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Citations for BioLegend :
7501 - 7550 of 7605 citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... freshly harvested PDX or EHS tumors were processed to single cells47 and stained with PE-conjugated anti-human CD298 PE (BioLegend; 1:50 dilution) and FITC-conjugated anti-mouse CD45 FITC (BioLegend ...
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bioRxiv - Neuroscience 2019Quote: ... The triangularis sterni muscle was dissected and incubated overnight (4°C) in anti-βIII-tubulin antibody conjugated to Alexa488 (BioLegend 801203; 1:200) and Alexa594-conjugated α-bungarotoxin (Invitrogen B13423 ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... Cells with successful PD-L1 deletion were isolated by two rounds of FACS sorting after O/N stimulation with 100 ng/ml IFNγ and staining with PD-L1-PE/Cy7 (clone 10F.9G2, Biolegend 124314, 1:200). Cells retaining PD-L1 expression were isolated simultaneously and served as controls.
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with Fc-blocking anti-CD16/32 anti-bodies followed by cell hashing antibody (1 μg/ml, BioLegend Cell Hashing A) (Stoeckius et al. ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACS buffer followed by PBS wash and stained with 100 μl of 1:1000 dilution of fixable Zombie Aqua (BioLegend, San Diego, CA) dye for 20 minutes at 4°C in dark ...
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bioRxiv - Immunology 2020Quote: ... and then incubated in the dark for 30 min at 4°C: Myeloid-lymphocyte common lineage panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend, San Diego, CA), CD45-Pacific Blue (clone ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 Satb1 cKO) of varying age 1-7 months by the LEGENDplex (13-plex) Mouse Th Cytokine Panel V02 (Biolegend, 740741; Lot B289245) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed once in PBS and then fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) before staining with PU.1-PE (BioLegend 7C2C34 cat #681307) or rat IgG2a-PE isotype (BioLegend RTK2758 ...
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bioRxiv - Cancer Biology 2022Quote: ... stained for 20 minutes on ice with the following antibodies at the following concentrations: anti-mouse CD49b 1:500 (#103511 Biolegend, San Diego, CA), anti-human CD49b 1:750 (#359310 Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... a sequential gating strategy was used to analyze cancer cells by staining with the following FACS antibodies (Figure S3F): CD45-Pacific Blue (BioLegend 103126, 1:100), CD31-Pacific Blue (BioLegend 102422 ...
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bioRxiv - Cell Biology 2022Quote: ... obtained single cell suspensions were stained with the fixable viability Zombie Aqua™ dye according with the manufacturer instructions (#423101; BioLegend, 1:500).
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bioRxiv - Neuroscience 2022Quote: ... human Aβ was immunodepleted from TgCM with the mouse antibody 6E10 (Purified anti-β-Amyloid, 1-16 antibody BioLegend Cat# 803004, RRID: AB_2715854) and Protein G Sepharose beads (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed with 15 mL of wash buffer (RPMI with 1% FBS) and the CD4+ T cells were enriched using MojoSort™ Mouse CD4 cell isolation (Biolegend, Cat#480006). The cells were stained and sorted as explained before in scRNAseq section as well ...
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bioRxiv - Immunology 2022Quote: ... T cells were added to anti-CD3 coated wells at 106 cells/mL in the presence of 1 μg/mL anti-CD28 (Biolegend, LEAF purified, 37.51), 100 U/ml IL-2 (gift from K ...
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bioRxiv - Immunology 2024Quote: ... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
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bioRxiv - Neuroscience 2024Quote: ... singlesuspension cells were stained at the cell surface in different panels with PerCP5.5-conjugated anti-CD45 (1:100, BioLegend Cat# 103132 (also 103131), RRID:AB_893340) ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with the following primary antibodies and dilutions: anti-Nestin (1:150, Biolegend, #656802, clone 10C2), anti-EGFR (1:300 ...
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bioRxiv - Microbiology 2024Quote: ... The panel of cytokines presented in Fig 5 was measured using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) (BioLegend, Cat# 740809) kit ...
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bioRxiv - Immunology 2024Quote: ... and incubated at 37°C for 30 min with either a 1/500 dilution of PE-labeled anti-6xHis tag mouse MAb (Biolegend, San Diego, CA) to detect VHHs or Alexa Fluor 647-labeled goat anti-human IgG (H+L ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
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bioRxiv - Cancer Biology 2023Quote: ... NKG2D and DNAM-1 surface receptors on NK cells were blocked utilizing 25 μg/ml of purified anti-mouse NKG2D (Biolegend, clone CX5, 130202), purified anti-mouse DNAM-1 (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25 ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Neuroscience 2023Quote: ... The following monoclonal antibodies were used for flow cytometry analyses at a dilution of 1/700: anti-CD45 (BioLegend, clone 30-F11, 103147), anti-CD11b (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... All cytokine antibodies were used at 1:100 dilution in 1x BD Perm/Wash buffer (anti-IFN-γ (APC/Cyanine7 or PE, Biolegend, #505849, #505808) and anti-TNF-ɑ (BV711 Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... was added to measure and gate on the % live HL-60 cells and anti-mouse/human CD11b PE (Biolegend, Catalog #101207, 1:50) was added to measure and gate on differentiated HL-60 cells ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... Cells were incubated with a TotalSeq-C antibody cocktail as well as hashtag antibodies to allow for post sequencing differentiation of distinct expanded ILC subsets(BioLegend, Supplemental Table 1) for 30min ...
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bioRxiv - Neuroscience 2023Quote: ... Extracellular deposits of Aβ peptide were immunostained with the mouse anti-β-amyloid 17-24 antibody (1:5000, clone 4G8, BioLegend, Koblenz, Germany). Alpha-synuclein pathology was detected with the anti-syn-1 antibody (1:2000 ...
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bioRxiv - Bioengineering 2023Quote: ... coated glass bottom chamber and then labeled with 1ugꞏml−1 of Alexa Flour (AF) 488 conjugated anti-human CD45 antibodies (Biolegend, Cat# 304017, clone HI30) in 2% BSA in PBS for 20 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mg of protein G agarose beads were crosslinked to 5 μL of mouse anti-HA antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) using an effective concentration of 6.5 mg mL-1 DMP ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used to validate siRNA knock-down in fibroblasts: anti-α2-spectrin (mouse IgG2b clone D8B7, Biolegend #803201, 1:1000), anti-β2-spectrin (BD Biosciences #612563 ...
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bioRxiv - Cell Biology 2023Quote: ... quantification of PG release from cartilage explants was performed using the dimethylmethylene blue assay (36, 37) and exposure to sialidase (1.9 U/mL) and interleukin-1 beta (Cat #. 300-01A; BioLegend, San Diego, CA, USA) (10 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were suspended at 3×106 cell/ml and incubated for 30 min at 4C with 1:100 dilutions of the following antibodies: [APC dump channel: Ter-119 (Biolegend cat no. 116212), CD45 (Biolegend cat no ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the NK-92 staining panel: anti-CD107a (LAMP-1) FITC (BioLegend catalog #328606, clone H4A3), anti-CD56 (NCAM ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were pretreated with 100 U/mL IFN-γ for 24h and stained with 1:1000 H2kb-SIINFEKL Ab (BioLegend,Clone:25-D1.16). GFP-expressing cell lines were used for GFP compensation ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended by mild vortexing and 50 ul of 2.5 µg/ml final (meaning 1/200 dilution of the stock) Fc-block (anti-mouse anti-CD16/32 antibodies, TruStain FcX from Biolegend, San Diego, CA) were added with the following addition of 1/200 dilution of antibodies for surface marker staining and Aqua Live/Dead dye (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... non-specific Fc receptor binding was blocked using 1:100 dilution of TruStain FcX anti-mouse CD16/32 antibody (BioLegend, RRID:AB_1574975, Cat#101320). 5 min after Fc blocker treatment ...
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bioRxiv - Physiology 2024Quote: ... collected into FACS tubes (Falcon, cat no. 352052) and incubated with the following antibodies: AF700-labeled CD54 (1:100, BioLegend, cat no. 353126) and PE/Cy7-labeled CD62E (1:100 ...
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bioRxiv - Immunology 2020Quote: ... ASC was stained using a primary antibody anti-ASC (polyclonal anti-PYD ASC, Santa Cruz or monoclonal anti-CARD ASC, Biolegend; 1:1000 dilution) or anti-Luciferase (Abcam ...
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bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized with ice-cold methanol at −20°C for at least 20 min and incubated with anti-Pol II antibody (Biolegend, 8WG16 at 1:200), anti-PABPC antibody (Abcam ab21060 at 1:200 for mouse cells and Santa Cruz sc32318 at 1:25 for human cells ...
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bioRxiv - Cell Biology 2021Quote: ... and the cell pellet was resuspended in 1 volume (V) CnT-PR-MSC-XF-HC before adding 3V of 1x Red Blood Cell Lysis Buffer (Biolegend, San Diego, United States). After 10 minutes of incubation at room temperature another 10V of CnT-PR-MSC-XF-HC were added and the cell suspension was centrifuged ...
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bioRxiv - Microbiology 2021Quote: Concentrations of cytokines and chemokines present in the serum from SARS-CoV-2 infected AGMs were quantified using a multiplex bead-based assay (1:4 dilution)-the LEGENDPlex Non-Human Primate Cytokine/Chemokines 13-plex (BioLegend, San Diego, CA USA). Analytes detected by this panel are the following ...
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bioRxiv - Cancer Biology 2022Quote: ... Human tissues were incubated for 25 min at 4 °C with the following primary antibodies: CD24-PE (BioLegend, clone ML5, Cat, #: 311105, 1:100), CD44-FITC (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... Staining for human immunoglobulins was performed in 100 μL with 1:50 (v/v) of the detection antibodies: anti-human IgM Brilliant Violet 650 (clone: MHM-88, Biolegend® Cat. No. 314526), anti-human IgG PE/ Dazzle™ 594(clone ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in PBS containing Zombie Violet dye (Biolegend; 1:200 in PBS; 100μl per well) and incubated at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... at a dilution of 1:200 for 30 min at room temperature followed by incubation with horseradish peroxidase streptavidin at a dilution of 1:125 (405210, BioLegend, San Diego, CA, USA) for 30 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... cells were resuspended in PBS complemented with 2% fetal bovine serum and stained with FITC anti-human CD45 antibody (BioLegend #304006CD45, 1:200 dilution) and PE anti-human EPCAM antibody (Miltenyi Biotech #130-113-264 ...