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Citations for BioLegend :
1201 - 1250 of 1849 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... CD8 BUV496 or BV650 (2:100 or 4:100; BD Biosciences Cat# 612942 or Biolegend Cat# 301042), and Live/Dead eFluor506 (5:1000 ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: ... Cells were permeabilised for 20 minutes at 4°C using the FOXP3 Fix/perm buffer set (Biolegend) containing purified rat IgG2b anti-mouse CD16/CD32 receptor antibody ...
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bioRxiv - Immunology 2022Quote: ... Samples were incubated overnight at 4°C with anti-CD15(HI90)-Bi209 (primary antibody purchased from Biolegend; conjugated with Fluidigm’s MaxPar labeling kit ...
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bioRxiv - Immunology 2024Quote: ... Staining with primary antibodies was performed overnight at 4°C in blocking buffer (LN: IgD (Biolegend, 405705), and CD8 (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... they were incubated at 4°C overnight in primary antibodies against anti-HA (1:100; BioLegend; 901533), CA3 (1:150 ...
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bioRxiv - Immunology 2023Quote: ... for 10min at 4°C, then FcR (Miltenyi, Cat. 130-092-575) and monocyte (Biolegend, Cat. 2000135006) blocking for 10min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... at 4°C) and stained with a fixable viability dye (Zombie NIR™ Fixable Viability Kit, Biolegend) in PBS for 20 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Intracellular IFN-γ and IL-4 were stained with anti-IFN-γ-PE/Cyanine7 (clone XMG1.2, BioLegend), and anti-IL-4-PE (clone 11B11 ...
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bioRxiv - Immunology 2023Quote: ... 4°C) the cells were stained with fluorochrome-conjugated antibodies for FACS Analysis (all antibodies from Biolegend) for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse GL7 (Biolegend, Pacific Blue), IgM (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... ELISA plates were coated with purified antibody (3 µg/ml) overnight at 4°C (BioLegend, catalog 519202). The next day ...
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bioRxiv - Genomics 2024Quote: ... for 10 minutes at 4 °C and then stained with FITC - anti-human EPCAM (BioLegend CAT #32404) and PE - anti-CD45 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... and permeabilized for 20 min at 4℃ with intracellular staining permeabilization wash buffer and fixation buffer (Biolegend). Next ...
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bioRxiv - Bioengineering 2024Quote: ... they were incubated overnight at 4 °C with mouse anti-βIII tubulin (2 µg/mL, TUBB3, Biolegend). After the primary antibody incubation ...
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bioRxiv - Cell Biology 2024Quote: Cells were stained at 4°C for 20 min with lineage markers including CD45-BV421 (Biolegend, 103134), Ter119-BV421 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... IL-17A (capture Ab 5 µgml−1 TC11-18H10.1; detection Ab 2,5µgml−1 TC11-8H4, Biolegend); IFNɣ (capture Ab 5 µgml−1 AN18 ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Immunology 2020Quote: ... supernatant was collected at day 1 and 5 for LEGENDplexTM Human T Helper Cytokine Panel (BioLegend).
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bioRxiv - Cell Biology 2020Quote: ... treated cells were harvested and stained with 5 μg/ml propidium iodide and Annexin V (Biolegend) in Annexin V-binding buffer (10 mM Hepes pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... Th17 cells were differentiated in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-6 (20 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... after which time cells were stained with 5 μg/ml CD8-APC (clone SK1; Biolegend, UK), harvested and CSFE dilution measured by flow cytometry ...
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bioRxiv - Systems Biology 2021Quote: ... centrifuged and resuspended in 5 ml of 1X red blood cell (RBC) lysis buffer (420301, BioLegend) for 5 min on ice ...
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bioRxiv - Immunology 2020Quote: ... Enriched CD4SP thymocytes were then stained with CD4-Pacific blue (RM4-5) fluorochrome-conjugated antibodies (BioLegend). FACS Aria IIu and FACS Aria II SORP (BD Biosciences ...
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bioRxiv - Genetics 2020Quote: Antibodies to the following antigens were used for flow cytometry: CD4 BV650 (RM4-5, #100545, Biolegend), CD8a PE-Cy7 (53-6.7 ...
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bioRxiv - Immunology 2022Quote: ... the cell suspension was incubated in 5 μgml−1 anti-CD16/32 (#101302, Biolegend, 1:200), stained with Live Dead Near IR (#L10119,ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 5 μg/ml anti-hCD3 (BioLegend, Cat# 317326, RRID:AB_11150592), 1 μg/ml anti-hCD28 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... T cells were labeled with 5 µM carboxyfluorescein diacetate succinimidyl (CFSE Cell Division Tracker Kit, Biolegend). For Treg induction and T cell activation ...
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bioRxiv - Cell Biology 2022Quote: ... BV711 Mouse anti mouse CD64 (Monoclonal; clone X54-5/7.1; Biolegend; 1:100; Cat#139311, FACS). BV421 Rat anti mouse CD68 (Monoclonal ...
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bioRxiv - Genomics 2022Quote: ... and extracellular stains with anti-mouse CD4-APC (1:400, clone RM4-5, BioLegend Cat#100516), anti-mouse TCRb chain-FITC (1:400 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were stained for 15 min in the dark with 5 μL APC Annexin V (Biolegend) and 5 μL 7-AAD Viability Staining Solution (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... cells were first stimulated for 5 hours with either anti-CD3 antibodies (1 ug/mL, Biolegend) or MTB300 peptide megapool (21 ...
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bioRxiv - Immunology 2023Quote: ... 5 × 105 NPCs were labeled with viability dye marker (Zombie Aqua, Biolegend, San Diego, CA, USA) and blocked with PBS supplemented with 1% Fc Block (MACS ...
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bioRxiv - Neuroscience 2024Quote: ... Microglia were stained with 5 ul each of PE-anti human CD45 antibody clone HI30 (Biolegend) and APC-anti human CD11b antibody clone ICRF44 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... PBMC were prelabeled with 5 mM Tag-it Violet™ Proliferation and Cell Tracking Dye (Biolegend) in PBS with 0.1% FBS for 10 min in a 37°C water bath ...
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bioRxiv - Developmental Biology 2021Quote: TRA-1-60 staining was performed as previously described (4) and anti-SSEA1 antibody (Biolegend, catalog no. 125604) was used to quantify MEF reprogramming ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with the following antibodies at 4°C for 45 minutes: CD56-BV421 (HCD56, BioLegend), CD4-BV605 (OKT4 ...
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bioRxiv - Immunology 2021Quote: ... for 1 hour and subsequently stained overnight at 4°C with mouse anti-human TCR Vα7.2-PE (Clone 3C10, Biolegend), mouse anti-human CD161-AF488 (Clone NKR-P1A ...
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bioRxiv - Neuroscience 2021Quote: ... single cell suspensions were incubated for 15 minutes at 4°C in Zombie NIR Fixable viability dye (Biolegend) diluted 1:500 in PBS ...
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bioRxiv - Immunology 2021Quote: ... for 15 minutes on ice followed by staining with fluorescently conjugated Abs: anti-CD11a (clone M17/4- Biolegend), anti-KLRG1 (clone MAFA- Biolegend) ...
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bioRxiv - Microbiology 2021Quote: ... primary CD4+T cells were activated for 4 days with 1μg/ml plate-bound αCD3 antibody (cloneOKT3, Biolegend) in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... and then stained for 30 min at 4°C with a FITC-conjugated anti-mouse CD4 Ab (BioLegend) and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)) ...
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bioRxiv - Immunology 2022Quote: ... and then stained for 30 min at 4°C with a FITC-conjugated anti-mouse CD4 Ab (BioLegend) before fixation ...
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bioRxiv - Immunology 2022Quote: ... and then stained for 30 min at 4°C with a FITC-conjugated anti-mouse CD4 Ab (BioLegend) and PE-conjugated anti-mouse CCR5 (a Th1 marker (27)) ...
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bioRxiv - Immunology 2022Quote: ... BioLegend) for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2 ...
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bioRxiv - Immunology 2022Quote: ... for 4 hours, and blocked with secretory inhibitors, brefeldin A (1:1000, eBioscience) and monensin (1:1000, Biolegend) for the last 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... and stained intracellularly for 30 minutes in PBS with antibodies including: PE anti-mouse IL-4 antibody (Biolegend) or PE anti-mouse IFN-γ antibody (Biolegend) ...
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bioRxiv - Developmental Biology 2020Quote: Freshly isolated mammary cells were incubated at 4°C for 20 min with the following antibodies from Biolegend: anti-CD24-BViolet421 (clone M1/69 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (PBS + 4% FBS) supplemented with Fc block (clone 93; BioLegend Cat. 101320) and incubated at 4°C for 5 minutes before staining ...
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bioRxiv - Immunology 2020Quote: ... The following directly conjugated antibodies were used in these experiments: CD4 Alexa Fluor 700 (Clone RM4-4, BioLegend), CD4 BUV737 (Clone GK1.5 ...
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bioRxiv - Cell Biology 2021Quote: Cells dissociated from primary HMEC strains (passage 4) were stained with anti-human CD271-PerCP/Cy5.5 (Biolegend #345122) and anti-human CD133-PE (Biolegend #372804 ...