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Citations for Roche :
601 - 650 of 700 citations for Porcine Tissue Lysates since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cells and tissue were lysed with RIPA buffer (Beyotime Biotechnology) supplemented with PMSF (Beyotime Biotechnology) and protease inhibitor cocktail (Roche). Mettl3 (abcam ...
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bioRxiv - Neuroscience 2021Quote: ... 0.4-0.5 g of frozen microdissected tissue was thawed on wet ice and then immediately homogenized in 500 µl of PBS spiked with protease (Roche) and phosphatase inhibitors (Thermo Scientific ...
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bioRxiv - Bioengineering 2022Quote: Ileal and colonic proteins were obtained by tissue homogenization in NP-40 Buffer with 1x cOmplete Mini Protease Inhibitor Cocktail (Roche). Protein concentrations were determined using Bradford Assay ...
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bioRxiv - Immunology 2022Quote: ... tumor tissue was incubated at 37°C for 30minutes in FBS free DMEM media containing liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... Tissue fragments were dissected with sterile scissors in a FBS-free DMEM solution containing 1x Liberase TM Research Grade (Roche) and Antibiotic-Antimycotic (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was homogenized in a ‘homogenization buffer’ (Sucrose, 100 mM EGTA, 10 mM Tris, protease and phosphatase inhibitors (Roche), pH 8.1 ...
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bioRxiv - Cell Biology 2023Quote: Whole kidneys were homogenized and protein was extracted from 20 mg of tissue in 1 ml RIPA buffer supplemented with protease inhibitors (Roche) for 2 hrs ...
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bioRxiv - Immunology 2023Quote: Cells and tissues were lysed in NP-40 lysis buffer or SDS lysis buffer supplemented with protease inhibitor (Roche, 04693159001) and then centrifuged at 4 °C to obtain cellular lysate ...
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bioRxiv - Microbiology 2023Quote: Viral RNA was extracted from 100 µL of tissue or nasal wash samples using MagNA Pure 24 Total NA Isolation Kit on the MagNA Pure 24 system (Roche) and eluted with 50 µL of water ...
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bioRxiv - Plant Biology 2023Quote: ... untreated and chitosan treated tissue samples were ground in liquid nitrogen and dissolved in an extraction buffer containing Phosstop (Roche), and the concentration of proteins determined by the Bio-Rad protein assay kit with bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... tumor tissue was harvested digested with 1 mg/ml collagenase type I and 100 μg/ml DNase I (both Roche) and T cell infiltration analyzed by flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... One lobe of lung tissue was collected in an Omni Bead Ruptor tube filled with Tripure Isolation Reagent (catalog number 11667165001; Roche). Tissue was homogenized using an Omni Bead Ruptor 24 instrument (5.15 ms ...
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bioRxiv - Developmental Biology 2023Quote: The ovary and liver tissues were washed with PBS and lysed with RIPA buffer supplemented with protease inhibitors cocktail (Roche). Equal amounts of total protein were boiled in sodium dodecyl sulfate (SDS ...
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bioRxiv - Pathology 2023Quote: Total RNA was extracted from sigmoid colon tissue using a High Pure RNA Isolation kit (Roche Applied Science, Penzberg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... tumor tissue was dissected into approximately 1– 5 mm3 fragments and digested with collagenase Type D (2 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... based chromogenic IHC on tissue samples (FFPE or fresh frozen) was performed on an automated Ventana Discovery ULTRA system (Roche). Fresh frozen tissues were air dried at room temperature for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues were incubated in digestion buffer (0.5 ml HBSS plus 0.26 U/ml Liberase DH [Roche] and 1% sheep serum) for 45 minutes at 37°C while agitating at 750 rpm on an Eppendorf ThermoMixer ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... cells and mice tissue were collected and fragmented in lysis buffer (Beyotime, Shanghai, China) supplemented with protease inhibitors cocktail (Roche). Isolated proteins were then segregated by gel electrophoresis and transferred to polyvinylidene fluoride membranes (Millipore) ...
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bioRxiv - Neuroscience 2023Quote: ... pons and frontal cortex tissues were obtained by homogenization in cold phosphate-buffered saline (PBS) containing protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Immunology 2023Quote: ... Lung tissues were homogenized with a GentleMACS dissociator (Miltenyi) and digested with a cocktail of Liberase (70 μg/mL, Roche) and aminoguanindine (10mM ...
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bioRxiv - Cell Biology 2023Quote: Snap-frozen left ventricular tissue collected after the sacrifice of mice was homogenized in RIPA buffer with protease inhibitor cocktail (Roche) using prechilled Precellys bead homogenizer ...
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bioRxiv - Cancer Biology 2024Quote: DNA extracted from FFPE lung tissue samples was sequenced through an orthogonal method by targeted enrichment (Roche Avenio Targeted Assay) and sequencing (NextSeq 500 ...
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bioRxiv - Neuroscience 2023Quote: Protein extracts from DCN and frontal cortex tissues were obtained by homogenization of brain tissue in cold phosphate-buffered saline (PBS) containing protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Immunology 2023Quote: ... lung and liver tissues were mechanically disrupted into small pieces and enzymatically digested in 2ml of digestion buffer (1 mg Collagenase D (Roche) and 0.2 mg DNase I (Roche ...
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bioRxiv - Pathology 2024Quote: Tissues were homogenized using RIPA buffer and a TissueLyser in presence of protease and phosphatase inhibitors (Inhibitor cocktail (Roche, Switzerland) and 1μM sodium ortovanadate) ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorophores with the least autofluorescence on FFPE tissue were selected to minimize false positives: Cyanine 5 (Cy5) (DISCOVERY CY5 Kit, Cat#760238, Roche Diagnostics Corporation ...
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bioRxiv - Bioengineering 2024Quote: ... Primary fibroblast cultures were obtained by subjecting small pieces of mouse ear tissue to a 30-minute digestion with Collagenase/Dispase (Roche) at 37°C under agitation ...
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bioRxiv - Developmental Biology 2024Quote: Primary tissue samples from reduction mammoplasties of healthy women were dissociated through a 3 mg/mL collagenase (Roche Life Science) solution and sorted via density gradient ...
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bioRxiv - Zoology 2019Quote: ... TBST washes of 15 minute each at room temperature were performed thrice and subsequently the tissue was stained using NBT (working concentration 500ug/ml; Roche #11383213001) and BCIP (working concentration 562.5ug/ml ...
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bioRxiv - Genomics 2020Quote: ... tissue was cut into 1mm x 1mm cubes and placed in 0.25mg/ml liberase TH dissociation medium (Roche Diagnostics, Indianapolis, USA). Following further dissection ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from 10μm-sections of FFPE tissues using a commercial kit (High pure FFPET RNA isolation kit, Roche, Cat.6650775001) and resuspended in 25μl final volume ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated in enzyme mix for tissue dissociation (collagenase type II enzyme mix (Gibco, 17101-015, 5mg/ml dissolved in Basis medium, DNase: 15ug/ml (Roche, 10104159001) and 10 μM Y-27632-HCl Rock inhibitor (Selleckchem ...
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bioRxiv - Physiology 2021Quote: Tissues were homogenized in homogenization buffer (100 mM PMSF, 500 mM EDTA, EDTA-free Protease Inhibitor Cocktail (Roche, catalog number 11836170001), PBS ...
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bioRxiv - Cell Biology 2021Quote: Transduced MEFs were seeded in triplicate in a 96 well flat bottom tissue culture plate and WST-1 assay (Roche, Switzerland) performed as per manufacturer’s protocol at day 0 ...
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bioRxiv - Molecular Biology 2022Quote: ... synovial tissues from three patients with RA were minced and incubated with 1 mg/mL collagenase/dispase (Roche, Indianapolis, IN, USA) in phosphate-buffered saline (pH7.2 ...
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bioRxiv - Cell Biology 2022Quote: Murine cells or aortic tissue were lysed in RIPA buffer containing complete protease inhibitor and phosphoSTOP phosphatase cocktails (Roche Applied Science). Lysate was centrifuged at 10,000 g for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... We transferred the minced tissue to a tube containing 5 mL of digestion Buffer containing collagenase D (2mg/mL, Roche #11088858001) and DNase (0.125 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: TUNEL assay was performed on N153S and WT lumbar disc tissue sections using an in situ cell death detection kit (Roche Diagnostic). Briefly ...
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bioRxiv - Immunology 2021Quote: ... Pancreas and liver tissue extract were prepared in RIPA buffer containing a mixture of protease inhibitors (Complete protease inhibitor, Roche Diagnostics).
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bioRxiv - Bioengineering 2021Quote: ... and stained with hematoxylin and eosin at the UNMC Tissue Sciences Facility using automated Ventana Discovery Ultra (Roche Diagnostics, Indianapolis, IN) as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: HS and healthy skin tissues were cut into small pieces and were digested with 2.5U/ml Dispase-II (Roche, Cat#04942078001) in Hanks’ Balanced Salt Solution (HBSS ...
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bioRxiv - Neuroscience 2022Quote: ... and tissue homogenization was performed using a Bullet Blender in RIPA buffer supplemented with 1% cOmpleteTM protease inhibitor cocktail (Roche Diagnostics), 1% phosphatase inhibitor cocktail 2 (#P5726 ...
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bioRxiv - Neuroscience 2019Quote: ... mPFC tissue was incubated overnight at 4°C with an anti-DIG antibody coupled to horseradish peroxidase (Roche, Mississauga, ON, Canada) and mouse monoclonal anti-SMI-32 antibody (1:1000 dilution ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated in enzyme mix for tissue dissociation (collagenase type II enzyme mix (Gibco, 17101-015, 5mg/ml dissolved in Basis medium, DNase: 15ug/ml (Roche, 10104159001) and 10 μM Y-27632-HCl Rock inhibitor (Selleckchem ...
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bioRxiv - Cell Biology 2019Quote: ... Brain tissue was homogenized in a teflon dounce grinder on ice in PBS with protease and phosphatase inhibitors (Roche, Indianapolis, Indiana). After homogenization ...
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bioRxiv - Physiology 2019Quote: The expression pattern of Mr-GS in various tissues at different time points was studied using qRT-PCR in Roche LightCycler 480 (Roche, USA). All used primers were presented in Table 1 ...
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bioRxiv - Neuroscience 2021Quote: Tissue samples were homogenized by sonication in phosphate-buffered saline (PBS) mixed with a protease inhibitor cocktail (Complete®, Roche, Spain) (pH =7) ...
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bioRxiv - Cancer Biology 2022Quote: ... the frozen human tumors and normal tissues and the tumors from animal xenograft studies were lysed in RIPA lysis buffer containing protease and phosphatase inhibitors (Roche Inc), and sonicated for 20s ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues or cells were lysed in NP-40 lysis buffer (Beyotime P0013F) supplemented with protease inhibitor cocktail (complete Mini, Roche 04693124001), phosphatase inhibitor cocktail (PhosSTOP ...
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bioRxiv - Immunology 2022Quote: ... We transferred the minced tissue to a tube containing 5 ml of digestion buffer containing collagenase D (2mg/ml, Roche #11088858001) and DNase (0.125 mg/ml ...