... derived from OT-1 mice treated with either CATR or vehicle were restimulated with 1µg/ml OVA-peptide for 6 hours and Brefeldin A (Biolegend, 420601) for 4hr prior to analysis.
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bioRxiv - Molecular Biology 2024Quote: IL-6 KO mice with cast immobilization for 24 h were subjected to intraperitoneal administration of 400 ng of rIL-6 (575702, BioLegend) in saline or of saline alone ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured at a density of 0.5x10^6 cells/ml in complete RPMI medium and stimulated with plate-bound αCD3 (10 μg/ml, clone 145-2C11, BioLegend) and soluble αCD28 (2 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4 and CD8 T cells were isolated from the spleen and peripheral LNs of C57BL/6 mice using the MojoSort™ Mouse CD4 or CD8 Naïve T Cell Isolation Kit from BioLegend and irradiated with 254 nm-UV light (600 mJ/cm2 using a Stratalinker 2400) ...
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bioRxiv - Immunology 2024Quote: ... Fluorescently labeled anti-mouse antibodies against CD45 (104) and H-2Kb/H-2Db (28-8-6) were obtained from BioLegend.
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bioRxiv - Immunology 2024Quote: ... bone marrow progenitor cells were harvested from the femur of C57BL/6 mice and plated at a density of 3×106/3mL well on 6-well plates with 20 ng/mL GM-CSF (BioLegend) in RPMI 1640 medium (Wisent ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were isolated from the femurs of 6-week-old Balb/c mice (Taconic Laboratories) and differentiated by growth in (BioLegend) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2024Quote: ... All other cytokines were assessed using Mouse M1 Macrophage LEGENDplex flow-based assay (CXCL1, IL-1β, IL-6, IL-12p40, IL-12p70, IL-18, IL-23, TNFα; BioLegend) with BD LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Genomics 2024Quote: ... Cells were then cultured for 12-18h in DMEM/10%FBS supplemented with 100ng/mL mSCF/mIL-3/mIL-6 (BioLegend) at 37°C at 5% CO2 [Step Four] ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed twice with 2% FACS buffer (1X PBS and 2% BSA) centrifuged and stained with human TruStain FcX (Biolegend, San Diego, CA) for 20 minutes ...
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bioRxiv - Systems Biology 2024Quote: Mice were injected retro-orbitally (under isoflurane anesthesia 2 minutes before euthanasia) with 2 μg of phycoerythrin (PE)–conjugated anti-CD45 antibody (Biolegend, clone 30-F11) to label intravascular leukocytes ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were applied overnight at 4°C and included SMI31 (Biolegend, 801601), IBA1 (Wako ...
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bioRxiv - Immunology 2021Quote: ... MDM were suspended in 100μL of 4°C annexin V binding buffer (BioLegend) and the two pHrodo loaded sets were stained with 2μL of FITC Annexin V (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then fixed in 4% paraformaldehyde and permeabilized using Permeabilization buffer (Biolegend). Intracellular proteins were stained with antibodies against TNF (MP6-XT22 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with Human TruStain FxC (10 minutes at 4°C; Biolegend, #422302) to block Fc receptor-mediated binding ...
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bioRxiv - Immunology 2020Quote: ... and cultured for 4 days +/- αIL10 antibody +/- isotype control (5μg/ml, both Biolegend). Assessment of the direct effect of IL-10 on human CD4 T cells was performed by plate bound αCD3/28 (1μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Remaining volume was incubated at 4°C with anti-HA antibody (Biolegend, #901513) at 1:150 dilution for 4hrs on a gentle spinner ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed for 10 min at 4°C using IC fix (Biolegend) and cells were then incubated in for 20 min at RT in Permeabilization buffer (biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... we added 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6). We imaged the nanowell using Zeiss Axio Observer Z1 microscope equipped with 20x/0.8 NA objectives and a Hamamatsu Orca Flash v2 camera.
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and stained with CTLA-4-PE-Dazzle 594 (Biolegend), Ki67-PerCP-eFluor 710 (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304, BioLegend), and 10 μg/mL anti-mouse IL-12/23p40 antibody (clone C17.8 ...
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bioRxiv - Developmental Biology 2023Quote: ... additional staining with anti-CD34 (4 µL CD34 APC/Cy7, clone: 581, Biolegend) and anti-CD14 antibodies (3 µL CD14 PE-CF594 ...
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bioRxiv - Immunology 2023Quote: ... 4 µL of cocktail containing PMA/ionomycin + Brefeldin A (Biolegend, San Diego, CA) was added ...
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bioRxiv - Neuroscience 2024Quote: ... coated with 4 µg 77G7 anti-tau antibody (MTBR epitope 316-355, BioLegend) per sample ...
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bioRxiv - Immunology 2024Quote: CD9 antibody (Clone: HI9a, Biolegend, Cat-no. 312102; 4 μl in 1ml PBS) was used to coat a 48-well plate ...