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Citations for BioLegend :
751 - 800 of 1063 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Biotinylated pneumococcal serotype 3 polysaccharide with a 5% biotin load was coupled with high concentration streptavidin-phycoerythrin (SA-PE) and streptavidin-allophycocyanin (SA-APC, both from BioLegend, San Diego, CA) in separate reactions ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant proteins Serpin E2 (BioLegend, 769002) and Serpin A3 (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Genomics 2019Quote: ... The resuspended cells were incubated for 20 min at 4 °C with the fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Immunology 2021Quote: ... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
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bioRxiv - Immunology 2021Quote: The following antibody mixture was made and used to stain the flow through and labeled fractions: B220-BV510 (Biolegend) at 1:20 ...
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bioRxiv - Cancer Biology 2020Quote: ... we collected the beads and labeled them with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated with A32-APC (3.3 μg/ml) for 25 minutes and labeled with anti-CD3-AF700 (SK7, Biolegend) and anti-CD32-PE-Cy7 (FUN-2 ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat ...
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bioRxiv - Immunology 2021Quote: ... counted and up to 1×108 cells were labeled with 1.5-2 μM Carboxyfluorescein succinimidyl ester (CFSE; BioLegend, USA) in 1 mL PBS 1X for 20 min according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were washed and incubated with fluorescently-labeled antibodies (B220 V500, FAS FITC, CD38 Alexa fluor 700; Biolegend) for 20-40 min ...
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bioRxiv - Immunology 2019Quote: ... OVA presentation was confirmed by flow cytometry using a PE-labeled antibody against surface SIINFEKL bound to H-2Kb (clone 25-D1.16, BioLegend).
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bioRxiv - Immunology 2020Quote: ... OVA presentation was confirmed by flow cytometry using a PE-labeled antibody against surface SIINFEKL bound to H-2Kb (clone 25-D1.16, BioLegend).
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bioRxiv - Systems Biology 2021Quote: ... and then stained the cells for 30 min with 1:500 anti-NGFR APC-labeled clone ME20.4 (BioLegend, 345107). We washed the cells five times with 0.1% BSA/PBS and followed with one final wash with PBS for 2 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Stem and progenitor cells were isolated using fluorescently labeled or biotinylated antibodies for the following antigens: cKit (2B8, Biolegend), Sca1 (D7 ...
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bioRxiv - Immunology 2022Quote: ... MR1 surface expression was evaluated by flow cytometry with APC-labeled mouse anti-MR1 mAbs 26.5 (Biolegend, Cat#361108) and APC-labeled mouse IgG2a (clone MOPC-173 Biolegend ...
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bioRxiv - Immunology 2022Quote: ... and stained with a cocktail of fluorescently labeled anti-cytokine antibodies: IFN-γ PE/Dazzle 594 (Biolegend, cat.505845), TNF-α PE/Cyanine7 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions from individual tumors were then labeled with hashtag oligonucleotides following the manufacturer’s protocol (TotalSeq B0301-B0306, Biolegend). Each individual tumor sample was counted and then pooled at an equal cell ratio before being split into two replicates for library preparation with the Chromium Single Cell 3’ V3 (10X Genomics ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45highCD8+ cells were labeled with rat anti-CD45 APC (1:100, catalog no. 103111; BioLegend), rat anti-CD8 PerCP/Cyanine5.5 (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and labeled with propidium iodide (PI) and Annexin-V-FITC (Annexin V FITC Apoptosis Detection Kit with PI, Biolegend) for the detection of necrosis and apoptosis ...
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bioRxiv - Immunology 2024Quote: ... Then the cells were labeled as described above using antibodies anti-CD3-BV421 (clone 17A2) (BioLegend, SanDiego, CA, USA) (1:200 ...
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bioRxiv - Immunology 2023Quote: ... cells treated with a Foxp3/Transcription factor staining buffer kit (Cat. TNB-0607, TOMBO Bioscience) were stained with FITC-labeled anti-mouse CD4 Ab (GK1.5, BioLegend) and allophycocyanin-labeled anti-mouse Foxp3 Ab (3G3 ...
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bioRxiv - Synthetic Biology 2024Quote: ... as previously described [63] and neutrophils were labelled by were labeled with 2.5 µg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit ...
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bioRxiv - Cell Biology 2024Quote: ... cells were labeled with antibodies detecting the following cell-surface markers: CD133-APC (Biolegend, clone 7, dilution 1:20), CD19-PECy7 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Enriched samples were then stained on ice for 30 minutes with the following antibodies: BV421-labeled CD45.1 (A20, BioLegend), BV785-labeled CD45.2 (104 ...
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bioRxiv - Immunology 2024Quote: ... to lyse the red blood cells and then stained on ice for 30 minutes with the following antibodies: BV421-labeled CD45.1 (A20, BioLegend), BV785-labeled CD45.2 (104 ...
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bioRxiv - Bioengineering 2024Quote: ... and stained with fluorescently-labeled antibodies (i.e., PE/Cy7-CD11c, APC-MHC II, PE-CD86, and FITC-CD40 – Biolegend) for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Cells from each sample were labeled with TotalSeq™-B0251-0254 anti-human Hashtag Antibodies (clone LNH-94, Biolegend, RRID ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated for 20 min at room temperature in 100 μl of FACS buffer (0.1% FBS in PBS) containing fluorochrome-labeled antibodies (BioLegend) against human CD3 (Pacific blue ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and incubated with fluorescently labeled antibodies (CD4 PE, clone RM4-5, 1:400, BioLegend; CD8a FITC, clone 53-6.7, 1:400, BioLegend; B220 PE-Cy7 ...
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bioRxiv - Bioengineering 2021Quote: ... IL-6 was quantified using ELISA developed in the laboratory using biotin anti-rat IL-6 antibody (cat. # 517703) and purified (coating) anti-rat IL-6 antibody (cat. # 517701, Biolegend, San Diego, CA, USA). Coating antibody was diluted in 100 mM NaHCO3 ...
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bioRxiv - Cancer Biology 2021Quote: ... The samples were blocked with 0.5 % BSA for 10 min on ice and incubated with the primary mAbs biotin-anti-human CD274 (B7-H1, PD-L1) (clone 29E.2A3, BioLegend, San Diego, CA, USA) or biotin anti-mouse CD274 (clone 10F.9G2 ...
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bioRxiv - Bioengineering 2021Quote: ... IL6 was quantified using an ELISA developed from an antibody pair (biotin anti-rat IL6 detection antibody, cat. #517703, and purified anti-rat IL6 coating antibody, cat. #517701, Biolegend, San Diego, CA, USA). For this ELISA ...
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bioRxiv - Synthetic Biology 2021Quote: ... The knock-out of the T cell receptor (TCR) was assessed with the absence of signal after staining 1:200 with APC-labeled anti-CD3ε (Biolegend). Each CAR construct contained a Strep tag which allowed for a two-step staining to validate successful knock-in ...
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bioRxiv - Immunology 2021Quote: FACS purified cell subsets were labeled by incubation for 30 minutes at 4°C with TotalSeq anti-mouse Hashtag antibodies (BioLegend), then washed ...
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bioRxiv - Immunology 2021Quote: ... Cells were labeled with live/dead dye (Zombie Aqua) for 10 min at RT and blocked with human TruStain FcX (BioLegend) for 20 min at RT ...
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bioRxiv - Microbiology 2021Quote: ... Cells were labeled with the following antibodies directly conjugated to the following fluorophores: CD45-PE/Cy7 (clone 30-F11, BioLegend), CD11b-BV510 (clone M1/70 ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD71 expression in TF-1 and DAMI cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD71 (BioLegend) (#113811) ...
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bioRxiv - Cancer Biology 2021Quote: ... For the assessment of CD11b expression MDS92 and MDS-L cells were labeled with the phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated CD11b (BioLegend) (#557743) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following anti-mouse antibodies we used for surface staining: Alexa Fluor 647-labeled H-2Kb/H-2Db – MHC Class I (clone 28-8-6, Biolegend); PE-labeled I-A/I-E – MHC Class II (Clone M5/114.15.2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL Binding Buffer containing 100 nM M1 anti-FLAG antibody labeled with Alexa Fluor 488 and Alexa Fluor 647 anti-human IgG Fc (BioLegend) diluted 1:100 (v/v) ...
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bioRxiv - Biophysics 2022Quote: ... were established by transfection of pIRES-HA-CXCR4 and pIRES-HA-CXCR4-W1955.34L and subsequent cell sorting for equivalent surface expression levels using 1:1000 Alexa Fluor 488-labeled anti-HA antibody (clone 16B12, Biolegend). U87 cells were grown in Dulbecco’s modified Eagle medium (Themo Fischer Scientific ...
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bioRxiv - Immunology 2022Quote: ... The nanoparticles were washed again and finally resuspended in the 100 µL of assay buffer containing fluorescently labeled AlexaFlora568-detection antibody for IFNγ (Biolegend).
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat: 352911; anti-HER2-BV421, Biolegend, Cat ...
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bioRxiv - Immunology 2020Quote: Recipient mice were infused with 2-3 x 106 purified donor T cells that were labeled with 5 μM CFSE (BioLegend) as previously described63 ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25high Treg cells were enriched from PBMCs after staining with PE-cyanine 5 (PE-Cy5)-labeled anti-CD25 Ab (BioLegend), followed by positive selection using anti-PE and anti-Cy5 microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: Alexa fluor 488 or Alexa fluor 594 or FITC or Alexa fluor 648 labeled anti-His-tag (MBL or Biolegend),
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bioRxiv - Immunology 2022Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...
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bioRxiv - Immunology 2019Quote: ... cells were washed once in in 1ml of PBS-1%FCS (8’, 350rcf).Cells were then separately labeled with hashtag antibodies (Biolegend TotalSeq-A antibodies ...
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bioRxiv - Immunology 2021Quote: ... phagocytosis was stopped by washing with 4°C PBS and centrifugation at 1200 RPMI before the cells were stained with APC-labeled anti-CD11b (Clone M1/70, Biolegend) to identify human macrophages and Live/Dead stain (Fixable Viability Dye ...