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Citations for BioLegend :
451 - 500 of 7409 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... and IL-10 in culture supernatants were measured using ELISA Max Deluxe were from Biolegend® (CA ...
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bioRxiv - Neuroscience 2021Quote: ... then analyzed for IL-1β using an IL-1β ELISA (BioLegend, 437004, San Diego CA) according to the manufacturer protocol.
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bioRxiv - Immunology 2021Quote: ... IL-17 and IFNγ in supernatants were assayed by ELISA (Biolegend or R&D systems). For data output cell number and ELISA were normalized per 1,000 of each APC subset used for co-culture.
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bioRxiv - Immunology 2021Quote: ... and supernatants were harvested for IL-6 and IL-1β ELISA (Biolegend, San Diego, CA). BMDCs were also utilized in the MLR and in vitro T cell differentiation assays.
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bioRxiv - Immunology 2023Quote: ... and cytokines (IFN-y and IL-5) were measured by ELISA (Biolegend. San Diego, CA). For intracellular cytokine determination ...
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bioRxiv - Synthetic Biology 2023Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFNɣ by ELISA (BioLegend).
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... ELISA assay to measure VEGFA levels was performed according to the manufacturers’ protocol (Biolegend, 430204) using the lung cancer cell supernatant.
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bioRxiv - Cell Biology 2021Quote: ... followed by the addition of 1:20 (1:40 final) primary antibodies against LAMP1 (AF488- or BV605-conjugated; BioLegend cat. no. 328610 and 328634), and/or TFRC (FITC-conjugated ...
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bioRxiv - Immunology 2020Quote: ... Coated plates were washed and cells were plated with 1 µg/ml soluble αCD28 antibody (37.51, Biolegend). Bone marrow-derived dendritic cells (BMDC ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-PCNA antibody clone PC10 conjugated to Alexa Fluor 647 (BioLegend, 307912, San Diego, CA, 1/20 dilution), anti-Ki-67 antibody D3B5 (Cell Signaling Technology ...
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bioRxiv - Immunology 2019Quote: ... cells were then incubated with streptavidin-BV711 for 20 minutes at 4°C (Biolegend 405241; Dilution 1:200).
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bioRxiv - Genomics 2021Quote: The following fluorophore-conjugated primary antibodies were used: CD3-Brilliant Violet 421 (1:20, clone 17A2, BioLegend 100227) and NK1.1-AlexaFluor 488 (1:200 ...
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bioRxiv - Immunology 2023Quote: ... cells were centrifuged and resuspended in 1 ml of −20°C cold TruePhos™ Perm Buffer (BioLegend, USA) and incubated at −20°C for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Surface markers were subsequently stained on ice for 20 minutes: anti-mouse CD3-PE (1:200, 17A2, Biolegend), anti-mouse CD4-APC (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 million cells were incubated for 20 min at room temperature with Zombie viability dye (Biolegend ref 77143). Then cells were incubated in PBS + 10% FBS to block Fc receptors ...
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bioRxiv - Immunology 2023Quote: ... and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL, BioLegend, San Diego, CA, USA), which has been reported as a marker for juvenile B cells ...
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bioRxiv - Biochemistry 2020Quote: ... CD117 (c-Kit) (Biolegend, clone 2B8, APC, 1:50), CD127 (IL-7Rα ...
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bioRxiv - Cell Biology 2021Quote: ... About 0.3 million cells in 37.5 μl CSM were loaded in each well of a 96-well plate of the Human Cell Surface Marker Screening (phycoerythrin [PE]) Kit (Biolegend). Each well contained 12.5 μl of diluted PE-conjugated antibody in CSM ...
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (20 ng/ml; BioLegend), IL-4 (20 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... 20 ng/mL IL12 (BioLegend, #573002) and 10 ng/mL anti-IL4 (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... sCD14 (20-200 ng/ml, Biolegend) was added on macrophages for intracellular signaling measurements (15 min ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/mL IL-12 (Biolegend), 20 ng/mL IL-23 (R&D Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... 20 ng/mL IL-3 (Biolegend), 50 ng/mL IL-6 (Biolegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 ng/mL of IL23 (BioLegend) was added to the culture on Days 2 and 3 ...
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bioRxiv - Developmental Biology 2024Quote: ... and 20 ng/ml hFGF (Biolegend) for 7 days ...
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bioRxiv - Developmental Biology 2024Quote: ... and 20 ng/ml hFGF (Biolegend) for 6 days ...
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bioRxiv - Immunology 2022Quote: ... IL-1α and IL-6 were quantified with Mouse IFN-γ ELISA MAXTM Deluxe Set (Biolegend), Mouse IL-1α ELISA MAXTM Deluxe Set (Biolegend ...
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bioRxiv - Synthetic Biology 2020Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFN◻ by ELISA (BioLegend). All assays were performed in triplicate and average IFN◻ production was plotted with standard deviation.
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bioRxiv - Synthetic Biology 2021Quote: ... and the cell lysates were assessed on a human IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Cancer Biology 2022Quote: ... using ELISA MAX Deluxe Set human (IFNγ #430104, IL-2 # 431804, and IL-6 #430504, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cytokine secretion in the supernatant was evaluated by ELISA MAX Deluxe Set Human TNF-α (BioLegend), ELISA MAX Deluxe Set Human IFN-γ (BioLegend) ...
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bioRxiv - Immunology 2023Quote: IL-2 concentrations in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) from BioLegend. For the CRISPR screen ...
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bioRxiv - Synthetic Biology 2024Quote: ... cell supernatants were collected to monitor IFNG protein expression by ELISA according to manufacturer protocols (BioLegend). Antibodies used ...
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bioRxiv - Immunology 2024Quote: ... and IL-1β were carried out using ELISA MAXTM Standard Set (Biolegend, San Diego, CA, USA). In brief ...
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bioRxiv - Immunology 2023Quote: ... sorted TN and TEMRA cells were stimulated with plate-bound anti-human CD3 (OKT3; 1 μg/ml; BioLegend) and anti-human CD28 (CD28.2 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.02% Tween-20 in PBS) and incubated with 1 µg of a unique TotalSeq-A nuclear hashing antibody (Biolegend) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained for 20-30 minutes on ice with 1:200 anti-CD11b-BV711 (Clone M1/70; Biolegend) prior to analysis by flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Genomics 2019Quote: ... The resuspended cells were incubated for 20 min at 4 °C with the fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained 20 min with APC-conjugated anti-CD24 antibody (1:100 dilution, 138506, Biolegend, San Diego, CA) and FITC-conjugated anti-Epcam antibody (1:500 dilution ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... preincubated for 20 min with anti-mouse CD16/CD32 antibody (TruStain FcX, BioLegend, San Diego, CA, USA, 1:50) in FACS buffer followed by 20 min incubation with fluorescent dye-conjugated ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with PBS and intracellularly stained with PE anti-phospho-p38-MAPK (Thr180/Tyr182; 1:20; BioLegend) ab or PE mouse isotype control IgG1,κ (1:20 ...
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bioRxiv - Cell Biology 2023Quote: ... for 20 min at 4°C in the dark with annexin V-FITC or - pacific blue (1:100, Biolegend), near-IR fixable viability dye (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... FACS gates were set based on CD45 (Alexa Fluor® 594 anti-CD45, 1:20, Biolegend, #368520, clone 2D1), SYTOX Blue and DRAQ5 intensities to isolate live CD45+ and CD45-populations as shown in Supplementary Fig ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed three times with TBS-T (TBS with 0.1% Tween-20) and bound Anxs were detected by immunoblotting using Rat anti-FLAG antibody (1:5000; Biolegend) and ECL (ThermoFisher).
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bioRxiv - Cell Biology 2023Quote: ... cells were labeled with antibodies detecting the following cell-surface markers: CD133-APC (Biolegend, clone 7, dilution 1:20), CD19-PECy7 (Biolegend ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were incubated for 20 min with the following antibodies: Ter119-FITC (BioLegend, Catalog No. 116205, 1/800), Cd45R-FITC (BioLegend ...