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Citations for BioLegend :
901 - 950 of 8410 citations for 2 1H Imidazol 1 yl 6 methyl 4 pyrimidinemethanamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Donor 5 and 6 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with inhibitors for 6 hours and then stained with Zombie Aqua (BioLegend, Cat. # 423101) following the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... TNF-α and IL-6 protein were quantified using ELISA MAX Deluxe kits (BioLegend, San Diego, CA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... parasites were stained with 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester (CFSE; Cell Division Tracker Kit, BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Supernatants were collected and assessed for TNF-α and IL-6 using deluxe ELISA kits from Biolegend. 10mg of perfused liver tissue was rinsed with cold PBS ...
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bioRxiv - Immunology 2024Quote: ... IL-6 and IL-10 were determined by a LEGENDplex multiplex assay (mouse inflammation panel, Biolegend, 740446), all according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... anti-Siglec-7–APC/Fire 750 (clone 6-434) and anti-TIGIT-BV421 (clone A15153G) from BioLegend; (ii ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and resuspended at 2×106 in 100μl PBS with 2% FBS and incubated with Zombie violet fixable viability dye (Biolegend) and an antibody cocktail for 25min at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: C57BL/6 mice were treated twice with topical R848 and then were injected once I.P with 2 mg of (5-bromo-2’-deoxyuridine) BrdU (Biolegend). Bone-marrow (BM ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Immunology 2024Quote: ... for the clone that binds to the high affinity IL-2 receptor (CD25): we incubated 0.5ugr mouse Il-2 (PepreoTech) to 5ugr of an IL-2 mAb (JES6-1A12, Biolegend) for 30 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... The amount of IL-2 in the culture medium was measured using a mouse IL-2 ELISA kit (Biolegend).
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bioRxiv - Bioengineering 2024Quote: ... APC- CD81 (clone Eat-2; 2 µg/mL) detection antibodies or isotypic controls (all antibodies were purchased from BioLegend). Flow cytometry was performed on a MACSQuant 10 instrument (Miltenyi Biotech ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 and anti-IFN-γ (5 μg/mL, Biolegend) for 3 days.
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bioRxiv - Immunology 2021Quote: ... plates were coated with anti-mouse IL-4 antibody (11B11, Biolegend) then blocked as above ...
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bioRxiv - Immunology 2022Quote: ... and 4 μL of anti-CD193-PerCP/Cy5.5 (clone 5E8, BioLegend), and incubated the sample for ~18 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml IL-4 + 20 ng/ml IL-13 (Biolegend) for polarization to M2 ...
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bioRxiv - Immunology 2020Quote: ... Bcl6 PE/Dazzle (clone 7D1, BioLegend, cat# 358510, dilution 4:100). Data was recorded on a FACSymphonyTM flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... IL-4 (11B11) and CD124 (I015F8) mAbs were purchased from BioLegend. Anti-CD44 (IM-7) ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 4 times with washing buffer (BioLegend; Cat: 4211601) and then incubated for 1 h at RT with a 1:2000 dilution of secondary antibody of horse radish peroxidase (HRP)-conjugated rabbit anti-mouse IgM (μ chain) ...
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bioRxiv - Immunology 2020Quote: ... or 40 ng/mL IL-4 (Biolegend, San Diego, CA, USA) in duplicates respectively for 24 hours in X-VIVO 15 serum free medium ...
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bioRxiv - Immunology 2022Quote: ... and 10 μg/mL anti-IL-4 Ab (clone 11B11, BioLegend) were added to the culture media ...
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bioRxiv - Cancer Biology 2022Quote: ... we stained the cells with 4 μg/ml PE-streptavidin (BioLegend) at the 37 °C for 45 min ...
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bioRxiv - Immunology 2022Quote: ... 5 μg/ml of anti-IL-4 (11B11) mAb (Biolegend, USA), 5 ng/ml of IL-2 recombinant (eBioscience ...
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bioRxiv - Immunology 2022Quote: Mouse splenocytes were stained with 4 μg/ml LAG3 BV785 (BioLegend) and 4 μg/ml CD49b PeCy7 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... anti-IL-4 (#500808) was from BioLegend (San Diego, CA, USA). Cells detached from culture plates were filtered through 70-μm cell strainers ...
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bioRxiv - Pathology 2023Quote: ... Neutrophils were maintained 4 hours in culture with Brefeldin A (Biolegend) to stop exocytosis.
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bioRxiv - Bioengineering 2023Quote: ... cells were fixed with 4% paraformaldehyde (Biolegend, San Diego, CA, USA) for 10 minutes and subsequently permeabilized with 0.1% Triton X100 (Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.1% Triton-X and 4 µg/mL DAPI (#422801, BioLegend, USA) for 1 h at RT and stained with two staining panels ...
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bioRxiv - Microbiology 2023Quote: ... PE/Cyanine7 anti-mouse IL-4 antibody (BioLegend, catalog no. 504118), and Brilliant Violet 711 anti-mouse IL-17A Antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
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bioRxiv - Immunology 2023Quote: ... after stimulating cells for 4 hours with Brefeldin A (Biolegend #420601). Absolute cell counts were determined using counting beads (Biolegend #424902) ...
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bioRxiv - Cell Biology 2023Quote: ... TotalSeq™-A0304 anti-mouse Hashtag 4 Antibody) (BioLegend, TotalseqA antibodies). The 4 biological replicates of each time point were stained with one of the 4 unique hash antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... FITC anti-mouse CD4 Antibody (BioLegend, catalog #116003, clone RM4-4), PerCP/Cyanine5.5 anti-mouse CD4 Antibody (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... KO iv-: TotalSeq-A0304 anti-mouse Hashtag 4 (all from Biolegend). Equal number of cells from each population were pooled and then subjected to 10X single cell RNA seq library preparation targeting 1×104 total cells ...
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bioRxiv - Immunology 2024Quote: ... BMDMs were stimulated on day 5 with either IL-4 (Biolegend) at 20 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Frozen PBMC of post-CA patients were thawed and incubated in media (RPMI1640 + 2% FBS) with recombinant human Nectin-2 (Biolegend) or isotype control (ENZO ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2021Quote: ... containing anti-CD9 (human HI9a, mouse MZ3), anti-CD63 (human H5C6, mouse NVG-2), anti-CD81 antibodies (human 5a6, mouse Eat-2) from Biolegend, and anti-human ACE2 (R&D Biosystems cat no ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions were washed after collection and stained in ice-cold Ca+2/Mg+2-free PBS with a viability dye (Zombie NIR, BioLegend) for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed twice in FACS buffer (PBS + 2% FBS + 1mM EDTA) before staining with anti-pERK1/2 (Biolegend 369506 100x ...
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...