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Citations for BioLegend :
7801 - 7850 of 7947 citations for Rat Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Tumor single-cell suspensions were stained with Alexa Fluor 700 anti-CD326 (Ep-CAM) antibody (Biolegend, 118239, clone: G8.8, 1:100 dilution), PE/Cyanine 7 anti-mouse CD31antibody (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... then were resuspended in FACS (PBS + 3% FBS + 1mM EDTA + 10mM HEPES) supplemented with TruStain FcX (1:50; BioLegend; San Diego, CA) and TruStain Monocyte Blocker (1:20 ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of PRP was incubated with 1 μg/mL AF647-conjugated CD42b rabbit anti-human antibody (BioLegend, San Diego, CA, USA) to label the platelets ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
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bioRxiv - Immunology 2022Quote: ... CD3ε clone 145-2C11; Ly-6G/Ly-6C clone RB6-8C5; CD11b clone M1/70; CD45R/B220 clone RA3-6B2; TER-119, Biolegend, 1/200). The c-kit− fraction was stained with antibodies against CD11b (PercPCy5.5 ...
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were resuspended in 0.1% Tween-20 in PBS supplemented with 5% BSA at the following dilutions: TUJ1 (BioLegend, 801202, 1:400), SOX2 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 90 μl of 1% BSA in PBS and Fc blocked with 10 μl Human Trustain FcX (Biolegend cat. 422302) for 10 minutes on ice then stained on ice for 45 minutes with a pool of 268 antibodies in 100 μl ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS and stained at 4°C with 100 µL 1:500 Zombie Aqua viability dye (BioLegend, Cat# 423102) and 1:200 mouse FC block (TruStain FcX ...
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bioRxiv - Immunology 2023Quote: ... IL-1b, IL-6, IL-10, IL-17A, IL-12p70, GM-CSF, IL-23, IFN-b, MCP-1, IL-27; BioLegend Cat# 740446), Mouse TH Panel v4 (TNF-α ...
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bioRxiv - Bioengineering 2023Quote: ... The following antibodies were mixed together at 2x concentration in 50 μL and added directly to the cell suspension: BV605 CD45 (1:500, Biolegend, cat#103139), AF700 CD11b (1:300 ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using antigen probe (1.5 μL/well) and the following conjugated antibodies: APC anti-human CD3 (300412, BioLegend; 1 μL/well), PerCP/Cyanine 5.5 anti-human CD19 (302230 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were immerged in a 100% formic acid for 10 min followed by a brief wash in DI H2O and PBS and an incubation with anti-Ab (800701, Biolegend – 1/500) for 24h at 4°C ...
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bioRxiv - Microbiology 2024Quote: Suspensions of ∼106 HIV-infected TMCs in PBS with 1% FBS were incubated with BV711 anti-human CD3 antibody (Biolegend, catalog # 300464) at 4°C for 30 min ...
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bioRxiv - Bioengineering 2024Quote: Supernatants were collected after a 48-hour PBMCs and TCM incubation and immediately assessed for cytokine secretion using LEGENDplex Human Inflammation Panel 1 (Biolegend catalog #740809) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... cells were sensitized with mouse IgG2a cell-type specific antibodies (HUVEC and THP-1: CD31, HEC7, Invitrogen, 2 μg; Fibroblasts: HLA-A,B,C, W6/32, Biolegend, 2 μg) or a mouse IgG2a isotype control (MG2a-53 ...
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bioRxiv - Neuroscience 2024Quote: ... Fixation and cryostat sectioning of 3D organoids was performed as previously described.47 Immunostaining was done using antibodies to PAX6 (BioLegend, 1:200), SOX2 (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: Isolated cells were stained at 4°C in FACS buffer (PBS supplemented with 2.5% bovine serum albumin) with and hematopoietic lineage markers including Ly6G (BioLegend, clone 1A8, 1:600), CD11b (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellets were washed once with 15 mL PBS and resuspended in 1 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 2 min at room temperature to lyse RBCs ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with FACs buffer (1X PBS, 1% FBS) and fixed at room temperature for 20 minutes with Intracellular Staining Fixation Buffer (BioLegend, San Diego, CA). Cells were washed with 1X Cyto-Fast™ Perm Wash solution (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... The stain solution contained a 1:200 dilution of an antibody against H-2Kb bound to SIINFEKL conjugated to PE (#141603, Biolegend, San Diego, CA) to detect SIINFEKL peptide being presented at the cell surface ...
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bioRxiv - Cancer Biology 2019Quote: ... or U2S3-hFc-mutIL15 for two hours before fixing and staining for intracellular phospo-STAT3 (Biolegend PE anti-STAT3 Tyr705 clone 13A3-1) and –STAT5 (BD Alexa Fluor 647 anti-STAT5 pY694 clone 47) ...
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bioRxiv - Microbiology 2021Quote: ... 20 μl cells were removed for flow analysis and remaining cells were incubated for 30 min on ice with 1 μg each of TotalSeq™-A0441 anti-mouse CD71 Antibody (Biolegend, Cat# 113824) and TotalSeq™-A0073 anti-mouse/human CD44 Antibody (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... 50-μm brain sections were blocked with normal goat serum for 2 h and incubated overnight using biotin-conjugated anti-HA (Biolegend 901505, 1:500) and anti-parvalbumin (Synaptic Systems 195 004 ...
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bioRxiv - Biochemistry 2020Quote: ... Traditional antibodies based labeling procedure was done by using c-Myc Antibody (9E10, Cat # 626801, BioLegend, USA; incubation 1 h at 4°C) and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143 ...
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bioRxiv - Neuroscience 2020Quote: ... Axons were identified using a staining against the phosphorylated neurofilaments (SMI31) stained with the PAB mouse-anti-SMI31 (Biolegend, cat. 801601, 1:250) and the SAB GAM-STAR580 (Abberior ...
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bioRxiv - Cell Biology 2021Quote: ... Immunostaining was performed with biotinylated monoclonal mouse antitubulin (clone 236-10501, Thermo, 1 μg/ml) then Brilliant Violet 421-conjugated streptavidin (Biolegend, 0.1 μg/ml), Alexa Fluor 488-conjugated mouse anti-perforin (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... 50μM coronal sections containing dorsal hippocampus were blocked in normal goat serum for 2 h and incubated overnight using a biotin conjugated anti-HA (Biolegend 901505, 1:500), anti-pS6 244-247 (ThermoFisher 44-923G ...
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bioRxiv - Neuroscience 2020Quote: ... Aβ peptides were extracted from 250 µL human plasma using immunoprecipitation with anti-β-Amyloid 17-24 (4G8) and anti-β-Amyloid 1-16 antibodies (6E10, both Biolegend®) coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads (Thermofisher ...
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bioRxiv - Neuroscience 2022Quote: ... Splenocytes were then filtered through a 40μM filter and stained with an antibody cocktail [Pacific Blue anti-CD45.1 (Biolegend no. 110722; 1:100 dilution), APC anti-CD45.2 (Biolegend no ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Immunology 2022Quote: ... extracellular staining was done in ice-cold buffer (phenol red–free DMEM, 2% FBS) for 30 min using antibodies against CD45 (30-F11; BioLegend, dilution 1:200), CD4 (RM4-5 ...
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bioRxiv - Microbiology 2022Quote: ... single cell suspensions from mouse spleens were stained with fluorescein isothiocyanate (FITC)-conjugated anti-Ly-6A/E (Sca-1) mAb (Biolegend; San Diego, CA). To analyze erythrocyte maturation ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed in PBS/1% BSA buffer and incubated for 30 minutes on ice with the following antibodies from Biolegend (San Diego, USA): FITC-anti-human CD38 (#303503) ...
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bioRxiv - Neuroscience 2020Quote: ... and were stained at 20 μl of FACS buffer per 1×106 cells containing the first set of following antibodies (all from Biolegend, unless specified otherwise): biotinylated hematopoietic lineage antibodies against NK1.1 (clone PK136) ...
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bioRxiv - Immunology 2020Quote: A total of 1×106 splenocytes or liver lymphocytes were surface stained with Brilliant Violet (BV) 421-conjugated anti-CD107a (clone 1D4B, BioLegend, San Diego, CA) during the 24 h stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... and the nerve sections were incubated for 24 hours at room temperature with gentle rocking: mouse anti-TUJ1 for microtubules in the axons (1:1000, Biolegend Catalog No. 801202), rat anti-PECAM-1 for endothelial cells (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Developmental Biology 2022Quote: ... MGs for whole mount samples and cryosections were stained with a rabbit anti-keratin 14 (K14) polyclonal antibody (1:1000, 905301, BioLegend, San Diego, CA). MG whole mounts and cryosections were imaged with a Zeiss LSM880 confocal microscope ...
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bioRxiv - Immunology 2019Quote: ... cells were washed with 200 μl FACS Buffer and blocked in 100 μl Fc block (cat. no. 101319, TruStain FcX, clone 93, BioLegend, diluted 1:100) in FACS Buffer for 10 min on ice and washed ...
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bioRxiv - Systems Biology 2019Quote: ... We washed the samples again with 0.1% BSA-PBS and then incubated for 10 minutes at 4°C with 1:250 anti-NGFR antibody conjugated directly to PE-Cy7 (Biolegend, 400126, clone ME20.4) in 0.5% BSA-PBS with 2mM EDTA ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly harvested PDX or EHS tumors were processed to single cells47 and stained with PE-conjugated anti-human CD298 PE (BioLegend; 1:50 dilution) and FITC-conjugated anti-mouse CD45 FITC (BioLegend ...
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bioRxiv - Neuroscience 2019Quote: ... The triangularis sterni muscle was dissected and incubated overnight (4°C) in anti-βIII-tubulin antibody conjugated to Alexa488 (BioLegend 801203; 1:200) and Alexa594-conjugated α-bungarotoxin (Invitrogen B13423 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... Cells with successful PD-L1 deletion were isolated by two rounds of FACS sorting after O/N stimulation with 100 ng/ml IFNγ and staining with PD-L1-PE/Cy7 (clone 10F.9G2, Biolegend 124314, 1:200). Cells retaining PD-L1 expression were isolated simultaneously and served as controls.
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with Fc-blocking anti-CD16/32 anti-bodies followed by cell hashing antibody (1 μg/ml, BioLegend Cell Hashing A) (Stoeckius et al. ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACS buffer followed by PBS wash and stained with 100 μl of 1:1000 dilution of fixable Zombie Aqua (BioLegend, San Diego, CA) dye for 20 minutes at 4°C in dark ...