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Citations for Roche :
351 - 400 of 700 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: Samples of cDNA prepared from mouse tissues were analyzed either with a LightCycler instrument (Roche) with glass capillaries ...
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bioRxiv - Cell Biology 2019Quote: ... the tissue was permeabilized by incubation in proteinase-K (20 ng/ml K-03115836001 Roche) for 10 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... secondary lymphoid tissues were harvested and incubated with 10 mg/ml Collagenase D (Roche #11088866001) in plain RPMI medium (Gibco #11875093 ...
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bioRxiv - Immunology 2020Quote: ... Tissue was then digested for 30 minutes in RPMI containing 0.1mg/ml Liberase TL (Roche) and 50ug/ml DNase I (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... Viability of the tissue was assessed by a LDH Cytotoxicity Detection Kit (Roche, Mannheim, Germany) according to manufacturer’s instruction.
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... 1.5mL per 0.5g tissue (4mg/mL dispase II [Sigma D4693], 10mg/mL collagenase B [Roche 11088831001] in Hams-F10 [Multicell ...
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bioRxiv - Genetics 2022Quote: ... Pulverized tissue was suspended in modified RIPA buffer supplemented with protease inhibitor cocktail (Roche, IN). Following sonication ...
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bioRxiv - Molecular Biology 2022Quote: ... Muscle tissues were minced and digested in type II collagenase and dispase B mixture (Roche). Digestion was neutralized by adding growth media containing F-10 Ham’s medium (ThermoFisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Tissue sections were then deparaffinized and rehydrated automatically using a BenchMark ULTRA autostainer (Roche Ventana) programmed to apply ...
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bioRxiv - Immunology 2022Quote: ... CNS tissues were cut and digested in DMEM with Collagenase A (1 mg/ml, Roche) and DNase I (0.1 mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... tissue samples were homogenized (20 strokes on ice) in TBS with protease inhibitors (Roche, Switzerland) using a Teflon homogenizer ...
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bioRxiv - Physiology 2023Quote: Muscle tissue was homogenised in tubes containing ceramic beads (MagNA Lyser Green Beads, Roche, Germany) and 1 ml Tri-Reagent (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... microgliosis and astrocytosis on the Ventana Discovery XT automated IHC staining machine (Roche Tissue Diagnostics) as described in(36) ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was immediately collected in RIPA buffer along with phosphatase inhibitor (Roche, Basel, Switzerland) and protease inhibitors (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... and 300 μL MagNA Pure 96 DNA Tissue Lysis Buffer (Roche Diagnostics GmbH, Mannheim, Germany). Homogenization took place using the FastPrep-24 5G Homogenizer (MP Biomedicals) ...
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bioRxiv - Neuroscience 2023Quote: ... the tissue was fragmented in the presence of Deoxyribonuclease I (DNase I) (Roche, Cat.: #11284934001). The cells were grown for 10-14 days in the Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2023Quote: ... tissues were minced and enzymatically dissociated with 1 mg/mL collagenase D (Roche Applied Science) and 25 U/mL DNase I (Fujifilm Wako ...
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bioRxiv - Neuroscience 2023Quote: ... DRG tissues were digested for 50 min with 0.025 % collagenase A (cat no. 10103586001 Roche), 1 mM EDTA in PBS at 37°C under constant agitation and triturated at 20 ...
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bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated and minced tissue was digested with ∼5 mg of Collagenase P (Roche) in 5 mL cold HBSS for 15-20 minutes ...
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bioRxiv - Immunology 2023Quote: ... The tissue was then digested with Liberase Blendzyme 2 (0.2 mg/ml, Roche Applied Science) and DNase I (20 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from tail tissue using the KAPA Mouse Genotyping Kit (KAPA Biosystems, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Visceral white adipose tissues were diced and digested with 0.2 mg/ml Liberase TL (Roche) and 0.5 mg/ml DNase I (Sigma ...
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bioRxiv - Immunology 2024Quote: ... Kidney tissue was enzymatically digested with collagenase D at 0.4 mg/ml (Roche, Mannheim, Germany) and DNase I (10 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... Total cell lysates from COS-1 cells 24 hr post transfection were prepared using 300 μl of KAc interaction buffer (Roche Applied Science). GST fusion proteins were bound to glutathione-Sepharose beads (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... The lysates were clarified by centrifugation and then incubated with primary antibodies or HA antibody conjugated beads (HA beads, Roche Applied Bioscience) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... MGC803 cells were harvested and used to prepare lysate with cell lysis buffer with 1× Protease Inhibitor Cocktail (REF04693159001, Roche, Mannheim, Germany). The lysate was incubated with Glutathione Sepharose beads (20 μL for one group ...
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bioRxiv - Cancer Biology 2020Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Cancer Biology 2020Quote: The cell lysates were extracted with RIPA lysis buffer that contained a protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche, Basel, Switzerland). Equivalent concentrations of protein were separated using gel electrophoresis and were then transferred to a PVDF membrane ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysates were harvested 20 hours post-infection (hpi) and suspended in RIPA buffer with protease and phosphatase inhibitors (Roche, Thermo Fisher). Samples containing 10-20 µg protein were boiled for 20 min in sample buffer ...
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bioRxiv - Microbiology 2020Quote: ... Total nucleic acid was extracted from the lysate using the Roche MagNA Pure Compact Nucleic Acid Isolation Kit I and the MagNA Pure Compact instrument (Roche, Indianapolis, Indiana). Dual-indexed sequencing libraries were prepared from the lysates using Nextera XT DNA Library Preparation Kit (Illumina FC-131-1096) ...
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bioRxiv - Cancer Biology 2022Quote: Whole-cell protein lysates were prepared by lysing cells in Radioimmunoprecipitation assay buffer (RIPA) supplemented with cOmplete Protease inhibitor (Roche, Basel, Switzerland) and PhosphStop (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction of the cell lysate was transferred to a tube containing 30 μL cOMPLETE His-Tag purification Ni-NTA resin (Roche, Basel, Switzerland) suspended in 500 μL buffer A (8 M urea ...
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bioRxiv - Microbiology 2020Quote: ... tissue sections were washed several times with washing buffers before incubation with NBT/BCIP substrate (Roche) from 30 min to overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... tissues were homogenized in EBC-lysis buffer containing 1 mM Complete protease inhibitors cocktail (Roche Diagnostics) and 1 mM protein phosphatase Inhibitor Cocktail I (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: The skin tissue was homogenized using complete EDTA free protease inhibitor cocktail (11836170001; Roche Diagnostics GmbH), phosSTOP phosphatase inhibitor (30498800 ...
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bioRxiv - Cell Biology 2022Quote: ... Brain tissue was minced with scissors and incubated in 4,000 U/ml of collagenase D (Roche) at 37°C for 30 minutes ...
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bioRxiv - Physiology 2019Quote: ... The tissue was homogenized in a glass homogenizer in isolation buffer supplemented with protease inhibitors (Roche). Homogenates were filtered through a 70μm mesh and nuclei were removed by centrifugation at 845xg for 10min at 4°C in a microcentrifuge ...
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bioRxiv - Biochemistry 2019Quote: ... tissue extracts with 1% Nonidet P-40 in TBS containing protease inhibitors (Complete, Roche Molecular Biochemicals) were mixed with an equal volume of 0.2 M Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: ... and a single-cell suspension was prepared by digesting the tissue in collagenase type A (Roche) and 25 μg/ml DNase (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Extraction of total DNA was performed using a DNA tissue isolation kit (Roche Diagnostics, Indianapolis, IN) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... The tissues were then minced and incubated with collagenase A (1.0 mg/ml in HBSS, Roche) in water bath shaker at 37°C for 45 mins ...
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bioRxiv - Biochemistry 2022Quote: ... Tissue fragments were digested using an enzyme cocktail containing 0.025% collagenase P (Roche, 1.5 U/mg) and 0.025% pronase (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... Tissue pellet was resuspended in a solution of HBSS with 1 mg/ml collagenase A (Roche) and 1 mg/ml DNase I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... Tissues were weighed and homogenized with zirconia beads in a MagNA Lyser instrument (Roche Life Science) in 1 mL of DMEM media supplemented with 2% FBS ...
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bioRxiv - Microbiology 2020Quote: ... The IHC assay was carried out on a Discovery ULTRA automated staining instrument (Roche Tissue Diagnostics) with a Discovery ChromoMap DAB (Ventana Medical Systems ...
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bioRxiv - Pathology 2022Quote: ... RNA from pulmonary arterial smooth muscle was extracted using the High Pure RNA Tissue Kit (Roche) following the manufacturer’s guidelines and the concentration determined using the Nanodrop 1000 spectrophotometer (ThermoScientific) ...
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bioRxiv - Immunology 2020Quote: Tissues were weighed and homogenized with zirconia beads in a MagNA Lyser instrument (Roche Life Science) in 1000 μL of DMEM media supplemented with 2% heat-inactivated FBS ...
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bioRxiv - Immunology 2019Quote: ... Tissue was transferred to C-tubes (Miltenyi) and digested using Liberase™ (Roche, 57.6 μg/ml) in the presence of DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... inguinal adipose tissue was digested in a PBS buffer containing 10 mg/mL collagenase D (Roche), 2.4 U/mL dispase II ...
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bioRxiv - Molecular Biology 2019Quote: ... The tissue fragments were digested in DMEM medium containing 1 mg/mL Collagenase D (Roche, Switzerland), 1 mg/mL Collagenase/Dispase (Roche ...