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Citations for BioLegend :
951 - 1000 of 1849 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... All other cytokines were assessed using Mouse M1 Macrophage LEGENDplex flow-based assay (CXCL1, IL-1β, IL-6, IL-12p40, IL-12p70, IL-18, IL-23, TNFα; BioLegend) with BD LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Genomics 2024Quote: ... Cells were then cultured for 12-18h in DMEM/10%FBS supplemented with 100ng/mL mSCF/mIL-3/mIL-6 (BioLegend) at 37°C at 5% CO2 [Step Four] ...
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bioRxiv - Immunology 2024Quote: Murine DCs were differentiated from isolated bone marrow via culture in RPMI + 10% FCS 1% Pen/Strep and 1% Amino Acids containing 20 ng/ml GMCSF (Biolegend, #576306) on silicone hydrogels with different stiffnesses ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were applied overnight at 4°C and included SMI31 (Biolegend, 801601), IBA1 (Wako ...
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bioRxiv - Immunology 2021Quote: ... MDM were suspended in 100μL of 4°C annexin V binding buffer (BioLegend) and the two pHrodo loaded sets were stained with 2μL of FITC Annexin V (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then fixed in 4% paraformaldehyde and permeabilized using Permeabilization buffer (Biolegend). Intracellular proteins were stained with antibodies against TNF (MP6-XT22 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with Human TruStain FxC (10 minutes at 4°C; Biolegend, #422302) to block Fc receptor-mediated binding ...
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bioRxiv - Immunology 2020Quote: ... and cultured for 4 days +/- αIL10 antibody +/- isotype control (5μg/ml, both Biolegend). Assessment of the direct effect of IL-10 on human CD4 T cells was performed by plate bound αCD3/28 (1μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Remaining volume was incubated at 4°C with anti-HA antibody (Biolegend, #901513) at 1:150 dilution for 4hrs on a gentle spinner ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed for 10 min at 4°C using IC fix (Biolegend) and cells were then incubated in for 20 min at RT in Permeabilization buffer (biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... we added 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6). We imaged the nanowell using Zeiss Axio Observer Z1 microscope equipped with 20x/0.8 NA objectives and a Hamamatsu Orca Flash v2 camera.
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and stained with CTLA-4-PE-Dazzle 594 (Biolegend), Ki67-PerCP-eFluor 710 (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304, BioLegend), and 10 μg/mL anti-mouse IL-12/23p40 antibody (clone C17.8 ...
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bioRxiv - Developmental Biology 2023Quote: ... additional staining with anti-CD34 (4 µL CD34 APC/Cy7, clone: 581, Biolegend) and anti-CD14 antibodies (3 µL CD14 PE-CF594 ...
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bioRxiv - Immunology 2023Quote: ... 4 µL of cocktail containing PMA/ionomycin + Brefeldin A (Biolegend, San Diego, CA) was added ...
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bioRxiv - Cell Biology 2024Quote: ... incubated overnight at 4°C with primary antibodies (anti-HA, 1:1000, BioLegend, #901502 ...
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bioRxiv - Neuroscience 2024Quote: ... coated with 4 µg 77G7 anti-tau antibody (MTBR epitope 316-355, BioLegend) per sample ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice and resuspended in 1:4 fixation buffer (BioLegend 420801) in PBS for 10 min at room temperature ...
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bioRxiv - Immunology 2024Quote: CD9 antibody (Clone: HI9a, Biolegend, Cat-no. 312102; 4 μl in 1ml PBS) was used to coat a 48-well plate ...
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bioRxiv - Biochemistry 2024Quote: ... followed by overnight incubation at 4°C with 6E10 (BioLegend, Cat. No. 803002) or 4G8 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... monocytes were stimulated with IL-4 (50 ng/mL, BioLegend, catalog no. 574002). 50 µL of medium containing M-CSF and IL4 was replenished every other day for 7 days ...
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bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Developmental Biology 2021Quote: ... Surface staining was done by adding the flow cytometry antibody cocktail outlined in Extended Data Table 6 containing F4/80 (BioLegend, 123120), CD11b (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were induced to differentiate by culturing in the absence of β-Estradiol for 6 days before cells were stained for CD11b (Biolegend) and analyzed on the LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA ...
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bioRxiv - Immunology 2024Quote: ... Lysis reaction was stopped by adding 10ml of PBS/2mM EDTA + 2% FBS and the resuspended solution was centrifuged 5min at 350g.Spleen was taken from 8- to 16-week-old mice and pressed in a 6 wells plate containing 2ml of red blood lysis buffer (Biolegend #420302) 2 more ml were added and then incubated 5min at RT ...
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bioRxiv - Cancer Biology 2024Quote: Spleen was isolated from C57BL/6 mice and CD8 T cells were isolated using MojoSort™ Mouse CD8 T Cell Isolation Kit (Biolegend). The cells were stimulated with Dynabeads™ Mouse T-Activator CD3/CD8 kit (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used: CD4 (Clone RM4-5, BioLegend, Cat 100527), GMCSF (Clone MP1-22E9 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with 5 μL APC Annexin V (Biolegend: 640920) and 10 μL PI solution (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... 5 μL of 25 μg/mL anti-CD4 PE (Biolegend, Catalog: 344606) and 5 μL of 100 μg/mL anti-CD8 Alexa Fluor 488 (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... and 5 μg/mL of CD40 antibody ligand (clone: HM40-3; BioLegend).
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bioRxiv - Immunology 2022Quote: ... Cells were treated with 5 µg/mL of Brefeldin A (BioLegend, 420601) and allowed to incubate for 6 hours in a 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... We stimulated Jurkat cells with 5 μg/ml anti-CD3 (Biolegend-317315) and 100 ng/ml phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Neuroscience 2021Quote: ... and Rat IgG2a,k AF647 Isotype Control (RTK2758, 5 μg/mL, Biolegend).
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bioRxiv - Immunology 2021Quote: ... in the presence of plate- bound anti-CD3 (5 μg/mL, Biolegend), CpG (10 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD4 (RM4-5; BV 711) and PD-1 (J43; FITC; all BioLegend). After fixation and permeabilization (Foxp3 Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2022Quote: ... and blocked with 5 μg/mL anti-CD16/32 (clone 93; Biolegend) and heat inactivated normal mouse serum (1:10 ...
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bioRxiv - Immunology 2022Quote: ... single cell suspensions were incubated for 5 hours in 2μM monensin (BioLegend) to inhibit intracellular protein trafficking ...
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bioRxiv - Cancer Biology 2022Quote: ... and stimulated with 5 ug/mL Ultra-LEAF anti-human CD3 (Biolegend) and 2.5 ug/mL Ultra-LEAF anti-human CD28 (Biolegend) ...
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bioRxiv - Neuroscience 2024Quote: ... CD64-PE-594 (BioLegend, clone X54-5/7.1, cat #139319, 1:400), Ly6c-BV711 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... keratin 5 (1:2000, KRT5 for basal cells; BioLegend, Catalog number: 905503), SCGB1A1 (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Brilliant Violet 605 anti-mouse CD4 (Biolegend, Cat# 100548, Clone RM4-5), Fluorescein isothiocyanate (FITC ...