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Citations for BioLegend :
601 - 650 of 7409 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Primary human CD4+ T cells from CTLA4-deficient patients or healthy controls were purified from 5 million frozen PBMCs using a biotin labeled anti-human CD4 antibody (OKT4; BioLegend; 1:20) followed by magnetic separation with anti-biotin microbeads (Miltenyi Biotic ...
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bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
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bioRxiv - Immunology 2024Quote: ... Human CD4+ T cells (4X105/well in 96-well plate) were activated with plate-coated αCD3 (5µg/ml, Biolegend) and αCD28 (10µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-human KIT-PE (BioLegend 313204, clone 104D2, dilution 1:50). After staining ...
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bioRxiv - Cell Biology 2022Quote: ... Zombie Violet™ Fixable Viability Kit (1:100, BioLegend, catalog number 423114) was used to remove dead cells for 20 min at room temperature according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and viability stain (Zombie Aqua™ Fixable Viability Kit, Biolegend, 1:500) at RT for 10 min in the dark ...
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bioRxiv - Immunology 2023Quote: ... DLD-1 cells were labeled with CFSE Cell Division Tracker Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: The LEGENDplex™ Human Inflammation Panel 1 kit (BioLegend, San Diego, USA) was utilized to measure cytokines and chemokines ...
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bioRxiv - Immunology 2021Quote: ... cell-free supernatants were collected followed by ELISA to detect IL-10 and TGFβ using capture and detecting antibodies (Biolegend). Other M2 phenotype characteristics including the cell surface expressions of CD206 ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ or GM-CSF release in the cell culture supernatant was measured using ELISA according to the manufacturer’s protocol (Sanquin and BioLegend, respectively).
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bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2020Quote: ... The supernatants were collected and the amount of IL10 in the supernatants were measured using the Human IL10 ELISA MAX (Biolegend) kit according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The samples were then flash frozen and defrosted for 4 rounds to lyse the cells and used on an IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Immunology 2020Quote: ... Cytokine content of cell free supernatants was determined using standard sandwich ELISA according to the manufacturer’s instructions (IFN-γ and GM-CSF; Sanquin and Biolegend). Some target cells were pre-incubated for 2 days in the presence or absence of UGCG inhibitors.
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bioRxiv - Immunology 2022Quote: ... TNF and IL-2 in the supernatant of in vitro activated cells and murine IL-10 in the serum were determined using ELISA MAX Deluxe Set (BioLegend) according to manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: ... Secreted levels of IFN-β were measured from BMDM supernatants twenty-four hours following the start of the infection following the Sandwich ELISA protocol published by BioLegend. Anti-IFN-β capture antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in RLT buffer and supernatants were collected for analysis using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1.
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bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and GM-CSF (purified anti-mouse GM-CSF; biotin anti-mouse GM-CSF) ELISA antibodies and standards were purchased separately from Biolegend.
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bioRxiv - Immunology 2023Quote: ... Tgf-β concentration was measured using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1 ...
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bioRxiv - Immunology 2023Quote: ... Serum samples were assessed for titers of different antibody isotypes using LegendPlex Mouse Immunoglobulin Isotyping Panel and IgE ELISA (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... TNF-α production was quantified in the supernatant of RAW 264.7 cells using a sandwich ELISA Max Deluxe Set (Biolegend) according to the manufacturers protocol.
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bioRxiv - Immunology 2022Quote: ... Cells were plated in a 24-well plate at a concentration of 500,000 cells/mL on either plate-bound α-CD3ε (2 μg/mL; 145-2C11; BioLegend) and soluble α-CD28 (1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with FACs buffer (1X PBS, 1% FBS) and fixed at room temperature for 20 minutes with Intracellular Staining Fixation Buffer (BioLegend, San Diego, CA). Cells were washed with 1X Cyto-Fast™ Perm Wash solution (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... and were stained at 20 μl of FACS buffer per 1×106 cells containing the first set of following antibodies (all from Biolegend, unless specified otherwise): biotinylated hematopoietic lineage antibodies against NK1.1 (clone PK136) ...
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bioRxiv - Cancer Biology 2022Quote: ... stained for 20 minutes on ice with the following antibodies at the following concentrations: anti-mouse CD49b 1:500 (#103511 Biolegend, San Diego, CA), anti-human CD49b 1:750 (#359310 Biolegend) ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells that have been incubating with conditioned media were carefully transferred to the CD3-coated plates and further stimulated with 1 µg/mL anti-CD28 (BioLegend, clone: CD28.2) for another 48 hours ...
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bioRxiv - Microbiology 2021Quote: ... antibody and 20 ng/ml IL-2 (Biolegend #589106) for 3 days ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/mL of recombinant mouse SCF (579706; Biolegend) and recombinant human TPO (763706 ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng/mL recombinant human thrombopoietin (hTPO) (BioLegend #597402), DNase (100 μg/mL final concentration) ...
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bioRxiv - Microbiology 2023Quote: ... the plates were incubated with TMB substrate (BioLegend) for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... they were stained with Zombie Violet™ Fixable Viability Kit (1:1000, BioLegend) for 30 minutes on ice during secondary antibody staining ...
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bioRxiv - Developmental Biology 2022Quote: ... were incubated with Zombie Aqua™ Fixable Viability Kit (Biolegend, #423101, 1:1000) for 15 min at room temperature ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... PE/Cy7-conjugated anti-mouse CD117 (c-Kit) antibody (1:200, Biolegend, 105814) and FITC-conjugated anti-mouse CD9 antibody (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... To exclude dead cells Zombie Aqua Fixable Viability Kit (#423102, 1:100, BioLegend) was added ...
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bioRxiv - Cancer Biology 2022Quote: ... the antibody or IgG1 excess was rinsed and pellets were resuspended in APC-conjugated Annexin V at 1:20 in Annexin V buffer solution plus Zombie Violet dye at 1:400 (all antibodies and probes are from Biolegend, San Diego, CA, USA). Samples were transferred into FACS tubes and incubated for 20 minutes at room temperature protected from light prior to their analysis by FACS Canto II (BD ...
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bioRxiv - Immunology 2022Quote: ... 4 °C) cells were stained with the following conjugated antibodies for 20 min at RT in the dark: CD3-APC (Biolegend, clone REA613, 1:200), CD11b-APC-Cy7 (Biolegend ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.2 g/L NaN3] for 15 min after which the grids were incubated on a 20 μl drop of primary antibodies—1:10 dilution of 6E10 (Biolegend, San Diego, CA, USA) and anti-αSyn MJFR1 (Abcam ...
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bioRxiv - Immunology 2023Quote: ... pDCs were gated for flow cytometry with 1:20 dilution commercial mouse anti-human BDCA-2-Alexa488 (stock 200 ug/mL, BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD123 (IL-3)-PE (stock 200 ug/mL ...
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bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... TNFα and IL-23 expression in the culture medium was examined using an ELISA (Invitrogen for TNFα Biolegend for IL-23).
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... that were pulsed with epitope (10 ng/mL) was collected and IFN-γ production was measured with an enzyme-linked immunosorbent assay (ELISA, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend, 10ug/ml) or with IL-12 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 ng/ml mouse EGF (BioLegend, San Diego, CA, USA), 10 µg/ml bovine insulin (Sigma-Aldrich by MilliporeSigma) ...