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Citations for BioLegend :
5501 - 5550 of 6161 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Membranes were washed in TBS-T and incubated overnight at 4 ◦C with monoclonal pNF-κB p65 (Ser 529)-specific antibody (1:500 dilution, clone 817403, Biolegend), monoclonal NF-κB p65-specific antibody (1:2500 dilution ...
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bioRxiv - Immunology 2024Quote: ... a combined antibody solution was prepared in FACS buffer with fluorophore-conjugated antibodies: anti-mouse Alexa fluor 700 CD45 (Cat. No. 103128, BioLegend), anti-mouse PerCP/Cyanine 5.5 CD11b (Cat ...
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bioRxiv - Immunology 2024Quote: Immune cells isolated above were stained with the following antibodies (Live/Dead Fixable NIR, Invitrogen, #L34975, 1:500; CD45, Pacific Blue, Biolegend, #103126 ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells from the mouse ear were sorted with a FACSAria sorter from samples treated with BHI-tween or HSV-1 and labeled with TotalSeqC hashtags antibodies (Biolegend). All samples were pooled together ...
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bioRxiv - Immunology 2024Quote: ... Fluorescently labeled anti-mouse antibodies against CD45 (104) and H-2Kb/H-2Db (28-8-6) were obtained from BioLegend.
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bioRxiv - Immunology 2024Quote: ... samples were stained with the antibody cocktail for ImageStream analysis (UCHT1-PE, Biolegend; CD19- AF594, Biolegend; CD14-BV421, Biolegend; CD8-AF700, Biolegend; CD4-APC/Fire750 ...
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Kir6.1, a component of an ATP-sensitive potassium channel, regulates natural killer cell developmentbioRxiv - Immunology 2024Quote: ... either whole spleen or isolated NK cells were incubated in PEB buffer (PBS+0.5% BSA+2mM EDTA) with TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend) on ice for 5 minutes ...
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bioRxiv - Immunology 2024Quote: ... a combined antibody solution was prepared in FACS buffer with fluorophore-conjugated antibodies: anti-mouse Alexa fluor 700 CD45 (Cat. No. 103128, BioLegend), anti-mouse PerCP/Cyanine 5.5 CD11b (Cat ...
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bioRxiv - Immunology 2024Quote: ... The cell suspension was then either resuspended in the appropriate media for in vitro culture (discussed below) or incubated with 5µl/1ml/lung anti-mouse CD16/32 antibody (BioLegend, USA) for 30min at 4°C before further processing.
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bioRxiv - Immunology 2024Quote: ... and stained in media for 20 min at RT with Fixable Viability Dye 780 (eBioscience) and surface antibodies for CD19 (6D5, Biolegend), CD8α (53-6.7 ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed again and stained a second time with a Pacific-Blue-conjugated anti-CD3 antibody (clone UCHT1, Biolegend) and a LIVE/DEAD-UV dye (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... The antibodies in the MDSC mastermix were used at individually titrated dilutions and included: CD33 clone P67.6 (Pe-Cy7, Biolegend #366617), CD14 clone MθP9 (APC-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... Pelleted cells were suspended in FACS buffer and stained for 30 min at 4 °C with the desired antibody cocktails (BioLegend) of APC-Fire 750-anti CD11b (Cat ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged and then resuspended in FACS buffer containing an antibody cocktail consistent of anti-CD11b AF488 (101217; Biolegend), anti-CD45 APC/Cy7 (157618 ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the MDSC panel consisted of the following primary antibodies diluted in FACS buffer: anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Immunology 2024Quote: Surface staining of lung single cell suspensions was performed in PBS with 2% Newborn Calf Serum on ice for 30 minutes with the following antibodies: TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Physiology 2024Quote: ... The cell suspensions were treated with the fluorescent dye-conjugated antibodies and were labeled with Zombie Aqua Viability Dye (BioLegend). Then the cell suspensions were fixed with 4% paraformaldehyde ...
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bioRxiv - Pathology 2024Quote: PFA-fixed tissue sections were blocked with normal donkey serum (10% vol/vol) and incubated at 4 °C overnight with primary antibodies anti-F4/80 (123102, BioLegend), anti-Col1a1 (NB600-408 ...
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bioRxiv - Molecular Biology 2024Quote: ... TNFSF14 neutralization was achieved with a mouse anti-mouse LIGHT blocking antibody (clone 3D11, isotype control mouse IgG2b, clone 27-35, BioLegend), kindly provided by Prof ...
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bioRxiv - Molecular Biology 2024Quote: ... Primary antibodies were incubated overnight at 4°C in a humidified chamber: mouse anti-acetylated tubulin (1:400, BioLegend, 802001) and rabbit anti-GFP (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with cold methanol for 3 min at room temperature and incubated overnight at 4 °C with an anti-HA antibody (diluted 1:2000) (Biolegend,). Cells were then rinsed three times with PBS and incubated for 1 h at room temperature with an Alexa647-conjugated goat anti-mouse antibody (diluted 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... the cells were harvested and labelled on the surface with the following monoclonal antibodies (Abs) from Biolegend (San Diego, USA): APC-conjugated anti-CD3 Abs (clone Mouse IgGak OKT3) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were incubated with biotinylated anti-mouse antibodies (CD31, 1:150, BioLegend; CD45, 1:150, BioLegend; and Sca-1, 1:150, BioLegend) at 4°C for 40 min ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells isolated from WT mice spleen and LN were treated with or without 10 µg/ml anti-β2 integrin non-function blocking antibody (BioLegend) for 1 hour and added onto 96 well plate coated with anti-CD3/CD28 along with either 2.5µg/ml of rLum or rBgn or rDcn or 1µg/ml of ICAM-1 for 30 minutes to check phospho-Akt-1 expression or 6 hours to check activation CD69 expression or 72 hours to check proliferation (CD4+ T cells were pre-labeled with CFSE for proliferation assay) ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were then negatively selected from the mix by incubating on ice for 15 min with biotin conjugated antibodies: CD19 (115504, BioLegend) at 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Fcγ receptors were blocked prior to surface antigen staining by incubating the cells in flow cytometry staining buffer containing Human TruStain FcX antibody (BioLegend) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... Whole blood was immediately stained for flow cytometric analysis with combinations of the following human specific antibodies: CD4 (BV421, RPA-T4,Biolegend), CD4 (APC-Cy7 ...
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bioRxiv - Immunology 2024Quote: ... cells were stained for flow cytometry analysis with the following human specific antibodies: HLA-A,B,C(PE-Cyanine7, W6/32, Biolegend), CD4 (BV421 ...
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bioRxiv - Immunology 2024Quote: ... Isolation purity was verified by flow cytometry using antibodies against neutrophil surface markers anti-human CD11b (Alexa Fluor 594) (Biolegend) and anti-human CD15 (SSEA-1)(Alexa Fluor 488 ...
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bioRxiv - Immunology 2024Quote: ... Then cells were incubated in the dark for 20 minutes with 50µL of the antibody mixture: 1:50 each of anti-CD3-AF488 (BioLegend 100210), anti-CD4-BV510 (BioLegend 100559) ...
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bioRxiv - Bioengineering 2024Quote: ... and vendor information of antibodies used for the flow panel in this study was as follows: (i) Live/Dead (BV510, 1:100, 423111; BioLegend), (ii ...
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bioRxiv - Immunology 2024Quote: ... Surface CAR expression was stained for with an FMC63-biotin antibody (CytoArt) followed by a secondary staining with streptavidin-PE (BioLegend) in 1xPBS for 20 min at 4 °C protected from light ...
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bioRxiv - Immunology 2024Quote: ... they were randomly divided into two groups and one group was treated with IL-27-neutralizing antibody (Anti-mouse IL-27p28, Clone: MM27-7B1, Ultra LEAF purified; Biolegend) and the other with a non-relevant control antibody of the same isotype (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... After extensive washes by TBST, immunoblots were incubated with horseradish peroxidase-conjugated secondary antibodies (GE, IL) or peroxidase-conjugated avidin (BioLegend), and signals were detected using chemiluminescence.
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bioRxiv - Immunology 2024Quote: ... and customized TotalSeqTM-anti-mouse Hashtag Antibodies devoid of anti-CD45 (#155831, #155833, #155835, #155839, #155841, #155843) were purchased from Biolegend. Anti-Shp2 (clone 79 ...
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bioRxiv - Immunology 2024Quote: ... and fluorochrome-conjugated anti-CD86 and anti-CD32 antibodies in FACS buffer (1%BSA and 2 mM EDTA in PBS) supplemented with True Stain Monocyte Blocker solution (Biolegend) to prevent aspecific binding of fluorochrome-conjugated antibodies to myeloid cells ...
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bioRxiv - Immunology 2024Quote: ... incubated for 4 minutes at room temperature, centrifuged (3 min, 500g, 4C) and re-suspended with FACS buffer containing CD16/CD32 blocking antibodies (BioLegend) for 15 minutes on ice ...
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bioRxiv - Immunology 2024Quote: Cells were collected and washed in FACS buffer (phosphate-buffered saline supplemented with 2% fetal bovine serum) and subsequently stained with the following antibodies purchased from BioLegend ...
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bioRxiv - Genomics 2024Quote: ... Lineage depletion was performed using magnetic columns (Miltenyi) with a custom biotinylated lineage antibody cocktail (CD8a, CD4, CD11b, Gr1, Ter119, B220; BioLegend) with streptavidin magnetic beads (Miltenyi ...
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bioRxiv - Immunology 2024Quote: ... Each cell sample in the same group was hash-tagged with a unique TotalSeq anti-mouse MHC-I antibody (Biolegend) on ice for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were washed with PBS containing 2 mM EDTA and stained with antibodies against CD59 (1:100, anti-human CD59 PE, Biolegend) and C3c (1:25 ...
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bioRxiv - Genomics 2024Quote: ... Primary antibody staining was carried out in the dark at 4 C for 30min with the following antibody dilutions in FACS buffer: 1:200 ICAM-1 (353102, Biolegend), 1:500 VCAM-1 (14-1069-82 ...
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bioRxiv - Biochemistry 2024Quote: ... for 15 min followed by the indicated antibodies cocktail for 60 min in the dark on ice: CD45 (FITC, clone S18009F, Biolegend), CD11b (BVC421 ...
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bioRxiv - Neuroscience 2024Quote: ... The remaining 80% cleared supernatant was transferred to a clean RNase-free tube and incubated with mouse anti-HA antibody (1:250; clone 16B12, Biolegend) on a rotating mixer for 4 h at 4°C while 300 uL protein G magnetic beads (Dynabeads ...
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bioRxiv - Immunology 2024Quote: ... ECS was performed during 15 min at room temperature with mix of anti-CD27 (O323)-FITC (or anti-IgG1 (MOPC-21)-FITC isotype control) and CD14 (QA18A22)-APC/Cy7 antibodies (Biolegend). Cells were fixed with fixation buffer (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cells from individual skin tissue samples were simultaneously stained with corresponding mouse TotalSeq-B hashtag index antibodies (B0251-B0260; Biolegend), FACS antibody cocktail (CD45 ...
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bioRxiv - Bioengineering 2024Quote: ... Surface staining was carried out using a fluorochrome-labeled PE-conjugated anti-human CD11a (HI111) monoclonal antibody obtained from BioLegend.
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bioRxiv - Bioengineering 2024Quote: ... a staining solution was prepared by adding 5 µL of each fluorescent-conjugated antibody (anti-CD20-FITC, anti-CD45-APC, anti-CD56-APC-AF750, Zombie Violet; BioLegend) to FACS buffer ...
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bioRxiv - Bioengineering 2024Quote: ... for 5 mins on ice and stained with fluorochrome conjugated antibodies: CD19 (PerCP-Cy5.5, clone: 1D3, BioLegend, cat. Number 152406), CD95 (PE-Cy7 ...
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bioRxiv - Cancer Biology 2024Quote: ... ∼1×10.6 cells were stained with antibody cocktail at 4 °C for 20 min: Pacific Blue™ (PB) anti-mouse CD45.2 (Biolegend), APC anti-mouse B220 (Biolegend) ...