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Citations for BioLegend :
7551 - 7600 of 7947 citations for Rat Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... and incubated for 30 minutes on ice with the following antibody: anti-CD44 APC/Fire 750 (1:100, Biolegend, 103061), anti-CD24 PE (1:20 ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA in HBSS) at 1 x 107 cells/mL and incubated with Fc block (anti-mouse CD16/32; clone 93. BioLegend) for 10 minutes before incubation with specific antibodies listed in Table S1 ...
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bioRxiv - Immunology 2023Quote: Peyer’s patch leukocytes from 10 WT B6 mice de novo colonized for 21 days with SFB were separately stained with oligo-hashtagged antibodies (TotalSeqC 1 – 10, Biolegend), prior to pooling ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies and dilutions used for western blot were: Mouse Anti-Aβ (6E10 clone, 1:1,000, Biolegend, Cat# 803001, RRID:AB_2564653), goat anti-Mouse DKK-3 (1:1,000 ...
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bioRxiv - Immunology 2023Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer preincubated with streptavidin (30 min at molar ratio of 1:4 at 25°C) conjugated to phycoerythrin (405203, BioLegend) and/or BV421 (405226 ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Immunology 2023Quote: VLP-producing Expi293F cells were extracellularly and intracellularly stained with Allophycocyanin (APC) anti-Flag (DYKDDDDK) tag antibody (1:500) (Biolegend) and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24 ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Genomics 2023Quote: ... A subset of the hiPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained for sgRNA expression with an APC conjugated anti-mouse CD90.1/Thy1.1 antibody (no. 202526, BioLegend; 1:400) and Human TruStain FcX Fc receptor blocking solution (no ...
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bioRxiv - Genomics 2023Quote: ... Many of the iPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Plant Biology 2024Quote: ... The protein levels were detected by immunoblot analysis using the primary antibodies: anti-HA antibody (Biolegend, #901503, dilution 1:5000), anti-FLAG antibody (CST ...
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bioRxiv - Genomics 2024Quote: ... A subset of the iPSC lines was evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Cancer Biology 2024Quote: ... tumour cell lines were stained with Zombie Aqua Fixable viability dye (1:1000 in PBS) and for NK ligand (Biolegend) expression in PBS containing 5% FBS and 0.1% sodium azide for 20-30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and 0.75 ug of GAD and Empty tetramers in addition to 1:200 anti-human CD19-BV421 (BioLegend, Cat#363017) and 1:100 anti-human CD3-FITC (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... Single-cell suspensions were generated as described above with an additional incubation with anti-CD11b-BV421 (1:200, Biolegend #101251) and anti-Ly6g-AF647 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... peptide-specific T cells were evaluated by FCM by staining with PE-conjugated MART-1(A27L) tetramer and anti-CD8 FITC (BioLegend) (Supplementary Table 1).
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bioRxiv - Immunology 2024Quote: ... It exhibits monocyte morphology and was originally derived from malignant cells obtained from the pleural effusion from a patient with histiocytic lymphoma.77 LFA-1 (CD11a/CD18) was assessed with a FITC anti-human antibody (clone m24, Biolegend), and MAC-1 (CD11b/CD18 ...
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bioRxiv - Cancer Biology 2024Quote: Single cell suspensions from tumors were washed with HBSS containing 2% FBS and 1 mM EDTA and stained with the following antibody cocktails for detecting lymphoid populations: anti-CD45-PerCP/Cy5.5 (BioLegend 103132), anti-NK1.1-FITC (BioLegend 108706) ...
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bioRxiv - Cancer Biology 2024Quote: 1 × 106 target cells (B16-F10 and Hepa1-6) were suspended in 1ml DPBS containing 5 µM Calcein-AM (BioLegend) and incubated at 37℃ for 30 minutes in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixation and permeabilization was then performed for 1 hour at 4°C using the CytoFast Fix/Perm Set (426803, Biolegend). After fixation/permeabilization ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the MDSC panel consisted of the following primary antibodies diluted in FACS buffer: anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times for 5 minutes each with 1xPBS + 0.03% Triton before incubating for 2 hours in secondary solution containing 2% NDS + DAPI (1:1000; Biolegend 422801) + secondary antibodies (Alexafluor 647 donkey anti-rabbit ...
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bioRxiv - Plant Biology 2024Quote: ... which were then probed with the following primary and secondary antibodies with the indicated dilutions: αHemagglutinin (HA, 1:2000, BioLegend), αGFP (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the Macrophage panel consisted of the following primary antibodies diluted in FACS buffer (2% FBS in DPBS) with anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... and 2.5 mM HEPES into 6-well plates pre-coated for more than 1 h with 5.4 µg of Ultra-LEAFTM purified anti-mouse CD3e antibody (clone 145-2C11, Biolegend, # 100340) and 3 µg of Ultra-LEAFTM purified anti-mouse CD28 antibody (clone 37.51 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then stained with Brilliant Violet (BV) 421TM anti-human CD138 (Syndecan-1) antibody (BioLegend catalog #356516, clone MI15), FITC anti-human HLA-A ...
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bioRxiv - Immunology 2024Quote: ... in the presence of anti-Ly6G AF488 (1A8) and anti-CD68 AF647 (FA-11) or their isotypes (1/100 each, Biolegend), and counterstained using 500ng/mL DAPI dilactate (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... The cell suspension was stained using Brilliant Violet 785™ anti-mouse CD45 antibody (1:100, Biolegend, Clone 30-F11), Brilliant Violet 421™ anti-mouse F4/80 antibody (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stimulated with anti-CD3/CD28 (3.5 µg/ml anti-CD3 mAb, BioLegend, #100339; 1 µg/ml anti-CD28 mAb, BioLegend, #102115) in the presence of 5 μg/mL Brefeldin A (BFA ...
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bioRxiv - Cancer Biology 2024Quote: ... The viability of T-ALL#1 cell suspension was assessed by flow cytometry with Zombie Aqua Viable dye (423102, BioLegend) prior to mouse injection ...
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bioRxiv - Immunology 2024Quote: ... 0.5-1×106 cells were resuspended in 50 μL/well T cell stimulation media (5 μg/mL anti-mouse CD28 (Biolegend), 5 ug/mL BFA) ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 500 xg for 7 minutes and resuspended in 1 mL ice cold Cell Stain Buffer (CSB, BioLegend). Samples were filtered through a sterile pre-wet 70 μm mesh ...
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bioRxiv - Immunology 2024Quote: ... Cellular debris and red blood cells were removed by the Debris Removal Solution (Miltenyi) and 1× RBC Lysis Buffer (Biolegend), respectively ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then incubated 0.1 % BSA in PBS with or without 1/40 dilution of PE/Cyanine7-conjugated anti-human IL6 (Biolegend, #501119) or 1/80 dilution of PE-conjugated anti-human IL8 (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... Then 1×106 cells were washed again with 1x Hanks buffer and stained with 5µl of Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1) Antibody (Biolegend#125608) in100µl BD Pharmingen™ Stain Buffer (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Staining of the red blood cell membrane was performed with the anti-Ter119::Alexa647 antibody (Biolegend, 0.5 mg/ml; 1/1000) for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were incubated with the following primary antibodies diluted in blocking buffer overnight at 4°C – chicken anti-keratin 14 (1:800, Biolegend 906001), rabbit anti-SCD1 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with 1.5 μg/ml Brilliant Violet 605™ anti-CD16 (clone: 3G8) and 1 μg/ml APC anti-CD18 (clone: 1B4/CD18) (Biolegend). Cell data was collected using an Attune flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... ENR media was supplemented with 500 ng/ml rAspn and 1 μg/ml of ultra-LEAF™ CD44 function blocking antibody (Clone IM7; Biolegend) or equal amount of isotype control IgG2b kappa (Clone RTK4533 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were pelleted by centrifugation and then resuspended in 100 μL FACs buffer (PBS with 10 % FCS) with PE-conjugated anti-CD61 (RRID:AB_313084, Biolegend, 104307, 1:200) and Alexa Fluor 647-conjugated anti-SSEA1 (RRID:AB_1210551 ...
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bioRxiv - Immunology 2022Quote: ... 50 μl of supernatants from cultures of T cell clones were collected after 48 hrs of stimulation and added to each well of 96-well round-bottom plates precoated with IFN-γ (clone MD-1) capturing antibodies (BioLegend). After overnight incubation ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with the following primary antibodies overnight at 4 : mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302) + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked using PBS/1% BSA/0.5% Tween-20 and incubated with monoclonal mouse FITC anti BrdU (Biolegend, clone 3D4) at 1:50 dilution for 20 minutes ...
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bioRxiv - Genomics 2020Quote: ... and resuspended in a solution of FACS buffer with a 1:100 dilution of PE anti-human B2-microglobin (Biolegend 316306). Cells were incubated for 30 mins in the dark at 4 °C ...
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bioRxiv - Genomics 2019Quote: ... Isolated nuclei were labeled with an Alexa Fluor 647 anti-GFP antibody (338006, clone FM264G, 1:25, BioLegend, San Diego, CA) for 30 minutes and 2 μg/ml DAPI immediately prior to sorting ...
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bioRxiv - Immunology 2019Quote: ... The cells were incubated with the following antibodies (1:100) for 30 minutes at room temperature: Alexa Fluor 488 anti-mouse CD25 (Biolegend, 102017), PE/Cy7 anti-mouse/human CD44 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... The following antibodies were added (1:100) to each sample and incubated for 30 minutes at room temperature Alexa Fluor 700 anti-mouse CD8a (Biolegend, 100730), PE/Dazzle-594 anti-mouse CD4 (Biolegend ...