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Citations for BioLegend :
751 - 800 of 7409 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... cell culture plates were pre-coated with anti-mouse CD3 antibody (Biolegend) (1 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... in the presence of plate- bound anti-CD3 (5 μg/mL, Biolegend), CpG (10 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed and developed with 50 µl TMB (Biolegend, UK); after 5 minutes the reaction was stopped with 50 µl 1M sulfuric acid and absorbance measured at 450 and 630 nm ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated on a plate coated with anti-CD140a (#135902, Biolegend) for OPC selection ...
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bioRxiv - Immunology 2024Quote: ... ELISPOT plates were coated with capture α-IFNγ (clone R4-6A2, Biolegend) and subsequently blocked ...
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bioRxiv - Cancer Biology 2024Quote: ... plates were coated with 10 μg/ml anti-CD3 (145-2C11, Biolegend) for 2 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: IL-1 cytokine concentrations in supernatants from cultured BMDMΦ were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (31,50).
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bioRxiv - Immunology 2021Quote: ... the isolated B cells were incubated with 1 μl Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107) per 1 ml cell suspension for 30 min on ice ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% BSA and 0.07% NaN3 in PBS) with additional Zombie Aqua™ (1:1,000 Fixable Viability Kit, BioLegend) to stain for vital cells ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Immunology 2020Quote: ... BMDMs were generated by culturing BM cells with rmM-CSF (20 ng/ml, BioLegend) and BMDCs were generated with rmGM-CSF (20 ng/ml ...
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bioRxiv - Immunology 2019Quote: ... then incubated for 20 minutes in 50 ul of Fixation/Permeabilization solution (BD Biolegend). Cells were washed three times in Perm/Wash buffer (BD Biolegend) ...
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bioRxiv - Neuroscience 2020Quote: ... Cell pellet was fixed for 20 minutes in the dark in fixation buffer (Biolegend) followed by permeabilization with permeabilization buffer (Biolegend) ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) (Biolegend, San Diego, CA) and 10 ng/ml IL-4 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... and 20 μg/mL 7-AAD solution (7-amino-actinomycin D, Biolegend, 650 nm) for 15 min at room temperature in dark ...
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bioRxiv - Cancer Biology 2022Quote: ... and with or without anti-IL-6 antibody (20 μg/ml, Biolegend Cat#504512). Cells were then harvested from plates ...
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bioRxiv - Cell Biology 2020Quote: ... DNA content was stained by 20 min incubation in 0.5 μg/ml DAPI (BioLegend) in Perm/Wash at RT ...
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bioRxiv - Biochemistry 2021Quote: ... and the other was incubated with anti-flag affinity beads (20 μL slurry, BioLegend) for 2-3h at 4 ° C ...
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bioRxiv - Bioengineering 2022Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend, 576406). Successful differentiation was confirmed by flow cytometry staining with antibodies against macrophage markers ...
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bioRxiv - Microbiology 2021Quote: ... and vascular markers of interest conjugated to A647 (CD31 (Biolegend, used at 20 μg), Ephrin B2 (R&D systems ...
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bioRxiv - Cancer Biology 2022Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating ...
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bioRxiv - Immunology 2023Quote: ... Recombinant Mouse GM-CSF (carrier-free; 20 ng/ml; BioLegend, San Diego, California, USA), 2 mM L-glutamine (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend). Intracellular staining was performed for cytokines using antibodies for IL-5 ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained for 20 min in BSS with annexin V (APC #640920 Biolegend) at room temperature in the dark ...
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bioRxiv - Immunology 2023Quote: ... cell pellets were resuspended in 20 μL FACS buffer with 0.25 μg FcBlock (BioLegend) for five minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating ...
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bioRxiv - Immunology 2019Quote: ... Plates were washed and incubated with goat IgG anti-mouse IgA-HRP (BioLegend). Plates were washed and developed in the dark using TMB substrate ...
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bioRxiv - Microbiology 2020Quote: ... The cell culture plates were pre-coated with anti-mouse CD3 antibody (Biolegend) (1 μg/ml) ...
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bioRxiv - Immunology 2020Quote: ... T cell activation was performed using plate-bound α-CD3 (clone OKT3, Biolegend) and α-CD28 (clone 9.3 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were restimulated on plates coated with anti-CD3 and anti-CD28 (BioLegend). After 48 hours of restimulation ...
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was provided by plate-bound αCD3 (clone HIT3a, BioLegend, cat#300313) at 1μg/mL unless otherwise specified ...
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bioRxiv - Immunology 2022Quote: ... plates were washed and incubated with 100 μL/well TMB-substrate (Biolegend/ITK). The substrate reacted approximately 8 minutes ...
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bioRxiv - Immunology 2022Quote: ... T cells were activated with plate-bound anti-CD3ε (clone 145-2C11; Biolegend) and 0.5 µg/ml of soluble anti-CD28 (clone 37.51 ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/ml anti-mouse-IgM or IgG (Biolegend) in NaHCO3 0.1 M pH 9.6 ...
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bioRxiv - Bioengineering 2024Quote: ... and then activated by pre-coated plate-bound anti-CD3 (1μg/ml; Biolegend) and soluble anti-CD28 ((1μg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... Plates were then washed with PBS before the addition of 1x streptavidin (Biolegend) in PBS + 0.5% BSA (w/v ...
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bioRxiv - Systems Biology 2019Quote: ... and IL-6 secreted protein level in cell supernatant from 1×106 human MoDM’s was performed by Legend Plex multi-analyte assay kit (BioLegend) according to manufacturer’s instruction ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... and lineage-negative cells (Lin-) were analyzed for c-Kit (clone 2B8; eBioscience) and Sca-1 (clone D7; BioLegend) expression to discriminate Kit+ Sca-1- (KL ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by permeabilization for 1h at −20°C with True-Phos™ Perm Buffer (Biolegend) as per manufacturer’s specifications ...
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bioRxiv - Immunology 2021Quote: ... 20 µL diluted peptide and 20µL 1µg/mL UV-sensitive peptide HLA-A2 monomer (BioLegend) were added into 96-well plates and mixed well by pipetting up and down ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were collected and fixed for 20 minutes at RT with fixation buffer (Biolegend 420801), then samples were run on a BD LSRII (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... 20 µl/ml mouse serum and 10 µl/ml Fc block (CD16/32) (Biolegend, CA). Plates were incubated for 2 hrs at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Tfr cells were resuspended at 20 × 106 cells/ml in CITE-seq staining buffer (BioLegend) and incubated with Human TruStain FcX Fc Blocking reagent for 10 min at 4°C to block nonspecific antibody binding ...
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bioRxiv - Immunology 2023Quote: ... single-cell suspensions were incubated for 20 minutes with 50nM of MitoSpy Orange CMTMRos (BioLegend) and 50nM of MitoTracker DeepRed FM (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml recombinant mouse (rm)-SCF (Miltenyi) and 20 ng/ml rm-TPO (BioLegend), in the presence or absence of 300 pg/ml rm-IL21 (R&D Systems) ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 ng/ml recombinant mouse (rm)-SCF (Miltenyi) and 20 ng/ml rm-TPO (BioLegend), in the presence or absence of 300 pg/ml rm-IL21 (R&D Systems) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed for 20 min in 150 μl 4% paraformaldehyde (BioLegend; cat. no., 420801) at 4 °C in darkness before a 30-min permeabilisation step (eBioscience ...
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bioRxiv - Genomics 2024Quote: ... expanded CD34+ cells were cultured in basal medium with 20 ng/μl GM-CSF (Biolegend) and 1 x CC100 (StemCell Technologies ...
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bioRxiv - Immunology 2024Quote: ... the cells underwent a 20-minute subsequent stain at room temperature with Streptavidin-PE (Biolegend), followed by two additional washes ...