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Citations for BioLegend :
5801 - 5850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Secreted levels of IFN-β were measured from BMDM supernatants twenty-four hours following the start of the infection following the Sandwich ELISA protocol published by BioLegend. Anti-IFN-β capture antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Anti-IFN-β capture antibody (BioLegend, SanDiego, CA) was diluted 1:200 in Carbonate Coating Buffer [0.84% w/v NaHCO3 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... while the detection antibody (Biotin anti-mouse IFN-β; BioLegend) island Avidin-HRP (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once with cold HMI-9 and resuspended in 200μl cold HMI-9 with rat anti-mouse IgM-FITC (1:500, Biolegend). After one wash with cold HMI-9 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then stained for 30 min with CD45-BV510 (Biolegend, 103138) and Ly6G-BV421 (Biolegend,127628) ...
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bioRxiv - Microbiology 2023Quote: ... lysed in 1X red blood cell lysis buffer for 3 minutes (ref no. 420301, BioLegend), and passed successively through 70μm and 30μm strainers (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (CD45+ immune cells: 1:50 anti-CD45-VioGreen [Miltenyi Biotec, Germany]; CD31+ endothelial cells: 1:2000 anti-CD31-PE [BioLegend, CA]/1:50 anti-CD45-VioGreen [Miltenyi Biotec ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was incubated in 1% milk-PBS-T containing rabbit anti-LpxC antibody (a generous gift from the Doerrler lab) or mouse anti-RpoA (anti-E.coli RNA polymerase alpha from Biolegend, cat# 663104) diluted 1:10,000 ...
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bioRxiv - Immunology 2023Quote: ... following manufacturer recommendations using a custom LEGENDplex™ (Biolegend) bead-based immunoassay for the following cytokines ...
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bioRxiv - Immunology 2023Quote: ... followed by treatment with RBC lysis buffer (Biolegend, Cat# 420301). BAL cells and single lung cells were first gated by (FSC-A x SSC-A ...
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bioRxiv - Immunology 2023Quote: ... Surfaced staining was conducted using Cell Staining Buffer (Biolegend, Cat# 420201). Intracellular and nuclear staining was carried out with eBioscience™ Intracellular Fixation & Permeabilization Buffer (Cat# 88-8823-88 ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... precision counting beads (BioLegend, San Diego, CA) were added to each sample before flow cytometry analysis ...
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bioRxiv - Immunology 2023Quote: ... Flow cytometry antibodies are from Biolegend [Alexa Fluor® 700 anti-mouse CD45 (30-F11) ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were obtained from Biolegend, Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... All antibodies were purchased from BioLegend (San Diego, CA, USA). The staining took 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Antibodies were obtained from Biolegend, Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Staining of intracellular BCL2 was performed with True-Nuclear™ Transcription Factor Buffer Set (Biolegend) as per manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were first stained with viability stain (Zombie NIR fixable viability kit, BioLegend, San Diego, California, USA) followed by either BV510-αCD45 (clone ...
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bioRxiv - Immunology 2023Quote: ... Brefeldin A (#420601, BioLegend) were used as inhibitors.
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bioRxiv - Immunology 2023Quote: ... anti-IL-6 (clone MP5-20F3, BioLegend), and isotype control (#RTK2071 ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated from the spleen using Mojosort Magnetic Separation System CD8 Naïve T cell Isolation Kit (BioLegend). Anti-CD3ε Ab (clone 145-2111C ...
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bioRxiv - Immunology 2023Quote: ... All T cell populations were first enriched by positive selection with CD4 MojoSort™ beads (Biolegend) before FACS sorting.
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bioRxiv - Immunology 2023Quote: ... and isotype control (#RTK2071, BioLegend) Abs were used ...
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bioRxiv - Immunology 2023Quote: ... anti-IL-23p19 (clone MMp19B2, BioLegend), anti-IL-6 (clone MP5-20F3 ...
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bioRxiv - Immunology 2023Quote: ... Supernatant were collected and measured by ELISA kits according to the manufacturer’s instructions (catalog 438707, 440507, 431414; BioLegend). Absorbance was measured at 450 nm with a microplate reader (Molecular Devices).
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bioRxiv - Immunology 2023Quote: ... and ELISA MAX Deluxe Set mouse TNF-α (BioLegend), respectively ...
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bioRxiv - Immunology 2023Quote: ... brefeldin (Biolegend) and monensin (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... One sample received an irrelevant antibody (a mouse IgG1k against IFNγ from Biolegend) control ...
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bioRxiv - Immunology 2023Quote: ... the culture supernatants were evaluated using the ELISA MAX assay kit (BioLegend), according to the manufacturer’s specifications.
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bioRxiv - Immunology 2023Quote: ... CD138-PE/Cyanine7 (Syndecan-1, clone 281-2, BioLegend), CD27-APC (clone LG.3A10 ...
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bioRxiv - Immunology 2023Quote: ... IgM-APC/Cyanine7 (clone RMM-1, BioLegend), CD86-BV510 (clone GL1 ...
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bioRxiv - Immunology 2023Quote: ... and anti-CD49b antibodies (clone HMa2, BioLegend; Alexa 488), respectively ...
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bioRxiv - Immunology 2023Quote: ... 5 μg of fluorescently conjugated anti-CD31 (clone 390, BioLegend; either Alexa 594) and anti-CD49b antibodies (clone HMa2 ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized using True-Nuclear Transcription Factor Buffer Set (BioLegend, 424401). Permeabilized cells were stained with antibodies (Supplementary Table 3 ...
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bioRxiv - Immunology 2023Quote: ... anti-CD69 (Biolegend, clone: H1.2F3), anti-CD44 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Immunology 2023Quote: ... cells were treated with 81 nM phorbol 12-myristate 13-acetate (PMA) plus 1.34 µM Ionomycin (BioLegend, 423301) for 30 min ...
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bioRxiv - Immunology 2023Quote: ... which included different combinations of IL-12 (Biolegend, #577004), IL-18 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... wells were pre-coated with anti-CD3ε (Biolegend, #145-2C11) prepared in PBS at 1 μg/mL for 2-3 hours at 37°C before being emptied for seeding ...
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bioRxiv - Immunology 2023Quote: ... IL-18 (Biolegend, #767004), IL-15 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... The list of flow cytometry antibodies used in this study were obtained from Biolegend and are presented in the table below ...
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bioRxiv - Immunology 2023Quote: ... IL-1β (Biolegend, #575102), and IL-7 (PreproTech ...
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bioRxiv - Immunology 2023Quote: ... IFN-α (Biolegend, #752804), IFN-β (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (Biolegend, #575404), IL-1β (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... using the MojoSort™ CD8+ T-cell isolation kit and magnets (Biolegend, #480008 and #480019). Isolated T-cells were resuspended in complete RPMI (RPMI 1640 [Gibco ...
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bioRxiv - Immunology 2023Quote: ... blocked with TruStain FcX (anti-mouse CD16/32) antibody (Biolegend, 1:1,000), and stained for extracellular markers at 1:400 dilution ...
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bioRxiv - Immunology 2023Quote: ... IL-33 (Biolegend, #580504), IFN-α (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... wells were pre-coated with 5 μg/mL ICAM-1 (Biolegend, #553006) for 2-3 hours at 37°C before being emptied for seeding ...
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bioRxiv - Immunology 2023Quote: ... IFN-β (Biolegend, #581304), IL-2 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... anti-CD209 (APC conjugated anti-human CD209 Antibody, clone: 9E9A8, BioLegend). For TLR staining experiments ...