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Citations for BioLegend :
5701 - 5750 of 6161 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was incubated in 1% milk-PBS-T containing rabbit anti-LpxC antibody (a generous gift from the Doerrler lab) or mouse anti-RpoA (anti-E.coli RNA polymerase alpha from Biolegend, cat# 663104) diluted 1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... and APC/Fire 750 conjugated anti E-Cadherin (dilution: 1:100; clone DECMA-1. All antibodies purchase from BioLegend (CA, USA). Samples were washed in 1mL 2% FBS and PBS and centrifuged at 2000rpm for 5mins ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Cancer Biology 2023Quote: ... murine thymocytes were first stained using CD4 and CD8 antibodies and then fixed and permeabilized using Fixation Buffer (Biolegend cat 420801) and Phosflow Perm Buffer III (cat 558050 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Microbiology 2023Quote: Lung single-cell suspensions were incubated with TruStain FcX™ (anti-mouse CD16/32) antibody (Cat: 101320; BioLegend, San Diego, CA) in eBioscience™ Flow Cytometry Staining Buffer (Cat ...
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bioRxiv - Molecular Biology 2023Quote: WT and dcp2Δ strains were cultured in triplicate in YPD medium to A600 of 0.6–0.8 and treated with formaldehyde as previously described (Qiu et al. 2016). ChIP-Seq was conducted as described (Qiu et al. 2016) using monoclonal antibody against Rpb1 (8WG16, Biolegend, 664906). DNA libraries for Illumina paired-end sequencing were prepared using the DNA Library Prep Kit for Illumina from New England Biolabs (E7370L) ...
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bioRxiv - Neuroscience 2023Quote: ... libraries were prepared according to the Chromium Single Cell 3ʹ with Feature Barcoding technology for Cell Surface Protein Reagent Kit v3 protocol (10X Genomics) with modifications for incorporation of TotalSeq™-A HTO antibodies (clones M1/42; 30-F11, BioLegend). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... the same number of cells (106 cells/100 µL) were stained with specific antibodies of CD31 (EC marker, eBiosciences 14-0311082) and CD45 (immune cell marker, BioLegend 103108) for 30 min on ice ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with Alexa Fluor® 488-labeled anti-human CD69 antibody or an isotype control (1 µg/ml, BioLegend) for 45 min ...
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bioRxiv - Microbiology 2023Quote: ... The following antibodies were used for flow cytometry analysis: FITC-conjugated anti-CD19 (6D5) (BioLegend, San Diego, CA, USA, cat# 115505), APC-conjugated anti-CD11b (M1/70 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged at 1000 rpm and resuspended in flow buffer containing the primary antibodies: rat GFP (Biolegend, 338001, 1:200), guinea pig MAP2 (Synaptic Systems ...
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bioRxiv - Biophysics 2023Quote: ... biotinylated anti-mouse CD3e (Cat# 100304) and FITC anti-mouse TCRb antibody (Cat# 109206) were purchased from BioLegend (San Diego, CA). Azide-PEG4-NHS ester (Cat# AZ103-100 ...
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bioRxiv - Immunology 2023Quote: ... CAR-transduced (thus EGFRt+) T cells were detected by staining with anti-EGFR antibody (clone AY13, Biolegend, San Diego, CA, USA) and scFv-Linker (custom made ...
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bioRxiv - Immunology 2023Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Immunology 2023Quote: ... intracellular FACS analysis use only) allophycocyanin (APC)/Cyanine7 or Brilliant Violet 421™ anti-human CD45 antibody (1:200, Biolegend, HI30), APC anti-human CD31 antibody (1:200 ...
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bioRxiv - Cell Biology 2023Quote: Blood was collected from mice via the retro-orbital sinus and red blood cells were lysed prior to staining with the following fluorochrome-conjugated antibodies: BV650 CD45.1 (BioLegend clone A20), AlexaFluor700 CD45.2 (BioLegend clone 104) ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times before adding 50 µL per well of Biotin anti-human IFN-γ Antibody (clone 4S.B3; #502504, BioLegend, USA) diluted 1:400 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Bioengineering 2023Quote: 50 μm streptavidin-coated nanovials were reconstituted at a five-time dilution in Washing Buffer containing 270 nM (40 μg/mL) of biotinylated anti-CD63 antibody (Biolegend, 353018). Nanovials were incubated with antibodies for 30 minutes at room temperature on a rotator and washed three times as described above ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were using 1:500 rabbit anti Abeta 1:500 (IBL, CAT # 18584) and 1:500 mouse anti SMI312 (Biolegend 837904).
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary anti-HA antibodies diluted in PBS-tween 0.2% blocking medium (mouse anti-HA, 1:5000, Biolegend cat. # 901513) over night at 4°C under agitation ...
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bioRxiv - Immunology 2023Quote: Cryopreserved PBMCs from blood collected 1 and 2 weeks after the H2-F boost were stained with anti-human monoclonal antibodies CD3 BV510 (OKT3, 1:400 dilution, BioLegend, RRID:AB_2561376), CD56 BV510 (HCD56 ...
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bioRxiv - Immunology 2023Quote: ... The levels of peptide-loaded MHC-I on the beads were tested using the PE-conjugated W6/32 antibody (Biolegend, 311406). Binding levels were measured in Luminex 100 Liquid Analyzer System and the results analyzed in GraphPad Prism v9.
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bioRxiv - Immunology 2023Quote: ... Reaction was stopped by centrifugation at 4°C and externalized LAMP1 (CD107a) was stained using FITC-coupled anti-LAMP1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... Alexa Fluor 488 or PE–conjugated rat anti-mouse CD31 [platelet endothelial cell adhesion molecule 1 (PECAM1)] antibody (1:200; BioLegend, MEC13.3), Alexa Fluor 647 or FITC–conjugated rat anti-mouse CD326 (Ep-CAM ...
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bioRxiv - Bioengineering 2023Quote: ... Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The digested tissue was resuspended in 100 μL of 4% FBS in PBS containing an FITC antibody to CD11b (1:100; BioLegend, 101206) and PE antibody to CD45(1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections of PDX tumours and their equivalent primary tumour region were subjected to hematoxylin and eosin (H&E) staining or immunohistochemistry using the following antibodies: anti-human CD45 (Clone: HI30; 1:200; BioLegend, #304002), anti-keratin (Clone ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... cells were resuspended in FACS buffer and incubated with Alexa Fluor® 488 anti-CD31 ([390] 102414) and APC/Cyanine7 anti-CD45 ([30-F11] 103116) antibodies (1/100, BioLegend) and mouse TruStain FcXTM (1/200 ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for CTC detection of human CTCs in animal models contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106) and mouse anti-human HLA conjugated to AlexaFluor 647 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Splenocytes were washed once and stained with the following antibodies for 30 minutes: anti-CD4 Alexa Fluor 488 (BioLegend Cat: 100529), anti-CD8a Brilliant Violet 510 (BioLegend Cat ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by centrifugation at 4°C followed by staining of cell surface LAMP-1 (CD107a) using FITC-coupled anti-LAMP-1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used at a concentration of 1 µg/mL except where otherwise indicated: APC-Cy7-CD117 (clone 2B8; Biolegend #105826), PE-FceRI (clone MAR-1 ...
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bioRxiv - Neuroscience 2023Quote: ... Tyrosine hydroxylase was visualized using an Alexa Fluor® 594 conjugated mouse monoclonal anti-TH antibody at a 1:500 concentration (818004; Biolegend). Glutamine synthetase was visualized using a mouse monoclonal anti-GS antibody at a 1:100 concentration (MA5-27750 ...
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bioRxiv - Immunology 2022Quote: ... K562 cells were settled with vehicle-treated or Verbenalin-treated NK cells in the presence of Brilliant Violet 421™ anti-human CD107a (LAMP1) antibody (Biolegend) and GolgiStop™ (BD Biosciences) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42; 30-F11; 155837; BioLegend) was used for aortic cells of TRAF-STOP treated mice ...
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bioRxiv - Cancer Biology 2023Quote: ... Later 25µl of the following mixture of fluorescent-labeled antibodies was added to the samples: CD3-APC (Biolegend, San Diego, CA), CD4-BV510 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... All labelling was carried out at room temperature (RT) for 10mins protected from light with antibodies diluted in cell staining buffer (Biolegend; 420201). For AnnexinV staining ...
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bioRxiv - Immunology 2023Quote: ... and 7.5 nM of either a) tail-biotinylated cognate pMHC complex or b) biotinylated Armenian hamster anti-CD3ε antibody (clone 145-2C11, Biolegend 100304). ICAM-1 and pMHC proteins were expressed and purified as described (Wang et al. ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Cancer Biology 2023Quote: Cells were stimulated with IFNγ for 4h and the trypizinized cells were immunostained with VCAM1 antibody (1:100 dilution, BV421; BioLegend #305815). eBioscience™ Fixable Viability Dye eFluor™ 780 was used for discriminating dead cells ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of samples incubated with myeloid antibody cocktail were washed and resuspended in PBS for flow cytometry run whereas lymphoid antibody cocktail-stained cells were incubated overnight with 1 mL perm buffer (Biolegend; 421402) containing 10 μL of anti-FoxP3 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and preincubated for 15min with anti-CD16/CD32 Fc-block antibody (10μg/ml; BioLegend, San Diego, CA, 1:50) in FACS buffer followed by a 30min incubation with fluorescence dye-conjugated ...
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bioRxiv - Microbiology 2023Quote: ... Immunoblotting was performed as described previously30. Antibodies against Mtb Rho (a gift from D. Schnappinger, Weill Cornell Medicine) and against Eco RpoB (BioLegend, W0023) were used.