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Citations for BioLegend :
851 - 900 of 1849 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... For intracellular staining cells were either permeabilized with 0.5% Saponin or 0.1% Triton and stained for TNF-α and/or IL-6 (BioLegend). Cells were washed with 2% serum supplemented 1X PBS and subsequently fixed with 1% PFA ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2022Quote: ... male mice were isolated 6 dpi with NP-OVA/alum and stained with anti-CD4 Alexa Fluor 647 (GK1.5, BioLegend), anti-TCRβ PE-Cy7 (H57-597 ...
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed in 3.7% formaldehyde for 6 min and PM HA-GLUT4-GFP detected by an anti-HA antibody (901503, BioLegend). HA staining was visualized by Cy3-goat anti-mouse secondary antibody (115-165-062 ...
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bioRxiv - Immunology 2023Quote: ... both endometriosis and sham mice were treated with intraperitoneal (i.p.) injections of 1μg of rmIL-23 (n=6/group; 589006, BioLegend, USA), or PBS for controls (n=6/group) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cat #126404), and PE anti-mouse H2-Kb/H2Db antibody (28-8-6, cat#114607) were purchased from BioLegend. Anti-VHL (VHL40 ...
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Lipid Nanoparticles from L. meyenii Walp Mitigate Sepsis through Multimodal Protein Corona FormationbioRxiv - Bioengineering 2024Quote: ... Then the supernatant was collected to measure IL-6 and TNF-α levels using ELISA (Biolegend, San Diego, CA).
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bioRxiv - Bioengineering 2024Quote: ... streptavidin-conjugated MBs were washed once by centrifugation and incubated with 6 µg biotin anti-human αvβ3 antibody (304412, BioLegend) on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: ... fixed with 4% paraformaldehyde fixation buffer (BioLegend 420801, San Diego, CA, USA) for 20 minutes at room temperature and washed twice more with MACS buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated at 4°C overnight with ATF4 antibody (BioLegend) at 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... for surface staining and BV605-anti-IL-4 (504125, BioLegend Europe BV), APC-anti-IL-5 (504306 ...
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bioRxiv - Immunology 2022Quote: ... cells were fixed for 20 min with 4% paraformaldehyde (Fixation buffer; BioLegend). When intracellular staining was performed ...
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bioRxiv - Neuroscience 2021Quote: ... and CD284 (TLR-4, clone SA15-21, PE, 2.5 µg/ml Biolegend). After staining ...
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Sterilizing immunity against SARS-CoV-2 in hamsters conferred by a novel recombinant subunit vaccinebioRxiv - Microbiology 2020Quote: ... and further stained with BV421-conjugated anti-mouse IL-4 (Biolegend, 504127) and ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4 °C with Zombie Violet staining (BioLegend, 423113) to exclude dead cells ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% paraformaldhyde and permeabilised with CytoPerm buffer (Biolegend). Cells were analysed on BD LSR Fortessa X-20 cytometer ...
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bioRxiv - Cancer Biology 2020Quote: ... rotating at 4°C with either μ5g IRF3 antibody (BioLegend, clone:12A4A35) or equivalent isotype control conjugated with protein A/G magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: TruStain FcX (Biolegend #422302), CD45-AF647 (clone QA17A19 ...
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bioRxiv - Immunology 2024Quote: ... CD4 (clone RM4-4) and CD8 (clone 53-6.7) were from Biolegend. Cleaved caspase 3-AF647 (Clone D3E9 ...
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bioRxiv - Immunology 2024Quote: ... and 10 μg/mL anti-mouse IL-4 antibody (clone 11B11, BioLegend)) or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304 ...
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bioRxiv - Immunology 2023Quote: ... and 10 µg/mL anti-IL-4 (BioLegend, Cat no. 504-102) to achieve Th1 polarization ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained (20 min, 4°C) with B220-PerCP/Cyanine5.5 (#103235; BioLegend) and CD138-PE (#142504 ...
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bioRxiv - Cancer Biology 2023Quote: ... PerCP/Cyanine5.5 anti-mouse CD4 Antibody (BioLegend, catalog #116011, clone RM4-4), PE/Cyanine7 anti-mouse CD8α Antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: TruStain FcX (Biolegend #422302), CD45-BV421 (clone HI30 ...
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bioRxiv - Immunology 2024Quote: ... Standards for both IL-4 and IL-13 were purchased from BioLegend. IL-10 ELISA kit was purchased from BioLegend.
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bioRxiv - Bioengineering 2024Quote: ... or (donors 3-4) anti-CD19 AF488 (BioLegend catalog #302219, clone HIB19), anti-CD3 PerCP/Cy5.5 (TONBO catalog #65-0037-T100 ...
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bioRxiv - Immunology 2024Quote: ... Cells were first stained at 4° C in Cell Staining Buffer (BioLegend) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... anti-human CD27 (BioLegend, Clone M-T271, PE-Dazzle, 1:100, 4℃); anti-human IgD (BioLegend ...
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bioRxiv - Immunology 2022Quote: T2 cells were stained with 5 μM Tag-It Violet (BioLegend) following the manufacturer’s protocol and then 60000 cells were seeded in a volume of 100 μL per well in a V-bottom 96 well tissue culture plate ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with 5 μL of APC-Annexin V (Biolegend) in 45 μL of 1x Annexin V binding buffer (10 mM Hepes (pH 7.4) ...
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bioRxiv - Systems Biology 2021Quote: ... cells were stained with a keratin 5 polyclonal antibody (BioLegend #905501) at 1:800 overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: After the addition of 5 ul Human TruStain FcX™ (BioLegend), the cell suspension was incubated for 10 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... 5’ tetramethyl benzidine (TMB) substrate and peroxidase (Biolegend, San Diego, CA) at room temperature for 7.5 min before adding 2.5 N H2SO4 was quantified by Bioanalyzer at 450nm ...
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bioRxiv - Microbiology 2021Quote: ... We stained platelets with 5 µL Annexin V (BioLegend, Koblenz, Germany) in Annexin V binding buffer (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Primary surface staining included: anti-mouse CD4 (clone RM4-5, BioLegend), CD8a (clone 53-6.7 ...
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bioRxiv - Immunology 2022Quote: ... Cells were stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... cells were stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... with 5 ul of Fc receptor Block (TrueStain FcX, Biolegend, USA) for 15 min ...
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bioRxiv - Cancer Biology 2020Quote: ... and polyclonal chicken anti-Keratin 5 (Biolegend, Cat: 905901, 1:100). Subsequently ...
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bioRxiv - Neuroscience 2020Quote: ... followed by the incubation with 5 µg/ml DAPI (Biolegend, 422801), eBioscience DRAQ5 (Thermofisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and 5 µl True Stain Monocyte blocker (Biolegend, Catalog no 426101) was added in every tube ...
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bioRxiv - Physiology 2020Quote: ... by utilizing anti-HA antibody (5 µl/ sample; Cat#901513, Biolegend).
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bioRxiv - Immunology 2020Quote: ... anti-Ly6C (HK 1.4) and anti-D64 (X54-5/7.1, BioLegend), anti-SiglecF (E50-2440 ...
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bioRxiv - Immunology 2020Quote: ... CD4-Alexa Fluor 488 (clone: RM4-5; dilution 1:400, BioLegend), CD8a-APC/Cy7 (clone ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μL of Abs-Aβ (5-75 μg/mL, 6E10 BioLegend) was added to a suspension of gold nanorods at a concentration of 5 nM in 10 mM MES buffer at pH 6 ...