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Citations for BioLegend :
7551 - 7600 of 7605 citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... LNs were harvested and digested as below (preparation of single cell suspensions) and stained with CD45 brilliant violet 510 (Biolegend clone 30F11, 1:300), PDPN APC (Biolegend clone 8.1.1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue was incubated in primary antibody overnight at 4°C in a moist chamber diluted in blocking buffer with the following dilutions: Anti-Tubulin β3 (BioLegend, clone TUJ1, 1:500); Anti-OMP (Santa Cruz ...
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bioRxiv - Cancer Biology 2022Quote: ... the antibody or IgG1 excess was rinsed and pellets were resuspended in APC-conjugated Annexin V at 1:20 in Annexin V buffer solution plus Zombie Violet dye at 1:400 (all antibodies and probes are from Biolegend, San Diego, CA, USA). Samples were transferred into FACS tubes and incubated for 20 minutes at room temperature protected from light prior to their analysis by FACS Canto II (BD ...
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bioRxiv - Immunology 2022Quote: Antibody staining was done in the dark for 30 minutes at 4°C in 30 μL of FACS buffer with antibodies and anti-CD16/32 (TruStain FcX™ PLUS; BioLegend, #156604; 1:100). Acquisition was done on a BD FACSCanto™ II or BD LSRFortessa (both BD Biosciences) ...
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bioRxiv - Microbiology 2022Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: BALF and post-digested lungs were centrifuged at 1500 rpm before resuspension in 100 μL of a 1:100 dilution of Zombie Yellow™ dye (Biolegend, San Diego, USA). After staining ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used in this study: anti-tubulin beta-3 conjugated to Alexa Fluor 488 (BioLegend; AB_2562669; mouse IgG2a, 1:200), Alexa Fluor 555 (BD PharMingen ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized with 0.1% Triton X-100 in PBS (5 min at RT) and immunolabeled with anti-HA (Biolegend, 901501; 1:500 in PBS) for 1 hr at RT ...
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bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: Other conjugated antibodies used in this study include the following: Alexa Fluor® 647 anti-Tubulin Beta 3 (1:1000, Biolegend, #657406, clone AA10), Alexa Fluor® 488 anti-Vimentin (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... peripheral blood and spleens were harvested from each mouse and the infiltrated primary cells were identified using flow cytometry by staining for human CD45 antibody (BioLegend; BLG-368522; 1:200).
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bioRxiv - Bioengineering 2024Quote: ... The sections were subsequently stained with a rat anti-human YAP antibody conjugated with PE (dilution: 1:500; clone W19260A; Biolegend, San Diego, CA, USA) for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with DAPI and the following antibodies and dilutions: Alexa Fluor® 488 anti-Vimentin (1:500, Biolegend, #677809, clone O91D3), anti-Ki67 (1:300 ...
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bioRxiv - Immunology 2022Quote: ... 4 °C) cells were stained with the following conjugated antibodies for 20 min at RT in the dark: CD3-APC (Biolegend, clone REA613, 1:200), CD11b-APC-Cy7 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... were blocked with 5% BSA and 5% goat serum in PBST (PBS/0.25% Triton X-100) for 30 min and then incubated with monoclonal 6E10 antibody (BioLegend, cat# 803001, 1:1000 dilution) overnight at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... the slices were transferred into 30% sucrose in water for >24 hours and then sub-sectioned on a sliding microtome to 15-30 μm for immunostaining using the following antibodies: mouse anti-HA (Biolegend catalog #901513, 1:1000) and rabbit anti-NeuN (Millipore catalog #ABN78 ...
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bioRxiv - Immunology 2023Quote: ... pancreatic islets were harvested from four 12-week-old female NOD mice and labelled individually with anti-TCRβ (BioLegend, allophycocyanin, H57-597, 1:300), anti-CD4 (BioLegend ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.2 g/L NaN3] for 15 min after which the grids were incubated on a 20 μl drop of primary antibodies—1:10 dilution of 6E10 (Biolegend, San Diego, CA, USA) and anti-αSyn MJFR1 (Abcam ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Isolated aortic single cells were stained with Fc receptor blocker (TruStain FcX anti-mouse CD16/32; 101319; BioLegend, San Diego, CA, USA, 1:100) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained with unconjugated rat anti-mouse lineage-specific antibodies (Ter-119, Mac1, Gr-1, B220, CD5, CD3, CD4, CD8, and CD127, Biolegend, San Diego, CA, USA), followed by incubating with Dynabeads anti-rat IgG (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 30 minutes at 4 °C using the following antibodies: Brilliant Violet 711™ anti-human CD4 (344648, BioLegend; 1 μL/well), APC anti-human CD3 (300412 ...
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bioRxiv - Cancer Biology 2023Quote: ... or the following antibody panel where the 488 and 555 channel markers were swapped: Alexa Fluor® 488 anti-Nestin (1:150, Biolegend, #656812, clone 10C2), Alexa Fluor® 555 anti-S100 beta (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... TCR-transduced CD8+ T cells were rapidly expanded for 5 days using irradiated feeder PBMCs and medium containing 60U/ml IL-2 (Proleukin, Novartis) and Ultra-LEAF™ Purified anti-human CD3 Antibody (clone OKT3, BioLegend 317326, 1:2000). After 5 days ...
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bioRxiv - Immunology 2023Quote: ... BV-421), CD62L (clone: MEL-14, PE), PD-1 (clone: 29F.1A12, PE-Cy7), CD107a (clone: 1D4B, APC) from BioLegend (San Diego, CA, USA), and NKG2D (clone ...
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Microbiology 2023Quote: ... washed once with PBS and stained for 30 min at RT in the dark with anti-CD4 antibody (PerCP-Cy5.5, Biolegend #317428, 1:50 in PBS) and eBioscience Fixable viability dye 780 (ThermoFisher Scientific #65-0865-14 ...
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bioRxiv - Cell Biology 2023Quote: ... Antibodies were combined in different panels comprising of an appropriate selection from the following: a 1:400 dilution of Alexa Fluor™ 750 anti-murine CD8 (BioLegend, Cat. No. 100766), a 1:400 dilution of Brilliant Violet™ 605 anti-murine CD4 (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... 800 μl of crosslinked chromatin was incubated with 25 μl Protein A Sepharose slurry (50%) in TBS and 1 μl of antibody against RNAP β (Biolegend, #663003, clone: NT63) for 90 minutes at room temperature while rotating ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were fixed with 2% neutral-buffered formalin diluted in FACS buffer following addition of Zombie Yellow fixable viability dye (BioLegend #423103, 1:200 in PBS).
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bioRxiv - Immunology 2021Quote: ... and stained by intracellular staining for 30 min at 4 °C using a cocktail of the following antibodies: IFN-γ PE (Biolegend, Clone W18272D,1:500), TNF Percp-Cy5.5(Biolegend ...
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bioRxiv - Developmental Biology 2021Quote: ... the cells were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then for 1 hour at 4°C in the dark with a 1:1 mix of rat anti-mouse CD41 (labelled with BV421 ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspensions were pre-incubated for 20 minutes at 4°C in the dark with unlabelled rat anti-mouse CD16/32 (BioLegend – 101320, 1 μg/million cells), then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then incubated with the following primary antibodies diluted in the incubation solution overnight: chicken anti-neurofilament NF-M (1:1000) (#822701, BioLegend, USA, previously #PCK-593P, Covance), rat anti-MBP (1:300 ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in PBS containing Zombie NIR™ dye (Biolegend; 1:200 in PBS; 100μl per well) and incubated at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in PBS containing Zombie NIR™ dye (Biolegend; 1:200 in PBS; 100μl per well) and incubated at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μm sections were sliced and stained with the murine version of the mAbs (1A8 muIgG2a-4m and 7C8 muIgG2a-4m) and compared to the anti-Aβ N-terminus positive control antibody (6E10, Biolegend, Cat. # #SIG39320, diluted 1:1000) and an IgG negative control antibody (used at a concentration of 5 μg/ml) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2022Quote: The following human cytokines were assessed by ELISA as previously described (10): human IFN-γ (capture MD-1 mAb; revealing biotinylated 4S.B3 mAb, Biolegend Cat#507502 and 502504, respectively), human IL-13 (capture JES10-5A2 mAb ...
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bioRxiv - Cancer Biology 2022Quote: Epithelial crypt cells from the ileum of Ki67-RFP ApccKO/cKO Villin-CreERT2 mice and microadenomas from Rosa26-tdTomato ApccKO/cKO Lgr5-EGFP-CreERT2 mice were stained with Pacific Blue™ (PB) anti-CD45 antibody (#103126, BioLegend, USA; dilution 1:200), PB-conjugated anti-CD31 (#102422 ...
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% Tween-20 and incubated overnight at 4°C in a humidified chamber with primary human anti-CD9 (Biolegend, catalog no. 312102, dilution 1:1000), human anti-CD63 (BD ...
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bioRxiv - Immunology 2024Quote: ... Yeast cells were washed two times with PBSF and subsequently stained for 30 minutes on ice with a cocktail of anti-HA APC antibody (Biolegend, Clone: 16B12, dilution 1:100), goat F(ab′)2 anti-human IgG PE (SouthernBiotech ...
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bioRxiv - Cancer Biology 2023Quote: ... The bone marrow was harvested from each mouse and the infiltrated CCRF cells were identified by staining for a human CD45 antibody (Biolegend; AB_2687375; BLG-368522; 1:200).
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bioRxiv - Developmental Biology 2024Quote: ... The cells were pelleted and resuspended in FACS buffer for staining with the following antibodies for 20 min at 4° C: Ter119-FITC (BioLegend, Catalog No. 116205, 1/100), EpCAM-PE (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then washed with FACS buffer and incubated with the following Lineage antibodies for 30 min at 4°C: CD11b Biotin (BioLegend, Catalog No. 101204, 1/1000), CD11c Biotin (BioLegend ...
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bioRxiv - Systems Biology 2023Quote: ... All iPSCs were stained in PBS with 5% FBS overnight at 4°C with the following antibodies and dilutions: Alexa Fluor® 647 anti-tubulin α (1:1000, Biolegend, #627908, clone 10D8), and Alexa Fluor ® 488 anti-Oct-4A (1:500 ...
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bioRxiv - Systems Biology 2023Quote: ... were used at a concentration of 1:100 then stained with anti-rabbit-PE or anti-mouse Pacific Blue secondary antibody (BioLegend® #406421, Life Technologies #P10993) for flow cytometry.
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bioRxiv - Immunology 2023Quote: ... and incubated with anti-BTN3A antibody (clone 20.1) or IgG1,κ isotype control (clone MOPC-21, BioLegend; or BM4-1, a gift from CSL Limited) at 5 µg/mL for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were resuspended in 10 μl of FACS Buffer and incubated with 1 μl of allophycocyanin (APC)-conjugated anti-CD46 antibody (BioLegend, clone TRA-2–10, 352405) for 30 min at 4°C with rotation ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 20 minutes at 4 °C using the following antibodies: Brilliant Violet 605™ anti-human interferon-gamma (IFN-γ) (506542, Biolegend; 1 μL/well). After being stained ...