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Citations for BioLegend :
7251 - 7300 of 7605 citations for Glutathione Reductase Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Cells were pelleted by centrifugation and then resuspended in 100 μL FACs buffer (PBS with 10 % FCS) with PE-conjugated anti-CD61 (RRID:AB_313084, Biolegend, 104307, 1:200) and Alexa Fluor 647-conjugated anti-SSEA1 (RRID:AB_1210551 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with the following primary antibodies overnight at 4 : mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302) + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked using PBS/1% BSA/0.5% Tween-20 and incubated with monoclonal mouse FITC anti BrdU (Biolegend, clone 3D4) at 1:50 dilution for 20 minutes ...
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bioRxiv - Genomics 2020Quote: ... and resuspended in a solution of FACS buffer with a 1:100 dilution of PE anti-human B2-microglobin (Biolegend 316306). Cells were incubated for 30 mins in the dark at 4 °C ...
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bioRxiv - Genomics 2019Quote: ... Isolated nuclei were labeled with an Alexa Fluor 647 anti-GFP antibody (338006, clone FM264G, 1:25, BioLegend, San Diego, CA) for 30 minutes and 2 μg/ml DAPI immediately prior to sorting ...
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bioRxiv - Immunology 2019Quote: ... The cells were incubated with the following antibodies (1:100) for 30 minutes at room temperature: Alexa Fluor 488 anti-mouse CD25 (Biolegend, 102017), PE/Cy7 anti-mouse/human CD44 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... The following antibodies were added (1:100) to each sample and incubated for 30 minutes at room temperature Alexa Fluor 700 anti-mouse CD8a (Biolegend, 100730), PE/Dazzle-594 anti-mouse CD4 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... anti-CD3 (clone OKT3, BV650), anti-CD4 (clone OKT4, BV785), anti-PD-1 (EH122.2H7, BV711), anti-CD27 (O323, BV605) from Biolegend (London, UK); anti-CD45 (clone 2D1 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3–8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3-8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... or double knockout cells were harvested and washed with PBS containing 1% bovine serum albumin (BSA) and incubated with 5 μL of PE-conjugated EphA2 antibody (Biolegend, SHM16) in 50 μL PBS containing 1% BSA for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2019Quote: ... 105 resting NK cells were mixed at 1:1 with 221 cell lines or peptide loaded target cells in presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). NK cells were then stained with mAbs to identify subsets based on the expression of KIR2DL1/S1 (Beckman Coulter ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and stained in 100 μl for 30 minutes on ice using an anti-mouse IgG-Brilliant Violet421 secondary antibody at 1:90 (BioLegend, 405317). Cells were again washed twice ...
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bioRxiv - Immunology 2021Quote: ... Samples were then washed twice with 1 mL of Intracellular Staining Perm Wash buffer (Cat: 421002, BioLegend, San Diego, CA, USA); after the second wash ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells are permeabilized with 0.1% Triton-X 100 for 10min at RT and stained with anti-vimentin (1:200, Biolegend, CA, USA) for 30 min in PBS containing 5% normal goat serum (RT) ...
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bioRxiv - Cancer Biology 2021Quote: Wildtype RMC219 and RMC2C cells were harvested and 1 x 106 cells were resuspended in buffer A (PBS 1X, EDTA 2mM, inactivated FCS 1%) and 5uL of Human TruStain FcX (Biolegend, 422301) was added for 10 min at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... Antigen-independent activation-marker expression of CAR-T cells was evaluated by antibody staining for CD137 (PE/Cy7, clone 4B4-1, BioLegend #309818) and CTLA-4 (PE/Cy7 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting was repeated on cultured CAFs and PVL cells using the previously described experimental conditions with anti-PDGFRα (1:100; BioLegend #323507), anti-CD146 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-DnaK 4RA2 at 1□:□10,□000 dilution (Enzo, ADI-SPA-880) and HRP Goat anti-mouse IgG (BioLegend, 405306) at 1□:□10,□000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were collected and stained using APC/Fire anti-CD4 and APC anti-CD8 (Supplemental Table 1) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supplemental Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... CD59 (PE, 143103), PD-1 (BV605, 135220), CD44 (BV711, 103057), CD69 (PE-Cy7, 104512), IFN-γ (APC, 505810) (all from Biolegend). The Foxp3 Fix/Perm Kit was used for intracellular staining (421403 ...
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bioRxiv - Microbiology 2021Quote: We performed western blotting as in (Polino et al., 2020) with primary antibodies mouse anti-HA diluted 1:1000 (clone 16B12; Biolegend #901501), rabbit anti-PfAldolase diluted 1:2000 (abcam #ab207494 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking of Fc receptors was performed by incubation at 4°C for 20 minutes with an unlabelled anti-CD16/32 (1 μg/million cells; BioLegend – 101320). The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin ...
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bioRxiv - Systems Biology 2021Quote: Single nuclei from each PDX condition were labeled with 1 μl condition-specific hashtag oligonucleotide-labeled antibodies (BioLegend, TotalSeq A0541-A0545) according to manufacturer’s protocol prior to pooling and loading on a single lane of the 10X Chromium v2 system ...
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bioRxiv - Microbiology 2019Quote: ... Cells were harvested using Trypsin-EDTA and resuspended in FACS buffer (PBS containing 10% FBS and 2mM EDTA) and stained with anti-CD300lf PE clone Tx70 (1:100; BioLegend, #132704) or anti-CD300ld PE clone Tx69 (1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... VDR overexpressing SKOV-3 and ECC-1 cells were processed and stained with PDL1-BV605 antibody (Biolegend Inc, Catalog number:124321). VDR expressing SKOV-3 and ECC-1 cells ...
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bioRxiv - Cell Biology 2021Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...
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bioRxiv - Cell Biology 2021Quote: ... Mac-1-PE-Cy7 and FcγR-PE (eBioscience, 12-0161-83) for myeloid differentiation, or Mac-1-APC (eBioscience, 17-0112-82) and CD19-PB (BioLegend, 115523) for lymphoid differentiation on OP9 cells ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was removed and approximately 106 cells were resuspended in FACS buffer containing an anti-HA AF488 conjugate (1:1000, BioLegend, 901509) and an eFluor 780 fixable viability dye (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once in Cyto-Fast Wash Buffer and incubated in Cyto-Fast Wash Buffer containing an anti-HA AF647 conjugate (1:1000, BioLegend, 682404) and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M ...
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bioRxiv - Molecular Biology 2022Quote: ... Whole-lung suspension was then repelleted and resuspended in SB + 1:50 TruStain FcX™ antibody (anti-mouse CD16/32, BioLegend) for a 10-minute blocking period at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for removal of mouse stromal cells included: anti-mouse H-2Kd-biotin (BioLegend, 116604, clone SF1.1, dilution 1:20) and anti-mouse Cd45-PE/Cyanine5 (BioLegend 103110 ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Cancer Biology 2022Quote: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance ...
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bioRxiv - Neuroscience 2020Quote: ... The following antibodies were then added to cell suspensions at 1:200 concentrations: CD11b-PE (BioLegend Cat#: 101208; Clone: M1/70), CD45-APC/Cy7 (BioLegend Cat# ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times with PBS-T (PBS with 0.1% Tween-20) and 50μl of mouse IgG-specific secondary antibody (BioLegend #405306, 1:10,000) diluted in dilution solution added to each well ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies were added at the concentration of 1:50 and incubated on ice for 45 minutes: APC/Cy7 Cd45 (Biolegend #103116), APC Pecam1 (Biolegend #102410) ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellets washed twice with 10% FCS in PBS (100μl/well) and then resuspended in 10% FCS in PBS (100μl/well) containing APC/Cyanine7 Goat anti-mouse IgG (Biolegend; 1:250) and incubated on ice for 30mins ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMCs were thawed and washed twice with 10 ml of FACS buffer (1 x PBS containing 2% FBS and 1 mM EDTA) and resuspended in 100 μl of 1x PBS containing Zombie UV live/dead dye at 1:200 dilution (BioLegend, 423108) and incubate at room temperature for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... with the following modifications: Approximately 1 million cells per sample were resuspended in 95 ul of cell staining buffer (Biolegend, USA) with 5 ul of Fc receptor Block (TrueStain FcX ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were then treated with 25 μl of APC-antiHA antibody with 1:75 dilution (~0.07 μg/well, BioLegend catalog # 901523) for 1 hour on ice in the dark ...
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bioRxiv - Biophysics 2019Quote: ... siRNA mediated knockdown was further confirmed by imaging cells labelled with Alexa Fluor 488 anti-human FcƐRIɑ antibody AER-37 (CRA-1) (BioLegend Products, Singapore).
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bioRxiv - Immunology 2019Quote: ... Antibody coated cells were mixed with resting NK cells in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Cells were then stained with mAbs to KIR2DS4 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2019Quote: ... Purity of isolated cells was validated by FACS by using the cell surface markers EpCAM-APC (Biolegend, 118213, 1:50 dilution) and CD45-PE (Biolegend ...
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bioRxiv - Cell Biology 2019Quote: ... blocked with 1 mg/mL human IgG (Privigen, Behring CSL) and stained with mAb 7–239 α-Siglec-1-PE or matched isotype-PE control (Biolegend) at 4°C for 30 min ...
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bioRxiv - Genomics 2019Quote: ... The following antibodies were added (1:100) to each sample and incubated for 30 minutes at room temperature: Alexa Fluor 700 anti-mouse CD8a (Biolegend, 100730), PE/Dazzle-594 anti-mouse CD4 (Biolegend ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with the following antibodies in isolation or in combination for 30-60 minutes in the dark at 4°C: ICAM-1 PE (BioLegend, #353106), CCR6 PE/Dazzle (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were processed at the same time using the purified mouse monoclonal anti-beta-amyloid 1-16 antibody (6E10) (Biolegend, 803001) at 1/500 for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with the following primary mouse antibodies (1:100 dilution) for 30 minutes at 4°C: CD45-BV750 (clone 30-F11, Biolegend, 103157), CD31-PE-Cy5.5 (clone MEC 13.3 ...