Labshake search
Citations for BioLegend :
5151 - 5200 of 10000+ citations for Mouse Anti Cytomegalovirus Phosphoprotein 65 Antibody 0885 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Suspensions were transferred to a round-bottomed 96-well plate and were resuspended in a cocktail consisting of an Alexa Fluor (AF) 647-conjugated anti-GFP antibody (BioLegend) and a eFluor 780 fixable viability dye diluted in FACS buffer ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then washed and stained for 30min at 4°C in the dark with Fc block diluted 1:200 and subsets of the following antibodies: anti-CD103 (2E7, Biolegend), anti-CD11b (M1/70 ...
-
bioRxiv - Immunology 2024Quote: ... for 40 min on ice or with biotinylated anti-MR1 antibody 26.5 (BioLegend, custom conjugation, used at 5 μg/ml) for 40 minutes on ice ...
-
bioRxiv - Immunology 2024Quote: ... plates were coated per well with 100 µL of anti-IgE capture antibody (2 µg/mL in PBS, clone RME-1, BioLegend) overnight at 4⁰ C ...
-
bioRxiv - Immunology 2024Quote: ... Efficiency of CD4+ T cell depletion was routinely assessed in small amounts of peripheral blood on d0 prior to LCMV challenge by flow cytometry staining with a non-competitively binding anti-CD4 antibody (clone RM4-4, Biolegend).
-
bioRxiv - Immunology 2024Quote: ... were isolated ex vivo from mouse peritoneal cavity by intraperitoneal lavage followed by magnetic bead-based enrichment for c-Kit+ cells using anti-CD117 (c-Kit)-APC antibody (Biolegend) and APC microbeads (Miltenyi Biotec) ...
-
bioRxiv - Cancer Biology 2024Quote: ... mesothelioma cell lines were further pre-treated with anti-MHC class I antibody (10 μg/ml) (Clone W6/32, Biolegend) for 1 hour ...
-
bioRxiv - Cancer Biology 2024Quote: ... NK cells were analyzed by flow cytometry for expression of NKG2A using above mentioned protocol but with anti-human NKG2A PECy5 antibody (Biolegend).
-
bioRxiv - Cell Biology 2023Quote: ... Cell surface expression level of syndecan-1 was evaluated by incubating cells with anti-syndecan-1 antibody (10 µg/ml, B280312, Biolegend) for 1h at 4 °C ...
-
bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
-
bioRxiv - Systems Biology 2023Quote: ... Cells were precooled for 20 min at 4 °C and then incubated with HDL-SOG for 40 min at 4 °C with 50 μg HDL per ml PBS or with anti-SCRB1 antibody-SOG (BioLegend, 363201 ...
-
bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
-
bioRxiv - Immunology 2023Quote: CAR-NK92 cells and NK92 cells were suspended with fresh culture medium supplied with 2 nM monensin and anti-CD107a-PE antibody (BioLegend) (1:1000 dilution) ...
-
bioRxiv - Biochemistry 2023Quote: ... The NMM/DMSO was incubated for 40 hours at 4°C while the primary antibody anti-tubulin beta- III (#802001, BioLegend), diluted at 1:2000 in 0.15% Triton-X in PBS ...
-
bioRxiv - Developmental Biology 2023Quote: ... When staining for SLAM markers the same lineage cocktail was used (FITC) with the addition of the following antibodies: anti-CD48 (FITC, clone HM48-1, BioLegend), anti-CD41 (FITC ...
-
bioRxiv - Cancer Biology 2023Quote: ... and then incubated for one hour in PE anti-human CD71 Antibody in the dark at 4°C (1:100; Biolegend). Cells were washed twice with PBS and then and then analysed using a flow cytometer (FACsFortessa ...
-
bioRxiv - Microbiology 2023Quote: ... for 1 hour at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with the following fluorochrome-conjugated antibodies: APC/Fire™ 750 anti-human CD324 (E-Cadherin) (Biolegend 324122), PE anti-human CD326 (EpCAM ...
-
bioRxiv - Immunology 2023Quote: ... Cells were washed with cold PBS and then stained with a cocktail of fluorescently labeled antibodies including: anti-human CD3-PE-Cy7 (Clone: OKT3, Biolegend), Ghost viability dye (Tonbo Biosciences),TCR a/b-BV421 (Clone ...
-
bioRxiv - Synthetic Biology 2023Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309 ...
-
bioRxiv - Immunology 2023Quote: ... We stably expressed these plasmids in OCI-Ly8 cells and used fluorescence activated cell sorting (FACS) to enrich for cells that stained negative with PE anti-human CD20 antibodies (clone 2H7, Biolegend). This sorted CD20-negative cell population were the OCI-Ly8 CD20KO cells.
-
bioRxiv - Cancer Biology 2023Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
-
bioRxiv - Genomics 2022Quote: ... The custom oligos were synthesized by Integrated DNA Technologies and conjugated to anti-Nuclear Pore Complex Proteins antibody (Clone Mab414, BioLegend) by BioLegend ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed three times with FACS buffer (PBS+0.5% BSA+2% FBS+0.1% sodium azide) and stained with the following antibodies for 30 minutes: anti-CD4 PerCP/Cy5.5 (BioLegend Cat: 100540), anti-CD8a Brilliant Violet 510 (BioLegend Cat ...
-
bioRxiv - Genomics 2023Quote: ... The cells were then incubated on ice for another 30 min after adding 0.5 μl of biotin anti-human CD15 antibody (BioLegend, 301913). After immunostaining ...
-
bioRxiv - Immunology 2023Quote: ... and goat anti-rabbit Alexa Fluor Plus 647-coupled secondary antibody (1:500) in Intracellular Staining Permeabilization Wash Buffer (Biolegend). The fraction of cells positive for cleaved caspase-3 was measured with a BD LSRFortessa SORP flow cytometer ...
-
bioRxiv - Cancer Biology 2023Quote: ... Antibodies used to distinguish the different cell populations included Brilliant violet 421 anti-human CD19 (Biolegend, San Diego, CA, USA), PE anti-human CD45 (Biolegend) ...
-
bioRxiv - Immunology 2023Quote: ... Lung resident cells were distinguished from those in the vasculature by exclusion from staining with fluorescently labeled anti-CD45 antibody (PerCP, clone 30F-11, BioLegend) that was injected retro-orbitally into each mouse approximately 3 minutes prior to euthanasia65 ...
-
bioRxiv - Cancer Biology 2023Quote: ... and incubated for 30 minutes on ice with the following antibody: anti-CD44 APC/Fire 750 (1:100, Biolegend, 103061), anti-CD24 PE (1:20 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The percentage of purified CD34+ cell was analysed by flow cytometry staining with anti-human CD34 PE antibody (BioLegend, UK). The cells were cultured in StemSpan SFEM II medium (StemCell Technologies ...
-
bioRxiv - Genetics 2023Quote: ... and 72 h by immunofluorescent staining with a PE-conjugated anti-human GPA primary antibody (BioLegend, San Diego, CA, USA) using flow cytometric analysis.
-
bioRxiv - Immunology 2023Quote: ... thawed PBMCs were FACS-sorted to isolate CD8+ CD4- CD56- cells (using BV785-conjugated anti-CD8 antibody, clone SK1 (Biolegend); PerCP-Cy5.5-conjugated anti-CD4 antibody ...
-
bioRxiv - Immunology 2023Quote: ... For comparison of resident vs non-resident populations tonsil samples were divided into CD69+ and CD69- subsamples using biotinylated anti-human CD69 antibody (FN40, Biolegend), and separation was performed using anti-Biotin Microbeads (Milenyi ...
-
bioRxiv - Immunology 2023Quote: ... the following antibodies were used at a 1:200 ratio: epithelial cells (324216, Biotin anti-human Epcam, clone:9C4, BioLegend), endothelial cells (13-0319-82 ...
-
bioRxiv - Cancer Biology 2023Quote: ... mice were injected intraperitoneally with three hundred micrograms of Ultra-LEAF purified anti-Asialo GM1 rabbit polyclonal antibody (clone Poly21460, BioLegend) every four days for the duration of the experiment ...
-
bioRxiv - Immunology 2024Quote: ... low endotoxin (Super affinity) (Acro) followed by secondary staining with APC conjugated anti-human IgG Fc Antibody (Biolegend, cat# 410712) and GFP/RFP expressions ...
-
bioRxiv - Immunology 2024Quote: ... cells were centrifuged and then resuspended in FACS buffer containing an antibody cocktail consistent of anti-CD11b AF488 (101217; Biolegend), anti-CD45 APC/Cy7 (157618 ...
-
bioRxiv - Immunology 2024Quote: ... It exhibits monocyte morphology and was originally derived from malignant cells obtained from the pleural effusion from a patient with histiocytic lymphoma.77 LFA-1 (CD11a/CD18) was assessed with a FITC anti-human antibody (clone m24, Biolegend), and MAC-1 (CD11b/CD18 ...
-
bioRxiv - Neuroscience 2024Quote: ... This process was repeated once and then cells were resuspended in the antibody solution containing APC anti-rat CD45RA (Cat# 202313, Biolegend) and FITC anti-rat CD3 (Cat# 201403 ...
-
bioRxiv - Cancer Biology 2024Quote: ... and incubated for 30 min at 4°C with 1 µL of PE anti-human FasL antibody (clone NOK-1) from BioLegend. Nonspecific background signals were measured by incubating cells with the appropriate isotype-matched antibody ...
-
bioRxiv - Cancer Biology 2024Quote: Single cell suspensions from tumors were washed with HBSS containing 2% FBS and 1 mM EDTA and stained with the following antibody cocktails for detecting lymphoid populations: anti-CD45-PerCP/Cy5.5 (BioLegend 103132), anti-NK1.1-FITC (BioLegend 108706) ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were then stained with Brilliant Violet (BV) 421TM anti-human CD138 (Syndecan-1) antibody (BioLegend catalog #356516, clone MI15), FITC anti-human HLA-A ...
-
bioRxiv - Microbiology 2024Quote: ... and used to transduce K562 cells as described below in “Lentiviral production and transduction.” Transduced cells were stained using anti-DCSIGN antibody (Cat #330105, Biolegend) and cell populations highly expressing both DCSIGN and BFP were bulk sorted (Sony MA900).
-
bioRxiv - Immunology 2024Quote: ... LAD2 cells were washed and incubated with the reaction products or serum-purified C3 or C3a (100 nM) along with a PE-conjugated anti-LAMP1 antibody (H4A3, Biolegend) for 1 h at 37 °C ...
-
bioRxiv - Immunology 2024Quote: ... Sections were saturated with BSA 2% PBS and then incubated overnight with an APC-conjugated anti-CD45 antibody (clone 30F11, Biolegend) at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2, BioLegend, cat. 302933) in R10 medium [RPMI-1640 medium supplemented with 10% heat-inactivated FBS ...
-
bioRxiv - Immunology 2024Quote: ... cells from each well were washed thrice with PBS and stained with APC/Cyanine7 anti-human CD14 Antibody (BioLegend, #123317) to assess THP-1 cell phagocytosis of HER2-positive tumor cells ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed with 3ml flow buffer (PBS with 2% FBS) at 300xg and stained with goat anti-mouse IgG-APC (BioLegend, clone Poly4053, catalog # 405308) and rat anti-mouse IgM-PE (ThermoFisher ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: Isolated aortic single cells were stained with Fc receptor blocker (TruStain FcX anti-mouse CD16/32; 101319; BioLegend, San Diego, CA, USA, 1:100) for 10 min at room temperature ...
-
Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... The slides were washed with PBS and incubated for 4 hours at room temperature with rat anti- mouse Ly6G (cone 1A8, BioLegend, San Diego, CA, USA). Slides were washed with PBS and incubated separately with Alexa Fluor 568 goat anti-rabbit (Invitrogen ...