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Citations for BioLegend :
601 - 650 of 1018 citations for Myelin Basic Protein Biotin labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Primary mouse lung fibroblasts were freshly isolated after magnetic bead-based negative selection of epithelial cells (118204, Biotin anti-mouse CD326 Epcam, Clone: G8.8, BioLegend), endothelial cells (102404 ...
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bioRxiv - Immunology 2024Quote: ... cells were removed by washing and the plates were washed and probed with biotin α-IFNγ (clone XMG1.2, Biolegend) followed by incubation with Streptavidin-ALP (Mabtech) ...
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bioRxiv - Immunology 2019Quote: ... Sections were labeled overnight at 4°C with mouse anti-human CD1c-PE (L161, Biolegend) or mouse anti-human Clec9A-PE (8F9 ...
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bioRxiv - Immunology 2021Quote: ... and then fluorochrome-labeled antibodies to surface markers and secondary antibody against rabbit IgG (Biolegend) were added ...
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bioRxiv - Microbiology 2021Quote: ... samples were resuspended in M199s medium and labeled using Zombie Violet Fixable Viability dye (BioLegend), prepared according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: PerCP-CyTM5.5 or FITC-labeled Annexin V were obtained from BioLegend (catalog #640936 and 640905). As specified by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with PE labeled anti-human IgG Fc secondary antibody (Biolegend) at a 1:20 dilution in 50 μl staining buffer at room temperature for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... MCM-treatment vs CTRL) were labeled with Cell Hashing antibodies following the manufacturer’s instruction (BioLegend), cells were counted on Countess II automated cell counter (Thermo Fisher ...
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bioRxiv - Immunology 2019Quote: ... and macrophages were labeled with anti-F4/80 (BV421) (Biolegend Clone: BM8 | Dilution: 1:100). Data were acquired on ImageStreamXMKII (Amnis ...
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bioRxiv - Cancer Biology 2021Quote: Circulating immune cells were labeled using intravenously injected APC-conjugated CD45 antibody (30-F11, Biolegend) 3 minutes prior to sacrifice as previously described (39,93).
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bioRxiv - Immunology 2022Quote: ... 5×105 SABR expressing NFAT-GFP-Jurkat cells were labeled with cell trace violet (BioLegend) according to the manufacturer’s protocol before incubation with 5×105 TCR expressing Jurkat cells in a round bottom 96-well plate for 16-20 hr ...
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bioRxiv - Immunology 2022Quote: ... Omicron RBD (BA.2 or BA.5) were labeled with TotalSeq-C0971 Streptavidin (Biolegend, 405271) and TotalSeq-C0972 Streptavidin (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Nalm6 and primary B cells were labeled with PE/Cy7 anti-human CD19 Antibody (Biolegend) and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2 ...
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bioRxiv - Immunology 2022Quote: ... Enriched cells from naïve mice were stained with Pacific Blue-labeled B220 (RA3-6B2, BioLegend), Pacific Blue-labeled CD90.2 (53-2.1 ...
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bioRxiv - Immunology 2022Quote: ... in vitro-differentiated cells were labeled with the following antibodies: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Stimulation was performed in the presence of fluorophore-labeled anti-CD107a mAb (clone 1D4B; Biolegend) or the isotype-matched mAb control ...
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bioRxiv - Microbiology 2023Quote: ... we administered 2 μg of APC/Fire750-labeled anti-CD45 mAb (Biolegend, clone 30-F11) in mice anesthetized with an overdose of ketamine and xylazine ...
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bioRxiv - Immunology 2023Quote: ... add intracellular direct-labeled antibodies: PE anti-mouse IRF5 (clone: W16007B, cat. no. 158603, BioLegend) and Alexa Fluor 647 anti-IRF4(clone ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were stained with fluorescein isothiocyanate (FITC) labeled HLA–DR antibody (Biolegend, Cat. No. 307604) in FACS buffer for 1 hour at 4°C and analyzed by flow cytometry.
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bioRxiv - Immunology 2024Quote: ... and fluorochrome-labeled antibodies anti-CD3-PE and anti-CD4- or anti-CD8-FITC (BioLegend) either in FACS buffer (PBSO ...
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bioRxiv - Immunology 2024Quote: ... B16-ova tumors were i.v labeled with CD8α-Alexa 488 (Clone 53-6.7, BioLegend 100723). Cells were blocked for 10 minutes with Fc block (DartLab Immune Monitoring Core) ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with 25 µg of Alexa Fluor-647 labeled CD31 antibody (MEC13.3, BioLegend) retro-orbitally ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: Cells were incubated with anti-CD16/32 (Fc block) prior to staining with the following fluorophore or biotin conjugated monoclonal antibodies purchased from Biolegend, eBioscience ...
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bioRxiv - Genetics 2021Quote: ... we negatively isolated memory T cells with a modified Pan T cell magnetic-activated cell sorting (MACSR) kit with anti-CD45RA biotin and followed an optimized version of the CITE-seq protocol with TotalSeqTM-A (BioLegend) oligonucleotide-labeled antibodies for a panel of 31 surface proteins (43) ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Immunology 2020Quote: ... or biotin, 2G5, Beckman Coulter, or Ber-ACT8, Brilliant Violet 711, BUV395, BD Biosciences, or Ber-ACT8, PE-Cy-7, Biolegend), KIR2DL1 (FAB1844F ...
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Immunology 2019Quote: ... to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience, Biolegend, Thermofisher Scientific or Miltenyi biotec ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with a 1:100 dilution of biotin anti-human IgG Fc antibody (clone HP6017, BioLegend) for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088 ...
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bioRxiv - Cell Biology 2022Quote: ... Erythroid precursors were isolated using negative selection by lineage-depletion with biotin- conjugated antibodies and MojoSort streptavidin-conjugated magnetic nanobeads (Biolegend): Anti-mouse CD3e (clone 145-2C11) ...
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bioRxiv - Immunology 2021Quote: ... splenocytes from immunized mice were stained with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin followed by 1 μg/mL of streptavidin (BioLegend) at 4°C ...
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bioRxiv - Immunology 2020Quote: ... ABCs were isolated from purified total B cells by CD11c positive selection using a biotin conjugated CD11c antibody (301612, Biolegend) and anti-biotin magnetic beads ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (1% BSA in PBS) at a concentration of 108 cells/mL and 1 μL negative CD8+ T cell biotin-antibody cocktail (Biolegend)/million cells was added and allowed to incubate for 15 min on ice ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and at passage 1 a depletion of CD45+ cells was performed using a Biotin anti-mouse CD45 Antibody (Biolegend, 103104) and Dynabeads™ Biotin Binder (ThermoFisher 11047) ...
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bioRxiv - Immunology 2023Quote: ... CD8 (biotin, clone: 53-6.7), CD205 (clone: NLDC-145) and TNF-α(PE, clone: MP6-XT22) were purchased from BioLegend. The CD18-reactive mAb M18/2 was purchased from Biozym ...
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bioRxiv - Immunology 2023Quote: ... and GM-CSF (purified anti-mouse GM-CSF; biotin anti-mouse GM-CSF) ELISA antibodies and standards were purchased separately from Biolegend.
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bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
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bioRxiv - Genomics 2023Quote: ... The cells were then incubated on ice for another 30 min after adding 0.5 μl of biotin anti-human CD15 antibody (BioLegend, 301913). After immunostaining ...
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bioRxiv - Immunology 2023Quote: ... the following antibodies were used at a 1:200 ratio: epithelial cells (324216, Biotin anti-human Epcam, clone:9C4, BioLegend), endothelial cells (13-0319-82 ...
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bioRxiv - Cancer Biology 2023Quote: ... non-natural killer cells were depleted by incubating the patient’s whole PBMCs sample with the biotin antibody cocktail (BioLegend 480053) followed by incubation with magnetic Streptavidin Nanobeads ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in 200 uL PBS containing 2% FBS and Fc block and incubated with biotin anti-mouse F4/80 (Clone BM8, BioLegend), CD3 (Clone 500A2 ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... cells were permeabilized and labeled with the described cocktail plus APC-IFN-γ (BioLegend Cat# 505810) and PE-IL-17A (BioLegend Cat# 506903) ...
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bioRxiv - Molecular Biology 2020Quote: ... the UCB- and PB-MNCs were labeled with PE-conjugated anti-human CD34 (Biolegend, Cat.No.343506), APC-conjugated anti-human CD133 (Miltenyibiotec ...
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bioRxiv - Immunology 2019Quote: Neutrophils obtained after 4-day differentiation (“in vitro-derived”) were washed and labeled using CFSE (BioLegend). Murine bone marrow neutrophils were isolated by negative selection (StemCell Technologies ...
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bioRxiv - Cell Biology 2019Quote: THP-1 cells were labeled with mouse antibody anti-human CD18 (PE BioLegend or BV421 BD) (1 µl per 200 000 cells ...
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bioRxiv - Bioengineering 2021Quote: ... the positive target cells (Raji) and negative target cells (K562) were labeled with Calcein-AM (Biolegend) and then cocultured with effector cells (CAR19 and sCAR19 cells ...
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bioRxiv - Immunology 2020Quote: Flow cytometry analysis was performed with fluorescently-labeled monoclonal antibodies to mouse CD4-APC (GK1.5, BioLegend) or CD8a-PE (53-6.7 ...