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Citations for BioLegend :
4951 - 5000 of 10000+ citations for Mouse Anti Cytomegalovirus Phosphoprotein 65 Antibody 0885 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were then stained using the following fluorescent antibodies: anti-CD45 FITC (BioLegend, San Diego, USA; Clone 30-F11), anti-CD19 Percp-Cy5.5 (eBioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... and genotyped for HLA*A:02 via flow cytometry with purified anti-human HLA-A2 antibody clone BB7.2 (BioLegend). HLA-A*02 positive samples were selected and dendritic cells were generated via plate adherence and pulsed with mHAs that are not endogenous to the sample (Peptide 2.0 Inc) ...
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bioRxiv - Neuroscience 2024Quote: ... RRID:AB_2315387) and axonal marker anti-neurofilament antibody (1:1000, Clone: SMI-312, # 837904, BioLegend, San Diego, CA, USA; RRID:AB_2566782) at 4°C overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... the cells were stained with live dye (Calcein AM) and PE anti-human CD45 antibody (BioLegend, cat no 368510) mixture ...
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bioRxiv - Physiology 2023Quote: ... Cell suspensions were incubated with the following antibodies for (20min, on ice): anti-ICAM1-PB (1:25, BioLegend, 322715); anti-VCAM1-PE (1:25 ...
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bioRxiv - Immunology 2024Quote: ... Then the cells were labeled as described above using antibodies anti-CD3-BV421 (clone 17A2) (BioLegend, SanDiego, CA, USA) (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated for 3 days using 3 μg anti-human CD3 and CD28 antibodies (Biolegend, 300438, 377604) and 50 ng/mL of IL-2 (Peprotech ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed three times with TBS-T (TBS with 0.1% Tween-20) and bound Anxs were detected by immunoblotting using Rat anti-FLAG antibody (1:5000; Biolegend) and ECL (ThermoFisher).
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bioRxiv - Immunology 2023Quote: ... PBMCs were seeded in 96-well plates and cultured alone or stimulated with anti-CD3 antibody (Clone: OKT3, Biolegend) or treated with 50 ng/ml IL-15 for 4 days ...
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bioRxiv - Immunology 2023Quote: ... CD141 BV605(Clone:M80 Cat:344117) in the presence of human anti-Fc Receptor antibodies (TrueStain; Biolegend; cat. no. 101320). cDCs1 were FACS-sorted as CD45+Lin- (Lin:CD3 ...
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bioRxiv - Cell Biology 2022Quote: ... MACS was performed in a two-step process using anti-F4/80-biotin coupled antibody (clone BM8, Biolegend, 123106) and Streptavidin microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Engineered T-cells were stained with increasing concentrations of AF647-conjugated anti-human TCR α/β antibody (IP26: Biolegend), HLA.A2/9V-AF647 or CD19-AF647 to determine the concentration of the staining agent leading to label saturation for CMV-specific RA14 TCR T-cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated with the L5 clone of Alexa Fluor® 594 anti-Flag antibody (BIOLEGEND INC, 637314), diluted 1:200 in PBS buffer containing 2% BSA ...
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bioRxiv - Immunology 2022Quote: ... enriched single-cell suspensions from tumor lysates were labelled with biotinylated anti-CD11b antibody (BioLegend, San Diego, CA, USA) and separated on MACS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... Slides were then blocked and incubated overnight at 4°C with anti-Lysozyme antibody diluted 1:400 (Biolegend, #860001). Slides were washed and incubated with anti-rabbit Alexa Fluor 555 diluted 1:500 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... blocked and stained as above with the following primary and secondary antibodies: anti-glutamine synthetase (1:100m, Biolegend #856201), anti c-Myc (9E10 ...
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bioRxiv - Neuroscience 2023Quote: ... IP of HA-positive ribosomes was performed by incubating with Anti-HA.11 Epitope Tag Antibody (Biolegend, Cat# 901515) for 4 hours in a microcentrifuge tube on a microtube rotator kept at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... At the end of the incubation cells were stained with PE anti-CD8 antibody (clone RPA-T8, Biolegend #301008) and the degranulation extent was measured among the GFP+ population as the percentage of CD8+ T cells expressing LAMP1 on their surface by flow cytometry analysis.
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bioRxiv - Cancer Biology 2023Quote: ... were coated with 50 µL per well of Purified anti-human IFNγ Antibody (clone MD-1; #507502, BioLegend, USA) diluted 1:200 in 1X PBS (#5460-0023 ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin-Streptomycin and IL-2 (100 IU/ml) and activated for 24 h in the presence of plate-bound anti-CD3 antibody (2.5 µg/mL; BioLegend) and soluble anti-CD28 antibody (2.5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in the dark for 15 min at 4°C with fluorochrome-conjugated antibodies in PBS containing anti-CD16/CD32 (2.4G2; BioXcell or BioLegend). Cells were washed and ...
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bioRxiv - Immunology 2023Quote: ... Primary and conjugated antibodies used in this study were: Armenian hamster anti-CD11c pacific blue (Biolegend, 1:100, #117321), goat anti-HIF-1α (R&D Systems ...
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bioRxiv - Bioengineering 2023Quote: ... The 5x diluted streptavidin-coated nanovial suspension was then incubated with an equal volume of 71.5 μg/mL of biotinylated anti-human VEGF antibody (Biolegend) for 30 minutes at room temperature on a tube rotator and washed ...
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bioRxiv - Bioengineering 2023Quote: ... This suspension was incubated with an equal volume of 71.5 μg/mL of oligo-barcoded anti-VEGF-A detection antibody (Biolegend) for 30 minutes at 37°C with gentle vortexing every 10 minutes before washing excess antibody with one high-dilution wash (>100x nanovial volume) ...
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bioRxiv - Cell Biology 2023Quote: ... Osaka University) were pre-incubated with anti-human-Fc secondary antibodies fused to APC (BioLegend, #410712, dilution 1:100) for 1 h in 1% BSA in PBS ...
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bioRxiv - Microbiology 2023Quote: ... for 1h at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then fixed in 3.7% formaldehyde for 6 min and PM HA-GLUT4-GFP detected by an anti-HA antibody (901503, BioLegend). HA staining was visualized by Cy3-goat anti-mouse secondary antibody (115-165-062 ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were incubated with a primary rabbit anti-MMP2 antibody (cat# 87809, BioLegend, San Diego, CA; 1:100) overnight ...
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bioRxiv - Immunology 2023Quote: ... Each B cell sample was split into two aliquots of at least 500,000 cells for labeling with different TotalSeqC anti-human cell hashing antibodies (BioLegend). Cells at a concentration of 5 million/ml were first incubated with human Fc block (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... the Rpb3 level was determined as a loading control by using an anti-Rpb3 monoclonal antibody (clone 1Y26; Biolegend). ECL reagents were used for protein detection ...
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bioRxiv - Cancer Biology 2023Quote: ... C57BL/6J mice bearing orthotopic KPC tumors were treated with 10 μg g−1 anti-TNF-α antibody (BioLegend) in combination with 100 mg/kg gemcitabine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed with ice cold PBS and surface stain with primary anti-PD-L1 (1:50) antibody (BioLegend) on ice for 90 min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were labelled with rat-anti-Prominin-1 antibody (Brilliant Violet 421-conjugated; BioLegend, #141213, Lot# B255870, 1:1000) and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... were given by tail-vein intravenous (i.v.) injection in 200 μL of PBS with 1 μg/mL of anti-CD3 antibody (OKT3, Biolegend). Monthly post HPC transplant ...
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bioRxiv - Cell Biology 2024Quote: ... The sliced sections were incubated with an anti-HA monoclonal antibody (1:1000; BioLegend, San Diego, CA, USA, #901514), followed by anti-mouse Alex Fluor 488-conjugated immunoglobulin (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Pluripotency marker expression was assessed every 10 passages by flow cytometry (PE anti-human SSEA-4 antibody, BioLegend, #330405) or RT-qPCR for DNMT3B ...
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bioRxiv - Bioengineering 2024Quote: ... Cells from each sample were labeled with TotalSeq™-B0251-0254 anti-human Hashtag Antibodies (clone LNH-94, Biolegend, RRID ...
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bioRxiv - Immunology 2024Quote: ... 500ul of fresh cell culture media for each respective cell type were combined with 10 µl of 0.5ug/µl purified anti-human ICAM-1 blocking antibody (Clone HA58, BioLegend), added to the wells ...
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bioRxiv - Immunology 2024Quote: ... and mesenteric lymph nodes were first treated with Fc receptor block (anti-CD16/CD32 clone 2.4G2) and then stained using the following fluorochrome-conjugated antibodies purchased from Biolegend, eBioscience ...
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bioRxiv - Immunology 2024Quote: ... they were stained with 1 uL each of the following TotalSeqC surface antibodies: anti-human IgM (BioLegend, Cat#314547), anti-human IgD (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... P75 and PDGFRβ primary antibody were revealed with the anti-rabbit phycoerythrin fluorochrome-conjugated (PE, poly4064, BioLegend, 406408, RRID:AB_10643424) and the anti-rat Alexafluor 488 fluorochrome-conjugated antibodies (AF488 ...
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bioRxiv - Immunology 2021Quote: ... and then stained with the fluorochrome-labeled anti-mouse CD3/CD4/CD8/CD44/CD62L mAbs (all purchased from Biolegend except CD44 from BD). Cells were then treated with Cytofix/Cytoperm (BD ...
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bioRxiv - Bioengineering 2021Quote: ... 1×106 P14 or pmel splenocytes were co-stained with Db-GP33 liposomes (10 μg/ml) and anti-mouse CD8-PE (Biolegend, Clone: 53-6.7) for 30 min on ice ...
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bioRxiv - Bioengineering 2021Quote: P14 CD8+ T cells were isolated as described above and incubated with DiO-labeled Db-GP33 liposomes and anti-mouse CD8 (Biolegend, Clone: 53-6.7) at 4°C or 37°C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Axons were identified using a staining against the phosphorylated neurofilaments (SMI31) stained with the PAB mouse-anti-SMI31 (Biolegend, cat. 801601, 1:250) and the SAB GAM-STAR580 (Abberior ...
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bioRxiv - Microbiology 2022Quote: ... single cell suspensions from mouse spleens were stained with fluorescein isothiocyanate (FITC)-conjugated anti-Ly-6A/E (Sca-1) mAb (Biolegend; San Diego, CA). To analyze erythrocyte maturation ...
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bioRxiv - Cell Biology 2021Quote: ... and the nerve sections were incubated for 24 hours at room temperature with gentle rocking: mouse anti-TUJ1 for microtubules in the axons (1:1000, Biolegend Catalog No. 801202), rat anti-PECAM-1 for endothelial cells (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...
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bioRxiv - Microbiology 2022Quote: ... The levels of serum cytokines and chemokines were determined by a bead-based multiplex assay using the LEGENDplex mouse anti-virus response panel (13-plex) kit (#740622, Biolegend, San Diego, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...