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Citations for BioLegend :
7751 - 7800 of 7947 citations for Rat Caprin 1 CAPRIN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... against intracellular antigens of interest were added and incubated for 90 minutes at 4°C (IFNγ: PE-Cy7, clone XMG1.2; IL2: PE, clone JES6-5H4; TNFα: APC, clone MP6-XT22; all Biolegend. all 1:200). Next ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Neuroscience 2021Quote: Nrxn1 neuron cKO-T brain slices (coronal and sagittal) were labeled for Nrxn1 (Fig. 1c-d) using purified HA-647 (mouse, 1:500, Biolegend, Cat# 682404) as described above ...
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bioRxiv - Biophysics 2020Quote: The antibodies used for these experiments were mouse purified anti-HA.11 Epitope Tag monoclonal (clone 16B12) (Biolegend 901502; 1:500 dilution), rabbit affinity-isolated anti-FLAG polyclonal (Sigma-Aldrich F7425 ...
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bioRxiv - Biochemistry 2020Quote: ... Cells at a density of 2×107 cells/mL were incubated for 1 hour in flow cytometry buffer with 5 μL of Pacific Blue™ conjugated anti-CD235a antibody (BioLegend #349108) and 4 μg of unconjugated mouse IgG2a (Merck #M5409 ...
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bioRxiv - Systems Biology 2020Quote: ... up to 1×106 EnSC/100 µL of Magnetic Bead Buffer (0.5% BSA in PBS) were combined with 5 µL/1×106 cells phycoerythrin (PE) conjugated anti-human SUSD2 antibody (BioLegend, London, UK). After 20 min on ice ...
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bioRxiv - Systems Biology 2021Quote: Cells were stained overnight at 4°C in blocking solution with the following antibodies: AlexaFluor® 488 anti-Vimentin (1:1000, Biolegend, #677809), AlexaFluor® E-cadherin (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... catalog number L100-L115-01) in PBS with 1% BSA and incubated the beads with 3.5 µg/ml biotinylated anti-CD81 antibody (BioLegend, clone Eat-2) at the room temperature for 40 minutes ...
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bioRxiv - Cell Biology 2022Quote: Whole lung single cell suspensions were prepared as above and then blocked in SB containing 1:50 TruStain FcX™ (anti-mouse CD16/32) Antibody (BioLegend, #101319) for 10 min at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 5×105 cells were stained for 30 min using the Zombie Aqua fixable viability dye (BioLegend Cat# 423101, 1:500 dilution), then fixed for 1 h in Foxp3/Transcription Factor Fixation/Permeabilisation Solution (ThermoFisher Cat# 00552300) ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated overnight at 4°C with purified monoclonal mouse anti-human ASC antibodies (TMS-1) (clone HASC-71) (Biolegend, California, USA) at a final concentration of 10 µg/ml diluted in 10% NGS + 0.5% Triton X-100 in PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... samples P008-016 and P048-119 (Suppl. Fig. 4D) were labelled with TotalSeq B Hashtag antibodies 1-4 (Biolegend, clone LNH-94) during the staining procedure described above and pooled in groups of four.
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bioRxiv - Immunology 2020Quote: ... OVA and HEL were biotinylated in house to ∼1 biotin per antigen molecule and tetramers constructed as described [14] using streptavidin-PE (Biolegend, CA, USA), streptavidin-APC (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... and anti-mouse CD44(CD44-APC - cat. 137607, clone 29A1.4, LOT B152186, dilution 1:200) were purchased from BioLegend (BioLegend, London, UK). Antibody dilutions in cell staining buffer containing 1 % TruStain fcX™ (anti-mouse CD16/32 ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in 10% FCS in PBS (100μl/well) containing TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend; 1:200) and incubated on ice for 20mins ...
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bioRxiv - Immunology 2019Quote: ... TCRβ (H57-597, BV605), NK-1.1 (PK136, BV650), and PD-1 (RPM1-30, PE/Dazzle 594) were purchased from BioLegend (San Diego, CA). Antibodies against CD4 (GK1.5 ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed with 1 ml of PBS and finally resuspended in 100 µl of FACS buffer (1% BSA in PBS with Ca2+/Mg2+) with anti-HMGB1 antibody (10µg/ml, BioLegend, catalogue number-651402). The cells were incubated at 4 °C for 30 minutes on ice and were washed with 1 ml PBS (containing Ca2+/Mg2+) ...
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bioRxiv - Plant Biology 2021Quote: ... Biotin labeled DNA was detected with 1:5000 solution of an anti-biotin HPR-conjugated antibody (BioLegend, San Diego, California, United States) in TBST with 5% BSA ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: In order to separate cells of different origin we further stained the cell suspension with antibodies against CD45 (Biolegend #103107; 1:200), FAP (Abcam #ab28244 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspensions were incubated for 20 minutes on ice with anti-CD16/CD32 to block Fc receptors (1:50; BioLegend Cat #: 553141) and with a viability dye ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... Human pluripotent stem cell-derived cardiomyocytes used for IF-FISH were sorted by gating for SIRPA+ (PE-Cy7 anti-human CD172a/b, Biolegend, 1:200) and CD90- (APC anti-human CD90 (Thy1 ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 μL of CytoC stain buffer (2% Tween20, 10% BSA (w/v) in PBS) + 1:400 Alexa Fluor 647 anti-cytochrome c antibody (Biolegend, cat#612310) + 1:100 DAPI (1 mg/mL ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed 3x with PBS and incubated with a cocktail of primary antibodies to intracellular compartment markers - clathrin (1:500, Biolegend MMS-423P), caveolin (1:200 ...
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bioRxiv - Immunology 2021Quote: ... a set of plated moDCS were treated with an anti-HLA-A/B/C antibody (40 μg mL−1, Clone W6/32, Biolegend Cat 311428) or anti-class II (10 μg mL−1 ...
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bioRxiv - Immunology 2021Quote: ... infarct macrophages as CD45+/CD11b+/Ly6G−/F4/80+ cells and infarct neutrophils as CD45+/CD11b+/F4/80−/Ly6G+ cells.7–9 The following antibodies were used: anti-CD45 (clone 104, 1:100, BioLegend/BD Biosciences); anti-F4/80 (clone BM8 ...
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bioRxiv - Cell Biology 2020Quote: ... a 6-colour flow cytometry panel was performed on a Bio-Rad ZE5 Cell Analyzer using antibodies for CD56 (Clone HCD56, Brilliant Violet 605, BioLegend, 1:200), CD3 (Clone SK7 ...
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bioRxiv - Neuroscience 2021Quote: ... that recognizes residues 17–24 of Aβ and the same sequence in APP full length (BioLegend San Diego, California, USA; 1:1000); mouse C-term APP Y188 (Abcam ...
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bioRxiv - Bioengineering 2022Quote: ... The samples were treated with 3% bovine albumin in PBS containing 0.3% Triton X-100 and then stained with CD31 antibody conjugated with Alexa 647 (1:100 dilution, Biolegend, San Diego, CA) and EMCN antibody conjugated with PE (1:50 dilution ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:50 diluted anti-human CD45-AF700 (Biolegend, clone HI30), 1:100 diluted anti-human CD3-FITC (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... each sample was pelleted at 1,150 x g in a centrifuge for 5 min at room temperature and then stained with 50 µL of an antibody cocktail containing 1:100 diluted anti-human CD3-FITC (BioLegend, clone UCHT1), 1:100-diluted anti-human CD4-PE (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... lymph node FNA samples collected 2 or 8 weeks post-boost were stained for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... Pelleted nuclei were suspended in Nuclear Stain Buffer (Sucrose Buffer containing 1:4,000 Alexa Fluor® 647 anti-Nuclear Pore Complex Proteins Antibody (BioLegend, Cat# 682204) plus 4µM ethidium homodimer (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... GAPDH was used as an internal control (1:1000, Direct-Blot™ HRP anti-GAPDH antibody, 607903; Biolegend, San Diego, CA, USA). Signals were visualized using Luminata Forte (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... the pellets were resuspended and incubated for 15 min on ice in 0.5 ml MEM containing CD24-APC antibody (1:500, 101814, Biolegend, San Diego, CA). After centrifugation for 5 min at 200 × g at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were incubated with the following antibodies for 30 min at 4°C: CD45.1-PerCP-Cy5.5 (BioLegend, Catalog No. 110728, 1/500), CD45.2-BV650 (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... The APC depleted fraction was collected and used for staining with the following antibodies: Ter119-FITC (BioLegend, Catalog No. 116205, 1/800), Cd45R-FITC (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...
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bioRxiv - Immunology 2024Quote: ... The CD11c-APC-depleted fraction was further incubated with anti-biotin beads (Milteniy Biotec, cat# 130-090-485) and streptavidin-PE/Cy7 (BioLegend, 1:500), followed by another MACS enrichment using MS columns ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated 72h at 4°C on a rotating wheel with the secondary antibody (1:500, Alexa-Fluor-555, 405324 P4U/BioLegend UK Ltd) diluted in 100% CAS-Block ...
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bioRxiv - Immunology 2023Quote: ... IL-1b, IL-6, IL-10, IL-17A, IL-12p70, GM-CSF, IL-23, IFN-b, MCP-1, IL-27; BioLegend Cat# 740446), Mouse TH Panel v4 (TNF-α ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibodies used and the dilutions were as follows: for neurons: anti-Tubulin-βIII Rabbit mAb (1:200, BioLegend, 802001, clone TUJ1); anti-Synapsin-1 Mouse mAb (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells that have been incubating with conditioned media were carefully transferred to the CD3-coated plates and further stimulated with 1 µg/mL anti-CD28 (BioLegend, clone: CD28.2) for another 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... the CNP labeled cells were spin washed and incubated with 10μgꞏml−1 of AF488 conjugated anti-CD3 antibodies (Biolegend, Cat # 300415, clone UCHT1) in 2% BSA/PBS on ice for 30min and then spin washed ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with the following antibodies in FACS staining buffer (PBS with 2% Fetal Bovine Serum, 1% Penicillin-Streptomycin): anti-human CD31 (BioLegend, WM59, FITC), anti-human CD45 (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... we triple-washed samples with 0.1% PBS-Tw and labeled them with streptavidin-BV510 (Biolegend, 405234; 1% BSA in 0.1% PBS-Tw). After a 30-min incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor single-cell suspensions were stained with Alexa Fluor 700 anti-CD326 (Ep-CAM) antibody (Biolegend, 118239, clone: G8.8, 1:100 dilution), PE/Cyanine 7 anti-mouse CD31antibody (Biolegend ...