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Citations for BioLegend :
5101 - 5150 of 10000+ citations for Mouse Anti Cytomegalovirus Phosphoprotein 65 Antibody 0885 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and finally incubated overnight at 4°C with primary antibodies against NKCC1 (T4, 1:1000, DSHB) and tubulin (anti-Tuj1, 1:5000, Biolegend) diluted in the blocking buffer with 0.1% tween ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Cancer Biology 2019Quote: ... Antibodies used to evaluate hypoxic status and surface marker expression were AlexaFluor 647 conjugated anti-HIF-1α (359706, Biolegend, CA), APC-Cy7 conjugated anti-CD8 (344714 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 2 was used to identify DE cells and contained 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Cocktail 1 was used to identify CD3+CD14+ cells and contained 3 μl of anti-human CD3-AF700 antibody (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE antibody (clone 61D3 ...
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bioRxiv - Immunology 2021Quote: IFNAR1 on WT and Mt3-/- BMDMϕ was neutralized using 10 μg/ml monoclonal anti-IFNAR1 antibody (BioLegend; Clone: MAR1-5A3) 1h prior and 24h after iLPS (10 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were sorted by fluorescent assisted cell sorting on the SH800S cell sorter (Sony Biotechnology) using the following antibodies: FITC anti-human lineage cocktail (Cat# 343604, BioLegend) and APC anti-human CD34 (Cat# 343509 ...
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bioRxiv - Bioengineering 2020Quote: ... The following antibodies and dyes were used: anti-human HLA-A,B,C Pacific Blue (clone W6/32, BioLegend®), anti-HA Alexa Fluor 647 (clone HA.11 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of protein (1/10 of input transferred in a new tube) was incubated together with 1 μL of anti-HA antibodies (BioLegend 901502 anti-HA.11 clone 16B12 ...
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bioRxiv - Developmental Biology 2022Quote: ... MGs were localized using the mT/mG reporter or stained using a rabbit anti-K14 polyclonal antibody (1:500, 905301, BioLegend). Nuclei were counterstained with DAPI ...
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bioRxiv - Pathology 2022Quote: ... Cells were blocked with anti-CD16/CD32 (eBioscience) and were stained with fluorochrome-conjugated monoclonal antibodies (eBioscience, BD, and BioLegend). For absolute quantification ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated for 20 minutes at RT with mouse PE conjugated 5-OP-RU MR1-tetramer (NIH Tetramer core) and the following antibodies: anti-CD4-FITC/APC-Fire810 (clone GK1.5, Tonbo Biosciences/Biolegend), anti-CD8α-AF700 (clone 53-6.7 ...
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bioRxiv - Immunology 2022Quote: ... Subsequent iterative cycles of standard immunohistochemistry were performed using the following anti-human primary antibodies: podoplanin (D240, BioLegend; 1:100), LYVE-1 (rabbit polyclonal ...
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bioRxiv - Immunology 2019Quote: ... the cells were thawed and transferred to a 96-well plate and stained for viability with Viability Dye eFluor 506 (eBioscience) followed by incubation with the surface antibodies: anti-human CD3 AF700 (clone UCHT1, Biolegend), CD4 BV785 (clone OKT4 ...
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bioRxiv - Immunology 2019Quote: ... cells were sorted from PBMC using a BD FACSAria cell sorter after staining with anti-human CD4 PE-conjugated antibody (Cat. #317410, Biolegend) or separated by human CD4+ T cell isolation kit (Cat ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1-2 million HL-60 and THP-1 cells were resuspended in PBS containing 1% fetal bovine serum and stained with PE anti-human CD11b antibody (Biolegend). Stained cells were analyzed on FACScalibur flow cytometer (Becton Dickinson) ...
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bioRxiv - Immunology 2019Quote: Enriched CD8+ were co-cultured with THP1 cells loaded with fixed E.coli at 30 BpC in the presence or absence of 20ug/mL LEAF™ anti-MR1 Antibody (Biolegend). Supernatants were collected at 72 hours ...
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bioRxiv - Immunology 2019Quote: ... The following antibodies were used for extracellular and intracellular staining as 2 different panels: anti-CD3 Alexa700 (clone UCHT1; BioLegend), anti-CD3 PerCp Cy5.5 (clone UCHT1 ...
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bioRxiv - Immunology 2019Quote: ... PBMC were then added to individual wells in AIM-V in the presence or absence of anti-CD28 antibody (Biolegend), IL-2 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel anti-parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with a 1:100 dilution of biotin anti-human IgG Fc antibody (clone HP6017, BioLegend) for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088 ...
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bioRxiv - Immunology 2019Quote: ... Blots were blocked 5% BSA in 1x TBS buffer 20mM Tris-HCL/150mM NaCl pH 7.6 for 2 hours and were subsequently incubated with anti-phosphotyrosine secondary antibody (Biolegend; 309302) at 1:1,000 in 5% BSA in TBS ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently stained for 1 hr at room temperature with anti-H2AX-Phosphorylated (Ser139) antibody conjugated with Alexa Fluor 647 (Biolegend), or Anti-p-CHK1 antibody conjugated with PE (Cell Signaling ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411 ...
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bioRxiv - Bioengineering 2020Quote: ... S1 and N+RBD were detected with anti-DYKDDDDK antibody (cat. no. 651503, BioLegend, for N, RBD or N+RBD), anti-His antibody (for S1 ...
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bioRxiv - Cancer Biology 2020Quote: ... and labelled via incubation with 1:100 dilution of Alexa Fluor 488 conjugated anti-CD45 antibody (Cat. no. 304017, BioLegend) and 1:100 dilution of PECy7-conjugated anti-CD31 antibody (Cat ...
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bioRxiv - Cell Biology 2020Quote: Single cell suspension was generated from adult lungs and stained with antibodies: anti-EpCAM (APC-Cy7-conjugated, Biolegend,1:50), CD49F (APC-conjugated ...
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bioRxiv - Cell Biology 2020Quote: ... The virus particles were stained at a concentration of 5×108 particles mL−1 and labeled with anti-GFP PE antibody (Clone FM264G, BioLegend) at 0.4 μg mL−1 in a 100 μL staining volume for a minimum of 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... T-25 or T-75 flasks were coated with 5 µg/mL anti-CD3ε antibodies (clone 145-2C11; 100340, BioLegend) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2021Quote: ... from Miltenyi according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 µg/ml anti-human RP105 antibody clone (312907, BioLegend).
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bioRxiv - Immunology 2020Quote: ... Slides were incubated with monoclonal antibodies overnight at 4°C using anti-CD3 (100202, clone 17A2, BioLegend, San Diego, CA) to detect T-cells ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... PD-1 and CD25 expression and cell viability and cell expansion were quantified by live/dead staining and the following antibodies via flow cytometry: anti-TCRa/b-PE (306708, BioLegend), anti-CD3-AF700 (11-0039-42 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transferred to a round-bottomed 96-well plate and were incubated with an AF647-conjugated anti-GFP antibody (1:20, BioLegend) and fixable viability dye eFluor 780 (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with PBS followed by surface staining at 4 °C with directly conjugated antibodies (see list below) diluted in FACS buffer containing anti-CD16/32 (BioXCell, BE0307, RRID:AB_2736987 or BioLegend, 101320, RRID:AB_1574975) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... Co-cultures were fixed at 7-10 days in either 4% paraformaldehyde for 10 minutes or ice-cold 70% ethanol for 30 minutes prior to staining with anti-CD31-AF488-conjugated antibody (BioLegend) and anti-VWF (DAKO ...
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bioRxiv - Biochemistry 2022Quote: ... The protein levels of overexpressed PRT6-WT and PRT6-136H+161V were determined by Western blot using anti-HA antibody (16B12, BioLegend).
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with Cell Viability Dye eFluor506 (eBioscience) and anti-CD45 antibody Pacific blue (clone 30-F11, Biolegend). CD45+ live cells were then sorted using BD Aria cell sorter.
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bioRxiv - Immunology 2022Quote: ... and then stained for 30 min on ice with the following antibodies in flow cytometry staining buffer (FACS): anti-CD4 AF589 (clone GK1.5; Biolegend #100446), anti-CD8b (clone YTS156.7.7 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with primary antibodies diluted in blocking solution: anti-βIII Tubulin (1/500, Biolegend), anti-RSK2 (1/100 ...
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bioRxiv - Cancer Biology 2022Quote: ... and supernatants used for HLA-I IP in which purified anti-human HLA-A,B,C antibody (W6/32, BioLegend) was crosslinked to Protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Systems Biology 2024Quote: ... Ultra-LEAF anti-human HLA-A,B,C antibody (clone W6/32, cat#: 311448) for immunopeptidomics was purchased from BioLegend. Polyethylenimine (PEI ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Immunology 2023Quote: VLP-producing Expi293F cells were extracellularly and intracellularly stained with Allophycocyanin (APC) anti-Flag (DYKDDDDK) tag antibody (1:500) (Biolegend) and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24 ...
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bioRxiv - Molecular Biology 2024Quote: ... Surface staining was carried out using a fluorochrome-labeled PE-conjugated anti-human CD11a (HI111) monoclonal antibody obtained from BioLegend.
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bioRxiv - Cell Biology 2024Quote: ... collected in PBS + 1% FBS and stained for ICAM-1 expression on the endothelial cell surface using APC anti-human CD54 antibody (353111, Biolegend). Flow cytometry analysis was performed and confirmed that ICAM-1 expression was upregulated following overnight stimulation with TNF in each of the co-culture experiments.