citations for BioLegend

Natural malaria infection elicits rare but potent neutralizing antibodies to the blood-stage antigen RH5

Lawrence T. Wang, et al., bioRxiv - Immunology 2023

Quote: ... CD56-BV510 (BioLegend), CD19-ECD (Beckman Coulter) ...

Synovial macrophage diversity and activation of M-CSF signaling in post-traumatic osteoarthritis

Alexander J. Knights, et al., bioRxiv - Immunology 2023

Quote: ... single cell suspensions were washed with FACS buffer (PBS containing 2% fetal calf serum and 1 mM EDTA) and 1 µL of FcX TruStain PLUS (Biolegend) was added to each sample ...

Multimodal single-cell profiling of T cell specificity and reactivity in lung cancer

Florian Bieberich, et al., bioRxiv - Immunology 2023

Quote: ... reactive TnT cells were sorted via anti-CD3 (PE/Cy7, Biolegend), anti-CD69 (APC ...

Multimodal single-cell profiling of T cell specificity and reactivity in lung cancer

Florian Bieberich, et al., bioRxiv - Immunology 2023

Quote: ... The fluorescently-labeled antibody staining consisted of anti-CD8 (BV421, Biolegend), anti-CD4 (APC ...

PD-L1 Restrains the PD-1^hiNrp1^loTGFβ⁺ Treg to block IL6⁺ Neutrophil tumor infiltration to Suppress Inflammation-driven Colorectal Cancer

Dakota B. Poschel, et al., bioRxiv - Immunology 2023

Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...

PD-L1 Restrains the PD-1^hiNrp1^loTGFβ⁺ Treg to block IL6⁺ Neutrophil tumor infiltration to Suppress Inflammation-driven Colorectal Cancer

Dakota B. Poschel, et al., bioRxiv - Immunology 2023

Quote: ... IL-1b, IL-6, IL-10, IL-17A, IL-12p70, GM-CSF, IL-23, IFN-b, MCP-1, IL-27; BioLegend Cat# 740446), Mouse TH Panel v4 (TNF-α ...

Scarring hair follicle destruction is driven by the collapse of EGFR-protected JAK-STAT1-sensitive stem cell immune privilege

Karoline Strobl, et al., bioRxiv - Immunology 2023

Quote: ... cells were stained with Zombie Aqua viability stain (1:200, Biolegend) for 15 min at room temperature ...

Scarring hair follicle destruction is driven by the collapse of EGFR-protected JAK-STAT1-sensitive stem cell immune privilege

Karoline Strobl, et al., bioRxiv - Immunology 2023

Quote: ... The single cell suspension was blocked in FC-block CD16/32 (1:100, Biolegend) and subsequently incubated in Zombie Aqua viability stain (1:200 ...

Enhancer regulatory networks globally connect non-coding breast cancer loci to cancer genes

Yihan Wang, et al., bioRxiv - Genomics 2023

Quote: ... cells were labeled with ten different antibodies using the cell hashing protocol (BioLegend)131 and loaded into 16 lanes ...

Streptococcus agalactiae and Escherichia coli Induce Distinct Effector γδ T Cell Responses During Neonatal Sepsis and Neuroinflammation

Lila T. Witt, et al., bioRxiv - Immunology 2023

Quote: ... IFN-γ ELISAmax (Biolegend), ELISAs were performed according to manufacturer’s instructions ...

Streptococcus agalactiae and Escherichia coli Induce Distinct Effector γδ T Cell Responses During Neonatal Sepsis and Neuroinflammation

Lila T. Witt, et al., bioRxiv - Immunology 2023

Quote: ... 1X protein transport inhibitor (Fisher Healthcare) and 1:1000 PMA/Ionomycin (Biolegend) were added ...

Predicting efficacy of immunotherapy in mice with triple negative breast cancer using a cholesterol PET radiotracer

Nicholas G. Ciavattone, et al., bioRxiv - Immunology 2023

Quote: ... and CD279 (29F.1A12) (Biolegend). Cholesterol probes included Cholesteryl BODIPY 542/563 C11 (Invitrogen ...

Predicting efficacy of immunotherapy in mice with triple negative breast cancer using a cholesterol PET radiotracer

Nicholas G. Ciavattone, et al., bioRxiv - Immunology 2023

Quote: ... specific antibodies included anti-mouse CD3ε (145-2C11) and CD28 (37.51) or anti-human CD3 antibody (OKT3) and anti-human CD28 (CD28.2) (antibodies from Biolegend). For flow cytometry experiments ...

Predicting efficacy of immunotherapy in mice with triple negative breast cancer using a cholesterol PET radiotracer

Nicholas G. Ciavattone, et al., bioRxiv - Immunology 2023

Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...

Multimodal profiling of peripheral blood identifies proliferating circulating effector CD4⁺ T cells as predictors for response to integrin α4β7-blocking therapy in patients with inflammatory bowel disease

Veronika Horn, et al., bioRxiv - Immunology 2023

Quote: ... CD4+ memory T cells were labeled with hashtags after sorting (BioLegend, Suppl. Table 10).

WASP facilitates tumor mechanosensitivity in T lymphocytes

Srishti Mandal, et al., bioRxiv - Immunology 2023

Quote: ... antibodies were purchased either from Biolegend (CD69 ...

Streptococcus agalactiae and Escherichia coli Induce Distinct Effector γδ T Cell Responses During Neonatal Sepsis and Neuroinflammation

Lila T. Witt, et al., bioRxiv - Immunology 2023

Quote: ... Samples were then washed once with FACS buffer and once with 1X perm buffer (Biolegend) and spun down at 1500 rpm for 5 minutes ...

Streptococcus agalactiae and Escherichia coli Induce Distinct Effector γδ T Cell Responses During Neonatal Sepsis and Neuroinflammation

Lila T. Witt, et al., bioRxiv - Immunology 2023

Quote: ... Serum samples were diluted 1:5 in ELISA diluent buffer (Biolegend).

Membrane positioning across antigen-induced synaptic contacts tunes CAR-T cell signaling and effector responses

Fenglei Li, Kaushik Choudhuri, bioRxiv - Immunology 2023

Quote: ... with a difference that the cells were Fc blocked with both human Fc blocker (Human TruStain FcX, Biolegend) and mouse Fc blocker (TruStain FcX™ PLUS (anti-mouse CD16/32 ...

Long-term engraftment of adult hematopoietic progenitors in a novel model of humanized mice

Chun I Yu, et al., bioRxiv - Immunology 2023

Quote: ... CD15-FITC (HI98, Biolegend), CD1c-PerCP-Cy5.5 (L161 ...

Multimodal single-cell profiling of T cell specificity and reactivity in lung cancer

Florian Bieberich, et al., bioRxiv - Immunology 2023

Quote: ... anti-CD3 (PE/Cy7, Biolegend), anti-CD45 (PE ...

Multimodal single-cell profiling of T cell specificity and reactivity in lung cancer

Florian Bieberich, et al., bioRxiv - Immunology 2023

Quote: ... TnT cells were FACS sorted using DAPI and anti-CD3 (PE/Cy7, Biolegend) according to the previously mentioned staining protocol ...

Keratin 6a attenuates Toll-like receptor-triggered proinflammatory response in corneal epithelial cells by suppressing ELKS/IKKε-dependent activation of NF-κB

Jonathan K. Chan, et al., bioRxiv - Immunology 2023

Quote: ... All samples were stained with Zombie Violet (BioLegend) to gate out dead cells ...

A viral-specific CD4⁺ T cell response protects female mice from Coxsackievirus B3 infection

Aryamav Pattnaik, et al., bioRxiv - Immunology 2023

Quote: ... CD49d (clone R1-2, BioLegend, catalog #103618).

A viral-specific CD4⁺ T cell response protects female mice from Coxsackievirus B3 infection

Aryamav Pattnaik, et al., bioRxiv - Immunology 2023

Quote: ... were utilized for the staining: CD4 (clone GK1.5, BioLegend, catalog #100412, #100406), CD8a (clone 53-6.7 ...

Monocytes reprogrammed by 4-PBA potently contribute to the resolution of inflammation and atherosclerosis