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Citations for BioLegend :
7601 - 7650 of 7730 citations for Insulin ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The cell pellets were washed once with 15 mL PBS and resuspended in 1 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 2 min at room temperature to lyse RBCs ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with FACs buffer (1X PBS, 1% FBS) and fixed at room temperature for 20 minutes with Intracellular Staining Fixation Buffer (BioLegend, San Diego, CA). Cells were washed with 1X Cyto-Fast™ Perm Wash solution (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... The stain solution contained a 1:200 dilution of an antibody against H-2Kb bound to SIINFEKL conjugated to PE (#141603, Biolegend, San Diego, CA) to detect SIINFEKL peptide being presented at the cell surface ...
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bioRxiv - Cancer Biology 2019Quote: ... or U2S3-hFc-mutIL15 for two hours before fixing and staining for intracellular phospo-STAT3 (Biolegend PE anti-STAT3 Tyr705 clone 13A3-1) and –STAT5 (BD Alexa Fluor 647 anti-STAT5 pY694 clone 47) ...
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bioRxiv - Microbiology 2021Quote: ... 20 μl cells were removed for flow analysis and remaining cells were incubated for 30 min on ice with 1 μg each of TotalSeq™-A0441 anti-mouse CD71 Antibody (Biolegend, Cat# 113824) and TotalSeq™-A0073 anti-mouse/human CD44 Antibody (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... 50-μm brain sections were blocked with normal goat serum for 2 h and incubated overnight using biotin-conjugated anti-HA (Biolegend 901505, 1:500) and anti-parvalbumin (Synaptic Systems 195 004 ...
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bioRxiv - Biochemistry 2020Quote: ... Traditional antibodies based labeling procedure was done by using c-Myc Antibody (9E10, Cat # 626801, BioLegend, USA; incubation 1 h at 4°C) and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143 ...
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bioRxiv - Neuroscience 2020Quote: ... Axons were identified using a staining against the phosphorylated neurofilaments (SMI31) stained with the PAB mouse-anti-SMI31 (Biolegend, cat. 801601, 1:250) and the SAB GAM-STAR580 (Abberior ...
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bioRxiv - Cell Biology 2021Quote: ... Immunostaining was performed with biotinylated monoclonal mouse antitubulin (clone 236-10501, Thermo, 1 μg/ml) then Brilliant Violet 421-conjugated streptavidin (Biolegend, 0.1 μg/ml), Alexa Fluor 488-conjugated mouse anti-perforin (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... 50μM coronal sections containing dorsal hippocampus were blocked in normal goat serum for 2 h and incubated overnight using a biotin conjugated anti-HA (Biolegend 901505, 1:500), anti-pS6 244-247 (ThermoFisher 44-923G ...
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bioRxiv - Neuroscience 2020Quote: ... Aβ peptides were extracted from 250 µL human plasma using immunoprecipitation with anti-β-Amyloid 17-24 (4G8) and anti-β-Amyloid 1-16 antibodies (6E10, both Biolegend®) coupled to Dynabeads™ M-280 Sheep Anti-Mouse IgG magnetic beads (Thermofisher ...
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bioRxiv - Neuroscience 2022Quote: ... Splenocytes were then filtered through a 40μM filter and stained with an antibody cocktail [Pacific Blue anti-CD45.1 (Biolegend no. 110722; 1:100 dilution), APC anti-CD45.2 (Biolegend no ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Immunology 2022Quote: ... extracellular staining was done in ice-cold buffer (phenol red–free DMEM, 2% FBS) for 30 min using antibodies against CD45 (30-F11; BioLegend, dilution 1:200), CD4 (RM4-5 ...
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bioRxiv - Microbiology 2022Quote: ... single cell suspensions from mouse spleens were stained with fluorescein isothiocyanate (FITC)-conjugated anti-Ly-6A/E (Sca-1) mAb (Biolegend; San Diego, CA). To analyze erythrocyte maturation ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed in PBS/1% BSA buffer and incubated for 30 minutes on ice with the following antibodies from Biolegend (San Diego, USA): FITC-anti-human CD38 (#303503) ...
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bioRxiv - Neuroscience 2020Quote: ... and were stained at 20 μl of FACS buffer per 1×106 cells containing the first set of following antibodies (all from Biolegend, unless specified otherwise): biotinylated hematopoietic lineage antibodies against NK1.1 (clone PK136) ...
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bioRxiv - Immunology 2020Quote: A total of 1×106 splenocytes or liver lymphocytes were surface stained with Brilliant Violet (BV) 421-conjugated anti-CD107a (clone 1D4B, BioLegend, San Diego, CA) during the 24 h stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... and the nerve sections were incubated for 24 hours at room temperature with gentle rocking: mouse anti-TUJ1 for microtubules in the axons (1:1000, Biolegend Catalog No. 801202), rat anti-PECAM-1 for endothelial cells (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Developmental Biology 2022Quote: ... MGs for whole mount samples and cryosections were stained with a rabbit anti-keratin 14 (K14) polyclonal antibody (1:1000, 905301, BioLegend, San Diego, CA). MG whole mounts and cryosections were imaged with a Zeiss LSM880 confocal microscope ...
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bioRxiv - Immunology 2019Quote: ... cells were washed with 200 μl FACS Buffer and blocked in 100 μl Fc block (cat. no. 101319, TruStain FcX, clone 93, BioLegend, diluted 1:100) in FACS Buffer for 10 min on ice and washed ...
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bioRxiv - Systems Biology 2019Quote: ... We washed the samples again with 0.1% BSA-PBS and then incubated for 10 minutes at 4°C with 1:250 anti-NGFR antibody conjugated directly to PE-Cy7 (Biolegend, 400126, clone ME20.4) in 0.5% BSA-PBS with 2mM EDTA ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly harvested PDX or EHS tumors were processed to single cells47 and stained with PE-conjugated anti-human CD298 PE (BioLegend; 1:50 dilution) and FITC-conjugated anti-mouse CD45 FITC (BioLegend ...
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bioRxiv - Neuroscience 2019Quote: ... The triangularis sterni muscle was dissected and incubated overnight (4°C) in anti-βIII-tubulin antibody conjugated to Alexa488 (BioLegend 801203; 1:200) and Alexa594-conjugated α-bungarotoxin (Invitrogen B13423 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... Cells with successful PD-L1 deletion were isolated by two rounds of FACS sorting after O/N stimulation with 100 ng/ml IFNγ and staining with PD-L1-PE/Cy7 (clone 10F.9G2, Biolegend 124314, 1:200). Cells retaining PD-L1 expression were isolated simultaneously and served as controls.
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with Fc-blocking anti-CD16/32 anti-bodies followed by cell hashing antibody (1 μg/ml, BioLegend Cell Hashing A) (Stoeckius et al. ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACS buffer followed by PBS wash and stained with 100 μl of 1:1000 dilution of fixable Zombie Aqua (BioLegend, San Diego, CA) dye for 20 minutes at 4°C in dark ...
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bioRxiv - Immunology 2020Quote: ... and then incubated in the dark for 30 min at 4°C: Myeloid-lymphocyte common lineage panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend, San Diego, CA), CD45-Pacific Blue (clone ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 Satb1 cKO) of varying age 1-7 months by the LEGENDplex (13-plex) Mouse Th Cytokine Panel V02 (Biolegend, 740741; Lot B289245) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed once in PBS and then fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) before staining with PU.1-PE (BioLegend 7C2C34 cat #681307) or rat IgG2a-PE isotype (BioLegend RTK2758 ...
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bioRxiv - Cancer Biology 2022Quote: ... stained for 20 minutes on ice with the following antibodies at the following concentrations: anti-mouse CD49b 1:500 (#103511 Biolegend, San Diego, CA), anti-human CD49b 1:750 (#359310 Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... a sequential gating strategy was used to analyze cancer cells by staining with the following FACS antibodies (Figure S3F): CD45-Pacific Blue (BioLegend 103126, 1:100), CD31-Pacific Blue (BioLegend 102422 ...
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bioRxiv - Cell Biology 2022Quote: ... obtained single cell suspensions were stained with the fixable viability Zombie Aqua™ dye according with the manufacturer instructions (#423101; BioLegend, 1:500).
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bioRxiv - Neuroscience 2022Quote: ... human Aβ was immunodepleted from TgCM with the mouse antibody 6E10 (Purified anti-β-Amyloid, 1-16 antibody BioLegend Cat# 803004, RRID: AB_2715854) and Protein G Sepharose beads (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed with 15 mL of wash buffer (RPMI with 1% FBS) and the CD4+ T cells were enriched using MojoSort™ Mouse CD4 cell isolation (Biolegend, Cat#480006). The cells were stained and sorted as explained before in scRNAseq section as well ...
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bioRxiv - Immunology 2022Quote: ... T cells were added to anti-CD3 coated wells at 106 cells/mL in the presence of 1 μg/mL anti-CD28 (Biolegend, LEAF purified, 37.51), 100 U/ml IL-2 (gift from K ...
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bioRxiv - Microbiology 2024Quote: ... The panel of cytokines presented in Fig 5 was measured using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) (BioLegend, Cat# 740809) kit ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
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bioRxiv - Immunology 2024Quote: ... and incubated at 37°C for 30 min with either a 1/500 dilution of PE-labeled anti-6xHis tag mouse MAb (Biolegend, San Diego, CA) to detect VHHs or Alexa Fluor 647-labeled goat anti-human IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... quantification of PG release from cartilage explants was performed using the dimethylmethylene blue assay (36, 37) and exposure to sialidase (1.9 U/mL) and interleukin-1 beta (Cat #. 300-01A; BioLegend, San Diego, CA, USA) (10 ng/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mg of protein G agarose beads were crosslinked to 5 μL of mouse anti-HA antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) using an effective concentration of 6.5 mg mL-1 DMP ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were suspended at 3×106 cell/ml and incubated for 30 min at 4C with 1:100 dilutions of the following antibodies: [APC dump channel: Ter-119 (Biolegend cat no. 116212), CD45 (Biolegend cat no ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Neuroscience 2023Quote: ... The following monoclonal antibodies were used for flow cytometry analyses at a dilution of 1/700: anti-CD45 (BioLegend, clone 30-F11, 103147), anti-CD11b (BioLegend ...