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Citations for BioLegend :
7651 - 7700 of 7730 citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... All cytokine antibodies were used at 1:100 dilution in 1x BD Perm/Wash buffer (anti-IFN-γ (APC/Cyanine7 or PE, Biolegend, #505849, #505808) and anti-TNF-ɑ (BV711 Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... Extracellular deposits of Aβ peptide were immunostained with the mouse anti-β-amyloid 17-24 antibody (1:5000, clone 4G8, BioLegend, Koblenz, Germany). Alpha-synuclein pathology was detected with the anti-syn-1 antibody (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... was added to measure and gate on the % live HL-60 cells and anti-mouse/human CD11b PE (Biolegend, Catalog #101207, 1:50) was added to measure and gate on differentiated HL-60 cells ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... Cells were incubated with a TotalSeq-C antibody cocktail as well as hashtag antibodies to allow for post sequencing differentiation of distinct expanded ILC subsets(BioLegend, Supplemental Table 1) for 30min ...
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bioRxiv - Bioengineering 2023Quote: ... coated glass bottom chamber and then labeled with 1ugꞏml−1 of Alexa Flour (AF) 488 conjugated anti-human CD45 antibodies (Biolegend, Cat# 304017, clone HI30) in 2% BSA in PBS for 20 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... singlesuspension cells were stained at the cell surface in different panels with PerCP5.5-conjugated anti-CD45 (1:100, BioLegend Cat# 103132 (also 103131), RRID:AB_893340) ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used to validate siRNA knock-down in fibroblasts: anti-α2-spectrin (mouse IgG2b clone D8B7, Biolegend #803201, 1:1000), anti-β2-spectrin (BD Biosciences #612563 ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with the following primary antibodies and dilutions: anti-Nestin (1:150, Biolegend, #656802, clone 10C2), anti-EGFR (1:300 ...
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bioRxiv - Cancer Biology 2023Quote: ... NKG2D and DNAM-1 surface receptors on NK cells were blocked utilizing 25 μg/ml of purified anti-mouse NKG2D (Biolegend, clone CX5, 130202), purified anti-mouse DNAM-1 (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25 ...
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bioRxiv - Immunology 2024Quote: ... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
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bioRxiv - Cell Biology 2024Quote: ... non-specific Fc receptor binding was blocked using 1:100 dilution of TruStain FcX anti-mouse CD16/32 antibody (BioLegend, RRID:AB_1574975, Cat#101320). 5 min after Fc blocker treatment ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the NK-92 staining panel: anti-CD107a (LAMP-1) FITC (BioLegend catalog #328606, clone H4A3), anti-CD56 (NCAM ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were pretreated with 100 U/mL IFN-γ for 24h and stained with 1:1000 H2kb-SIINFEKL Ab (BioLegend,Clone:25-D1.16). GFP-expressing cell lines were used for GFP compensation ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended by mild vortexing and 50 ul of 2.5 µg/ml final (meaning 1/200 dilution of the stock) Fc-block (anti-mouse anti-CD16/32 antibodies, TruStain FcX from Biolegend, San Diego, CA) were added with the following addition of 1/200 dilution of antibodies for surface marker staining and Aqua Live/Dead dye (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... collected into FACS tubes (Falcon, cat no. 352052) and incubated with the following antibodies: AF700-labeled CD54 (1:100, BioLegend, cat no. 353126) and PE/Cy7-labeled CD62E (1:100 ...
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bioRxiv - Immunology 2020Quote: ... ASC was stained using a primary antibody anti-ASC (polyclonal anti-PYD ASC, Santa Cruz or monoclonal anti-CARD ASC, Biolegend; 1:1000 dilution) or anti-Luciferase (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized with ice-cold methanol at −20°C for at least 20 min and incubated with anti-Pol II antibody (Biolegend, 8WG16 at 1:200), anti-PABPC antibody (Abcam ab21060 at 1:200 for mouse cells and Santa Cruz sc32318 at 1:25 for human cells ...
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bioRxiv - Cell Biology 2021Quote: ... and the cell pellet was resuspended in 1 volume (V) CnT-PR-MSC-XF-HC before adding 3V of 1x Red Blood Cell Lysis Buffer (Biolegend, San Diego, United States). After 10 minutes of incubation at room temperature another 10V of CnT-PR-MSC-XF-HC were added and the cell suspension was centrifuged ...
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bioRxiv - Microbiology 2021Quote: Concentrations of cytokines and chemokines present in the serum from SARS-CoV-2 infected AGMs were quantified using a multiplex bead-based assay (1:4 dilution)-the LEGENDPlex Non-Human Primate Cytokine/Chemokines 13-plex (BioLegend, San Diego, CA USA). Analytes detected by this panel are the following ...
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bioRxiv - Cancer Biology 2022Quote: ... Human tissues were incubated for 25 min at 4 °C with the following primary antibodies: CD24-PE (BioLegend, clone ML5, Cat, #: 311105, 1:100), CD44-FITC (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Staining for human immunoglobulins was performed in 100 μL with 1:50 (v/v) of the detection antibodies: anti-human IgM Brilliant Violet 650 (clone: MHM-88, Biolegend® Cat. No. 314526), anti-human IgG PE/ Dazzle™ 594(clone ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in PBS containing Zombie Violet dye (Biolegend; 1:200 in PBS; 100μl per well) and incubated at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... at a dilution of 1:200 for 30 min at room temperature followed by incubation with horseradish peroxidase streptavidin at a dilution of 1:125 (405210, BioLegend, San Diego, CA, USA) for 30 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...
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bioRxiv - Immunology 2020Quote: ... LNs were harvested and digested as below (preparation of single cell suspensions) and stained with CD45 brilliant violet 510 (Biolegend clone 30F11, 1:300), PDPN APC (Biolegend clone 8.1.1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue was incubated in primary antibody overnight at 4°C in a moist chamber diluted in blocking buffer with the following dilutions: Anti-Tubulin β3 (BioLegend, clone TUJ1, 1:500); Anti-OMP (Santa Cruz ...
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bioRxiv - Cancer Biology 2022Quote: ... the antibody or IgG1 excess was rinsed and pellets were resuspended in APC-conjugated Annexin V at 1:20 in Annexin V buffer solution plus Zombie Violet dye at 1:400 (all antibodies and probes are from Biolegend, San Diego, CA, USA). Samples were transferred into FACS tubes and incubated for 20 minutes at room temperature protected from light prior to their analysis by FACS Canto II (BD ...
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bioRxiv - Immunology 2022Quote: Antibody staining was done in the dark for 30 minutes at 4°C in 30 μL of FACS buffer with antibodies and anti-CD16/32 (TruStain FcX™ PLUS; BioLegend, #156604; 1:100). Acquisition was done on a BD FACSCanto™ II or BD LSRFortessa (both BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: BALF and post-digested lungs were centrifuged at 1500 rpm before resuspension in 100 μL of a 1:100 dilution of Zombie Yellow™ dye (Biolegend, San Diego, USA). After staining ...
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bioRxiv - Bioengineering 2024Quote: ... The sections were subsequently stained with a rat anti-human YAP antibody conjugated with PE (dilution: 1:500; clone W19260A; Biolegend, San Diego, CA, USA) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... peripheral blood and spleens were harvested from each mouse and the infiltrated primary cells were identified using flow cytometry by staining for human CD45 antibody (BioLegend; BLG-368522; 1:200).
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using the following antibodies: Brilliant Violet 711™ anti-human CD4 (344648, BioLegend; 1 μL/well), APC anti-human CD3 (300412 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were combined in different panels comprising of an appropriate selection from the following: a 1:400 dilution of Alexa Fluor™ 750 anti-murine CD8 (BioLegend, Cat. No. 100766), a 1:400 dilution of Brilliant Violet™ 605 anti-murine CD4 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... BV-421), CD62L (clone: MEL-14, PE), PD-1 (clone: 29F.1A12, PE-Cy7), CD107a (clone: 1D4B, APC) from BioLegend (San Diego, CA, USA), and NKG2D (clone ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Immunology 2022Quote: ... 4 °C) cells were stained with the following conjugated antibodies for 20 min at RT in the dark: CD3-APC (Biolegend, clone REA613, 1:200), CD11b-APC-Cy7 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... were blocked with 5% BSA and 5% goat serum in PBST (PBS/0.25% Triton X-100) for 30 min and then incubated with monoclonal 6E10 antibody (BioLegend, cat# 803001, 1:1000 dilution) overnight at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... the slices were transferred into 30% sucrose in water for >24 hours and then sub-sectioned on a sliding microtome to 15-30 μm for immunostaining using the following antibodies: mouse anti-HA (Biolegend catalog #901513, 1:1000) and rabbit anti-NeuN (Millipore catalog #ABN78 ...
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bioRxiv - Immunology 2023Quote: ... pancreatic islets were harvested from four 12-week-old female NOD mice and labelled individually with anti-TCRβ (BioLegend, allophycocyanin, H57-597, 1:300), anti-CD4 (BioLegend ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.2 g/L NaN3] for 15 min after which the grids were incubated on a 20 μl drop of primary antibodies—1:10 dilution of 6E10 (Biolegend, San Diego, CA, USA) and anti-αSyn MJFR1 (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Isolated aortic single cells were stained with Fc receptor blocker (TruStain FcX anti-mouse CD16/32; 101319; BioLegend, San Diego, CA, USA, 1:100) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained with unconjugated rat anti-mouse lineage-specific antibodies (Ter-119, Mac1, Gr-1, B220, CD5, CD3, CD4, CD8, and CD127, Biolegend, San Diego, CA, USA), followed by incubating with Dynabeads anti-rat IgG (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized with 0.1% Triton X-100 in PBS (5 min at RT) and immunolabeled with anti-HA (Biolegend, 901501; 1:500 in PBS) for 1 hr at RT ...
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bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Microbiology 2023Quote: ... washed once with PBS and stained for 30 min at RT in the dark with anti-CD4 antibody (PerCP-Cy5.5, Biolegend #317428, 1:50 in PBS) and eBioscience Fixable viability dye 780 (ThermoFisher Scientific #65-0865-14 ...
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bioRxiv - Cancer Biology 2023Quote: ... or the following antibody panel where the 488 and 555 channel markers were swapped: Alexa Fluor® 488 anti-Nestin (1:150, Biolegend, #656812, clone 10C2), Alexa Fluor® 555 anti-S100 beta (1:1000 ...