Labshake search
Citations for BioLegend :
5451 - 5500 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: Dead cells were stained using either Zombie UV™ Fixable Viability Kit (Biolegend, 1:1000), Fixable Viability Dye eFluor™ 506 (eBioscience ...
-
bioRxiv - Immunology 2020Quote: ... The remaining cells were then labelled with biotinylated anti-CD45.2 Ab (Clone 104, Biolegend, #109804) at a 1:100 dilution for 30 min ...
-
bioRxiv - Immunology 2019Quote: ... Post isolation the red blood cells were lysed using RBC lysis buffer (Biolegend Catalog # 420301) before being magnetically sorted using the (Pan T cell isolation kit Catalog # 130-095-130 ...
-
bioRxiv - Immunology 2020Quote: ... FcR Blocking Reagent (1 µg IgG/106 cells in 100 µl staining volume; BioLegend, #422301) was applied to decrease the non-specific binding for 10 minutes on ice ...
-
bioRxiv - Immunology 2021Quote: ... cells were stained by using True-Nuclear transcription factor buffer set (Biolegend, San Diego, CA). Briefly ...
-
bioRxiv - Cancer Biology 2021Quote: ... MCM-treatment vs CTRL) were labeled with Cell Hashing antibodies following the manufacturer’s instruction (BioLegend), cells were counted on Countess II automated cell counter (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cell survival analysis was measured by flow cytometry through Annexin-V-Pacific Blue (Biolegend: 640917) and Propidium Iodide staining (ThermoFisher ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were incubated overnight at 4°C with BV421 anti-Foxp3 (Clone MF-14, Biolegend) washed ...
-
bioRxiv - Immunology 2022Quote: ... lung leukocytes (≤107 cells) were blocked with 10 μL of anti-CD32/ CD16 (S17011E, Biolegend) for 10 min at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... cells were incubated with the allophycocyanin cross-linked anti-RANK antibody (BioLegend, San Diego, US). Surface expression was quantified by flow cytometry by using FACS Canto cytometer (BD Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... Cells were washed with 1× PBS and stained with an amine–reactive dye (Biolegend # 423106) for 20 minutes at room temperature to assess cell viability ...
-
bioRxiv - Immunology 2022Quote: ... Cells were stained with a PerCP-Cyanine 5.5-conjugated CD45.1 antibody (BioLegend 110728, RRID: AB_893346). Stained single cell suspensions of all mice were pooled to obtain enough immune cells for sorting ...
-
bioRxiv - Genomics 2019Quote: ... filtered through a 40µm sieve and stained with 7AAD (BioLegend; 50µl/40×106 cells/ml). Small aliquots were taken as single stain controls and stained as above ...
-
bioRxiv - Developmental Biology 2019Quote: ... placental single-cell suspensions were stained with APC-conjugated anti-mouse CD117 (c-kit) (BioLegend), PerCP/Cy5.5-conjugated anti-mouse CD34 (BioLegend) ...
-
bioRxiv - Immunology 2019Quote: ... The cell suspension from ear tissue was stained with anti-CD45-PE (BioLegend, Cat. # 103106). Blood leukocytes were gated as double positive (CD45-BV421+ CD45-PE+ ...
-
bioRxiv - Cell Biology 2020Quote: ... Some cells were stained with biotin-labeled anti-CD11b antibody (BioLegend, San Diego, CA, USA) and streptavidin–fluorescein isothiocyanate conjugate (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2020Quote: ... CD4+ T cells were stimulated with plate bound αCD3 and αCD28 (both 1μg/ml, Biolegend). After 12 hours ...
-
bioRxiv - Immunology 2020Quote: ... Cells were stained for surface expression of CD16 (Clone 3G8 Pacific Blue, Biolegend, Cat # 302032), CD56 (clone 5.1H11 AlexaFluor488 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Non-specific antibody recognition sites on cells were blocked by adding Fc Block (Biolegend, #101301) to the suspension for ten minutes at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were incubated with Zombie UVTM fixable viability kit (BioLegend®, San Diego, CA, USA). Following which ...
-
bioRxiv - Neuroscience 2020Quote: ... Immune cells were separated using a Percoll-gradient and stained with CD45 PerCP (Biolegend 103130), CD11c PE-Cy7 (eBioscience 25-0114)) ...
-
bioRxiv - Microbiology 2021Quote: ... cells were pre-incubated with 10 ug/mL anti-SIGLEC1 antibody (Biolegend, clone 7-239) for 30 min ...
-
bioRxiv - Immunology 2019Quote: ... small fractions of the CD3 negative and positive cells were stained with CD3-FITC (BioLegend) and Zombie Aqua Fixable Viability Dye (BioLegend ...
-
bioRxiv - Immunology 2020Quote: ... the cells were washed once in phosphate-buffered saline and stained with Zombie Yellow (BioLegend) to assess live vs ...
-
bioRxiv - Microbiology 2020Quote: ... cells were assessed for viability using a 30 min RT incubation with Zombie NIR (BioLegend) diluted in PBS ...
-
bioRxiv - Immunology 2021Quote: ... Cells were subsequently blocked with 2 μL of TruStain FcX Fc receptor blocking antibody (BioLegend) for 10 minutes on ice ...
-
bioRxiv - Immunology 2021Quote: ... cells were washed once with 1x PBS and incubated in TruStain FcX-Fc Block (Biolegend) for 15 min at 4°C in the dark ...
-
bioRxiv - Immunology 2021Quote: ... Stimulated Jurkat cells and PBMCs were stained with TCR α/β–APC antibody (Biolegend, #306717) CD69-PE (Biolegend ...
-
bioRxiv - Cancer Biology 2020Quote: ... Dead cells were excluded using 7-aminoactinomycin D (7-AAD, eBioscience) and zombie violet (BioLegend). FACS analysis was conducted using a MACSQuant analyzer (Miltenyi Biotec) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were then stained with Brilliant Violent 421-conjugated ms α-EGFR (Biolegend; Table 2). Samples were gated for intact GFP+ cells and geometric mean of fluorescence intensity (geoMFI ...
-
bioRxiv - Microbiology 2020Quote: ... Lung cells then were stained as described above except that CD4-BV421 (BioLegend clone GK1.5) replaced the CD4-PE-Cy5 ...
-
bioRxiv - Cell Biology 2021Quote: ... The fixed cells were then stained with chicken anti-vimentin (1:200, 919101, Biolegend, USA) and rabbit anti-lamin A (to determine the location of the nucleus ...
-
bioRxiv - Developmental Biology 2020Quote: ... Then cells were stained with PE coupled antibody to mouse NCR1 (29A1.4, BioLegend, CA, USA) and APC coupled antibody to mouse CD45 (30-F11 ...
-
bioRxiv - Genomics 2021Quote: ... CD19-APC (clone HIB19) and CD20-V450 (clone L27) antibody in cell staining buffer (BioLegend) for 10 min at 4C ...
-
bioRxiv - Cell Biology 2020Quote: ... Annexin V staining was performed on surface-stained cells according to manufacturer’s suggestions (Biolegend 640906). BMECs that were DAPI-Annexin V+ were defined as apoptotic.
-
bioRxiv - Immunology 2021Quote: ... 2×106 cells were incubated for 5 hours with Brefeldin A (BioLegend, San Diego, California) and Monensin (BioLegend ...
-
bioRxiv - Neuroscience 2021Quote: ... and NIR for exclusion of dead cells (1:103, #425301, BioLegend, San Diego, CA, USA). Using flow cytometry (BD Bioscience) ...
-
bioRxiv - Cancer Biology 2021Quote: Circulating immune cells were labeled using intravenously injected APC-conjugated CD45 antibody (30-F11, Biolegend) 3 minutes prior to sacrifice as previously described (39,93).
-
bioRxiv - Cell Biology 2021Quote: ... Cells were washed with differentiation medium and resuspended in differentiation medium + DAPI (1.35μg/ml, Biolegend) for flow cytometry analysis (BD LSRIIA ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were washed with differentiation media and resuspended in differentiation media + DAPI (1.35μg/ml, Biolegend) for FACS (BD FACSAria) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were collected and resuspended in 1mL PBS with Zombie Violet™ 1000x (BioLegend®) to exclude dead cells ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were washed in PBS without serum and incubated with Zombie Aqua viability dye (BioLegend) according to manufacturer’s instructions and subsequently washed with FACS buffer (PBS supplemented with 2.5% fetal bovine serum ...
-
bioRxiv - Immunology 2021Quote: ... Cells were activated in a 24-well plate coated with anti-CD3 (clon: 500A2, Biolegend) and soluble anti-CD28 (clon ...
-
bioRxiv - Immunology 2021Quote: ... tetramer specific CD8+ T cells proportion and apoptosis marker Annexin V-APC (BioLegend Cat#640919) on T2A2 cells were evaluated after 16 hours and 7 days ...
-
bioRxiv - Immunology 2021Quote: ... Collected cells were rinsed twice with PBS and stained with Zombie-UV viability dye (BioLegend). Cells were then stained with fluorophore-conjugated antibodies diluted in Brilliant Stain Buffer (BD) ...
-
bioRxiv - Immunology 2020Quote: ... Six hours following co-incubation NR1 cells were harvested and stained for intracellular IFNγ (BioLegend) using an intracellular cytokine staining kit (BioLegend ...
-
bioRxiv - Immunology 2020Quote: ... Cells were washed and surface staining performed using mouse–specific fluorescent antibodies purchased from Biolegend. Different myeloid cell populations were analyzed in the live immune cells (Zombie Aqua-CD45+ ...
-
bioRxiv - Immunology 2021Quote: ... The cells were washed with PBS and stained with Zombie UV fixable viability dye (BioLegend). The cells were washed with PBS containing 5% FCS ...
-
bioRxiv - Immunology 2020Quote: ... cells were pelleted at 500xg and resuspended in Intracellular Staining Perm Wash Buffer (Biolegend, 421002). Three total washes in Intracellular Staining Perm Wash Buffer were performed ...
-
bioRxiv - Immunology 2020Quote: ... Cells were washed twice and stained with Zombie Aqua Live/Dead (Biolegend, San Diego, CA) stain kit for 20 min on ice ...