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Citations for Roche :
451 - 500 of 700 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Fresh tissue was initially cut into small pieces and digested with collagenase VI (2 mg/ml; Roche) for 30 minutes at 37°C ...
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bioRxiv - Pathology 2019Quote: Tissue samples were prepared by addition of water and an aliquot of 50X complete Protease inhibitor (Roche) to frozen brain samples (3X w/v ...
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bioRxiv - Physiology 2020Quote: Skeletal muscle tissue muscle was homogenised in tubes containing ceramic beads (MagNA Lyser Green Beads, Roche, Germany) and 1 ml Tri-Reagent (Invitrogen ...
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bioRxiv - Physiology 2020Quote: Liver and adipose tissue were lysed in ice-cold RIPA buffer containing a protease inhibitor mixture (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... hippocampal tissue was hand homogenized in 10x w/v RIPA buffer with protease and phosphatase inhibitors (Roche). Homogenates were centrifuged at 14,000 rpm for 30 minutes at 4°C ...
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bioRxiv - Genomics 2022Quote: ... ileum and skin samples using the High Pure RNA Tissue Kit (Cat. No. 12033674001; Roche, Rotkreuz, Switzerland), the concentration and quality of the RNA being measured on aa NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... and tissue was resuspended in 5l of ice-cold HBSS containing 0.4mg/ml collagenase P (Roche, #11213857001) and 10mg/ml DNase I (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell suspensions were prepared by digesting the tissue in a cocktail consisting of Liberase TL (Roche, 5401020001) and DNase I (Sigma ...
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bioRxiv - Immunology 2021Quote: ... mouse tissues were weighed and homogenized with zirconia beads in a MagNA Lyser instrument (Roche Life Science) in 1 mL of DMEM medium supplemented with 2% heat-inactivated FBS ...
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bioRxiv - Immunology 2020Quote: Tissue was harvested and incubated in a digestion cocktail containing 1 mg ml−1 collagenase A (Roche) and 30 μg ml−1 DNase I (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... Tissue pieces were washed with 10mM HEPES in PBS and transferred into 0.42mg/ml Liberase DT (Roche)/0.125 KU DNase1 (Sigma)/10mM HEPES in RPMI and incubation for 30 minutes at 37°C ...
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bioRxiv - Plant Biology 2021Quote: Tissues incubated with anti-KN1 were incubated in a 1:50 dilution of NBT/BCIP (Roche, #11681451001) in 0.05M MgCl2/TBS ...
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bioRxiv - Immunology 2023Quote: Cell pellet or tissue were lysed in RIPA lysis buffer containing 1× proteinase inhibitor cocktail (Roche, 5056489001), 2 mM PMSF and 1 mM DTT ...
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bioRxiv - Neuroscience 2023Quote: ... The soft tissues were digested away with a mixture of 0.25 mg/ml collagenase/dispase (Roche #10269638001) and 0.25 mg/ml hyaluronidase (Sigma Aldrich #H3884-100MG ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue samples were lysed in RIPA buffer supplemented with protease and phosphatase inhibitors (Roche, Cat# 04693159001, 04906837001) using TissueLyser II (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor tissues were minced in small pieces and digested using Liberase DH Research Grade (Roche, Mannheim, Germany). Afterwards ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was extracted from formalin-fixed paraffin-embedded (FFPE) tissue (High Pure FFPET DNA Isolation Kit (Roche)) following laser capture microdissection in samples with small islands of malignant cells ...
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bioRxiv - Microbiology 2023Quote: ... mechanically disrupted by a tissue homogenizer (Ika) in 1 ml of PBS with protease inhibitor cocktail (Roche) and spun at 10,000 x g for 10 min to pellet debris ...
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bioRxiv - Neuroscience 2023Quote: ... They were homogenized in 10 μl/mg tissue 0.32 M sucrose containing protease inhibitor (cOmplete Mini, Roche) then centrifuged at 17000 g at 4°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... and 4 g of tissue were digested by dispase/collagenase (Collagenase: 0.1U/mL, Dispase: 0.8U/mL, Roche) for 1 hour at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The tissue was minced finely in the digest containing RPMI with 0.1 mg/ml liberase CI (Roche) and 1mg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... tissue was finely minced and cell dissociation was performed using a mixture of liberase TH (Roche, 05401135001) and DNase-I (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... The minced tissues after the centrifuge were incubated with collagenase IV (2.5 mg/ml, ROCHE, Cat# 11088858001) at 37 °C with gentle rotation for 30 min in 2 ml of HBSS++ ...
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bioRxiv - Cell Biology 2023Quote: ... all tissue sections were stained with DAPI (4′, 6-Diamidine-2′-phenylindole dihydrochloride, #10236276001, Roche, 1:3000). For image generation an Axio Observer ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue fragments were then digested in DMEM medium containing 1 mg/mL Collagenase D (Roche, Switzerland), 1 mg/mL Collagenase/Dispase (Roche) ...
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bioRxiv - Physiology 2023Quote: ... cells/tissue were lysed in mammalian protein extraction reagent (M-PER) supplemented with protease inhibitor cocktail (Roche). Soluble proteins and insoluble matrix were separated by centrifugation at 10,000 × g ...
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bioRxiv - Molecular Biology 2024Quote: ... all tissue sections were stained with DAPI (4’, 6-Diamidine-2’-phenylindole dihydrochloride, #10236276001, Roche, 1:3000). Images were taken with a confocal microscope from Zeiss (LSM 880 Airyscan).
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bioRxiv - Neuroscience 2021Quote: Frozen brain tissues were homogenized on ice in RIPA buffer supplemented with protease inhibitor cocktail (Roche Applied Science) and phosphatase inhibitors (50nM okadaic acid ...
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bioRxiv - Molecular Biology 2020Quote: Frozen mouse tissues were homogenized in CelLytic M cell lysis reagent (MilliporeSigma) with cOmplete™ protease inhibitors (Roche). Tissue lysates were centrifuged at 10,000 × g for 5 min at 4 °C and supernatants were collected for further testing ...
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bioRxiv - Microbiology 2020Quote: ... Cells were seeded in 10 cm tissue-culture treated dishes and transfected with FuGENE 6® reagent (Roche) for 24 hr ...
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bioRxiv - Cell Biology 2021Quote: ... Stromal vascular fraction (SVF) cells and mature adipocytes were separated from whole adipose tissue biopsies following collagenase (Roche) digestion (1 mg/ml in Hanks’ buffered salt solution ...
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bioRxiv - Cancer Biology 2022Quote: ... The minced tissue was resuspended in DMEM supplemented with Liberase Thermolysin Medium enzyme mix (0.2 mg/ml, Roche) and DNAse I (0.2mg/ml ...
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bioRxiv - Neuroscience 2022Quote: Fresh frozen pulverized tissue was suspended in tris-buffered saline (TBS) containing cOmplete mini protease inhibitor tablet (Roche) at a concentration of 10% w/vol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... RNA from cell culture and mouse heart tissue was extracted using high pure RNA isolation kit (Roche, 11828665001) and miRNeasy Mini kit (Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: ... sections from FFPE tissue blocks were processed at the Mount Sinai Pathology core using a Ventana system (Roche).
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bioRxiv - Neuroscience 2019Quote: ... tissue was homogenized in 200μl Tris buffer (pH 7.4) containing 10% sucrose and protease inhibitors (Complete; Roche Diagnostics), sonicated and kept at −80°C until use ...
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bioRxiv - Cell Biology 2019Quote: ... Tissue was directly lysed in 2x Laemmli Sample Buffer containing 5% β-ME and a PhosSTOP tablet (Roche), 10 ul per embryo ...
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bioRxiv - Immunology 2020Quote: ... The remaining tissue was incubated in digestion buffer (RPMI 1640, 10% FCS, 1.25 mg/ml collagenase D (Roche), 0.85 mg/ml collagenase V (Sigma– Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... These cells were then lysed in T-PER tissue protein extraction reagent (Pierce) containing protease inhibitor cocktail (Roche) and phosphatase inhibitor cocktail 1(Sigma) ...
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bioRxiv - Genomics 2021Quote: ... brain tissue was snapped frozen and lysed in the RIPA lysis buffer containing cocktails of proteases inhibitors (Roche) and phosphatase inhibitors (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... was homogenized and 4 g of tissue were digested by dispase/collagenase (Collagenase: 0.1U/mL, Dispase: 0.8U/mL, Roche) for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells or tissues were collected and lysed in RIPA lysis buffer supplemented with Protease Inhibitor Cocktail (Roche, Germany) for 15 min on ice ...
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bioRxiv - Immunology 2021Quote: ... lung tissue was minced with a scalpel and digested enzymatically with 0.15 WU/mL of D-Liberase (Roche) and 800□U/mL of DNase I (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... OPCs were removed from the tissue culture plates gently with 0.2 Wünsch unit (WU)/ml of Liberase DH (Roche) and DNase I in PGM at 37°C for 5 min ...
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bioRxiv - Developmental Biology 2021Quote: ... and cell suspensions were prepared by digesting the tissue in a cocktail consisting of Liberase TL (Roche, 5401020001) and DNase I (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from 500 uL of nasal wash fluid using the high Pure RNA Tissue Kit (Roche) and DNA was amplified in the Light Cycler (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... the tissue was enzymatically digested for 45 minutes with dispase II as 2.4 U/ml (Roche, Basel, Switzerland) followed by further digesting the lamina propria with DMEM/F12 media containing 0.25 mg/ml collagenase H (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated from tissue samples using the TriPure Isolation Reagent (Roche Applied Science, Indianapolis, IN, USA). The RNeasy Mini Kit (Qiagen ...
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bioRxiv - Developmental Biology 2022Quote: The TUNEL assay was performed in cryosectioned lung tissue using the In Situ Cell Death Detection Kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Cells or tissue powders were directly dissolved in 1 ml of the TriPure Isolation Reagent (Cat. 11667165001, Roche) and extracted using a column-based kit (Cat ...