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Citations for BioLegend :
7301 - 7350 of 7730 citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4°C with the following antibodies: CD138-FITC (1:20; MI15, BioLegend), CD45-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Immunology 2022Quote: T cells were adjusted at a concentration of 1 × 106 cells/100 μl CTL medium including Golgi-Stop (Biolegend, 420701) and Golgi-Plug (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45lowSiglec-H+ microglia were labeled with rat anti-ICAM1 BV711 (1:100, catalog no. 116143; BioLegend), rat anti- Ly6A/E FITC (1:100 ...
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bioRxiv - Developmental Biology 2023Quote: ... When staining for SLAM markers the same lineage cocktail was used (FITC) with the addition of the following antibodies: anti-CD48 (FITC, clone HM48-1, BioLegend), anti-CD41 (FITC ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
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bioRxiv - Microbiology 2023Quote: ... for 1 hour at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
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bioRxiv - Microbiology 2023Quote: ... Adjacent sections were deparaffinized in citrate and stained with monoclonal mouse anti-F4/80 antibody at a 1:400 dilution (BioLegend), or polyclonal goat anti-MPO antibody at a 1:500 dilution (R&D Systems) ...
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bioRxiv - Immunology 2023Quote: ... washed FACS buffer and stained with a 1:100 dilution of anti-mF4/80 or anti-m/hCD11b antibody (Biolegend) FACS buffer for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Pacific Blue (or v450), and APC-Cy7 (or Alexa Fluor-e780)-conjugated antibodies (1:100, CD4, CD8) purchased from Biolegend. After cell surface marker staining ...
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bioRxiv - Immunology 2023Quote: ... the slides were incubated for 1 hour in the dark at room temperature with an Alexa 488-conjugated goat anti-mouse IgG antibody (BioLegend). Following three washing steps ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... stained with a mixture of fluorochrome-conjugated antibodies (1:200 FITC anti-mouse/human CD11b M1/70, BioLegend, California, USA), (1:200 APC/Cyanine7 anti-mouse F4/80 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then permeabilized using BD Fix/Perm for 20 min at RT and stained intracellularly with anti-IFN-γ (1:100 dilution; clone XMG1.2, Biolegend), anti-TNF-α (1:100 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... followed by staining with Brilliant Violet (BV) 785 conjugated anti-human CD69 (10 μgꞏml−1) (Biolegend, Cat # 310932, clone FN50) for 30 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cell suspensions were counted and the same number of cells (106 cells/100 µL) were stained with 1:100 dilution of anti-CD45 (157610, BioLegend) antibody and DAPI ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 106 cells were incubated with PE anti-CD31 ([WM59] 303105), PE anti-CD45 ([2D1] 368510) and PE anti-CD326 (EpCAM, [9C4] 324206) antibodies (1/100, BioLegend) and human TruStain FcXTM (1/200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then diluted with 50 μL PBS + 2% FBS and FITC anti-CD45 (BioLegend, 304005; 1:1,000 final dilution) and incubated for 20 min on ice in the dark ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with Zombie Near IR at a 1:1000 dilution in PBS following the manufacturer’s instructions (Biolegend, #423117). Cells were then fixed ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were centrifuged at 500× g at 4 °C and resuspended in 1/3 strength artificial seawater containing 2 μg/mL calcein AM (Enzo) and 100 nM sytox blue (BioLegend) to monitor viability ...
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bioRxiv - Genomics 2022Quote: ... all cells for Perturb-seq gRNA enrichment screens were stained with Zombie NIR fixable viability dye 1:1000 dilution in PBS (Biolegend). Cells were analyzed by flow cytometry (Attune Nxt ...
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bioRxiv - Genomics 2023Quote: ... and all the samples were treated with sybr green (1x) and mouse anti-human IgG conjugated with Alexflour 647 (1:100 dilution, from BioLegend) for 30 mins at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and goat anti-rabbit Alexa Fluor Plus 647-coupled secondary antibody (1:500) in Intracellular Staining Permeabilization Wash Buffer (Biolegend). The fraction of cells positive for cleaved caspase-3 was measured with a BD LSRFortessa SORP flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were then pelleted and resuspended in 100 µL of Zombie Green viability dye (1:500 in PBS; BioLegend, 423111). After 15 minutes on ice ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500, 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Satellite cells were FACS-purified using either Pax7-nGFP reporter or the following combinations of cell surface markers: APC anti-mouse Ly-6A/E (Sca-1) Antibody (108111, BioLegend), mouse Integrin alpha 7 Alexa Fluor 750-conjugated Antibody (FAB3518S ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated single-cell suspension and peripheral leukocytes were fluorescently stained with anti-mouse CD11b-FITC (1:200, BioLegend, 101205) and Ly6G-PE (1:80 ...
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bioRxiv - Cancer Biology 2023Quote: ... Hepatocytes were resuspended in 2 mL FACS buffer (2 mM EDTA, 0.5% BSA in PBS) and stained with Zombie Violet (1:500) (Biolegend #423113), TruStain FcX™ (anti-mouse CD16/32 ...
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bioRxiv - Cancer Biology 2023Quote: ... 90% of the thawed cells (>200 x 106) were stained with biotinylated antibodies against CD45 (1:50, clone HI30; Biolegend) and CD235a (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320 ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with a premixed cocktail of the indicated tetramers at a final concentration of 10 nM for 1 hour at RT in the presence of anti-mouse CD16/32 antibody (clone 93, BioLegend). SLO preparations were magnetically enriched for tetramer-specific cells as previously described64 while lung samples were magnetically enriched for CD90.2 expression (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... Cell staining followed a protocol developed in-house consisting of two separate panels of antibodies [Panel 1: CD14 (FITC) (#367116 BioLegend), CX3CR1 (PE ...
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bioRxiv - Cancer Biology 2023Quote: ... then incubated overnight at 4°C with KI67 primary antibody (cell signaling technology) diluted 1:400 or CD8a primary antibody (Biolegend) diluted 1:200 in Antibody diluent signal stain (Dako) ...
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bioRxiv - Developmental Biology 2023Quote: ... An aliquot of the resulting suspensions in PBS were immediately stained for viability with Zombie Aqua diluted 1:1000 (BioLegend) for 20 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer preincubated with streptavidin (30 min at molar ratio of 1:4 at 25°C) conjugated to phycoerythrin (405203, BioLegend) and/or BV421 (405226 ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated for 30 minutes on ice with the following antibody: anti-CD44 APC/Fire 750 (1:100, Biolegend, 103061), anti-CD24 PE (1:20 ...
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bioRxiv - Molecular Biology 2023Quote: Cells were incubated for 30 minutes at room temperature in 0.5% BSA PBS with 1:50 CD81-FITC antibody (Biolegend, 349504) or mouse IgG1 FITC isotype control antibody (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA in HBSS) at 1 x 107 cells/mL and incubated with Fc block (anti-mouse CD16/32; clone 93. BioLegend) for 10 minutes before incubation with specific antibodies listed in Table S1 ...
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bioRxiv - Immunology 2023Quote: Peyer’s patch leukocytes from 10 WT B6 mice de novo colonized for 21 days with SFB were separately stained with oligo-hashtagged antibodies (TotalSeqC 1 – 10, Biolegend), prior to pooling ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies and dilutions used for western blot were: Mouse Anti-Aβ (6E10 clone, 1:1,000, Biolegend, Cat# 803001, RRID:AB_2564653), goat anti-Mouse DKK-3 (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were then maintained for 10 min at 37°C in the presence of AlexaFluor-647-conjugated AnxA5 (1/50, Biolegend) in resting solution (Locke’s solution:140 mM NaCl ...
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bioRxiv - Immunology 2023Quote: ... B cells were gated for using 1:20 dilution commercial mouse anti-human CD19-Alexa488 (BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL ...
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bioRxiv - Immunology 2023Quote: ... the following antibodies were used at a 1:200 ratio: epithelial cells (324216, Biotin anti-human Epcam, clone:9C4, BioLegend), endothelial cells (13-0319-82 ...
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bioRxiv - Genomics 2023Quote: ... Many of the iPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Cancer Biology 2024Quote: 1 × 106 target cells (B16-F10 and Hepa1-6) were suspended in 1ml DPBS containing 5 µM Calcein-AM (BioLegend) and incubated at 37℃ for 30 minutes in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixation and permeabilization was then performed for 1 hour at 4°C using the CytoFast Fix/Perm Set (426803, Biolegend). After fixation/permeabilization ...