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Citations for BioLegend :
5601 - 5650 of 6161 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... VDR overexpressing SKOV-3 and ECC-1 cells were processed and stained with PDL1-BV605 antibody (Biolegend Inc, Catalog number:124321). VDR expressing SKOV-3 and ECC-1 cells ...
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bioRxiv - Bioengineering 2022Quote: ... OT-I T cells were isolated using a negative selection cocktail containing the following biotinylated mouse antibodies (BioLegend; San Diego, CA): TCR γ/δ (clone GL3) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with rat polyclonal T antigen antibody (1:500) followed by an anti-rat secondary (Biolegend, 1:200). For measuring sialic acid binding dependence ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue homogenates were centrifuged to remove tissue debris before being incubated with Anti-HA.11 Epitope Tag Antibody (Biolegend, Cat# 901515) for 4 hours in a microcentrifuge tube on a microtube rotator kept at 4°C ...
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bioRxiv - Immunology 2022Quote: ... the plate was washed five times with PBST and incubated with anti-mouse secondary antibody with minimal cross reactivity with human IgG (Biolegend, 405306). The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend ...
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in 100 μL of the FCM buffer with 5μL of the secondary Antibody (PE-anti human IgG Fc Recombinant, Biolegend, Catalogue 366903) and incubated for 30 minutes at 4°C. ...
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bioRxiv - Molecular Biology 2022Quote: ... Whole-lung suspension was then repelleted and resuspended in SB + 1:50 TruStain FcX™ antibody (anti-mouse CD16/32, BioLegend) for a 10-minute blocking period at 37°C ...
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bioRxiv - Microbiology 2022Quote: Lung tissue leukocyte samples were subjected to viability staining and blocking of Fc-receptors using LIVE/DEAD™ Fixable Blue Dead cell stain kit (life technologies) and an anti-CD16/32 antibody (clone 93, BioLegend). Cells were then washed and incubated with a staining mix containing antibodies against the following murine antigens ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for removal of mouse stromal cells included: anti-mouse H-2Kd-biotin (BioLegend, 116604, clone SF1.1, dilution 1:20) and anti-mouse Cd45-PE/Cyanine5 (BioLegend 103110 ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Cells from each strain were then labeled with TotalSeq™-A0301 anti-mouse Hashtag Antibody against CD45 and MHC-I (BioLegend, Clone M1/42 and 30-F11 ...
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bioRxiv - Bioengineering 2022Quote: ... Single cell suspensions of pericytes were stained for 30 minutes at RT using fluorescence-labelled antibodies for pericyte surface markers (488-NG2 from Invitrogen, APC PDGFR-β and APC CD105 from Biolegend) and DAPI for cell viability ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then harvested and stained with phycoerythrin (PE) conjugated antibodies to CD49d or CXCR4 (Biolegend, San Diego, CA, USA). The expression of both proteins on viable cells was assessed by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... 5ug/mL) and anti-CD28 (clone 37.51; 2ug/mL) in the presence of IFN-γ neutralizing antibody (XMG1.2, BioLegend, 10ug/mL) and rmIL-4 (R&D ...
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bioRxiv - Immunology 2022Quote: ... 5ug/mL) and anti-CD28 (clone 37.51; 2ug/mL) in the presence of IL-4 neutralizing antibody (11B11, BioLegend, 5ug/mL) for 18-20 hours before the addition of cytokines as follows ...
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bioRxiv - Immunology 2022Quote: ... for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11), CD11b (BioLegend clone M1/70 ...
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bioRxiv - Cancer Biology 2022Quote: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance ...
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bioRxiv - Neuroscience 2020Quote: ... The following antibodies were then added to cell suspensions at 1:200 concentrations: CD11b-PE (BioLegend Cat#: 101208; Clone: M1/70), CD45-APC/Cy7 (BioLegend Cat# ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed three times with PBS-T (PBS with 0.1% Tween-20) and 50μl of mouse IgG-specific secondary antibody (BioLegend #405306, 1:10,000) diluted in dilution solution added to each well ...
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bioRxiv - Immunology 2020Quote: ... were added to the tumor cells and incubated for 2 hours with CD20-TCB (200 ng/ml) and +/- αLFA1 antibody (Clone: TS 1/18, 10 μg/ml, BioLegend, 302116). Cells were then gently washed ...
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bioRxiv - Microbiology 2021Quote: ... were injected intravenously into the tail vein with 10-15 μg of Alexa Fluor™ 647-coupled anti-CD31 antibody (clone 390, Biolegend) to label blood vessels ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies were added at the concentration of 1:50 and incubated on ice for 45 minutes: APC/Cy7 Cd45 (Biolegend #103116), APC Pecam1 (Biolegend #102410) ...
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bioRxiv - Developmental Biology 2021Quote: ... Surface staining was done by adding the flow cytometry antibody cocktail outlined in Extended Data Table 6 containing F4/80 (BioLegend, 123120), CD11b (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were live-stained for two hours using a conjugated antibody targeted against an OVA257-264 peptide antigen (BioLegend, 141605). The cells were then fixed with 4% paraformaldehyde and imaged using the PerkinElmer Operetta CLS High-Content Analysis System ...
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bioRxiv - Cell Biology 2021Quote: ... The following antibodies were used for cell sorting of lin-CD34+GFP+BFP+ HSPCs after CRISPR editing: CD34 APC (Biolegend 343510), CD11b APC-Cy7 (Biolegend 301342) ...
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bioRxiv - Systems Biology 2022Quote: ... membranes were blocked with 5% non-fat milk at room temperature (RT) for 1 h and probed with primary RpoC (β prime subunit of RNAP) antibodies at 1:2000 dilutions (Biolegend) at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... prM-DENV2-treated PBMCs were treated (1h, 37 °C) in the presence or absence of anti-TNF-α antibody (4 µg/mL, BioLegend). The TNF-α depleted supernatants were used to stimulate HUVEC for 6 hours and the expression of activation markers were analyzed by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... the same panel was used with the replacement of CD4 BV750 antibody with CD8 APC (clone 53-6.7) (Biolegend Cat. 100712) and probing for IFNγ only ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were then treated with 25 μl of APC-antiHA antibody with 1:75 dilution (~0.07 μg/well, BioLegend catalog # 901523) for 1 hour on ice in the dark ...
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bioRxiv - Immunology 2022Quote: ... Every 106 B cells in 100 μl solution were stained with 3 μl FITC antihuman CD20 antibody (BioLegend, 302304, clone: 2H7), 3.5 μl Brilliant Violet 421 antihuman CD27 antibody (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... after 3 days of pre-culturing or by FACS sorting using the following antibodies: Brilliant Violet 510™ hCD45 (cat#368526 from Biolegend), CD33-FITC (cat#IM1135U from Beckman Coulter) ...
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bioRxiv - Immunology 2020Quote: Expanded CD8+ T cells from normal donors and ARPC1B-deficient patients were stained with fluorochrome-coupled antibodies recognizing the extracellular markers CD8 (BioLegend, 344718) and LFA-1 (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Immunology 2020Quote: ... for 10 minutes at 4°C and then incubated for 30 min at room temperature with primary antibodies including CD45-BV785 (BioLegend, 103149) CD11b-PE (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Neuroscience 2021Quote: ... All slides were processed at the same time using the purified mouse monoclonal anti-beta-amyloid 1-16 antibody (6E10) (Biolegend, 803001) at 1/500 for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with the following primary mouse antibodies (1:100 dilution) for 30 minutes at 4°C: CD45-BV750 (clone 30-F11, Biolegend, 103157), CD31-PE-Cy5.5 (clone MEC 13.3 ...
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bioRxiv - Immunology 2020Quote: ... TnT cells and Jurkat-derived cell lines were stimulated overnight with either 10 μg ml−1 plate-bound anti-human CD3e antibody (clone OKT3, BioLegend, #317326) or 1X eBioscience Cell Stimulation Cocktail (81 nM PMA ...
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bioRxiv - Immunology 2021Quote: Four different TotalSeq™-C antibodies were used for Hash Tag Oligonucleotide (HTO) labelling before cell sorting (Biolegend, M1/42M30-F11) following the manufacturer’s standard protocol ...
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bioRxiv - Immunology 2021Quote: ... Approximately 2 million cells per experimental condition were stained with 1 μg of TotalSeq™ B0301-B0308 anti-mouse Hashtag antibodies (BioLegend) according to the manufacturer’s instructions and pooled together at equal cell numbers ...
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bioRxiv - Cell Biology 2020Quote: ... The antibodies used for flow cytometry were as follows: PE-conjugated mouse anti-human CD29 (Biolegend, San Diego, CA, USA; 303004), PE-conjugated mouse anti-human CD73 (BD Biosciences ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membrane was next added to 5% nonfat milk in TBST containing horseradish peroxidase-conjugate goat anti-mouse IgG1 secondary antibodies (Biolegend 405306) diluted at 1:5000 and incubated on a horizontal rocker for 60 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to 2 ml tubes for incubation with the following antibodies diluted in media containing FBS: CD45-PE/Cy7 (BioLegend, 103114), ICAM2-A647 (Life tech ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were isolated and stimulated for 48 h with 3 μg/ml plate-bound anti-mouse CD3 antibody (100340, BioLegend, USA) and 2 μg/ml soluble anti-mouse CD28 antibody (102116 ...
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bioRxiv - Genetics 2021Quote: ... A antibody panel was used to identify and sort the CD3+CD8+ T cell population: Human TrueStain FcX (Biolegend; Cat# 422302), PE anti-human CD45 (Biolegend ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Immunology 2020Quote: Fc block was performed using TruStain FcX™ CD16/32 antibody (anti-mouse) to avoid non-specific binding (101320 Biolegend, UK) according to the supplier protocols The fluorochrome-conjugated antibodies used in the panel were obtained from Biolegend ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Cells were resupsended and incubated for 30 min on ice with primary antibodies including Sca-1-APC (Biolegend [Clone D7; 108112]), CD45-AF488 (Biolegend [Clone 30-F11 ...
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bioRxiv - Immunology 2021Quote: ... Monocyte isolation was determined with the following flow cytometry antibody panel after 10 min incubation with anti-human CD32 (StemCell – 18520): APC anti-human CD14 (Biolegend – 301808), PE-Cy7 anti-human CD3 (Biolegend – 344186) ...