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• B cell and antibody targeting of transplant alloantigen epitopes is supported by both self and allo-recognition

  John T. Killian Jr., et al., bioRxiv - Immunology 2023

  Quote: ... including CD21::FITC (Biolegend, clone Bu32), CD19::APC (BD ...
• Apoptotic signaling clears engineered Salmonella in an organ-specific manner

  Taylor J. Abele, et al., bioRxiv - Immunology 2023

  Quote: ... or HA (1:2000, mouse, Biolegend, Cat. MMS-101R). Membranes were incubated for 1 h at room temperature with appropriate secondary antibodies ...
• Repeated Omicron exposures override ancestral SARS-CoV-2 immune imprinting

  Ayijiang Yisimayi, et al., bioRxiv - Immunology 2023

  Quote: ... and TotalSeq™-C0972 Streptavidin (BioLegend, 405273), WT biotinylated RBD protein conjugated with TotalSeq™-C0973 Streptavidin (BioLegend ...
• Repeated Omicron exposures override ancestral SARS-CoV-2 immune imprinting

  Ayijiang Yisimayi, et al., bioRxiv - Immunology 2023

  Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
• Preclinical lentiviral vector-mediated hematopoietic stem and progenitor cell gene therapy corrects Pompe disease-related muscle and neurological manifestations

  John K. Yoon, et al., bioRxiv - Neuroscience 2023

  Quote: ... CD31 clone 390 in PerCP/Cy5.5 (BioLegend). Microglia cells ...
• Enhanced neutralization of SARS-CoV-2 XBB sub-lineages and BA.2.86 by a tetravalent COVID-19 vaccine booster

  Xun Wang, et al., bioRxiv - Immunology 2023

  Quote: ... Monensin (420701, BioLegend; 0.25 μL/well) and 500 μg/mL SARS-CoV-2 spike trimer (2 μL/well ...
• Regulating the proinflammatory response to implanted composite biomaterials comprising polylactide and hydroxyapatite by targeting immunometabolism

  Chima V. Maduka, et al., bioRxiv - Bioengineering 2023

  Quote: ... followed by incubation with TruStain FcX (anti-mouse CD16/32) Antibody (BioLegend, Cat#101319; 1 μg/sample) in 50 μL volume for 10 min ...
• Regulating the proinflammatory response to implanted composite biomaterials comprising polylactide and hydroxyapatite by targeting immunometabolism

  Chima V. Maduka, et al., bioRxiv - Bioengineering 2023

  Quote: ... PerCP MHCII (1:200, Biolegend, cat#107623), PacBlue Ly6G (1:250 ...
• Regulating the proinflammatory response to implanted composite biomaterials comprising polylactide and hydroxyapatite by targeting immunometabolism

  Chima V. Maduka, et al., bioRxiv - Bioengineering 2023

  Quote: ... BV785 F4/80 (1:300, Biolegend, cat#123141), BV421 CD86 (1:200 ...
• Regulating the proinflammatory response to implanted composite biomaterials comprising polylactide and hydroxyapatite by targeting immunometabolism

  Chima V. Maduka, et al., bioRxiv - Bioengineering 2023

  Quote: ... and PE-Dazzle 594 CD11c (1:500, Biolegend, cat#117347). Cells and antibody mixture were incubated for 30 min ...
• Regulating the proinflammatory response to implanted composite biomaterials comprising polylactide and hydroxyapatite by targeting immunometabolism

  Chima V. Maduka, et al., bioRxiv - Bioengineering 2023

  Quote: ... AF700 CD11b (1:300, Biolegend, cat#101222), BV785 F4/80 (1:300 ...
• Regulating the proinflammatory response to implanted composite biomaterials comprising polylactide and hydroxyapatite by targeting immunometabolism

  Chima V. Maduka, et al., bioRxiv - Bioengineering 2023

  Quote: ... APC CD206 (1:200, Biolegend, cat#141707), PerCP MHCII (1:200 ...
• Regulating the proinflammatory response to implanted composite biomaterials comprising polylactide and hydroxyapatite by targeting immunometabolism

  Chima V. Maduka, et al., bioRxiv - Bioengineering 2023

  Quote: ... BV421 CD86 (1:200, Biolegend, cat#105031), APC CD206 (1:200 ...
• Peptide aggregation-induced immunogenic cell death in a breast cancer spheroid model

  Gokhan Gunay, et al., bioRxiv - Bioengineering 2023

  Quote: ... and values were calculated based on the standard curve for IL-6 (BioLegend 431315), IL-1β (BioLegend 432615) ...
• Peptide aggregation-induced immunogenic cell death in a breast cancer spheroid model

  Gokhan Gunay, et al., bioRxiv - Bioengineering 2023

  Quote: ... IL-1β (BioLegend 432615), IL-10 (BioLegend 431414) ...
• Peptide aggregation-induced immunogenic cell death in a breast cancer spheroid model

  Gokhan Gunay, et al., bioRxiv - Bioengineering 2023

  Quote: ... and IL-12p70 (BioLegend 433604). Lipopolysaccharide (LPS ...
• The anti-B7-H3 blocking antibody MJ18 does not recognize B7-H3 in murine tumor models

  Talah Nammor, et al., bioRxiv - Cancer Biology 2023

  Quote: ... F4/80-PE (BioLegend, clone BM8, cat. 123109), B7-H3-AF647 (Abcam ...
• The anti-B7-H3 blocking antibody MJ18 does not recognize B7-H3 in murine tumor models

  Talah Nammor, et al., bioRxiv - Cancer Biology 2023

  Quote: Tumor cell lines and murine splenocytes were stained for flow cytometry assays in FACS buffer (1X PBS, 2 mM EDTA and 2c% FBS) with the following antibody panel: CD3-BV510 (BioLegend, clone 17A2, cat. 100233), NK1.1-PE-Cy7 (BioLegend ...
• Ammonia inhibits antitumor activity of NK cells by decreasing mature perforin

  Joanna Domagala, et al., bioRxiv - Cancer Biology 2023

  Quote: ... clone 3G8 (BioLegend); anti-CD56 ...
• Cancer-associated fibroblasts serve as decoys to suppress NK cell anti-cancer cytotoxicity

  Aviad Ben-Shmuel, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Ultra-LEAF™ anti-mouse CD155 (Biolegend, 942103), anti-mouse Nectin2 (R&D ...
• Cancer-associated fibroblasts serve as decoys to suppress NK cell anti-cancer cytotoxicity

  Aviad Ben-Shmuel, et al., bioRxiv - Cancer Biology 2023

  Quote: ... NKG2D and DNAM-1 surface receptors on NK cells were blocked utilizing 25 μg/ml of purified anti-mouse NKG2D (Biolegend, clone CX5, 130202), purified anti-mouse DNAM-1 (Biolegend ...
• Cancer-associated fibroblasts serve as decoys to suppress NK cell anti-cancer cytotoxicity

  Aviad Ben-Shmuel, et al., bioRxiv - Cancer Biology 2023

  Quote: ... purified anti-mouse DNAM-1 (Biolegend, clone 10E5, 128822), or Mouse IgG1 Isotype Control control (R&D ...
• GRAMD2⁺ alveolar type I cell plasticity facilitates cell state transitions in organoid culture

  Hua Shen, et al., bioRxiv - Cell Biology 2023

  Quote: ... and KRT5 (1:200, #905903, Biolegend). For Edu labelling ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD8a-PE/Cy5 (1:320, Biolegend, clone RPA-T8), anti-CD11b-PE/Cy5 (1:160 ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD11b-PE/Cy5 (1:160, Biolegend, clone ICRF44), anti-CD14-PE/Cy5 (1:160 ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD2-PE/Cy5 (1:160, Biolegend, clone RPA-2.10), anti-CD3-PE/Cy5 (1:320 ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD19-PE/Cy5 (1:160, Biolegend, clone HIB19), anti-CD20-PE/Cy5 (1:160 ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD4-PE/Cy5 (1:160, Biolegend, clone RPA-T4), anti-CD8a-PE/Cy5 (1:320 ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD123-PE (1:40, Biolegend, clone 6H6), anti-CD90-PE/Cy7 (1:20 ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD3-PE/Cy5 (1:320, Biolegend, clone HIT3a), anti-CD4-PE/Cy5 (1:160 ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD90-PE/Cy7 (1:20, Biolegend, clone HI100), anti-CD34-APC (1:160 ...
• Selective advantage of mutant stem cells in clonal hematopoiesis occurs by attenuating the deleterious effects of inflammation and aging

  Niels Asger Jakobsen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD34-APC (1:160, Biolegend, clone 581), anti-CD2-PE/Cy5 (1:160 ...
• Trogocytosis of cancer-associated fibroblasts promotes pancreatic cancer growth and immune suppression via phospholipid scramblase anoctamin 6 (ANO6)

  Charline Ogier, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cell lineage was determined with the lineage markers: CD45 (1:200, #103107, BioLegend), CD11b (1:200 ...
• Analyzing ER stress response in ALS patient derived motor neurons identifies druggable neuroprotective targets

  Michelle E. Watts, et al., bioRxiv - Cell Biology 2023

  Quote: ... and anti-TUJ1 (Biolegend 801202 ...
• A deep lung cell atlas reveals cytokine-mediated lineage switching of a rare cell progenitor of the human airway epithelium

  Avinash Waghray, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-human CD117–FITC or CD117–APC-Cy7 (1:100; BioLegend) for 20 minutes at room temperature ...
• A deep lung cell atlas reveals cytokine-mediated lineage switching of a rare cell progenitor of the human airway epithelium

  Avinash Waghray, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-human EPCAM–APC or EPCAM–PE (1:100; BioLegend), anti-human CD117–FITC or CD117–APC-Cy7 (1:100 ...
• Distinct host preconditioning regimens differentially impact the antitumor potency of adoptively transferred Th17 cells

  Megen C. Wittling, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 20 ng/mL of IL23 (BioLegend) was added to the culture on Days 2 and 3 ...
• Ectopic adipogenesis in response to injury and material implantation in an autoimmune mouse model

  Tran B. Ngo, et al., bioRxiv - Bioengineering 2023

  Quote: ... CD49b-BV421 (Biolegend) was substituted for CD49b- V450 antibody compared to the previous protocol ...
• Physical activity regulates the immune response to breast cancer by a hematopoietic stem cell-autonomous mechanism

  Lyne Khair, et al., bioRxiv - Cancer Biology 2023

  Quote: ... washed with FACS buffer and then re-suspended in PBS containing Zombie Green (Biolegend; cat#423111) following manufacturer’s recommendation.
• Increased multiplexity in optical tissue clearing-based 3D immunofluorescence microscopy of the tumor microenvironment by LED photobleaching

  Jingtian Zheng, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-CD45 (BioLegend, 30-F11), anti-CD3 (BioXcell ...
• Proliferating CLL cells express high levels of CXCR4 and CD5

  Daniel Friedman, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Red blood cells were lysed in blood and spleen samples using RBC-lysis buffer (Biolegend).
• “Non-viral CRISPR/Cas9 Mutagenesis for Streamlined Generation of Mouse Lung Cancer Models.”

  Irene Lara-Saez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-Keratin 5 (PRB-160P, BioLegend, 1:2000); anti-CC10 H-75 (sc-25554 ...
• Aminobisphosphonates reactivate the latent reservoir in people living with HIV-1

  Marta Sanz, et al., bioRxiv - Microbiology 2023

  Quote: ... Cells were washed and stained with a viability dye (Zombie Aqua Fixable viability kit, BioLegend) and fixed with 100µl of pre-warmed Cytofix Fixation Buffer (BD Biosciences ...
• Aminobisphosphonates reactivate the latent reservoir in people living with HIV-1

  Marta Sanz, et al., bioRxiv - Microbiology 2023

  Quote: ... and washed once with 1x Perm/Wash Buffer (PWB, BioLegend). Cells were incubated for 30 min at 37°C with the intracellular antibody cocktail in PWB ...
• Replicative Acinetobacter baumannii strains interfere with phagosomal maturation by modulating the vacuolar pH

  Jesus S. Distel, et al., bioRxiv - Microbiology 2023

  Quote: ... Total cell counts per mouse were calculated using Precision Count Beads (Biolegend, 424902) according to the manufacturer’s instructions.
• Replicative Acinetobacter baumannii strains interfere with phagosomal maturation by modulating the vacuolar pH

  Jesus S. Distel, et al., bioRxiv - Microbiology 2023

  Quote: ... and Ly6G-BV421 (Biolegend,127628), CD11b-Alexa700 (BD Biosciences ...
• Streptococcus pneumoniae drives specific and lasting Natural Killer cell memory

  Tiphaine M.N. Camarasa, et al., bioRxiv - Microbiology 2023

  Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
• Mycobacterium tuberculosis SecA2-dependent activation of host Rig-I/MAVs signaling is not conserved in Mycobacterium marinum

  Lindsay G. Serene, et al., bioRxiv - Microbiology 2023

  Quote: ... island Avidin-HRP (BioLegend) were diluted 1:200 and 1:500 ...
• Streptococcus pneumoniae drives specific and lasting Natural Killer cell memory

  Tiphaine M.N. Camarasa, et al., bioRxiv - Microbiology 2023

  Quote: ... 1X Brefeldin-A (BFA solution 1000X, ref no.420601, BioLegend) was added into each well to block secretion of IFNγ for 4 hours ...
• Streptococcus pneumoniae drives specific and lasting Natural Killer cell memory

  Tiphaine M.N. Camarasa, et al., bioRxiv - Microbiology 2023

  Quote: ... single cell suspensions are incubated with 1X Brefeldin-A for 4 hours at 37°C to block secretion of cytokines (ref no.420601, BioLegend). For all ...