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Citations for BioLegend :
1201 - 1250 of 8410 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Immunology 2024Quote: ... Lysis reaction was stopped by adding 10ml of PBS/2mM EDTA + 2% FBS and the resuspended solution was centrifuged 5min at 350g.Spleen was taken from 8- to 16-week-old mice and pressed in a 6 wells plate containing 2ml of red blood lysis buffer (Biolegend #420302) 2 more ml were added and then incubated 5min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA ...
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bioRxiv - Cancer Biology 2024Quote: Spleen was isolated from C57BL/6 mice and CD8 T cells were isolated using MojoSort™ Mouse CD8 T Cell Isolation Kit (Biolegend). The cells were stimulated with Dynabeads™ Mouse T-Activator CD3/CD8 kit (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... - β1 (clone HMb1-1, 1:200) (Biolegend), were also employed ...
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bioRxiv - Microbiology 2020Quote: ... and 50 ng/ml GM-CSF + 500 U/ml IL-4 (Biolegend, https://www.biolegend.com), respectively ...
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bioRxiv - Genomics 2021Quote: ... followed by incubation for 24 h at 4 °C with anti-Cas9 (BioLegend, 844301) primary antibody diluted in wash buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Coverslips were then incubated overnight at 4 degrees with primary antibody-anti-Vimentin (Biolegend). Coverslips were washed and stained with secondary antibody ...
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bioRxiv - Genomics 2022Quote: ... at 4°C for 25 minutes and washed thrice with Cell Staining Buffer (BioLegend) and pooled equally as described previously18 ...
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bioRxiv - Cancer Biology 2023Quote: ... and cultured for 4 days prior to staining with anti-CD8-APC (SK1, BioLegend) and proliferation measured by flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Cells were placed in 4 ml of 1X annexin binding buffer (Biolegend, cat#: 422201) with biotinylated annexin V (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... The PE-conjugated antibody for murine TLR-4 antibody (145404) was purchased from BioLegend. anti-TNFR1 (Armenian hamster IgG ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with the anti-mouse IFNγ AN18 antibody (Biolegend) at 2 μg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112, BioLegend) and anti-Gb1-4 (sc-378 ...
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bioRxiv - Immunology 2023Quote: ... tissue sections were stained O/N 4°C with either anti-CD11c (N418, Biolegend) and anti-CD63 (NVG-2 ...
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bioRxiv - Immunology 2022Quote: ... mice received an intravenous injection of 3ug of anti-CD4 (RM4-4, Biolegend 116022) antibody and were euthanized 3 minutes after injection.
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bioRxiv - Immunology 2022Quote: Mice received an intravenous injection of 3ug anti-CD4-biotin (RM4-4, Biolegend 116010) antibody 3 minutes prior to euthanasia ...
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bioRxiv - Immunology 2022Quote: ... cells were re-stimulated for 4 hr with PMA/Ionomycin and Brefeldin A (Biolegend), according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... After 4 days of co-culture cells were collected stained for CD8-PECy5 (BioLegend) and assessed for cell proliferation with flow cytometry ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked with TruStain FcX (α-mouse CD16/32; 10 min, 4°C; #101320, BioLegend) and stained (20 min ...
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bioRxiv - Immunology 2023Quote: ... Activation was measured by the following markers: CD137 APC (4:100; Biolegend Cat# 309810) and OX40 PE-Cy7 (2:100 ...
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bioRxiv - Biophysics 2024Quote: ... blots were incubated overnight at 4°C with anti-ASIC1 primary antibody (BioLegend #833501) at a 1:1000 dilution ...
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bioRxiv - Immunology 2024Quote: ... Capture and biotin conjugated detection anti-mouse IL-4 antibodies were purchased from BioLegend. Anti-mouse IL-13 antibody pair were purchased from Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: WT mice were retro-orbitally injected with 4 μg CD45-FITC (Cat. #103108; Biolegend). Mice were then allowed to recover ...
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bioRxiv - Cancer Biology 2024Quote: ... for 4 minutes before stopping the reaction with 25 µl of Stop Solution (BioLegend). We analyzed the plates on an Infinite m200 microplate reader at a wavelength of 450 nm (Tecan) ...
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T cells were isolated from the spleens of C57BL/6 mice or IL17-GFP mice using a MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend cat. 480040). T cells from C57BL/6 mice were further labeled with CellTrace™ Violet (Invitrogen cat ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from the spleens of C57BL/6 mice using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience ...
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bioRxiv - Microbiology 2023Quote: Cytokine levels in the serum and spleen of mice infected with SPN were measured using ELISA MAX™ Standard Set Mouse IL-6 (BioLegend 431301) and ELISA MAX™ Standard Set Mouse IL-1β (BioLegend 432601 ...
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bioRxiv - Immunology 2023Quote: ... IFN-α and IL-6 were detected by the Mouse Anti-Virus Response Panel (13-plex) (Cat# 740622 BioLegend, San Diego, CA) and analyzed on the NovoCyte Flow Cytometer (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... IL-6 and IL-15 was quantified in cell supernatants using a sandwich ELISA Max Deluxe Set Human (Biolegend, San Diego, CA), according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2024Quote: ... we extracted the blood vessels and popliteal lymphatic vessels and hashed the samples with TotalSeq™-A0306 anti-mouse Hashtag 6 Antibody (Biolegend, 155811) and TotalSeq™-A0305 anti-mouse Hashtag 5 Antibody (Biolegend ...
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bioRxiv - Microbiology 2024Quote: ... anti-PSGL-1 (KPL-1) (1:1000) (BioLegend); anti-HIV-1 p24 antibody (183-H12-5C ...
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bioRxiv - Cancer Biology 2021Quote: ... and Alexa Fluor 647-conjugated BCL-2 (BioLegend Cat# 633510, RRID:AB_2274702). PE-Cy7-conjugated Streptavidin was used to detect biotinylated CD49d (9C10).
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bioRxiv - Immunology 2021Quote: ... anti-mouse mouse MHC H-2 hashtag antibodies (Biolegend TotalSeq™) were used to label separate FACS-purified subsets which were washed ...
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bioRxiv - Immunology 2021Quote: ... The antibody against Bcl-2 (BCL/10C4) was purchased from Biolegend. The antibodies against TCF1 (C63D9 ...
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bioRxiv - Bioengineering 2021Quote: ... and APC anti-human CD46 (clone TRA-2-10, Biolegend 352405). Antibody staining was performed for 20 minutes on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... washed with cold 2 ml staining buffer (BioLegend Inc, catalog # 420201) and resuspended in cold Annexin V binding buffer at 1×106 cells per ml ...
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bioRxiv - Immunology 2022Quote: ... PMN were stained with 2 μl of Zombie Aqua dye (BioLegend) diluted 1:100 for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... AM were imaged PE-Siglec F (2 µg, clone S17007L; BioLegend) administered intravenously 30 mins after engraftment ...
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bioRxiv - Immunology 2022Quote: ... APC-anti human 2B4/CD244 (2-69; BioLegend, San Diego, CA); or with the isotype control APC mouse IgG1 (MOPC-21 ...
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bioRxiv - Immunology 2022Quote: ... anti-CD138-PE (281-2) and PE/Cy7- Streptavidin from Biolegend, and anti-FCRL5-Alexa488 and anti-FCRL5-APC (Polyclonal ...
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bioRxiv - Bioengineering 2020Quote: All reagents for detection of IL-2 were purchased from Biolegend. Supernatants were collected after 16 hours of coculture of 5MCAR 58α−β− cells and M12 target cells ...
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bioRxiv - Immunology 2020Quote: ... CD19 Pacific Blue (clone H1B19, BioLegend cat#302224, 2:100 dilution); CD3 AF700 (clone OKT3 ...
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bioRxiv - Immunology 2020Quote: ... CD4 PerCP-Cy5.5 (clone OKT4, BioLegend, cat# 317428, 2:100 dilution); CD127 AF488 (clone A019D5 ...
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bioRxiv - Immunology 2021Quote: ... or 5 μg/ml anti-human TRAIL antibody (RIK-2, Biolegend). After 2 h of preincubation effector cells were added to target cells.
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bioRxiv - Systems Biology 2020Quote: ... supplemented with 2 μg/ml of anti-CD28 (clone CD28.2, Biolegend) and with 10 ng/ml of recombinant IL-2 (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... followed by the addition of biotinylated anti-mouse IL-2 (BioLegend), and then streptavidin-horseradish peroxidase conjugate (SA-HRP ...
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bioRxiv - Immunology 2023Quote: ... and soluble anti-CD28 antibody (2 ug/mL, Clone: CD28.2, Biolegend) in the culture medium containing 1×Brefeldin A (Biolegend ...